Abstract:
:Tissue-dependent oestrogenic and anti-oestrogenic activity of polycyclic aromatic hydrocarbons (PAHs) has been suggested. In this study, the effect of two PAH mixtures, M1 composed of all 16 priority pollutants and M2 composed of five (noted in the highest levels) compounds, on follicle-stimulating hormone receptor (FSHR) expression, basal or FSH-induced oestradiol (E2) secretion and aromatase cytochrome P450 (P450arom) protein expression, by non-luteinised human granulosa cell line (HGrC1) was determined. In addition, the consequences of gene silencing of oestrogen receptor alfa (siESR1), oestrogen receptor beta (siESR2) and a G protein-coupled receptor (siGPER1) on the above parameters were described. Neither PAH mixture had an effect on basal FSHR protein expression; however, both mixtures increased FSH-induced FSHR expression. Decreased E2 secretion and P450arom expression was also demonstrated. In both basal and FSH treated cells, siESR1 and siGPER1 reversed the inhibitory effect of the mixtures on E2 secretion; however, in siESR2 cells, the inhibitory effect was still observed. This study showed that both classic ESR1 and GPER1 were involved in the inhibitory effect of both PAH mixtures on E2 secretion and confirmed that expression of P450arom could be downregulated through the aryl hydrocarbon receptor and additionally through the ESR2.
journal_name
Hum Exp Toxicoljournal_title
Human & experimental toxicologyauthors
Zajda K,Gregoraszczuk ELdoi
10.1177/0960327119886027subject
Has Abstractpub_date
2020-03-01 00:00:00pages
276-289issue
3eissn
0960-3271issn
1477-0903journal_volume
39pub_type
杂志文章abstract::The pharmacokinetics of the herbicide fluazifop-butyl have been determined in female rats following oral and intravenous dosing, and described by a mathematical model. Penetration of fluazifop-butyl through epidermal membranes has been determined using three different receptor fluids. It is demonstrated how this in vi...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719301200302
更新日期:1993-05-01 00:00:00
abstract::Exposure of Co631 cells to plutonium leads to increasing cell death depending on the dose and incubation time. The effects, however, are more pronounced with 239Pu, than with 238Pu. Uptake and binding of the two radionuclides during a 5-h incubation are about equal, but 238Pu is lost more rapidly from the cell. There ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719201100303
更新日期:1992-05-01 00:00:00
abstract::Formaldehyde is a physiological intermediary metabolite taking part in many biological process in the body. It is a constituent of many items of daily use, including foods. It is also used in medicine for treatment of some conditions. A 40% solution of formaldehyde in water is known as formalin. Formalin is irritating...
journal_title:Human & experimental toxicology
pub_type: 杂志文章,评审
doi:10.1191/096032700678815954
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abstract::1 The cytotoxicity of dichlorobenzenes in cultured rat liver slices has previously been shown to be strain specific and biotransformation related. 2 In order to extrapolate animal models to humans, the dichlorobenzenes were incubated with human liver slices to try to clarify their hepatotoxic potential in man. 3 The d...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719101000510
更新日期:1991-09-01 00:00:00
abstract::1. 26Al and 67Ga were given as citrates to a healthy male volunteer by intravenous injection. The retention of both tracers was studied by body radioactivity measurement. Levels in blood and excreta were determined by gamma-ray spectrometry and/or accelerator mass spectrometry. 2. More than half of the 26Al had left t...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719501400309
更新日期:1995-03-01 00:00:00
abstract::This study was focused on investigating the anticancer potential of indole-3-carbinol (I3C) against lung cancer H1299 cells via an increase in ROS levels. To investigate the induction of growth arrest and/or cell death in H1299 cells, a cell cycle arrest assay, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327120969968
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abstract::Diabetes mellitus promoted an overproduction of free radicals and an increased incidence of both diabetic nephropathy and liver disease. In this report, we evaluated the effects of Chinese and Brazilian propolis on streptozotocin-induced hepatorenal injury in rats. The results demonstrated that Chinese propolis-treate...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327110387456
更新日期:2011-09-01 00:00:00
abstract::Epichlorohydrin (ECH) is an antifertility agent that acts both as an epididymal toxicant and an agent capable of directly affecting sperm motility. This study identified the time course of apoptotic cell death in rat epididymides after ECH treatment. Rats were administrated with a single oral dose of ECH (50 mg/kg). E...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327112467042
更新日期:2013-06-01 00:00:00
abstract::1. In humans morphine is metabolised to morphine-3-glucuronide (M3G) which possess no opioid activity, and morphine-6-glucuronide (M6G) which is a potent opioid receptor agonist that probably contribute to the desired as well as toxic effects of morphine. 2. In order to investigate the possible effect of ranitidine on...
journal_title:Human & experimental toxicology
pub_type: 临床试验,杂志文章
doi:10.1177/096032719801700611
更新日期:1998-06-01 00:00:00
abstract::The study was done to find out whether subacute exposure to supercypermethrin forte (SCM) affected cellular and humoral immunity. Groups of 10 male Wistar rats were given SCM by gavage for 28 days at 12.5 mg kg-1 day-1 (1/14 LD50), 8.75 mg kg-1 day-1 (1/20 LD50) and 4.38 mg kg-1 day-1 (1/40 LD50) and the response of s...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719501400502
更新日期:1995-05-01 00:00:00
abstract::Sulfur dioxide (SO2) is a common exogenous atmospheric pollutant. Studies have shown that SO2 can cause vasodilation as a gas signaling molecule, but the specific signaling pathways are not well understood. This study aimed to explore the underlying mechanism behind the effects of SO2 on vasodilation of isolated rat a...
journal_title:Human & experimental toxicology
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doi:10.1177/0960327120911428
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abstract::The principal impediment to gene therapy is the development of efficient, nontoxic gene carriers that can handle and deliver foreign genetic materials into various cell types, including healthy and cancerous cells. Poly-l-lysine (PLL) polymers are one of the most favorable gene carriers among nonviral vectors, and PLL...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327119846924
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abstract::Lead exposure was assessed in a random cohort of 70 male papier mâché workers and compared with 35 age and sex matched controls. Blood-lead levels in workers were significantly higher than in controls (Mean 68.07 +/- 44.64 ug/dl vs 25.52 +/- 16.56 ug/dl respectively; P < 0.001). Urinary lead was also higher in workers...
journal_title:Human & experimental toxicology
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doi:10.1177/096032719701600510
更新日期:1997-05-01 00:00:00
abstract::1. The coumarin anticoagulant difenacoum was detected by high performance liquid chromatography (HPLC) with multi-wavelength UV detection in plasma from a 41 years old man who presented with a severe deficiency of vitamin K-dependent clotting factors of unknown aetiology. A longitudinal toxicological study of the cons...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719701600306
更新日期:1997-03-01 00:00:00
abstract::1. Osmolal and anion gaps are helpful in the diagnosis and evaluation of intoxications with methanol and ethylene glycol. Reported reference values for osmolal gap and anion gap are -1 (+/- 6) mosm kg-1 H2O and 16 (+/- 2) mmol l-1, respectively. However, we have repeatedly found unexplained increased gaps in patients ...
journal_title:Human & experimental toxicology
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doi:10.1177/096032719401300212
更新日期:1994-02-01 00:00:00
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journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719301200216
更新日期:1993-03-01 00:00:00
abstract::A liquid alcoholic extract of Papaver somniferum named Elixir Paregorico is extensively used for diarrheal diseases in Brazil. Its increased popularity has brought concerns and fears over the safety of this herbal product. Given the lack of investigative clinical studies, in this regard, this study investigated whethe...
journal_title:Human & experimental toxicology
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doi:10.1177/0960327108090274
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abstract::1. Multiple low doses of the direct acting organophosphates, ecothiopate, paraoxon and mipafox produced persistent and additive inhibition of diaphragm acetylcholinesterase. Paraoxon and mipafox had similar effects on brain acetylcholinesterase. There was greater recovery from inhibition between doses for paraoxon and...
journal_title:Human & experimental toxicology
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journal_title:Human & experimental toxicology
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abstract::Gamma-hydroxybutyrate (GHB) is a compound used in the treatment of alcohol withdrawal, narcolepsy, and for induction of anaesthesia. It is also contained in many products illegally marketed as "dietary supplements" and is increasingly being recognised as a potential drug of abuse. We report the case of a 44-year-old m...
journal_title:Human & experimental toxicology
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journal_title:Human & experimental toxicology
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abstract::Exposure to quartz induces pulmonary inflammation and development of fibrosis. In order to study the fibrosing process, we investigated morphology, function and phenotype of alveolar (AMs) and interstitial (IMs) macrophages at an early stage of fibrosis in rats. Rats were exposed by intratracheal instillations of 10 m...
journal_title:Human & experimental toxicology
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abstract::1. A modified mouse liver slice culture technique was established and the viability of the system was assessed on the basis of leakage of cytosolic enzymes viz. lactate dehydrogenase (LDH), alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartic aminotransferase (AST) and slice histology. 2. This system w...
journal_title:Human & experimental toxicology
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doi:10.1177/096032719601500202
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abstract::Trichloroethylene (TCE) was exposed in the presence of the spin trap N-tert-butyl-alpha-phenyl nitrone (PBN, 0.1 M) to ionizing radiation from two different sources in an attempt to determine the origin of the spin-trapped radicals generating the EPR spectra in precision cut liver slices. TCE samples were irradiated w...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719701600607
更新日期:1997-06-01 00:00:00
abstract::This study deals with pesticide exposure profile in some European countries with a specific focus on ethylenebisdithiocarbamates (EBDC). In all, 55 Bulgarian greenhouse workers, 51 Finnish potato farmers, 48 Italian vineyard workers, 42 Dutch floriculture farmers, and 52 Bulgarian zineb producers entered the study. Ea...
journal_title:Human & experimental toxicology
pub_type: 杂志文章,多中心研究
doi:10.1177/0960327108100003
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journal_title:Human & experimental toxicology
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abstract::Sibutramine mesylate is a new anti-obesity drug. It is a crystalline salt of sibutramine developed to improve the solubility of sibutramine hydrochloride. Methanesulfonic acid was used as a salt-forming acid instead of hydrochloric acid, resulting in a greatly improved solubility of 1000 mg/mL in water. Sibutramine me...
journal_title:Human & experimental toxicology
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abstract::Severe carbon monoxide (CO) poisoning causes fulminant deaths in common environment as well as neurological sequelae to survivors. Prevention of delayed neurological syndrome (DNS) after exposure to CO, the most important sequela, is based up to date on hyperbaric oxygen administration. Nevertheless, its use remains c...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327120901572
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abstract::Methanol, a potent toxicant in humans, occurs naturally at a low level in most alcoholic beverages without causing harm. However, illicit drinks made from "industrial methylated spirits" [5% (v/v) methanol:95% (v/v) ethanol] can cause severe and even fatal illness. Since documentation of a no-adverse-effect level for ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1191/096032701718620864
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abstract::In the 1980s, villages in the Kurdistan region of Iraq were exposed to chemical weapons (CWs), which killed and injured thousands of civilians. There has been no clinical assessment of the long-term effects of CWs exposure on those injured. We report the first such evaluation of CW effects on long-term health of child...
journal_title:Human & experimental toxicology
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更新日期:2018-08-01 00:00:00