Improving the image quality in STED nanoscopy using frequency spectrum modulation.

Abstract:

:Since stimulated emission depletion (STED) nanoscopy was invented in 1994, this technique has been widely used in the fields of biomedicine and materials science. According to the imaging principle of STED technology, increasing the power of the depletion laser within a certain threshold can improve the resolution. However, it will cause not only severe photo-damage to the samples and photo-bleaching to the fluorophores but also serious background noise, leading to the degeneration of the quality of STED images. Here we propose a new processing method based on frequency spectrum modulation to improve the quality of STED images, abbreviated as FM-STED. We have demonstrated the performance of FM-STED in improving the signal-to-noise ratio and the resolution using fluorescent beads and biological cells as samples.

journal_name

J Biophotonics

journal_title

Journal of biophotonics

authors

Wang J,Wang L,Zhang J,Yang Z,Yan W,Qu J

doi

10.1002/jbio.202000402

subject

Has Abstract

pub_date

2020-12-13 00:00:00

pages

e202000402

eissn

1864-063X

issn

1864-0648

pub_type

信件
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