Quantitative analysis of shape and volume changes in activated thrombocytes in real time by single-shot spatial light modulator-based differential interference contrast imaging.

Abstract:

:We suggest to use a combination of optical tweezers and single-image quantitative differential interference contrast (DIC) emulated by a spatial light modulator (SLM) to study physiological shape changes in thrombocytes after activation and demonstrate the effectiveness of this system for the given task. A specially designed phase mask displayed at the SLM enables quantitative phase calculation from only a single recording. The optical tweezers stabilize trapped thrombocytes for long-time monitoring of changes in the optical thickness profile of thrombocytes during activation by adenosine diphosphate (ADP).

journal_name

J Biophotonics

journal_title

Journal of biophotonics

authors

Khan S,Jesacher A,Nussbaumer W,Bernet S,Ritsch-Marte M

doi

10.1002/jbio.201100010

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

600-9

issue

9

eissn

1864-063X

issn

1864-0648

journal_volume

4

pub_type

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