Automatic and label-free identification of blood vessels in gliomas using the combination of multiphoton microscopy and image analysis.

abstract：:Human immunodeficiency virus (HIV-1) infection remains a major health problem despite the use of highly active antiretroviral therapy (HAART), which has greatly reduced mortality rates. Due to the unavailability of an effective vaccine and treatment that would completely eradicate the virus in infected individuals, th...

journal_title：Journal of biophotonics

authors： Lugongolo MY,Manoto SL,Ombinda-Lemboumba S,Maaza M,Mthunzi-Kufa P

更新日期：2017-10-01 00:00:00

abstract：:There has been increasing use of in vitro cell culture models that more realistically replicate the three-dimensional (3D) environment found in vivo. Multicellular tumor spheroids (MTS) using cell lines or patient-derived organoids have become an important in vitro drug development tool, where cells are grown in a 3D ...

journal_title：Journal of biophotonics

authors： Jamieson LE,Harrison DJ,Campbell CJ

更新日期：2019-05-01 00:00:00

abstract：:The paper presents a new algorithm based on some selected automatic quantitative methods for analysing thermal images. It shows the practical implementation of these image analysis methods in Matlab. It enables to perform fully automated and reproducible measurements of selected parameters in thermal images. The paper...

journal_title：Journal of biophotonics

authors： Koprowski R

更新日期：2016-05-01 00:00:00

abstract：:In fluctuation-based optical nanoscopy, investigating high-density labeled subcellular structures with high fidelity has been a significant challenge. In this study, based on super-resolution radial fluctuation (SRRF) microscopy, the joint tagging (JT) strategy is employed to enable fast high-density nanoscopic imagin...

journal_title：Journal of biophotonics

authors： Zeng Z,Ma J,Xi P,Xu C

更新日期：2018-09-01 00:00:00

abstract：:Surface-enhanced Raman scattering (SERS) is highly sensitive and label-free analytical technique based on Raman spectroscopy aided by field-multiplying plasmonic nanostructures. We report the use of SERS measurements of patient urine in conjunction with biostatistical algorithms to assess the treatment response of pro...

journal_title：Journal of biophotonics

authors： Ma Y,Chi J,Zheng Z,Attygalle A,Kim IY,Du H

更新日期：2021-01-01 00:00:00

abstract：:Mucosal lesions of the upper aerodigestive tract usually concern the squamous epithelium of the surface. Many precancerous alterations now designated as potentially malignant are caused by DNA damage resulting from long term exposure to tobacco and alcohol consumption. The distinction from conditions with a similar ph...

journal_title：Journal of biophotonics

authors： Dreyer T,Kreisel M

更新日期：2012-04-01 00:00:00

abstract：:Atherosclerosis (AS), the leading cause of morbidity and mortality in cardiovascular disease, needs an early detection for treatment and prevention of fatal events. Here, for the first time, we applied gold nanorods (GNRs)-assisted diffusion reflection (DR), a noninvasive technique for in vivo detection of AS in a hig...

journal_title：Journal of biophotonics

authors： Chakraborty R,Ankri R,Leshem-Lev D,Hochhauser E,Kornowski R,Motiei M,Lev EI,Fixler D

更新日期：2019-01-01 00:00:00

abstract：:Photodynamic inactivation of bacteria (PDIB) is considered a new approach for the struggle against multiresistant bacteria. To achieve a sufficient level of bacteria killing, the photosensitizer must attach to and/or penetrate the bacteria and generate a sufficiently high amount of singlet oxygen. To optimize PDIB, th...

journal_title：Journal of biophotonics

authors： Regensburger J,Maisch T,Felgenträger A,Santarelli F,Bäumler W

更新日期：2010-06-01 00:00:00

abstract：:Nacre is a complex biomaterial made of aragonite-tablet bricks and organic mortar that is considerably resilient against breakage. Nacre has been studied with a wide range of laboratory techniques, leading to understanding key fundamentals and informing the creation of bio-inspired materials. In this article, we prese...

journal_title：Journal of biophotonics

authors： Jones JA,Metzler RA,D'Addario AJ,Burgess C,Regan B,Spano S,Cvarch BA,Galvez EJ

更新日期：2018-10-01 00:00:00

abstract：:Currently, optical coherence tomography (OCT), is not capable of obtaining molecular information often crucial for identification of disease. To enable molecular imaging with OCT, we have further developed a technique that harnesses transient changes in light absorption in the sample to garner molecular information. A...

journal_title：Journal of biophotonics

authors： Carrasco-Zevallos O,Shelton RL,Kim W,Pearson J,Applegate BE

更新日期：2015-01-01 00:00:00

abstract：:We fabricated composite nanoparticles consisting of a plasmonic core (gold nanorods or gold-silver nanocages) and a hematoporphyrin-doped silica shell. The dual photodynamic and photothermal activities of such nanoparticles against Staphylococcus aureus 209 P were studied and compared with the activities of reference ...

journal_title：Journal of biophotonics

authors： Khlebtsov BN,Tuchina ES,Khanadeev VA,Panfilova EV,Petrov PO,Tuchin VV,Khlebtsov NG

更新日期：2013-04-01 00:00:00

abstract：:The effect of cetyl-trimethylammonium bromide (CTAB) on enhancing the fluorescence resonance energy transfer (FRET) between two dye-conjugated DNA strands was studied using fluorescence emission spectroscopy and dynamic light scattering (DLS). For hybridized DNA where one strand is conjugated with a TAMRA donor and th...

journal_title：Journal of biophotonics

authors： Oh T,Takahashi T,Kim S,Heller MJ

更新日期：2016-01-01 00:00:00

abstract：:Photoacoustic imaging (PAI) is a hybrid imaging modality with high resolution and sensitivity that can be beneficial for cancer staging. Due to insufficient endogenous photoacoustic (PA) contrast, the development of exogenous agents is critical in targeting cancerous tumors. The current study demonstrates the feasibil...

journal_title：Journal of biophotonics

authors： Nguyen VP,Park S,Oh J,Wook Kang H

更新日期：2017-08-01 00:00:00

abstract：:Optical coherence tomography (OCT) is a well-developed technology that utilizes near-infrared light to reconstruct three-dimensional images of biological tissues with micrometer resolution. Improvements of the imaging contrast of the OCT technique are able to further widen its extensive biomedical applications. In thi...

journal_title：Journal of biophotonics

authors： Zhang J,Liu J,Wang LM,Li ZY,Yuan Z

更新日期：2017-06-01 00:00:00

abstract：:The research work presented in this paper focuses on qualitative tissue differentiation by monitoring the intensity ratios of atomic emissions using 'Laser Induced Breakdown Spectroscopy' (LIBS) on the plasma plume created during laser tissue ablation. The background of this study is to establish a real time feedback ...

journal_title：Journal of biophotonics

authors： Kanawade R,Mahari F,Klämpfl F,Rohde M,Knipfer C,Tangermann-Gerk K,Adler W,Schmidt M,Stelzle F

更新日期：2015-01-01 00:00:00

abstract：:Laser tweezers Raman spectroscopy (LTRS) as a label-free and noninvasive technology has been proven to be an ideal tool for analysis of single living cells, which provides important fingerprint information without interference from surrounding environments. For the first time, LTRS system was successfully used to exam...

journal_title：Journal of biophotonics

authors： Liu M,Liu X,Huang Z,Tang X,Lin X,Xu Y,Chen G,Kwok HF,Lin Y,Feng S

更新日期：2019-03-01 00:00:00

abstract：:In this work we present how to entirely remove the scattering ambiguity present in existing multiphoton multifocal systems. This is achieved through the development and implementation of single-element detection systems that incorporate high-speed photon-counting electronics. These systems can be used to image entire ...

journal_title：Journal of biophotonics

authors： Hoover EE,Field JJ,Winters DG,Young MD,Chandler EV,Speirs JC,Lapenna JT,Kim SM,Ding SY,Bartels RA,Wang JW,Squier JA

更新日期：2012-05-01 00:00:00

abstract：:Ectasia after refractive surgery represents a major concern among refractive surgeons. Corneal abnormalities and preexisting corneal ectasia are the most important risk factors. Corneal topography and central corneal thickness are the factors traditionally screening for in refractive surgery candidates. Study of the a...

journal_title：Journal of biophotonics

authors： Luz A,Lopes B,Salomão M,Ambrósio R

更新日期：2016-05-01 00:00:00

abstract：:Methods of nonlinear optics provide a vast arsenal of tools for label-free brain imaging, offering a unique combination of chemical specificity, the ability to detect fine morphological features, and an unprecedentedly high, subdiffraction spatial resolution. While these techniques provide a rapidly growing platform f...

journal_title：Journal of biophotonics

authors： Lanin AA,Pochechuev MS,Chebotarev AS,Kelmanson IV,Belousov VV,Zheltikov AM

更新日期：2019-11-01 00:00:00

abstract：:The dynamic properties of subcellular organism are important biomarkers of the health. Imaging subcellular level dynamics provides effective solutions for evaluating cell metabolism and testing the responses of cells to pathogens and drugs in pharmaceutical engineering. In this paper, we demonstrate an innovative appr...

journal_title：Journal of biophotonics

authors： Wei W,Tang P,Xie Z,Li Y,Wang RK

更新日期：2019-10-01 00:00:00

abstract：:The use of conventional fluorescence microscopy for characterizing tissue pathological states is limited by overlapping spectra and the dependence on excitation power and fluorophore concentration. Fluorescence lifetime imaging microscopy (FLIM) can overcome these limitations due to its insensitivity to fluorophore co...

journal_title：Journal of biophotonics

authors： Gu J,Fu CY,Ng BK,Gulam Razul Ss,Lim SK

更新日期：2014-07-01 00:00:00

abstract：:Micro-/nanosphere-mediated femtosecond laser cell perforation is one of the high throughput technologies used for macro-molecule-delivery into multiple cells. We have demonstrated the delivery of plasmid-DNA/liposome complexes into cells using biodegradable polymer microspheres and a femtosecond laser and investigated...

journal_title：Journal of biophotonics

authors： Ishii A,Hiruta Y,Heinemann D,Heisterkamp A,Kanazawa H,Terakawa M

更新日期：2017-12-01 00:00:00

abstract：:We present the design and construction of a prototype imaging probe capable of co-registered pulse-echo ultrasound and photoacoustic (optoacoustic) imaging in real time. The probe consists of 36 fibers of 200 micron core diameter each that are distributed around a commercial transvaginal ultrasound transducer, and hou...

journal_title：Journal of biophotonics

authors： Kumavor PD,Alqasemi U,Tavakoli B,Li H,Yang Y,Sun X,Warych E,Zhu Q

更新日期：2013-06-01 00:00:00

abstract：:Quantification of the mucosa oxygenation levels during Endoscopic imaging provides useful physiological/diagnostic information. In this work a method for non-contact quantification of the oxygen saturation index during Endoscopic imaging using three discrete spectral-band in the blue, the green, and the red parts of t...

journal_title：Journal of biophotonics

authors： Fawzy Y

更新日期：2009-12-01 00:00:00

abstract：:A robust method is presented for evaluating the diffusion properties of chemicals in ex vivo biological tissues. Using this method that relies only on thickness and collimated transmittance measurements, the diffusion properties of glycerol, fructose, polypropylene glycol and water in muscle tissues were evaluated. Am...

journal_title：Journal of biophotonics

authors： Carneiro I,Carvalho S,Henrique R,Oliveira LM,Tuchin VV

更新日期：2019-04-01 00:00:00

abstract：:Achieving a maximal safe extent of resection during brain tumor surgery is the goal for improved patient prognosis. Fluorescence-guided neurosurgery using 5-aminolevulinic acid (5-ALA) induced protoporphyrin IX has thereby become a valuable tool enabling a high frequency of complete resections and a prolonged progress...

journal_title：Journal of biophotonics

authors： Erkkilä MT,Bauer B,Hecker-Denschlag N,Madera Medina MJ,Leitgeb RA,Unterhuber A,Gesperger J,Roetzer T,Hauger C,Drexler W,Widhalm G,Andreana M

更新日期：2019-06-01 00:00:00

abstract：:The purpose of this study was to evaluate the tomographic features of postrefractive surgery eyes. This was a retrospective evaluation of clinical data. Three patients with post-LASIK (laser-assisted in situ keratomileusis) and two patients with post-SMILE (small incision lenticule extraction) ectasia were imaged with...

journal_title：Journal of biophotonics

authors： Khamar P,Dalal R,Chandapura R,Francis M,Shetty R,Nelson EJR,Nuijts RMMA,Sinha Roy A

更新日期：2019-02-01 00:00:00

abstract：:Despite an ever increasing demand for reliable and cheap methods in the detection and quantification of microbes, surprisingly few investigations have explored or utilized the luminescence of rare earths in the microbial context, neither in conventional, that is, plating and microscopic imaging techniques, nor in adva...

journal_title：Journal of biophotonics

authors： Lezhnina MM,Rochowiak W,Göhde W,Kuczius R,Kynast U

更新日期：2020-09-01 00:00:00

abstract：:Skin fibrosis, often referred to as skin scarring, is a significant international health problem with limited treatment options. The hallmarks of skin fibrosis are increased fibroblast proliferation, collagen production, and migration speed. Recently published clinical observations indicate that visible red light may ...

journal_title：Journal of biophotonics

authors： Mamalis A,Koo E,Garcha M,Murphy WJ,Isseroff RR,Jagdeo J

更新日期：2016-12-01 00:00:00

abstract：:Lasers are used in dentistry for a variety of indications. One of these is the disinfection of root canals or the sterilization of residual caries. Many studies have demonstrated the capacity to kill bacteria for lasers but the fundamental mechanism of the laser effect remains quite unclear. With our experiments we wa...

journal_title：Journal of biophotonics

authors： Hibst R,Graser R,Udart M,Stock K

更新日期：2010-06-01 00:00:00