Abstract:
:Photodynamic inactivation (PDI) is a promising alternative for combating infections caused by antimicrobial resistant bacteria. Pneumonias are among the most worrisome infections because of their high-mortality rate. Previous studies have demonstrated the feasibility of using PDI with extracorporeal light to treat pneumonia. In this study, we analyzed key parameters for the viability of this treatment, including the selectivity of the photodynamic response for pathogens over host cells. Our results showed that PDI can induce killing of Staphylococcus aureus (of up to 4.18 log for the strain Xen29 and 3.62 log for Xen36) under conditions where little or no toxicity for host cells is observed. We validated pulmonary delivery of the photosensitizer and light in mice, using photobleaching as an indicator, and demonstrated preservation of healthy tissues as evidence of the safety of the protocol. Overall, PDI displays low toxicity on host tissues, making it a promising tool for treatment of pneumonias caused by S. aureus and other important pathogens.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Kassab G,Cheburkanov V,Willis J,Moule MG,Kurachi C,Yakovlev V,Cirillo JD,Bagnato VSdoi
10.1002/jbio.202000176subject
Has Abstractpub_date
2020-10-01 00:00:00pages
e202000176issue
10eissn
1864-063Xissn
1864-0648journal_volume
13pub_type
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