CHO cells adhering to nitrogen-rich plasma-polymerised ethylene exhibit high production of a specific recombinant protein.

Abstract:

:In many industrial applications, inadequate cell attachment can be a limitation, especially when serum-free media are used. Nitrogen-rich plasma-polymerised ethylene (PPE:N) exhibits high concentrations of polar groups that can help to promote the attachment of weakly adherent cell types. Tissue plasminogen activator-producing Chinese hamster ovary (CHO) cells, adapted to suspension, were grown in the presence PPE:N flakes and were found to adhere to them. The growth rate was reduced, but cell viability was enhanced and their metabolism was more efficient, with generally higher recombinant protein productivity. Finally, cell adhesion on PPE:N surfaces was found to be independent of integrins, and was probably mediated by certain non-specific interactions with the PPE:N surface.

journal_name

Macromol Biosci

authors

Gigout A,Levasseur S,Girard-Lauriault PL,Buschmann MD,Wertheimer MR,Jolicoeur M

doi

10.1002/mabi.200900079

subject

Has Abstract

pub_date

2009-10-08 00:00:00

pages

979-88

issue

10

eissn

1616-5187

issn

1616-5195

journal_volume

9

pub_type

杂志文章
  • Ultrasound/Optical Dual-Modality Imaging for Evaluation of Vulnerable Atherosclerotic Plaques with Osteopontin Targeted Nanoparticles.

    abstract::Because of the high mortality of coronary atherosclerotic heart diseases, it is necessary to develop novel early detection methods for vulnerable atherosclerotic plaques. Phenotype transformation of vascular smooth muscle cells (VSMCs) plays a vital role in progressed atherosclerotic plaques. Osteopontin (OPN) is one ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201900279

    authors: Li S,Gou T,Wang Q,Chen M,Chen Z,Xu M,Wang Y,Han D,Cao R,Liu J,Liang P,Dai Z,Cao F

    更新日期:2020-02-01 00:00:00

  • Cell-Laden Hydrogels for Multikingdom 3D Printing.

    abstract::Living materials are created through the embedding of live, whole cells into a matrix that can house and sustain the viability of the encapsulated cells. Through the immobilization of these cells, their bioactivity can be harnessed for applications such as bioreactors for the production of high-value chemicals. While ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.202000121

    authors: Johnston TG,Fillman JP,Priks H,Butelmann T,Tamm T,Kumar R,Lahtvee PJ,Nelson A

    更新日期:2020-08-01 00:00:00

  • Sweet Vector for Gene Delivery: the Sugar Decoration of Polyplexes Reduces Cytotoxicity with a Balanced Effect on Gene Expression.

    abstract::The use of sugar-functionalized polyplexes as a nonviral gene delivery vector with lower cytotoxicity than the well-known polymeric carrier branched polyethyleneimine (BPEI) is investigated. The substitution of primary amine groups in the BPEI chains with lactose residues leads to larger polyplexes, presumably due to ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201700299

    authors: Albuquerque LJC,Alavarse AC,Carlan da Silva MC,Zilse MS,Barth MT,Bellettini IC,Giacomelli FC

    更新日期:2018-02-01 00:00:00

  • Curcumin Targeted, Polymalic Acid-Based MRI Contrast Agent for the Detection of Aβ Plaques in Alzheimer's Disease.

    abstract::Currently, there is no gadolinium-based contrast agent available for conventional magnetic resonance imaging (MRI) detection of amyloidal beta (Aβ) plaques in Alzheimer's disease (AD). Its timely finding would be vital for patient survival and quality of life. Curcumin (CUR), a common Indian spice effectively binds to...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201500062

    authors: Patil R,Gangalum PR,Wagner S,Portilla-Arias J,Ding H,Rekechenetskiy A,Konda B,Inoue S,Black KL,Ljubimova JY,Holler E

    更新日期:2015-09-01 00:00:00

  • Poly(ethylene glycol) grafting to poly(ether imide) membranes: influence on protein adsorption and thrombocyte adhesion.

    abstract::The chain length and end groups of linear PEG grafted on smooth surfaces is known to influence protein adsorption and thrombocyte adhesion. Here, it is explored whether established structure function relationships can be transferred to application relevant, rough surfaces. Functionalization of poly(ether imide) (PEI) ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201300309

    authors: Neffe AT,von Ruesten-Lange M,Braune S,Luetzow K,Roch T,Richau K,Jung F,Lendlein A

    更新日期:2013-12-01 00:00:00

  • Autoinducer Sensing Microarrays by Reporter Bacteria Encapsulated in Hybrid Supramolecular-Polysaccharide Hydrogels.

    abstract::A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported. For reinforcing ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201700176

    authors: Li P,Dou X,Müller M,Feng C,Chang MW,Frettlöh M,Schönherr H

    更新日期:2017-11-01 00:00:00

  • Conductive hydrogels: mechanically robust hybrids for use as biomaterials.

    abstract::A hybrid system for producing conducting polymers within a doping hydrogel mesh is presented. These conductive hydrogels demonstrate comparable electroactivity to conventional conducting polymers without requiring the need for mobile doping ions which are typically used in literature. These hybrids have superior mecha...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201100490

    authors: Green RA,Hassarati RT,Goding JA,Baek S,Lovell NH,Martens PJ,Poole-Warren LA

    更新日期:2012-04-01 00:00:00

  • Mitogenic activity of sulfated chitosan and cellulose derivatives is related to protection of FGF-2 from proteolytic cleavage.

    abstract::Novel chitosan (CHS) and cellulose sulfates (CSs) are studied regarding their mitogenic activity and their protective effect against proteolytic digestion of FGF-2. An intermediate degree of sulfation (DS(S) ) and lower concentration of CHS have superior effect on 3T3 cell growth while the mitogenic activity of CS inc...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201100518

    authors: Weltrowski A,da Silva Almeida ML,Peschel D,Zhang K,Fischer S,Groth T

    更新日期:2012-06-01 00:00:00

  • Characterizing biodegradation of PLA and PLA-g-AA/starch films using a phosphate-solubilizing bacillus species.

    abstract::The use of PLA and PLA-g-AA/starch as materials for the controlled release of encapsulated PSB was evaluated. The results showed that the bacterium degraded both the PLA and the PLA-g-AA/starch composite films, resulting in cell release. Severe disruption of the film structure occurred after incubation for 60-90 d. Th...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200700181

    authors: Wu CS

    更新日期:2008-06-11 00:00:00

  • Long-Term Stable Poly(acrylamide) Brush Modified Transparent Microwells for Cell Attachment Studies in 3D.

    abstract::The fabrication of 3D cell microenvironments exploiting versatile, long-term stable passivating poly(acryl amide) brushes in a microwell format and the study of the behavior of fibroblast and pancreatic tumor cells in wells of systematically varied shape and size is reported. The microwells, which are obtained by comb...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201600451

    authors: Lilge I,Jiang S,Schönherr H

    更新日期:2017-05-01 00:00:00

  • A microfluidic approach to encapsulate living cells in uniform alginate hydrogel microparticles.

    abstract::A microfluidic technique is described to encapsulate living cells in alginate hydrogel microparticles generated from monodisperse double-emulsion templates. A microcapillary device is used to fabricate double emulsion templates composed of an alginate drop surrounded by a mineral oil shell. Hydrogel formation begins w...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201100351

    authors: Martinez CJ,Kim JW,Ye C,Ortiz I,Rowat AC,Marquez M,Weitz D

    更新日期:2012-07-01 00:00:00

  • Self-assembling viral mimetics: one long journey with short steps.

    abstract::Recently, the Foresight Institute has pronounced six economic challenges that can be addressed through the progress of nanotechnology. One of these is the health and longevity of human life. Amongst applications anticipated to provide a solution to this challenge, gene therapy appears to be particularly promising. In ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章,评审

    doi:10.1002/mabi.201000330

    authors: Lamarre B,Ryadnov MG

    更新日期:2011-04-08 00:00:00

  • Amphiphilic Polymethyloxazoline-Polyethyleneimine Copolymers: Interaction with Lipid Bilayer and Antibacterial Properties.

    abstract::Polycations, mimicking activity of antibacterial peptides, belong to an important class of molecules investigated as a support or as an alternative to antibiotics. In this work, studies of modified linear amphiphilic statistical polymethyloxazoline (PMOX) and polyethyleneimine copolymers (PMOX_PEI) series are presente...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201900254

    authors: Kozon D,Mierzejewska J,Kobiela T,Grochowska A,Dudnyk K,Głogowska A,Sobiepanek A,Kuźmińska A,Ciach T,Augustynowicz-Kopeć E,Jańczewski D

    更新日期:2019-12-01 00:00:00

  • Multifunctional poly(Vinyl Amine)s bearing Azetidinium groups: one pot preparation in water and antimicrobial properties.

    abstract::A simple, robust one pot procedure for the preparation of waterborne multifunctional poly(vinyl amine)s (PVAms) is presented. By post-polymerization modification of PVAm with a bifunctional coupler and functional couplers cationic, reactive azetidinium groups, and alkyl chains are introduced in the side chains of PVAm...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201300576

    authors: Chattopadhyay S,Heine ET,Keul H,Möller M

    更新日期:2014-08-01 00:00:00

  • Surface modification of PLLA nano-scaffolds with laminin multilayer by LbL assembly for enhancing neurite outgrowth.

    abstract::In this study, PLLA nanofibers are fabricated by electrospinning and their surfaces are modified by laminin/chitosan (LN/CS) polyelectrolyte multilayer. Surface C/N ratio determined by XPS analysis quantitatively indicates of discrete coating layers on the nanofibers. The amount of LN deposited sustainably increases w...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201300177

    authors: He L,Tang S,Prabhakaran MP,Liao S,Tian L,Zhang Y,Xue W,Ramakrishna S

    更新日期:2013-11-01 00:00:00

  • Nanofiber formation of hydroxylpropylcellulose (HPC).

    abstract::The aggregative behaviors of hydroxypropylcellulose (HPC) molecules in aqueous solution and on substrates have been observed by employing laser light scattering (LLS) and, after deposition on a mica surface, atomic force microscopy (AFM). LLS studies showed that the HPC molecules formed large aggregates through self-a...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200600067

    authors: Yan L,Lin W,Bangal PR

    更新日期:2006-07-14 00:00:00

  • Hydroxypropyl chitosan bearing beta-cyclodextrin cavities: synthesis and slow release of its inclusion complex with a model hydrophobic drug.

    abstract::Hydroxypropyl chitosan-graft-carboxymethyl beta-cyclodextrin (HPCH-g-CM beta-CD) was synthesized by grafting CM beta-CD onto HPCH using water soluble 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) as the condensing agent. Due to the presence of hydrophobic beta-CD rings onto the HPCH backbone, this polymer can be...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200500087

    authors: Prabaharan M,Mano JF

    更新日期:2005-10-20 00:00:00

  • Microparticles for drug delivery based on functional polycaprolactones with enhanced degradability: loading of hydrophilic and hydrophobic active compounds.

    abstract::Microparticle drug carriers made of biodegradable functional polyesters were produced. The polyesters consist of a poly(ε-caprolactone) backbone bearing pendant acryloyloxy and methacryloyloxy groups. Stable microparticles were prepared via an oil/water emulsion-solvent evaporation technique eventually combined with a...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201000023

    authors: Vaida C,Mela P,Kunna K,Sternberg K,Keul H,Möller M

    更新日期:2010-08-11 00:00:00

  • Synthesis of oligosaccharide-branched ribofuranan by ring-opening polymerization of a new anhydroribo trisaccharide monomer.

    abstract::A new anhydroribotrisaccharide monomer, A2B3LR (1), was synthesized and ROP was carried out to elucidate the polymerizability and to obtain oligosaccharide-branched polysaccharides with defined structures. The new trisaccharide monomer was found to be polymerized readily with BF(3) . OEt(2) as a catalyst at -40 degree...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200800316

    authors: Yoshida D,Han S,Narita K,Hattori K,Yoshida T

    更新日期:2009-07-07 00:00:00

  • Development of a Dual Hydrogel Model System for Vascularization.

    abstract::Numerous hydrogel-based culture systems are used to create in vitro model for prevascularization. Hydrogels used to induce a microenvironment conducive to microvessel formation are typically soft and fast degradable, but often suffer from maintaining a lasting perfusable channel in vitro. Here, a dual hydrogel system ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.202000204

    authors: Kim S,Pan CC,Yang YP

    更新日期:2020-10-01 00:00:00

  • A biodegradable polymersome with pH-tuning on-off membrane based on poly(β-amino ester) for drug delivery.

    abstract::A nanoscale biodegradable polymersome with pH-tuning on-off membrane is prepared via the self-assembly of poly(β-amino ester)-based amphiphilic copolymers. The pH-sensitive polymersome-like vesicle structure includes two layers that can encapsulate either hydrophobic or hydrophilic therapeutic drugs at physiological p...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201200468

    authors: Jeong IK,Gao GH,Li Y,Kang SW,Lee DS

    更新日期:2013-07-01 00:00:00

  • O-Mannosylation Affords a Glycopeptide Hydrogel with Inherent Antibacterial Activities against E. coli via Multivalent Interactions between Lectins and Supramolecular Assemblies.

    abstract::Multivalent carbohydrate-lectin interactions play a crucial role in bacterial infection. Biomimicry of multivalent glycosystems represents a major strategy in the repression of bacterial growth. In this study, a new kind of glycopeptide (Naphthyl-Phe-Phe-Ser-Tyr, NMY) scaffold with mannose modification is designed and...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201900124

    authors: Li J,Liang S,Yan Y,Tian X,Li X

    更新日期:2019-09-01 00:00:00

  • Development and Characterization of Ultrasound Activated Lipopolyplexes for Enhanced Transfection by Low Frequency Ultrasound in In Vitro Tumor Model.

    abstract::This work focuses on the development of ultrasound contrast vesicles for ultrasound-mediated enhanced transfection of nucleic acids in the cancer cells and projects its application as a tool for diagnostic imaging. The ultrasound contrast vesicles are stable, anionic, nanoscaled vesicles with ultrasound contrast equiv...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.202000173

    authors: Shah H,Tariq I,Engelhardt K,Bakowsky U,Pinnapireddy SR

    更新日期:2020-12-01 00:00:00

  • pH-Triggered Self-Assembly of Cellulose Oligomers with Gelatin into a Double-Network Hydrogel.

    abstract::Multicomponent systems for self-assembled molecular gels provide huge opportunities to generate collective or new functions that are not inherent in individual single-component gels. However, gelation tends to require careful and complicated procedures, because, among a myriad of kinetically trapped structures related...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.202000187

    authors: Hata Y,Kojima T,Maeda T,Sawada T,Serizawa T

    更新日期:2020-09-01 00:00:00

  • Amides and Heparin-Like Polymer Co-Functionalized Graphene Oxide Based Core @ Polyethersulfone Based Shell Beads for Bilirubin Adsorption.

    abstract::Excessive bilirubin in the body of patient with liver dysfunction or metabolic obstruction may cause jaundice with irreversible brain damage, and new type of adsorbent for bilirubin is under frequent investigation. Herein, graphene oxide based core @ polyethersulfone-based shell beads are fabricated by phase inversion...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.202000153

    authors: He C,Li M,Zhang J,Yan B,Zhao W,Sun S,Zhao C

    更新日期:2020-08-01 00:00:00

  • Synthesis and Validation of Functional Nanogels as pH-Sensors in the Hair Follicle.

    abstract::In the present study, a pH responsive dendritic polyglycerol nanogel (dPG-NG) is developed to measure the pH values inside the hair follicle (HF) using an ex vivo porcine ear model. The macromolecular precursors are labeled with a pH sensitive indodicarbocyanine dye (pH-IDCC) and a control dye (indocarbocyanine dye: I...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201600505

    authors: Dimde M,Sahle FF,Wycisk V,Steinhilber D,Camacho LC,Licha K,Lademann J,Haag R

    更新日期:2017-10-01 00:00:00

  • Open-pore biodegradable foams prepared via gas foaming and microparticulate templating.

    abstract::Open-pore biodegradable foams with controlled porous architectures were prepared by combining gas foaming and microparticulate templating. Microparticulate composites of poly(epsilon-caprolactone) (PCL) and micrometric sodium chloride particles (NaCl), in concentrations ranging from 70/30 to 20/80 wt.-% of PCL/NaCl we...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200700278

    authors: Salerno A,Iannace S,Netti PA

    更新日期:2008-07-07 00:00:00

  • Multiphysics modelling of volume phase transition of ionic hydrogels responsive to thermal stimulus.

    abstract::This paper presents the analysis of the volume phase transition of ionic thermo-sensitive hydrogels to thermal stimulus through mathematical modelling. The model is termed the multi-effect-coupling thermal-stimulus (MECtherm) model and it considers the effects of multi-phases and multi-physics. Its application to stea...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200500082

    authors: Li H,Wang X,Wang Z,Lam KY

    更新日期:2005-09-16 00:00:00

  • Selective Cell Adhesion and Biosensing Applications of Bio-Active Block Copolymers Prepared by CuAAC/Thiol-ene Double Click Reactions.

    abstract::N-Acetyl-l-cysteine (NAC)-capped poly(methyl methacrylate)-b-polycaprolactone block copolymer (PMMA-b-PCL-NAC) was prepared using the previously described one-pot photoinduced sequential CuAAC/thiol-ene double click procedure. PMMA-b-PCL-NAC had previously shown good applicability as a matrix for cell adhesion of cell...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201500099

    authors: Oyman Eyrilmez G,Doran S,Murtezi E,Demir B,Odaci Demirkol D,Coskunol H,Timur S,Yagci Y

    更新日期:2015-09-01 00:00:00

  • Butyrate-Loaded Chitosan/Hyaluronan Nanoparticles: A Suitable Tool for Sustained Inhibition of ROS Release by Activated Neutrophils.

    abstract::Tissue damage caused by excessive amounts of neutrophil-derived reactive oxygen species (ROS) occurs in many inflammatory diseases. Butyrate is a short-chain fatty acid (SCFA) with known anti-inflammatory properties, able to modulate several neutrophil functions. Evidence is provided here that butyrate inhibits neutro...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201700214

    authors: Sacco P,Decleva E,Tentor F,Menegazzi R,Borgogna M,Paoletti S,Kristiansen KA,Vårum KM,Marsich E

    更新日期:2017-11-01 00:00:00