Abstract:
:In this work we present how to entirely remove the scattering ambiguity present in existing multiphoton multifocal systems. This is achieved through the development and implementation of single-element detection systems that incorporate high-speed photon-counting electronics. These systems can be used to image entire volumes in the time it takes to perform a single transverse scan (four depths simultaneously at a rate of 30 Hz). In addition, this capability is further exploited to accomplish single-element detection of multiple modalities (two photon excited fluorescence and second harmonic generation) and to perform efficient image deconvolution. Finally, we demonstrate a new system that promises to significantly simplify this promising technology.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Hoover EE,Field JJ,Winters DG,Young MD,Chandler EV,Speirs JC,Lapenna JT,Kim SM,Ding SY,Bartels RA,Wang JW,Squier JAdoi
10.1002/jbio.201100139subject
Has Abstractpub_date
2012-05-01 00:00:00pages
425-36issue
5-6eissn
1864-063Xissn
1864-0648journal_volume
5pub_type
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