Abstract:
:This study was focused on investigating the anticancer potential of indole-3-carbinol (I3C) against lung cancer H1299 cells via an increase in ROS levels. To investigate the induction of growth arrest and/or cell death in H1299 cells, a cell cycle arrest assay, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) assay, and reactive oxygen species (ROS) detection assay were performed. Through the TUNEL assay, we detected I3C-induced DNA fragmentation. Fluorescence-activated cell sorting (FACS) analysis showed that I3C induced an increase in ROS levels and apoptotic rate in a dose- and time-dependent manner in H1299 cells. Western blotting demonstrated that activated forms of caspase-3, caspase-7, caspase-9, and poly (ADP-ribose) polymerase (PARP) were increased in I3C-treated H1299 cells following treatment with I3C. Furthermore, protein expression levels of FOXO3, bim, bax, and phosphorylated ERK and JNK were increased, while those of pAkt, Bcl-xL, and Bcl-2 were decreased by I3C treatment of H1299 cells. To confirm the relationship between cell apoptosis and ROS generation, H1299 cells were treated with I3C simultaneously with N-acetylcysteine (NAC), and it was shown that ROS levels decreased and viability increased. Moreover, in western blot analysis, expression of anti-apoptotic proteins (thioredoxin1, peroxiredoxin-1, Bcl-2, and Bcl-xL) in I3C-treated cells was evidently downregulated and pro-apoptotic proteins (active ASK1 and cleaved PARP) were upregulated compared to cells co-treated with NAC. The study showed that I3C induced downregulation of ROS regulator proteins and elevation of ROS, thus activating apoptotic signaling cascades in human lung cancer H1299 cells.
journal_name
Hum Exp Toxicoljournal_title
Human & experimental toxicologyauthors
Lim HM,Park SH,Nam MJdoi
10.1177/0960327120969968subject
Has Abstractpub_date
2020-10-29 00:00:00pages
960327120969968eissn
0960-3271issn
1477-0903pub_type
杂志文章abstract::The present study has three purposes; first evaluating cytotoxicity of (E)-4-chloro-2-((3-ethoxy-2-hydroxybenzylidene)amino)phenol (ACES), second deciphering ACES-mediated cellular death mechanism, and third estimating ACES-mediated alterations in the expressions of mitogen-activated protein kinase (MAPK) pathway-rela...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327120924112
更新日期:2020-10-01 00:00:00
abstract::The renin-angiotensin system (RAS) subserves vital physiological functions and also implicated in certain pathological states. Modulation of this system has been proposed in recent studies to be a promising strategy in treating liver fibrosis. We investigated the effect of the pharmacologic inhibition of RAS with angi...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327111401051
更新日期:2011-11-01 00:00:00
abstract:INTRODUCTION:Poisoning from organophosphates and carbamates is a significant cause of morbidity and mortality worldwide. Concerns have been expressed over the safety and efficacy of the use of oximes such as pralidoxime (2-PAM) in patients with carbamate poisoning in general, and more so with carbaryl poisoning specifi...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327107070849
更新日期:2007-02-01 00:00:00
abstract::Diabetes mellitus promoted an overproduction of free radicals and an increased incidence of both diabetic nephropathy and liver disease. In this report, we evaluated the effects of Chinese and Brazilian propolis on streptozotocin-induced hepatorenal injury in rats. The results demonstrated that Chinese propolis-treate...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327110387456
更新日期:2011-09-01 00:00:00
abstract::1. The effect of pyridostigmine on cardiorespiratory function after oxime + atropine injection was investigated in tabun poisoned guinea-pigs and without tabun poisoning. 2. The trachea, a carotid artery and jugular vein were cannulated in female urethane-anaesthetised Pirbright-white guinea-pigs. After baseline measu...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719501400803
更新日期:1995-08-01 00:00:00
abstract::The aim of this study was to evaluate the scores of the Acute Physiology and Chronic Health Evaluation (APACHE) II and a modified APACHE II system (MAS), without parameters of biochemical tests; and to find prognostic value of individual elements of the APACHE II and MAS in predicting outcomes in organophosphate (OP) ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/09603271060080076
更新日期:2007-07-01 00:00:00
abstract::Rosiglitazone is in the thiazolidinedione class of drugs used in the treatment of type 2 diabetes mellitus. It works as an insulin sensitizer by binding to the peroxisome proliferator-activated receptor gamma. We investigated the effects of prenatally administered rosiglitazone on pyramidal cell numbers and morphologi...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327117730883
更新日期:2018-08-01 00:00:00
abstract::1. Multiple low doses of the direct acting organophosphates, ecothiopate, paraoxon and mipafox produced persistent and additive inhibition of diaphragm acetylcholinesterase. Paraoxon and mipafox had similar effects on brain acetylcholinesterase. There was greater recovery from inhibition between doses for paraoxon and...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719701600201
更新日期:1997-02-01 00:00:00
abstract::Predictive toxicology plays a critical role in reducing the failure rate of new drugs in pharmaceutical research and development. Despite recent gains in our understanding of drug-induced toxicity, however, it is urgent that the utility and limitations of our current predictive tools be determined in order to identify...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327115605440
更新日期:2015-12-01 00:00:00
abstract::Dimethylacetamide (DMAc) is a skin-penetrating solvent able to induce hepatic damage after chronic exposure. Previous research has indicated that metabolism may be saturated at its present TLV/TWA (10 ppm). Biological monitoring of monomethylacetamide (MMAc), the primary metabolite of DMAc, might therefore underestima...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719301200206
更新日期:1993-03-01 00:00:00
abstract:BACKGROUND:Diphenhydramine (DPHM) overdose is a frequent cause of acute poisoning. Although its clinical features are well known, information about the dose-dependent toxicity of DPHM is still scarce. The objective of this study was to investigate the dose-dependent toxicity of DPHM in patients with acute DPHM poisonin...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1191/096032700671040438
更新日期:2000-09-01 00:00:00
abstract:AIM:To assess the systemic inflammatory response (SIR) and the multi-organ damage after large-volume liver radiofrequency ablation (RFA) with or without concurrent Pringle maneuver. METHODS:Wistar rats were subjected to 30% liver RFA (group RFA), liver RFA under 30-min Pringle maneuver (group RFA + P), Pringle only (g...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327111401438
更新日期:2011-11-01 00:00:00
abstract::1. Carbofuran was administered orally to adult male rats at dose levels of 0.1, 0.2, 0.4 or 0.8 mg kg-1 body weight, 5 d wk-1 for 60 days. A dose dependent decrease was observed in body weight of rats treated with 0.2-0.8 mg carbofuran kg-1 body weight. 2. A significant decrease in the weight of epididymides, seminal ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719501401106
更新日期:1995-11-01 00:00:00
abstract::Phosphorus flame retardants are a group of chemicals that are used to slow or prevent the spread of fire. These compounds have been detected in different environments including human organism. In the present study, we have investigated DNA-damaging potential and effect on DNA methylation of tris(2-chloroethyl) phospha...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327119839174
更新日期:2019-06-01 00:00:00
abstract::Okadaic acid (OA) is the main toxin produced by dinoflagellates, which can accumulate in the hepatopancreas of mussels and cause diarrhoetic shellfish poisoning in consumers. This toxin is also a tumour promoter and a specific potent inhibitor of protein phosphatases 1 and 2A. The results in this study show for the fi...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719601500307
更新日期:1996-03-01 00:00:00
abstract::1. This work examines the influence of age on some nephrotoxic signs of gentamicin in normal and diabetic rats (aged 1 and 24 months). 2. Gentamicin (80 mg-1 kg-1 day for 6 days, intramuscularly) produced the typical pattern of nephrotoxicity; significantly increasing the plasma concentrations of creatinine and urea, ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719601500109
更新日期:1996-01-01 00:00:00
abstract::Dimerized translationally controlled tumor protein (dTCTP) plays a role in allergic diseases. A 7-mer peptide, dimerized translationally binding protein 2 (dTBP2), binds to dTCTP and inhibits dTCTP, suggesting that the 7-mer peptide may have therapeutic potential. We assessed the safety of dTBP2 by examining its cytot...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327113477873
更新日期:2013-11-01 00:00:00
abstract::Persistent organochlorine pollutants (POPs), such as polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins (PCDDs), dichloro diphenyl trichloroethane (DDT) and its major metabolite 1,1-dichloro-2,2-bis (p-chlorophenyl)-ethylene (p,p' -DDE) have been associated with type 2 diabetes mellitus (T2DM) in rece...
journal_title:Human & experimental toxicology
pub_type: 杂志文章,多中心研究
doi:10.1177/0960327107076886
更新日期:2007-05-01 00:00:00
abstract::This study deals with pesticide exposure profile in some European countries with a specific focus on ethylenebisdithiocarbamates (EBDC). In all, 55 Bulgarian greenhouse workers, 51 Finnish potato farmers, 48 Italian vineyard workers, 42 Dutch floriculture farmers, and 52 Bulgarian zineb producers entered the study. Ea...
journal_title:Human & experimental toxicology
pub_type: 杂志文章,多中心研究
doi:10.1177/0960327108100003
更新日期:2008-09-01 00:00:00
abstract::1. In humans CaNa2EDTA increases urinary excretion of lead as well as that of essential metals such as zinc, ferrum and manganese but not that of copper. 2. We studied the effect of CaNa2EDTA injection on serum dopamine-beta-hydroxylase, a copper-dependent enzyme, in three male lead welders hospitalized for suspected ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719401300405
更新日期:1994-04-01 00:00:00
abstract::The malignancy of non-small cell lung cancer (NSCLC) largely results from its invasive manner. Secreted frizzled-related proteins (SFRPs) have been recently found to suppress the invasiveness of some cancers. On the other hand, the methylation of SFRPs increases protein degradation to reduce the activity of SFRPs, res...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327117693071
更新日期:2018-02-01 00:00:00
abstract:BACKGROUND:The mortality rate associated with human glufosinate poisoning is high. We evaluated the usefulness of serum ammonia and sequential organ failure assessment (SOFA) and acute physiology and chronic health evaluation II (APACHE II) scores for early prediction of in-hospital mortality in glufosinate ammonium po...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327119855124
更新日期:2019-09-01 00:00:00
abstract::Naringenin is a naturally occurring flavanones and has been found to exhibit free radical scavenging, enzyme inhibition, antioxidants, anti-inflammatory, and anticancer activities. Present study was designed to evaluate the protective role of naringenin against benzo[a]pyrene (B[a]P)-induced oxidative stress and pulmo...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327116650009
更新日期:2017-04-01 00:00:00
abstract::Comparisons on a linear and the Rozman logarithmic scale for dosage versus carcinogenicity in rodents are presented for methyl eugenol (ME), nitrosodiethylamine (NDEA), ethyl carbamate (EC) and 2-acetylaminofluorene (AAF). Each of these chemicals has been shown to be carcinogenic in experimental animals and, in additi...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1191/0960327105ht525oa
更新日期:2005-06-01 00:00:00
abstract::Enterococcus faecalis is a facultative anaerobic gram-positive commensal bacterium common in the gastrointestinal tract of animals and humans. This study aimed to investigate the protective effects of heat-killed E. faecalis EF-2001 (EF-2001) on acute gastric ulcer using a murine model of ethanol (EtOH)-induced acute ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327119899987
更新日期:2020-05-01 00:00:00
abstract::This study was designed to evaluate the maternal toxicity and teratogenicity of fenproporex, one of the most widely-used anorectic drugs in many countries, including Brazil. Three periods of exposure were evaluated: (a) 30 days before mating; (b) from gestational day (GD) 0 to 14; and (c) 30 days before mating and dur...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1191/0960327105ht545oa
更新日期:2005-08-01 00:00:00
abstract::This descriptive and retrospective study was conducted at the poisoning ward of Imam teaching hospital, Sari, Iran, with the aim of evaluating the pattern of poisoning. Hence, the medical profiles of 2057 patients, who were admitted, were carefully reviewed during the period from April 2006 to March 2008 for 2 years. ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327110361501
更新日期:2010-09-01 00:00:00
abstract::The US Environmental Protection Agency (EPA) recently issued a Staff Paper that articulates current risk assessment practices. In section 4.1.3, EPA states, "... effects that appear to be adaptive, non-adverse, or beneficial may not be mentioned." This statement may be perceived as precluding risk assessments based on...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1191/0960327106ht580oa
更新日期:2006-01-01 00:00:00
abstract::Progesterone (a neurosteroid) is an important modulator of the nervous system functioning. Organophosphorus pesticides like phosphamidon have been shown to adversely affect memory and induce oxidative stress on both acute and chronic exposure. The present study was therefore designed to investigate the effects of prog...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327110396522
更新日期:2011-10-01 00:00:00
abstract::The Organization for Economic Cooperation and Development (OECD) has promoted Good Laboratory Practice (GLP) since the mid 1970s. This paper describes the development of the concept through international harmonisation, Quality Assurance (QA), and compliance monitoring. Ultimately this process permits Mutual Acceptance...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719301200611
更新日期:1993-11-01 00:00:00