Role of blood oxygenation saturation in ovarian cancer diagnosis using multi-spectral photoacoustic tomography.


:In photoacoustic tomography (PAT), a tunable laser typically illuminates the tissue at multiple wavelengths, and the received photoacoustic waves are used to form functional images of relative total haemoglobin (rHbT) and blood oxygenation saturation (%sO2 ). Due to measurement errors, the estimation of these parameters can be challenging, especially in clinical studies. In this study, we use a multi-pixel method to smooth the measurements before calculating rHbT and %sO2 . We first perform phantom studies using blood tubes of calibrated %sO2 to evaluate the accuracy of our %sO2 estimation. We conclude by presenting diagnostic results from PAT of 33 patients with 51 ovarian masses imaged by our co-registered PAT and ultrasound system. The ovarian masses were divided into malignant and benign/normal groups. Functional maps of rHbT and %sO2 and their histograms as well as spectral features were calculated using the PAT data from all ovaries in these two groups. Support vector machine models were trained on different combinations of the significant features. The area under ROC (AUC) of 0.93 (0.95%CI: 0.90-0.96) on the testing data set was achieved by combining mean %sO2 , a spectral feature, and the score of the study radiologist.


J Biophotonics


Journal of biophotonics


Amidi E,Yang G,Uddin KMS,Luo H,Middleton W,Powell M,Siegel C,Zhu Q




Has Abstract


2020-12-30 00:00:00








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    abstract::A multiple exposure laser speckle contrast imaging (MELSCI) setup for visualizing blood perfusion was developed using a field programmable gate array (FPGA), connected to a 1000 frames per second (fps) 1-megapixel camera sensor. Multiple exposure time images at 1, 2, 4, 8, 16, 32 and 64 milliseconds were calculated by...

    journal_title:Journal of biophotonics

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    authors: Hultman M,Fredriksson I,Larsson M,Alvandpour A,Strömberg T

    更新日期:2018-02-01 00:00:00

  • Therapeutic prognosis of prostate cancer using surface-enhanced Raman scattering of patient urine and multivariate statistical analysis.

    abstract::Surface-enhanced Raman scattering (SERS) is highly sensitive and label-free analytical technique based on Raman spectroscopy aided by field-multiplying plasmonic nanostructures. We report the use of SERS measurements of patient urine in conjunction with biostatistical algorithms to assess the treatment response of pro...

    journal_title:Journal of biophotonics

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    authors: Ma Y,Chi J,Zheng Z,Attygalle A,Kim IY,Du H

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  • Unique spectral signature of human cutaneous squamous cell carcinoma by photoacoustic imaging.

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    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Hult J,Dahlstrand U,Merdasa A,Wickerström K,Chakari R,Persson B,Cinthio M,Erlöv T,Albinsson J,Gesslein B,Sheikh R,Malmsjö M

    更新日期:2020-05-01 00:00:00

  • Stereotaxic endoscopy for the ocular imaging of awake, freely moving animal models.

    abstract::Stereotaxic instruments are increasingly used in research animals for the study of disease, but typically require restraints and anesthetic procedures. A stereotaxic head mount that enables imaging of the anterior chamber of the eye in alert and freely mobile mice is presented in this study. The head mount is fitted b...

    journal_title:Journal of biophotonics

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    authors: Paulson B,Lee S,Jue M,Lee K,Lee S,Kim GB,Moon Y,Lee JY,Kim N,Kim JK

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  • Development of a molecular bioswitch using fluorescence lifetime imaging: Incremental activation of fluorescein diacetate.

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    journal_title:Journal of biophotonics

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    authors: Barnoy EA,Popovtzer R,Fixler D

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    abstract::Drowsy driving contributes to ~20% of all traffic accidents worldwide. Onsite monitoring the mental condition of a driver and forewarning may be a preventive solution to reduce occurrence of drowsiness and potential accidents. Functional near-infrared spectroscopy (fNIRS) has been successfully utilized in hemodynamics...

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    authors: Li T,Lin Y,Gao Y,Zhong F

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    pub_type: 杂志文章


    authors: Khamar P,Dalal R,Chandapura R,Francis M,Shetty R,Nelson EJR,Nuijts RMMA,Sinha Roy A

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  • Analgesic effect of Photobiomodulation Therapy: An in vitro and in vivo study.

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    更新日期:2019-10-01 00:00:00

  • Nanoscale distinction of membrane patches--a TERS study of Halobacterium salinarum.

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    pub_type: 杂志文章


    authors: Deckert-Gaudig T,Böhme R,Freier E,Sebesta A,Merkendorf T,Popp J,Gerwert K,Deckert V

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    authors: Janpongsri W,Huang J,Ng R,Wahl DJ,Sarunic MV,Jian Y

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    abstract::In this work we present how to entirely remove the scattering ambiguity present in existing multiphoton multifocal systems. This is achieved through the development and implementation of single-element detection systems that incorporate high-speed photon-counting electronics. These systems can be used to image entire ...

    journal_title:Journal of biophotonics

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    authors: Hoover EE,Field JJ,Winters DG,Young MD,Chandler EV,Speirs JC,Lapenna JT,Kim SM,Ding SY,Bartels RA,Wang JW,Squier JA

    更新日期:2012-05-01 00:00:00

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    pub_type: 杂志文章


    authors: Liu M,Liu X,Huang Z,Tang X,Lin X,Xu Y,Chen G,Kwok HF,Lin Y,Feng S

    更新日期:2019-03-01 00:00:00

  • Monitoring caffeine and resveratrol cutaneous permeation by confocal Raman microspectroscopy.

    abstract::Dynamic follow-up of exogenous molecules permeation through the skin is one among many competing applications for confocal Raman microspectroscopy. Previous studies showed the feasibility of tracking actives through the skin; the next step should be recording in vivo kinetics. Thus, we conducted a study to evaluate th...

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    authors: Tfaili S,Josse G,Angiboust JF,Manfait M,Piot O

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    abstract::The photodetachment of Br(-), I(-) and OH(-) in aqueous solution is studied by 2- and 3-pulse femtosecond spectroscopy. The UV excitation leads to fast electron separation followed by formation of a donor-electron pairs. An additional repump pulse is used for secondary excitation of the intermediates. The 3-pulse tech...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Fischer MK,Gliserin A,Laubereau A,Iglev H

    更新日期:2011-03-01 00:00:00

  • Imaging depth variations in hyperspectral imaging: Development of a method to detect tumor up to the required tumor-free margin width.

    abstract::Hyperspectral imaging is a promising technique for resection margin assessment during cancer surgery. Thereby, only a specific amount of the tissue below the resection surface, the clinically defined margin width, should be assessed. Since the imaging depth of hyperspectral imaging varies with wavelength and tissue co...

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    authors: Kho E,de Boer LL,Post AL,Van de Vijver KK,Jóźwiak K,Sterenborg HJCM,Ruers TJM

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  • Impact of cell division on intracellular uptake and nuclear targeting with fluorescent SiC-based nanoparticles.

    abstract::Semiconductor nanoparticles (NPs) became important and wide-used tool for cell imaging because of their unique remarkable properties. Nevertheless, all previous investigations in this area were done on proliferating cells. For the first time, this work demonstrates strong influence of cell active proliferation/contact...

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    authors: Serdiuk T,Lysenko V,Mognetti B,Skryshevsky V,Géloën A

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    abstract::FTIR spectroscopy is a powerful diagnostic tool that can also derive biochemical signatures of a wide range of cellular materials, such as cytology, histology, live cells, and biofluids. However, while classification is a well-established subject, biomarker identification lacks standards and validation of its methods....

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Trevisan J,Park J,Angelov PP,Ahmadzai AA,Gajjar K,Scott AD,Carmichael PL,Martin FL

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    abstract::Surface-enhanced Raman scattering (SERS) spectroscopy possesses the most promising advantage of multiplex detection for biosensing applications, which is achieved due to the narrow 'fingerprint' Raman spectra from the analyte molecules. We developed an ultrasensitive platform for the multiplex detection of cancer biom...

    journal_title:Journal of biophotonics

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    authors: Dinish US,Balasundaram G,Chang YT,Olivo M

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  • Utilizing lymphatic cell markers to visualize human lymphatic abnormalities.

    abstract::In vivo visualization of the human lymphatic system is limited by the mode of delivery of tracing agents, depth of field and size of the area examined, and specificity of the cell markers used to distinguish lymphatic endothelium from the blood vessels and the surrounding tissues. These limitations are particularly pr...

    journal_title:Journal of biophotonics

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    authors: Lokmic Z

    更新日期:2018-08-01 00:00:00

  • Diagnosis of liver cancer from blood sera using FTIR microspectroscopy: a preliminary study.

    abstract::FTIR microspectroscopy was applied for studying macromolecular changes in human serum samples from patients with healthy livers, and those diagnosed with liver cirrhosis or hepatocellular carcinoma (HCC). Our study demonstrated that the serum samples from HCC and cirrhotic patients could readily be discriminated from ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Thumanu K,Sangrajrang S,Khuhaprema T,Kalalak A,Tanthanuch W,Pongpiachan S,Heraud P

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  • Confocal laser endomicroscopy in head and neck malignancies using FITC-labelled EpCAM- and EGF-R-antibodies in cell lines and tumor biopsies.

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    authors: Englhard AS,Palaras A,Volgger V,Stepp H,Mack B,Libl D,Gires O,Betz CS

    更新日期:2017-10-01 00:00:00

  • Biocompatible astaxanthin as novel contrast agent for biomedical imaging.

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    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Nguyen VP,Park S,Oh J,Wook Kang H

    更新日期:2017-08-01 00:00:00

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    authors: Abdolmanafi A,Cheriet F,Duong L,Ibrahim R,Dahdah N

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  • FTIR protein secondary structure analysis of human ascending aortic tissues.

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    authors: Bonnier F,Rubin S,Debelle L,Ventéo L,Pluot M,Baehrel B,Manfait M,Sockalingum GD

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    authors: Kassab G,Cheburkanov V,Willis J,Moule MG,Kurachi C,Yakovlev V,Cirillo JD,Bagnato VS

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    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Engel KW,Khan I,Arany PR

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    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Guo H,Gao L,Yu J,He X,Wang H,Zheng J,Yang X

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    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Steelman Z,Meng Z,Traverso AJ,Yakovlev VV

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    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Lilge L,Manalac A,Weersink M,Schwiegelshohn F,Young-Schultz T,Abdalrhman AS,Wang C,Ngan A,Gu FX,Betz V,Hofmann R

    更新日期:2020-12-01 00:00:00