Enhanced photoinactivation of Staphylococcus aureus with nanocomposites containing plasmonic particles and hematoporphyrin.

Abstract:

:We fabricated composite nanoparticles consisting of a plasmonic core (gold nanorods or gold-silver nanocages) and a hematoporphyrin-doped silica shell. The dual photodynamic and photothermal activities of such nanoparticles against Staphylococcus aureus 209 P were studied and compared with the activities of reference solutions (hematoporphyrin or silica-coated plasmonic nanoparticles). Bacteria were incubated with nanocomposites or with the reference solutions for 15 min, which was followed by CW light irradiation with a few exposures of 5 to 30 min. To stimulate the photodynamic and photothermal activities of the nanocomposites, we used LEDs (405 and 625 nm) and a NIR laser (808 nm), respectively. We observed enhanced inactivation of S. aureus 209 P by nanocomposites in comparison with the reference solutions. By using fluorescence microscopy and spectroscopy, we explain the enhanced antimicrobial effect of hematoporphyrin-doped nanocomposites by their selective accumulation in the vicinity of the bacteria.

journal_name

J Biophotonics

journal_title

Journal of biophotonics

authors

Khlebtsov BN,Tuchina ES,Khanadeev VA,Panfilova EV,Petrov PO,Tuchin VV,Khlebtsov NG

doi

10.1002/jbio.201200079

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

338-51

issue

4

eissn

1864-063X

issn

1864-0648

journal_volume

6

pub_type

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