Confocal laser endomicroscopy in head and neck malignancies using FITC-labelled EpCAM- and EGF-R-antibodies in cell lines and tumor biopsies.


:Intraoperative detection of residual malignant cells at tumor margins following excision of primary tumors could help improving surgery and thus patients' outcome. The feasibility of the tumor antigens epidermal growth factor receptor (EGF-R) and epithelial cell adhesion molecule (EpCAM) for antibody-dependent confocal laser scanning endomicroscopy (CLE)-mediated visualization of malignant cells was addressed. Both tumor antigens are highly and frequently expressed in the majority of carcinomas, including head and neck squamous cell carcinomas (HNSCC), and represent prognostic and therapeutic tumor target molecules. FITC-conjugated EGF-R- and EpCAM-specific antibodies served as molecular tools for the detection of antigen-positive cells using the CLE technology. Specificity of both antibodies and their ability to discriminate tumor from non-tumor cells were assessed in vitro with human fibroblasts and PCI-1 HNSCC cell lines, and ex vivo on primary HNSCC samples (n = 11) and healthy mucosa (n = 5). Antigen specificity of the used EpCAM-specific antibody was superior to that of the EGF-R-specific antibody both in vitro and ex vivo (100% vs. 31.25%), and allowed visualization of cellular structures in CLE measurements. These results hold promise for possible future applications in humans.


J Biophotonics


Journal of biophotonics


Englhard AS,Palaras A,Volgger V,Stepp H,Mack B,Libl D,Gires O,Betz CS




Has Abstract


2017-10-01 00:00:00












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    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Bydlon TM,Nachabé R,Ramanujam N,Sterenborg HJ,Hendriks BH

    更新日期:2015-01-01 00:00:00

  • Quantification of mucosa oxygenation using three discrete spectral bands of visible light.

    abstract::Quantification of the mucosa oxygenation levels during Endoscopic imaging provides useful physiological/diagnostic information. In this work a method for non-contact quantification of the oxygen saturation index during Endoscopic imaging using three discrete spectral-band in the blue, the green, and the red parts of t...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Fawzy Y

    更新日期:2009-12-01 00:00:00

  • Optical clearing for multiscale biological tissues.

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    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Yu T,Qi Y,Gong H,Luo Q,Zhu D

    更新日期:2018-02-01 00:00:00

  • Utilizing lymphatic cell markers to visualize human lymphatic abnormalities.

    abstract::In vivo visualization of the human lymphatic system is limited by the mode of delivery of tracing agents, depth of field and size of the area examined, and specificity of the cell markers used to distinguish lymphatic endothelium from the blood vessels and the surrounding tissues. These limitations are particularly pr...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Lokmic Z

    更新日期:2018-08-01 00:00:00

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    abstract::Optical coherence tomography (OCT) imaging shows a significant potential in clinical routines due to its noninvasive property. However, the quality of OCT images is generally limited by inherent speckle noise of OCT imaging and low sampling rate. To obtain high signal-to-noise ratio (SNR) and high-resolution (HR) OCT ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Qiu B,You Y,Huang Z,Meng X,Jiang Z,Zhou C,Liu G,Yang K,Ren Q,Lu Y

    更新日期:2021-01-01 00:00:00

  • A simple approach for non-invasive transcranial optical vascular imaging (nTOVI).

    abstract::In vivo imaging of cerebral vasculature is highly vital for clinicians and medical researchers alike. For a number of years non-invasive optical-based imaging of brain vascular network by using standard fluorescence probes has been considered as impossible. In the current paper controverting this paradigm, we present ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Kalchenko V,Israeli D,Kuznetsov Y,Meglinski I,Harmelin A

    更新日期:2015-11-01 00:00:00

  • Retraction: Zhong H, Lu L, Wang Y, Yang H. Single layer molybdenum disulfide as an optical nanoprobe for 2 photon luminescence and second harmonic generation cell imaging. J. Biophotonics. 2018;11:e201700354.

    abstract::The above article from the Journal of Biophotonics, published online on 9 January 2018 in Wiley Online Library as Early View (, and in Volume 11, e201700354, has been retracted by agreement between the authors (Hong Zhong, Lin Lu, Yuanhao Wang, Hongxing Ya...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,撤回出版物



    更新日期:2020-04-01 00:00:00

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    journal_title:Journal of biophotonics

    pub_type: 社论


    authors: Vukosavljevic B,Hittinger M,Hachmeister H,Pilger C,Murgia X,Gepp MM,Gentile L,Huwer H,Schneider-Daum N,Huser T,Lehr CM,Windbergs M

    更新日期:2019-06-01 00:00:00

  • A representation learning approach for recovering scatter-corrected spectra from Fourier-transform infrared spectra of tissue samples.

    abstract::Infrared spectra obtained from cell or tissue specimen have commonly been observed to involve a significant degree of scattering effects, often Mie scattering, which probably overshadows biochemically relevant spectral information by a nonlinear, nonadditive spectral component in Fourier transform infrared (FTIR) spec...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Raulf AP,Butke J,Menzen L,Küpper C,Großerueschkamp F,Gerwert K,Mosig A

    更新日期:2020-12-08 00:00:00

  • In vivo sun protection factor and UVA protection factor determination using (hybrid) diffuse reflectance spectroscopy and a multi-lambda-LED light source.

    abstract::The sun protection factor (SPF) values are currently determined using an invasive procedure, in which the volunteers are irradiated with ultraviolet (UV) light. Non-invasive approaches based on hybrid diffuse reflectance spectroscopy (HDRS) have shown a good correlation with conventional SPF testing. Here, we present ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Throm CM,Wiora G,Reble C,Schleusener J,Schanzer S,Karrer H,Kolbe L,Khazaka G,Meinke MC,Lademann J

    更新日期:2020-10-06 00:00:00

  • Development of a molecular bioswitch using fluorescence lifetime imaging: Incremental activation of fluorescein diacetate.

    abstract::Molecular bioswitches offer an invaluable asset in the shift from systemic to targeted treatments. Within the growing arsenal of switches are imaging probes that functionalize only in given locations or situations. Acetate esters are a common fluorescent example, known to activate upon interaction with esterases. Fluo...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Barnoy EA,Popovtzer R,Fixler D

    更新日期:2018-02-01 00:00:00

  • CTAB enhancement of FRET in DNA structures.

    abstract::The effect of cetyl-trimethylammonium bromide (CTAB) on enhancing the fluorescence resonance energy transfer (FRET) between two dye-conjugated DNA strands was studied using fluorescence emission spectroscopy and dynamic light scattering (DLS). For hybridized DNA where one strand is conjugated with a TAMRA donor and th...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Oh T,Takahashi T,Kim S,Heller MJ

    更新日期:2016-01-01 00:00:00

  • Visualizing the "sandwich" structure of osteocytes in their native environment deep in bone in vivo.

    abstract::Osteocytes are the most abundant cells in bone and always the focus of bone research. They are embedded in the highly scattering mineralized bone matrix. Consequently, visualizing osteocytes deep in bone with subcellular resolution poses a major challenge for in vivo bone research. Here we overcome this challenge by d...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Wang K,Du Y,Liu H,Gan M,Tong S,Wen W,Zhuang Z,Qiu P

    更新日期:2019-04-01 00:00:00

  • Laser imaging polarimetry of nacre.

    abstract::Nacre is a complex biomaterial made of aragonite-tablet bricks and organic mortar that is considerably resilient against breakage. Nacre has been studied with a wide range of laboratory techniques, leading to understanding key fundamentals and informing the creation of bio-inspired materials. In this article, we prese...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Jones JA,Metzler RA,D'Addario AJ,Burgess C,Regan B,Spano S,Cvarch BA,Galvez EJ

    更新日期:2018-10-01 00:00:00

  • Potentially malignant lesions of the upper aerodigestive tract.

    abstract::Mucosal lesions of the upper aerodigestive tract usually concern the squamous epithelium of the surface. Many precancerous alterations now designated as potentially malignant are caused by DNA damage resulting from long term exposure to tobacco and alcohol consumption. The distinction from conditions with a similar ph...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Dreyer T,Kreisel M

    更新日期:2012-04-01 00:00:00

  • Application of corneal tomography before keratorefractive procedure for laser vision correction.

    abstract::Ectasia after refractive surgery represents a major concern among refractive surgeons. Corneal abnormalities and preexisting corneal ectasia are the most important risk factors. Corneal topography and central corneal thickness are the factors traditionally screening for in refractive surgery candidates. Study of the a...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Luz A,Lopes B,Salomão M,Ambrósio R

    更新日期:2016-05-01 00:00:00

  • Longtime driving induced cerebral hemodynamic elevation and behavior degradation as assessed by functional near-infrared spectroscopy and a voluntary attention test.

    abstract::Drowsy driving contributes to ~20% of all traffic accidents worldwide. Onsite monitoring the mental condition of a driver and forewarning may be a preventive solution to reduce occurrence of drowsiness and potential accidents. Functional near-infrared spectroscopy (fNIRS) has been successfully utilized in hemodynamics...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Li T,Lin Y,Gao Y,Zhong F

    更新日期:2018-12-01 00:00:00

  • The metabolic component of cellular refractivity and its importance for optical cytometry.

    abstract::Initially, it has been shown that the phase thickness and refractivity (the latter interpreted as the difference of the refractivity indices of an object and surrounding medium) depend on the functional state of mitochondria. The refractivity of various objects decreased in response to energy depletion. This dependenc...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Tychinsky V

    更新日期:2009-09-01 00:00:00

  • Laser exposure of gold nanorods can induce intracellular calcium transients.

    abstract::Uncoated and poly(styrene sulphonate) (PSS)-coated gold nanorods were taken up by NG108-15 neuronal cells. Exposure to 780 nm laser light at the plasmon resonance wavelength of the gold nanorods was found to induce intracellular Ca(2+) transients. The higher Ca(2+) peaks were observed at lower laser doses, with the hi...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Paviolo C,Haycock JW,Cadusch PJ,McArthur SL,Stoddart PR

    更新日期:2014-10-01 00:00:00

  • SPR-based plastic optical fibre biosensor for the detection of C-reactive protein in serum.

    abstract::A plastic optical fibre biosensor based on surface plasmon resonance for the detection of C-reactive protein (CRP) in serum is proposed. The biosensor was integrated into a home-made thermo-stabilized microfluidic system that allows avoiding any thermal and/or mechanical fluctuation and maintaining the best stable con...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Aray A,Chiavaioli F,Arjmand M,Trono C,Tombelli S,Giannetti A,Cennamo N,Soltanolkotabi M,Zeni L,Baldini F

    更新日期:2016-10-01 00:00:00

  • Activity assessment of root caries lesions with thermal and near-IR imaging methods.

    abstract::The purpose of this study was to evaluate thermal and near-infrared (NIR) reflectance imaging methods for the assessment of the activity of root caries lesions. In addition, changes in the lesion structure were monitored with polarization sensitive optical coherence tomography (PS-OCT). Artificial bovine and natural r...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Lee RC,Darling CL,Staninec M,Ragadio A,Fried D

    更新日期:2017-03-01 00:00:00

  • Tracking heavy-water-incorporated confocal Raman spectroscopy for evaluating the effects of PEGylated emulsifiers on skin barrier.

    abstract::The class of PEGylated emulsifiers finds broad application in the pharmaceutical and cosmetic industry. We target on one of the categories of polyethylene glycol (PEG) alkyl ethers with different lipophilic and hydrophilic chain length and aim to examine their effects on the skin comprehensively. In this study, we emp...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Liu Y,Lunter DJ

    更新日期:2020-12-01 00:00:00

  • Nanofluidic devices towards single DNA molecule sequence mapping.

    abstract::Nanofluidics enables the imaging of stretched single molecules with potential applications for single molecule sequence mapping. Lab-on-a-chip devices for single cell trapping and lyzing, genomic DNA extraction from single cells, and optical mapping of genomic length DNA has been demonstrated separately. Yet the pursu...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Marie R,Kristensen A

    更新日期:2012-08-01 00:00:00

  • Monitoring the biochemical changes occurring to human keratinocytes exposed to solar radiation by Raman spectroscopy.

    abstract::Solar radiation exposure is recognised to be a significant contributor to the development of skin cancer. Monitoring the simultaneous and consecutive mechanisms of interaction could provide a greater understanding of the process of photocarcinogenesis. This work presents an analysis of the biochemical and morphologica...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Lopez-Gonzalez U,Casey A,J Byrne H

    更新日期:2020-10-24 00:00:00

  • In vivo pump-probe optical coherence tomography imaging in Xenopus laevis.

    abstract::Currently, optical coherence tomography (OCT), is not capable of obtaining molecular information often crucial for identification of disease. To enable molecular imaging with OCT, we have further developed a technique that harnesses transient changes in light absorption in the sample to garner molecular information. A...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Carrasco-Zevallos O,Shelton RL,Kim W,Pearson J,Applegate BE

    更新日期:2015-01-01 00:00:00

  • Impact of cell division on intracellular uptake and nuclear targeting with fluorescent SiC-based nanoparticles.

    abstract::Semiconductor nanoparticles (NPs) became important and wide-used tool for cell imaging because of their unique remarkable properties. Nevertheless, all previous investigations in this area were done on proliferating cells. For the first time, this work demonstrates strong influence of cell active proliferation/contact...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Serdiuk T,Lysenko V,Mognetti B,Skryshevsky V,Géloën A

    更新日期:2013-03-01 00:00:00

  • A robust ex vivo method to evaluate the diffusion properties of agents in biological tissues.

    abstract::A robust method is presented for evaluating the diffusion properties of chemicals in ex vivo biological tissues. Using this method that relies only on thickness and collimated transmittance measurements, the diffusion properties of glycerol, fructose, polypropylene glycol and water in muscle tissues were evaluated. Am...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Carneiro I,Carvalho S,Henrique R,Oliveira LM,Tuchin VV

    更新日期:2019-04-01 00:00:00

  • Characterization of a multiple particle delivery complex and determination of cellular photodamage in skin fibroblast and breast cancer cell lines.

    abstract::Zinc metallized Phthalocyanine (ZnPcSmix ), a potent photosensitizer, is conjugated to gold dendrimer encapsulated nanoparticles (AuDENPs) in order to improve the efficacy of photodynamic therapy (PDT) using MCF-7 breast cancer cells and WS1 fibroblast cells as a control. Both ZnPcSmix and AuDENPs are mixed in a nitro...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Mfouo-Tynga I,Houreld NN,Abrahamse H

    更新日期:2018-02-01 00:00:00

  • Photosensitized singlet oxygen generation and detection: Recent advances and future perspectives in cancer photodynamic therapy.

    abstract::Photodynamic therapy (PDT) uses photosensitizers and visible light in combination with molecular oxygen to produce reactive oxygen species (ROS) that kill malignant cells by apoptosis and/or necrosis, shut down the tumor microvasculature and stimulate the host immune system. The excited singlet state of oxygen (1 O2 )...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Li B,Lin L,Lin H,Wilson BC

    更新日期:2016-12-01 00:00:00

  • Intracellular localization and delivery of plasmid DNA by biodegradable microsphere-mediated femtosecond laser optoporation.

    abstract::Micro-/nanosphere-mediated femtosecond laser cell perforation is one of the high throughput technologies used for macro-molecule-delivery into multiple cells. We have demonstrated the delivery of plasmid-DNA/liposome complexes into cells using biodegradable polymer microspheres and a femtosecond laser and investigated...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Ishii A,Hiruta Y,Heinemann D,Heisterkamp A,Kanazawa H,Terakawa M

    更新日期:2017-12-01 00:00:00