Rapid discrimination of colon cancer cells with single base mutation in KRAS gene segment using laser tweezers Raman spectroscopy.


:Laser tweezers Raman spectroscopy (LTRS) as a label-free and noninvasive technology has been proven to be an ideal tool for analysis of single living cells, which provides important fingerprint information without interference from surrounding environments. For the first time, LTRS system was successfully used to examine the colon cancer cells with single base mutation in KRAS gene segment, including DKS-8 (KRAS wild-type [WT]) and DLD-1 (KRAS mutant-type [MT]), HKE-3 (KRAS WT) and HCT-116 (KRAS MT). Spectra changes of some vital biomolecules due to the gene mutation state were sensitively recorded by our home-made LTRS system. As a result of the comparison between DKS-8 and DLD-1 cells, an index of 97.5% of correct classification was obtained by combining LTRS with principle component analysis coupled with linear discriminant analysis (PCA-LDA) statistical analysis, while an index of 97.0% of correct classification was achieved between HKE-3 and HCT-116 cells. Moreover, between WT cells (DKS-8 and HKE-3) vs MT cells (DLD-1 and HCT-116), the index of correct classification was 81.2%, which was quite encouraging. Our preliminary results showed that the LTRS system coupled with PCA-LDA analysis will have a great potential for further applications in the rapid and label-free detection of circulating tumor cells in liquid biopsy.


J Biophotonics


Journal of biophotonics


Liu M,Liu X,Huang Z,Tang X,Lin X,Xu Y,Chen G,Kwok HF,Lin Y,Feng S




Has Abstract


2019-03-01 00:00:00












  • Stain-free interferometric phase microscopy correlation with DNA fragmentation stain in human spermatozoa.

    abstract::Acridine orange (AO) staining is used to diagnose DNA fragmentation status in sperm cells. Interferometric phase microscopy (IPM) is an optical imaging method based on digital holographic microscopy that provides quantitative morphological and refractive index imaging of cells in vitro without the need for staining. W...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Barnea I,Karako L,Mirsky SK,Levi M,Balberg M,Shaked NT

    更新日期:2018-11-01 00:00:00

  • Multispectral optoacoustic tomography of muscle perfusion and oxygenation under arterial and venous occlusion: A human pilot study.

    abstract::Perfusion and oxygenation are critical parameters of muscle metabolism in health and disease. They have been both the target of many studies, in particular using near-infrared spectroscopy (NIRS). However, difficulties with quantifying NIRS signals have limited a wide dissemination of the method to the clinics. Our ai...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Karlas A,Kallmayer M,Fasoula NA,Liapis E,Bariotakis M,Krönke M,Anastasopoulou M,Reber J,Eckstein HH,Ntziachristos V

    更新日期:2020-06-01 00:00:00

  • The differentiation of oral soft- and hard tissues using laser induced breakdown spectroscopy - a prospect for tissue specific laser surgery.

    abstract::Compared to conventional techniques, Laser surgery procedures provide a number of advantages, but may be associated with an increased risk of iatrogenic damage to important anatomical structures. The type of tissue ablated in the focus spot is unknown. Laser-Induced Breakdown-Spectroscopy (LIBS) has the potential to g...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Rohde M,Mehari F,Klämpfl F,Adler W,Neukam FW,Schmidt M,Stelzle F

    更新日期:2017-10-01 00:00:00

  • Hyperspectral optical coherence tomography for in vivo visualization of melanin in the retinal pigment epithelium.

    abstract::Previous studies for melanin visualization in the retinal pigment epithelium (RPE) have exploited either its absorption properties (using photoacoustic tomography or photothermal optical coherence tomography [OCT]) or its depolarization properties (using polarization sensitive OCT). However, these methods are only sui...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Harper DJ,Konegger T,Augustin M,Schützenberger K,Eugui P,Lichtenegger A,Merkle CW,Hitzenberger CK,Glösmann M,Baumann B

    更新日期:2019-12-01 00:00:00

  • Chromophore based analyses of steady-state diffuse reflectance spectroscopy: current status and perspectives for clinical adoption.

    abstract::Diffuse reflectance spectroscopy is a rapidly growing technology in the biophotonics community where it has shown promise in its ability to classify different tissues. In the steady-state domain a wide spectrum of clinical applications is supported with this technology ranging from diagnostic to guided interventions. ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Bydlon TM,Nachabé R,Ramanujam N,Sterenborg HJ,Hendriks BH

    更新日期:2015-01-01 00:00:00

  • Spatial mapping of affinity changes for the integrin LFA-1 during cell migration using clusters identified based on local density.

    abstract::Localization microscopy methods like Stochastic Optical Reconstruction Microscopy (STORM) are very well suited for exploring clustering of proteins, as the data inherently provide a list of molecular coordinates. Here we use state-of-art cluster analysis algorithms (DBSCAN) to explore the clustering behaviour of diffe...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Persson H,Potrzebowski W,Potrzebowska K,Svensson LM

    更新日期:2019-03-01 00:00:00

  • A 15.6 frames per second 1-megapixel multiple exposure laser speckle contrast imaging setup.

    abstract::A multiple exposure laser speckle contrast imaging (MELSCI) setup for visualizing blood perfusion was developed using a field programmable gate array (FPGA), connected to a 1000 frames per second (fps) 1-megapixel camera sensor. Multiple exposure time images at 1, 2, 4, 8, 16, 32 and 64 milliseconds were calculated by...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Hultman M,Fredriksson I,Larsson M,Alvandpour A,Strömberg T

    更新日期:2018-02-01 00:00:00

  • Autofocusing technologies for whole slide imaging and automated microscopy.

    abstract::Whole slide imaging (WSI) has moved digital pathology closer to diagnostic practice in recent years. Due to the inherent tissue topography variability, accurate autofocusing remains a critical challenge for WSI and automated microscopy systems. The traditional focus map surveying method is limited in its ability to ac...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Bian Z,Guo C,Jiang S,Zhu J,Wang R,Song P,Zhang Z,Hoshino K,Zheng G

    更新日期:2020-12-01 00:00:00

  • Confocal laser endomicroscopy in head and neck malignancies using FITC-labelled EpCAM- and EGF-R-antibodies in cell lines and tumor biopsies.

    abstract::Intraoperative detection of residual malignant cells at tumor margins following excision of primary tumors could help improving surgery and thus patients' outcome. The feasibility of the tumor antigens epidermal growth factor receptor (EGF-R) and epithelial cell adhesion molecule (EpCAM) for antibody-dependent confoca...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Englhard AS,Palaras A,Volgger V,Stepp H,Mack B,Libl D,Gires O,Betz CS

    更新日期:2017-10-01 00:00:00

  • Towards Raman spectroscopy of urine as screening tool.

    abstract::For the screening purposes urine is an especially attractive biofluid, since it offers easy and noninvasive sample collection and provides a snapshot of the whole metabolic status of the organism, which may change under different pathological conditions. Raman spectroscopy (RS) has the potential to monitor these chang...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Žukovskaja O,Ryabchykov O,Straßburger M,Heinekamp T,Brakhage AA,Hennings CJ,Hübner CA,Wegmann M,Cialla-May D,Bocklitz TW,Weber K,Popp J

    更新日期:2020-01-01 00:00:00

  • Inverse SORS for detecting a low Raman-active turbid sample placed inside a highly Raman-active diffusely scattering matrix - A feasibility study.

    abstract::The broad range of applications of spatially-offset Raman spectroscopy (SORS) were found to involve samples having only marginal differences in Raman cross-sections between the surface and subsurface targets. We report the results of a feasibility study to evaluate the potential of the approach to identify the presenc...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Khan KM,Dutta SB,Krishna H,Majumder SK

    更新日期:2016-09-01 00:00:00

  • Optical investigations of the RNA polymerase molecular motor.

    abstract::Molecular motors have been extensively studied over the past 25 years using a range of optical microscopy methods. The specific example of RNA polymerase, responsible for transcribing the information encoded in DNA into RNA, illustrates the strength of the convergence between these recent approaches and the vast store...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Strick TR

    更新日期:2008-09-01 00:00:00

  • Brillouin spectroscopy as a new method of screening for increased CSF total protein during bacterial meningitis.

    abstract::Bacterial meningitis is a disease of pronounced clinical significance, especially in the developing world. Immediate treatment with antibiotics is essential, and no single test can provide a conclusive diagnosis. It is well established that elevated total protein in cerebrospinal fluid (CSF) is associated with bacteri...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Steelman Z,Meng Z,Traverso AJ,Yakovlev VV

    更新日期:2015-05-01 00:00:00

  • Photoacoustically-guided photothermal killing of mosquitoes targeted by nanoparticles.

    abstract::In biomedical applications, nanoparticles have demonstrated the potential to eradicate abnormal cells in small localized pathological zones associated with cancer or infections. Here, we introduce a method for nanotechnology-based photothermal (PT) killing of whole organisms considered harmful to humans or the environ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Foster SR,Galanzha EI,Totten DC,Beneš H,Shmookler Reis RJ,Zharov VP

    更新日期:2014-07-01 00:00:00

  • Analysis of basidiomycete pigments in situ by Raman spectroscopy.

    abstract::Basidiomycetes, that is, mushroom-type fungi, are known to produce pigments in response to environmental impacts. As antioxidants with a high level of unsaturation, these compounds can neutralize highly oxidative species. In the event of close contact with other microbes, the enzymatically controlled pigment productio...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Tauber JP,Matthäus C,Lenz C,Hoffmeister D,Popp J

    更新日期:2018-06-01 00:00:00

  • Modern trends in biophotonics for clinical diagnosis and therapy to solve unmet clinical needs.

    abstract::This contribution covers recent original research papers in the biophotonics field. The content is organized into main techniques such as multiphoton microscopy, Raman spectroscopy, infrared spectroscopy, optical coherence tomography and photoacoustic tomography, and their applications in the context of fluid, cell, t...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Krafft C

    更新日期:2016-12-01 00:00:00

  • Ultrafast electron transfer processes studied by pump-repump-probe spectroscopy.

    abstract::The photodetachment of Br(-), I(-) and OH(-) in aqueous solution is studied by 2- and 3-pulse femtosecond spectroscopy. The UV excitation leads to fast electron separation followed by formation of a donor-electron pairs. An additional repump pulse is used for secondary excitation of the intermediates. The 3-pulse tech...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Fischer MK,Gliserin A,Laubereau A,Iglev H

    更新日期:2011-03-01 00:00:00

  • A simple approach for non-invasive transcranial optical vascular imaging (nTOVI).

    abstract::In vivo imaging of cerebral vasculature is highly vital for clinicians and medical researchers alike. For a number of years non-invasive optical-based imaging of brain vascular network by using standard fluorescence probes has been considered as impossible. In the current paper controverting this paradigm, we present ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Kalchenko V,Israeli D,Kuznetsov Y,Meglinski I,Harmelin A

    更新日期:2015-11-01 00:00:00

  • Eliminating the scattering ambiguity in multifocal, multimodal, multiphoton imaging systems.

    abstract::In this work we present how to entirely remove the scattering ambiguity present in existing multiphoton multifocal systems. This is achieved through the development and implementation of single-element detection systems that incorporate high-speed photon-counting electronics. These systems can be used to image entire ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Hoover EE,Field JJ,Winters DG,Young MD,Chandler EV,Speirs JC,Lapenna JT,Kim SM,Ding SY,Bartels RA,Wang JW,Squier JA

    更新日期:2012-05-01 00:00:00

  • High fluence light emitting diode-generated red light modulates characteristics associated with skin fibrosis.

    abstract::Skin fibrosis, often referred to as skin scarring, is a significant international health problem with limited treatment options. The hallmarks of skin fibrosis are increased fibroblast proliferation, collagen production, and migration speed. Recently published clinical observations indicate that visible red light may ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Mamalis A,Koo E,Garcha M,Murphy WJ,Isseroff RR,Jagdeo J

    更新日期:2016-12-01 00:00:00

  • Minimally invasive non-thermal laser technology using laser-induced optical breakdown for skin rejuvenation.

    abstract::We describe a novel, minimally invasive laser technology for skin rejuvenation by creating isolated microscopic lesions within tissue below the epidermis using laser induced optical breakdown. Using an in-house built prototype device, tightly focused near-infrared laser pulses are used to create optical breakdown in t...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Habbema L,Verhagen R,Van Hal R,Liu Y,Varghese B

    更新日期:2012-02-01 00:00:00

  • Assesment of apoptosis induced changes in scattering using optical coherence tomography.

    abstract::The aim of this study is to identify changes in scattering with optical coherence tomography (OCT) and relate these measurements with mitochondrial changes during the initiation of apoptosis. Human retinal pigment epithelial cells were cultured and apoptosis was induced using 10% alcohol. Using the attenuation coeffic...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: de Bruin DM,Broekgaarden M,van Gemert MJ,Heger M,de la Rosette JJ,Van Leeuwen TG,Faber DJ

    更新日期:2016-09-01 00:00:00

  • Optical coherence elastography for tissue characterization: a review.

    abstract::Optical coherence elastography (OCE) represents the frontier of optical elasticity imaging techniques and focuses on the micro-scale assessment of tissue biomechanics in 3D that is hard to achieve with traditional elastographic methods. Benefit from the advancement of optical coherence tomography, and driven by the in...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Wang S,Larin KV

    更新日期:2015-04-01 00:00:00

  • Real-time Raman and SRS imaging of living human macrophages reveals cell-to-cell heterogeneity and dynamics of lipid uptake.

    abstract::Monitoring living cells in real-time is important in order to unravel complex dynamic processes in life sciences. In particular the dynamics of initiation and progression of degenerative diseases is intensely studied. In atherosclerosis the thickening of arterial walls is related to high lipid levels in the blood stre...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Stiebing C,Meyer T,Rimke I,Matthäus C,Schmitt M,Lorkowski S,Popp J

    更新日期:2017-09-01 00:00:00

  • Long-distance laser propulsion and deformation- monitoring of cells in optofluidic photonic crystal fiber.

    abstract::We introduce a unique method for laser-propelling individual cells over distances of 10s of cm through stationary liquid in a microfluidic channel. This is achieved by using liquid-filled hollow-core photonic crystal fiber (HC-PCF). HC-PCF provides low-loss light guidance in a well-defined single mode, resulting in hi...

    journal_title:Journal of biophotonics

    pub_type: 社论


    authors: Unterkofler S,Garbos MK,Euser TG,St J Russell P

    更新日期:2013-09-01 00:00:00

  • A telemetric light delivery system for metronomic photodynamic therapy (mPDT) in rats.

    abstract::Light delivery and monitoring during photodynamic therapy (PDT) is often limited by the need for a physical link between the light source, detectors and the treatment volume. This paper reports on the first in vivo experiments performed with a fully implantable telemetric system, designed for a rat glioblastoma model....

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: van Zaane F,Subbaiyan D,van der Ploeg-van den Heuvel A,de Bruijn HS,Balbas EM,Pandraud G,Sterenborg HJ,French PJ,Robinson DJ

    更新日期:2010-06-01 00:00:00

  • Microfluidics and photonics for Bio-System-on-a-Chip: a review of advancements in technology towards a microfluidic flow cytometry chip.

    abstract::Microfluidics and photonics come together to form a field commonly referred to as 'optofluidics'. Flow cytometry provides the field with a technology base from which both microfluidic and photonic components be developed and integrated into a useful device. This article reviews some of the more recent developments to ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Godin J,Chen CH,Cho SH,Qiao W,Tsai F,Lo YH

    更新日期:2008-10-01 00:00:00

  • Unique spectral signature of human cutaneous squamous cell carcinoma by photoacoustic imaging.

    abstract::Cutaneous squamous cell carcinoma (cSCC) is a common skin cancer with metastatic potential. To reduce reoperations due to nonradical excision, there is a need to develop a technique for identification of tumor margins preoperatively. Photoacoustic (PA) imaging is a novel imaging technology that combines the strengths ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Hult J,Dahlstrand U,Merdasa A,Wickerström K,Chakari R,Persson B,Cinthio M,Erlöv T,Albinsson J,Gesslein B,Sheikh R,Malmsjö M

    更新日期:2020-05-01 00:00:00

  • Imaging engineered tissues using structural and functional optical coherence tomography.

    abstract::As the field of tissue engineering evolves, there will be an increasingly important need to visualize and track the complex dynamic changes that occur within three-dimensional constructs. Optical coherence tomography (OCT), as an emerging imaging technology applied to biological materials, offers a number of significa...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Liang X,Graf BW,Boppart SA

    更新日期:2009-11-01 00:00:00

  • Dynamic imaging and quantification of subcellular motion with eigen-decomposition optical coherence tomography-based variance analysis.

    abstract::The dynamic properties of subcellular organism are important biomarkers of the health. Imaging subcellular level dynamics provides effective solutions for evaluating cell metabolism and testing the responses of cells to pathogens and drugs in pharmaceutical engineering. In this paper, we demonstrate an innovative appr...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Wei W,Tang P,Xie Z,Li Y,Wang RK

    更新日期:2019-10-01 00:00:00