Abstract:
:Numerous hydrogel-based culture systems are used to create in vitro model for prevascularization. Hydrogels used to induce a microenvironment conducive to microvessel formation are typically soft and fast degradable, but often suffer from maintaining a lasting perfusable channel in vitro. Here, a dual hydrogel system that consists of photo-crosslinkable gelatin methacrylate (GelMA) and polyethylene glycol dimethacrylate (PEGDMA) is reported. GelMA hydrogels present soft and rapidly degradable properties and show microporous structures while PEGDMA is relatively stiff, almost nondegradable in vitro, and less porous. The dual hydrogel system is sequentially photo-crosslinked to construct an endothelial cell (EC)-lined perfusable PEGDMA channel and surrounding GelMA for endothelial vascular networks. Such dual hydrogel system exhibits seamless integration of the stiff PEGDMA channel and the surrounding soft GelMA, and facilitates rapid EC sprouting and extensive microvessel formation from a stable endothelium on the PEGDMA channel into the GelMA. Furthermore, diffusivity of biomolecules in the perfusable dual hydrogel system is affected by both the structural and physicochemical properties of the hydrogel system and the microvascular networks formed in the system. The establishment of the dual hydrogel system for vascularization holds great promise as an in vitro angiogenesis model and prevascularization strategy of large tissue constructs.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Kim S,Pan CC,Yang YPdoi
10.1002/mabi.202000204subject
Has Abstractpub_date
2020-10-01 00:00:00pages
e2000204issue
10eissn
1616-5187issn
1616-5195journal_volume
20pub_type
杂志文章abstract::The aim of this study is to design a novel two-component hybrid scaffold using the fibrin/alginate porous hydrogel Smart Matrix combined to a backing layer of plasma polymerized polydimethylsiloxane (Sil) membrane to make the fibrin-based dermal scaffold more robust for the treatment of the clinically challenging pres...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201700185
更新日期:2017-11-01 00:00:00
abstract::The synthesis of a new glycomonomer based on mannose, prepared via CuAAC, is reported. The resulting 1,2,3-triazole linkage between mannose and the polymer backbone ensures the formation of highly stable glycopolymers, which will not undergo hydrolysis. The monomer 2'-(4-vinyl-[1,2,3]-triazol-1-yl)ethyl-O-alpha-D-mann...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200900199
更新日期:2010-02-11 00:00:00
abstract::Chronic non-healing wounds show delayed and incomplete healing processes and in turn expose patients to a high risk of infection. Treatment currently focuses on dressings that prevent microbial infiltration and keep a balanced moisture and gas exchange environment. Antibacterial delivery from dressings has existed for...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.201300561
更新日期:2014-06-01 00:00:00
abstract::The antimicrobial activity of poly(alkyloxazoline) telechelics with one quaternary N,N-dimethyldodecylammonium (DDA) end group was found to be greatly controlled by the non-bioactive distal end group, the so-called satellite group. In systematic investigations, the nature of the latter groups was varied to explore the...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200400169
更新日期:2005-02-23 00:00:00
abstract::The design of a novel polymer-modified overlayer composed of PPAPE and GPMS on a silicon wafer for immobilization of DNA molecules is described. After hydroxylation of Si(100) surfaces, GPMS molecules were self-assembled onto these surfaces. PPAPE molecules were then covalently attached to the epoxy-terminated surface...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200800226
更新日期:2009-05-13 00:00:00
abstract::pH-responsive amphiphilic graft macromolecules consisting of a polyphosphazene backbone, hydrophilic PEG branches and pH-sensitive DPA were successfully synthesized and characterized. The copolymer can self-assemble into vesicles in an aqueous solution with unique inner structure and homogeneously encapsulate both lip...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000333
更新日期:2011-03-10 00:00:00
abstract::In drug delivery to the human brain, blood vessels are a significant hurdle because they restrict the entry of most solutes to protect brain. To overcome this hurdle, an in vitro 3D model for brain endothelial barrier is developed using a microfluidic device with hydrogel providing a 3D extracellular matrix scaffold. ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201900425
更新日期:2020-06-01 00:00:00
abstract::Comb-shaped glycopolymer/peptide bioconjugates are constructed by grafting reduced glutathione (GSH) onto acrylate-functional block glycocopolymers via thiol-ene click chemistry. In aqueous solution, the glycopolymer/GSH bioconjugate self-assembles to sugar-installed spherical micelles. The size of micelles decreases ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200274
更新日期:2012-11-01 00:00:00
abstract::Mussel-inspired poly(dopamine) (PDA) coating is proven to be a simple, versatile, and effective strategy to promote cell adhesion onto various substrates. In this study, the initial adhesive behavior of human umbilical vein endothelial cells (HUVECs) is evaluated on a PDA coating under serum-free conditions. It is fou...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200390
更新日期:2013-04-01 00:00:00
abstract::Unique features of nanofibers provide enormous potential in the field of biomedical and healthcare applications. Many studies have proven the extreme potential of nanofibers in front of current challenges in the medical and healthcare field. This review highlights the nanofiber technologies, unique properties, fabrica...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.201800256
更新日期:2019-02-01 00:00:00
abstract::Shape memory polymers (SMPs) are a class of polymeric materials used in various medical interventions such as vascular stents. In this work, two SMPs, thermoplastic (TP) and thermoset (TS), have been measured in vitro for the degree of cellular and protein adhesion, their ability to stimulate inflammatory cytokine pro...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200600177
更新日期:2007-01-05 00:00:00
abstract::Cardiovascular diseases, including myocardial infarction, are the cause of significant morbidity and mortality globally. Tissue engineering is a key emerging treatment method for supporting and repairing the cardiac scar tissue caused by myocardial infarction. Creating cell supportive scaffolds that can be directly im...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201600446
更新日期:2017-07-01 00:00:00
abstract::Pedobacter sp. KP-2 can degrade and metabolize thermally synthesized alpha,beta-poly(D,L-aspartic acid) (tPAA), which contains 70% of unnatural beta-amide units, with high-molecular-weight. In this study, gene cloning and molecular characterization of PAA hydrolase-1 from KP-2 was carried out. Gene analysis reveals th...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200800106
更新日期:2009-01-09 00:00:00
abstract::Although recently a growing number of reports demonstrate that topography or geometry of the substrate also plays an important role in the fate of the stem cells, most of these studies are usually completed by a few distinct patterns such as simple lines, posts, etc. As a result, there is a lack of quantitative analys...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000477
更新日期:2011-07-07 00:00:00
abstract::Stereolithography-assisted fabrication of hydrogels of carboxybetaine methacrylamide (CBMAA) and a α,ω-methacrylate poly(d,l-lactide-block-ethylene glycol-block- d,l-lactide) (MA-PDLLA-PEG-PDLLA-MA) telechelic triblock macromer is presented. This technique allows printing complex structures with gyroid interconnected ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201800403
更新日期:2019-04-01 00:00:00
abstract::Conducting polymers (CPs) is one of intelligent biomaterials with the specific properties of reversible redox states, which have a significant effects on the cell behaviors and nerve tissue regeneration. However, the effects of CPs with different electrical conductivity on the behaviors of nerve cells are rarely repor...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201900147
更新日期:2019-10-01 00:00:00
abstract::Previously synthesized amphiphilic diblock copolymers with pendant dendron moieties have been investigated for their potential use as drug carriers to improve the delivery of an anticancer drug to human breast cancer cells. Diblock copolymer (P71 D3 )-based micelles effectively encapsulate the doxorubicin (DOX) with a...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201500435
更新日期:2016-06-01 00:00:00
abstract::The amyloid-β peptide (Aβ) plays a central role in the mechanism of Alzheimer's disease, being the main constituent of the plaque deposits found in AD brains. Aβ amyloid formation and deposition are due to a conformational switching to a β-enriched secondary structure. Our strategy to inhibit Aβ aggregation involves t...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200900448
更新日期:2010-10-08 00:00:00
abstract::The antiviral effects of the cationic acrylate polymer E100 on a panel of lipid-enveloped viruses and the interactions involved are studied. The treatment of several common viruses with E100 induced a dose-dependent inhibition of the infectivity of viruses below the detection limit of the assays employed. Similarly, t...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200700102
更新日期:2007-09-11 00:00:00
abstract::Hyperbranched polyurethanes are synthesized using TDI, PCL diol, butanediol, and pentaerythritol (1-5 wt%) as the B(4) reactant with and without the monoglyceride of sunflower oil. The biodegradation, physico-mechanical, and thermal properties are found to be tailored by varying the percentage weight of the branching...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200244
更新日期:2013-01-01 00:00:00
abstract::Photo-crosslinkable, fumaric acid monoethyl ester-functionalized triblock oligomers are synthesized and copolymerized with N-vinyl-2-pyrrolidone to form biodegradable photo-crosslinked hydrogels. Poly(ethylene glycol) is used as the middle hydrophilic segment and the hydrophobic segments are based on D,L-lactide, trim...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201100468
更新日期:2012-05-01 00:00:00
abstract::In this study we formed and characterized dynamic hydrogel microspheres in which a protein conformational change was used to control microsphere volume changes and the release of an encapsulated drug. In particular, a specific biochemical ligand, trifluoperazine, induced calmodulin's nanometer scale conformation chang...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200900382
更新日期:2010-06-11 00:00:00
abstract::Synthetic resins have been extensively employed by artists in their works of art, e.g. as paint binders, or by conservators for conservation treatments, e.g. as stone consolidants and protectives. It is generally thought that synthetic resins are less prone to chemical, physical and biological deterioration than other...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.200300055
更新日期:2004-04-19 00:00:00
abstract::Novel water-insoluble, and reduction-responsive nonwoven scaffolds were fabricated from gamma-PGA and tested in cell culture. An electrospinning method was developed to produce scaffolds of fibers with diameters of 0.05-0.5 microm. Crosslinking of the fibers with cystamine in the presence of EDC resulted in water-inso...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200800334
更新日期:2009-06-11 00:00:00
abstract::Cells in physiological 3D environments differ considerably in morphology and differentiation from those in 2D tissue culture. Naturally derived polymer systems are frequently used to study cells in 3D. These 3D matrices are complex with respect to their chemical composition, mechanical properties, and geometry. Theref...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.201200132
更新日期:2012-10-01 00:00:00
abstract::This study aims to develop an effective method to control motile microorganisms and enable their manipulation as functional 'live micro/nano robots'. A novel strategy based on Fe3 O4 nanoparticle-doped alginate hydrogel is developed to fashion an artificial extracellular matrix (ECM) for microbial cells (e.g., Sacchar...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201600143
更新日期:2016-10-01 00:00:00
abstract::Uniform stereo-complex microparticles ranging from nanometer to micrometer size are prepared by using stereo multiblock co-poly(rac-lactide)s (smb-PLAs) with different stereo-regularity. At comparable molecular weights, as the smb-PLA stereo-regularity decreases from 88% to 76%, the crystallinity of the microparticles...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200500157
更新日期:2005-12-15 00:00:00
abstract::The present study investigates the effect of galactosylated gelatin on encapsulated HepG2 cells. Methacrylamide modified gelatin is evaluated and compared with its galactosylated counterpart with respect to effects on viability, morphological characteristics, proliferation, and the expression of hepatocyte specific ma...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201300320
更新日期:2014-03-01 00:00:00
abstract::In this paper, a novel composite hydrogel was prepared by the use of dialdehyde konjac glucomannan (DAK) as macromolecular cross-linking agent for chitosan (CS). This biocompatible material cross-links and gels in minutes. The structure and morphology were characterized by various analyses. The results indicate that t...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200700035
更新日期:2007-09-11 00:00:00
abstract::The biorecognition-based control of attachment/detachment of MCF-7 cancer cells from polymer-coated surfaces is demonstrated. A glass surface is coated with a thermoresponsive statistical copolymer of poly(N-isopropylacrylamide-co-acrylamide) [p(NIPAm-co-Am)], which is end-capped with the Gly-Arg-Gly-Asp-Ser (GRGDS) p...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000277
更新日期:2020-11-04 00:00:00