Abstract:
:Injectable hydrogels are extensively used in drug delivery and tissue engineering to administer drugs, genes, growth factors and live cells. We report a method to produce tough, in-situ thermogelling, non-toxic, injectable hydrogels made of chitosan and hyaluronic acid co-crosslinked with β-glycerophophate and genipin. The gels are highly homogeneous and form within 32 min, i.e., faster than gels crosslinked with either genipin or β-glycerophophate. The shear strength of co-crosslinked hydrogels is 3.5 kPa, higher than any chitosan-based gel reported. Chondrocytes and nucleus pulposus cells thrive inside the gels and produce large amounts of collagen II. Injection in rats shows that the gels form in-vivo within a short time and remain well localized for more than one week while the rats remain healthy and active. The excellent mechanical properties, fast in-situ gelation, good biocompatibility and the ability to encapsulate live cells at physiological conditions make these hydrogels ideal for tissue engineering, especially cartilage regeneration.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Jalani G,Rosenzweig DH,Makhoul G,Abdalla S,Cecere R,Vetrone F,Haglund L,Cerruti Mdoi
10.1002/mabi.201400406subject
Has Abstractpub_date
2015-04-01 00:00:00pages
473-80issue
4eissn
1616-5187issn
1616-5195journal_volume
15pub_type
杂志文章abstract::Molecularly imprinted polymers (MIPs) are synthetic affinity matrices capable of selective binding a specific target molecule. A strategy for competitive selectivity studies is developed providing information on the selective binding properties of MIPs in complex matrices. Batch rebinding experiments entail the target...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201500106
更新日期:2015-11-01 00:00:00
abstract::In drug delivery to the human brain, blood vessels are a significant hurdle because they restrict the entry of most solutes to protect brain. To overcome this hurdle, an in vitro 3D model for brain endothelial barrier is developed using a microfluidic device with hydrogel providing a 3D extracellular matrix scaffold. ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201900425
更新日期:2020-06-01 00:00:00
abstract::The enzymatic transformation into an oligomer was carried out with the objective of developing the chemical recycling of bacterial polyesters. Poly(R-3-hydroxyalkanoate)s (PHAs), such as poly[(R-3-hydroxybutyrate)-co-12%(R-3-hydroxyhexanoate)] and poly[(R-3-hydroxybutyrate)-co-12%(R-3-hydroxyvalerate)], were degraded ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200500030
更新日期:2005-07-14 00:00:00
abstract::S-Layer proteins are an example of bionanostructures that can be exploited in nanofabrication. In addition to their ordered structure, the ability to self-assembly is a key feature that makes them a promising technological tool. Here, in vitro self-assembly kinetics of SpbA was investigated, and found that it occurs a...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200900175
更新日期:2010-02-11 00:00:00
abstract::Thermosensitive polymers enable externally controllable biomolecular interactions but hysteresis effects hamper the reversibility and repeated use of these materials. To quantify the temperature-dependent interactions and hysteresis effects, an optical adhesion assay based on poly(ethylene glycol) microgels (soft coll...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000186
更新日期:2020-10-01 00:00:00
abstract::Desferrioxamine (DFO) is a clinically approved, high affinity iron chelator used for the treatment of iron overload. Due to its short half-life and toxicity, DFO is administered for 8-12 h per day, 5-7 d per week. In this manuscript, the influence of molecular properties of hyperbranched polyglycerol (HPG)-DFO conjuga...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201600244
更新日期:2017-03-01 00:00:00
abstract::Novel block copolymers using the monomers 5-(2-methoxyethyoxymethyl)-5-methyl-[1,3]-dioxa-2-one (TMCM-MOE1OM) as a hydrophilic segment and lactides as a hydrophobic segment were designed in order to prepare controllable degradation polymers by dynamic polymer rearrangement based on the hydrophilicity. When the copolym...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200143
更新日期:2012-10-01 00:00:00
abstract::In the last decade, substantial research in the field of post-plasma grafting surface modification has focussed on the introduction of carboxylic acids on surfaces by grafting acrylic acid (AAc). In the present work, we report on an alternative approach for biomaterial surface functionalisation. Thin poly-ε-caprolacto...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000147
更新日期:2010-12-08 00:00:00
abstract::Cardiovascular diseases, including myocardial infarction, are the cause of significant morbidity and mortality globally. Tissue engineering is a key emerging treatment method for supporting and repairing the cardiac scar tissue caused by myocardial infarction. Creating cell supportive scaffolds that can be directly im...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201600446
更新日期:2017-07-01 00:00:00
abstract::The present study describes the synthesis of novel polypeptides containing O-phospho-L-threonine [Thr(PO(3)H(2))] and L-aspartic acid. Random copolypeptides copoly[Thr(PO(3)H(2))(X)Asp(Y)] (X:Y = 25:75, 50:50, 75:25), were conventionally prepared by copolymerization of Thr(PO(3)Ph(2)) N-carboxyanhydride (NCA) and Asp(...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200500211
更新日期:2006-03-14 00:00:00
abstract::The preparation of a novel peptide/dendrimer hybrid is reported in which an elastin-like oligopeptide is successfully assembled onto a poly(amidoamine) dendrimer surface (G4-ELP), and its unique thermo-responsive behavior is discussed. As a result, the G4-ELP is found to exhibit LCST behavior in the pH range 3-10 incl...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201100509
更新日期:2012-08-01 00:00:00
abstract::In-depth understanding of the biophysicochemical interactions at the nano-bio interface is important for basic cell biology and applications in nanomedicine and nanobiosensors. Here, the extracellular surface potential and topography changes of live cell membranes interacting with polymeric nanomaterials using a scann...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201800271
更新日期:2019-02-01 00:00:00
abstract::The antiviral effects of the cationic acrylate polymer E100 on a panel of lipid-enveloped viruses and the interactions involved are studied. The treatment of several common viruses with E100 induced a dose-dependent inhibition of the infectivity of viruses below the detection limit of the assays employed. Similarly, t...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200700102
更新日期:2007-09-11 00:00:00
abstract::Amino acid ester substituted polyphosphazenes are osteoactive benefiting from their phosphorus-containing chemical structure, which highlights interests in bone tissue engineering. To correlate their chemical structures with cell activities, in this study, poly[(ethyl alanato)0.3 (ethyl glycinato)0.7 phosphazene] (PAG...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201800464
更新日期:2019-06-01 00:00:00
abstract::A hybrid system for producing conducting polymers within a doping hydrogel mesh is presented. These conductive hydrogels demonstrate comparable electroactivity to conventional conducting polymers without requiring the need for mobile doping ions which are typically used in literature. These hybrids have superior mecha...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201100490
更新日期:2012-04-01 00:00:00
abstract::Open-pore biodegradable foams with controlled porous architectures were prepared by combining gas foaming and microparticulate templating. Microparticulate composites of poly(epsilon-caprolactone) (PCL) and micrometric sodium chloride particles (NaCl), in concentrations ranging from 70/30 to 20/80 wt.-% of PCL/NaCl we...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200700278
更新日期:2008-07-07 00:00:00
abstract::A nanoformulation composed of curdlan, a linear polysaccharide of 1,3-β-linked d-glucose units, hydrogen bonded to poly(γ -glutamic acid) (PGA), was developed to stimulate macrophage. Curdlan/PGA nanoparticles (C-NP) are formulated by physically blending curdlan (0.2 mg mL-1 in 0.4 m NaOH) with PGA (0.8 mg mL-1 ). For...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000358
更新日期:2021-01-01 00:00:00
abstract::Injectable delivery systems have been widely used in tissue engineering as they can deliver cells into the body in a minimally invasive manner. In this study, it is hypothesized that microspheres with a similar size of cells could effectively form a shear reversible aggregate in the presence of cells and the aggregate...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201300365
更新日期:2014-05-01 00:00:00
abstract::An antioxidant microgel with both glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities is reported. Using computational design and genetic engineering methods, the main catalytic components of GPx are fabricated onto the surface of ferritin. The resulting seleno-ferritin (Se-Fn) monomers can self-ass...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201300019
更新日期:2013-06-01 00:00:00
abstract::Bioinks play a key role in determining the capability of the biofabricatoin processes and the resolution of the printed constructs. Excellent biocompatibility, tunable physical properties, and ease of chemical or biological modifications of gelatin methacryloyl (GelMA) have made it an attractive choice as bioinks for ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000317
更新日期:2021-01-01 00:00:00
abstract::Comb-shaped glycopolymer/peptide bioconjugates are constructed by grafting reduced glutathione (GSH) onto acrylate-functional block glycocopolymers via thiol-ene click chemistry. In aqueous solution, the glycopolymer/GSH bioconjugate self-assembles to sugar-installed spherical micelles. The size of micelles decreases ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200274
更新日期:2012-11-01 00:00:00
abstract::Multicellular tumor spheroid (MCTS) mimics microenvironment for tumor formation and provides predictive insight for in vivo tests. The hanging drop (HD) method of spheroid generation is cost effective, but it is limited by a long time duration for spheroid development and a low rate of formation of larger spheroids. T...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000180
更新日期:2020-08-01 00:00:00
abstract::Primary human megakaryocytes, the precursor cells of platelets, are difficult to collect and cultivate. Polymers that enrich these cells without affecting their regulation or function are of interest for basic research as well as for cord blood transplantation purposes since co-transplantation of enriched megakaryocyt...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200368
更新日期:2013-04-01 00:00:00
abstract::The immobilization of enzymes into polymer hydrogels is a versatile approach to improve their stability and utility in biotechnological and biomedical applications. However, these systems typically show limited enzyme activity, due to unfavorable pore dimensions and low enzyme accessibility. Here, 3D jet writing of wa...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000154
更新日期:2020-09-01 00:00:00
abstract::The aggregative behaviors of hydroxypropylcellulose (HPC) molecules in aqueous solution and on substrates have been observed by employing laser light scattering (LLS) and, after deposition on a mica surface, atomic force microscopy (AFM). LLS studies showed that the HPC molecules formed large aggregates through self-a...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200600067
更新日期:2006-07-14 00:00:00
abstract::Hydroxypropyl chitosan-graft-carboxymethyl beta-cyclodextrin (HPCH-g-CM beta-CD) was synthesized by grafting CM beta-CD onto HPCH using water soluble 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) as the condensing agent. Due to the presence of hydrophobic beta-CD rings onto the HPCH backbone, this polymer can be...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200500087
更新日期:2005-10-20 00:00:00
abstract::Cell-free approaches to in situ tissue engineering require materials that are mechanically stable and are able to control cell-adhesive behavior upon implantation. Here, the development of mechanically stable grafts with non-cell adhesive properties via a mix-and-match approach using ureido-pyrimidinone (UPy)-modified...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201500278
更新日期:2016-03-01 00:00:00
abstract::In the present study, a pH responsive dendritic polyglycerol nanogel (dPG-NG) is developed to measure the pH values inside the hair follicle (HF) using an ex vivo porcine ear model. The macromolecular precursors are labeled with a pH sensitive indodicarbocyanine dye (pH-IDCC) and a control dye (indocarbocyanine dye: I...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201600505
更新日期:2017-10-01 00:00:00
abstract::An insoluble wheat proteins-based polymer networks with new functional groups and positive charges were prepared through grafting-coupling reactions. The networks were also flexible with a lower glass transition temperature since the existence of the grafted mobile segments. The multi-component/multi-phase systems bec...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200800156
更新日期:2009-01-09 00:00:00
abstract::Amylose selectively includes poly(L-lactide) (PLLA) among the poly(lactide)s (PLAs) to produce an inclusion complex when the phosphorylase-catalyzed polymerization of α-D-glucose 1-phosphate is performed in the presence of PLLA, poly(D-lactide) (PDLA), or poly(DL-lactide) (PDLLA) (vine-twining polymerization). This re...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201100133
更新日期:2011-10-10 00:00:00