Abstract:
:Myelin is a key component of the peripheral nervous system, whose structure anomaly in the digital skin is implicated in neuropathy. Here we demonstrate an in vivo labeling and imaging technique, capable of visualizing myelin sheaths deep in the mouse digital skin. Through material characterization, we verify that 3-photon fluorescence (3PF) can be generated from a commonly used dye- FluoroMyelin Red for labeling myelin, excited at the 1700-nm window. Topical injection of FluoroMyelin Red in the mouse digit leads to bright labeling of myelin sheaths. Harnessing the deep-penetration capability of 3-photon microscopy excited at the 1700-nm window, we demonstrate that 3PF imaging of FluoroMyelin Red-labeled myelin sheaths in the mouse digit in vivo can be achieved to a depth 340 μm below the skin surface, revealing both branching bundle of and individual myelin sheaths.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
He C,Deng X,Pan Y,Tong S,Kang J,Li J,Qiu P,Wang Kdoi
10.1002/jbio.202000321subject
Has Abstractpub_date
2020-12-01 00:00:00pages
e202000321issue
12eissn
1864-063Xissn
1864-0648journal_volume
13pub_type
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