Abstract：
:The effect of cetyl-trimethylammonium bromide (CTAB) on enhancing the fluorescence resonance energy transfer (FRET) between two dye-conjugated DNA strands was studied using fluorescence emission spectroscopy and dynamic light scattering (DLS). For hybridized DNA where one strand is conjugated with a TAMRA donor and the other with a TexasRed acceptor, increasing the concentration of CTAB changes the fluorescence emission properties and improves the FRET transfer efficiency through changes in the polarity of the solvent, neutralization of the DNA backbone and micelle formation. For the DNA FRET system without CTAB, the DNA hybridization leads to contact quenching between TAMRA donor and TexasRed acceptor producing reduced donor emission and only a small increase in acceptor emission. At 50 µM CTAB, however, the sheathing and neutralization of the dye-conjugated dsDNA structure significantly reduces quenching by DNA bases and dye interactions, producing a large increase in FRET efficiency, which is almost four fold higher than without CTAB.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Oh T,Takahashi T,Kim S,Heller MJdoi
10.1002/jbio.201500221subject
Has Abstractpub_date
2016-01-01 00:00:00pages
49-54issue
1-2eissn
1864-063Xissn
1864-0648journal_volume
9pub_type
杂志文章abstract：:Malaria is a life-threatening infectious blood disease affecting humans and other animals caused by parasitic protozoans belonging to the Plasmodium type especially in developing countries. The gold standard method for the detection of malaria is through the microscopic method of chemically treated blood smears. We de...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201700279
更新日期：2018-05-01 00:00:00
abstract：:Articular cartilage posesses unique material properties due to a complex depth-dependent composition of sub-components. Raman spectroscopy has proven valuable in quantifying this composition through cartilage cross-sections. However, cross-sectioning requires tissue destruction and is not practical in situ. In this wo...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.202000289
更新日期：2021-01-01 00:00:00
abstract：:We introduce a unique method for laser-propelling individual cells over distances of 10s of cm through stationary liquid in a microfluidic channel. This is achieved by using liquid-filled hollow-core photonic crystal fiber (HC-PCF). HC-PCF provides low-loss light guidance in a well-defined single mode, resulting in hi...
journal_title：Journal of biophotonics
pub_type： 社论
doi：10.1002/jbio.201200180
更新日期：2013-09-01 00:00:00
abstract：:Atherosclerosis is a process of thickening and stiffening of the arterial walls through the accumulation of lipids and fibrotic material, as a consequence of aging and unhealthy life style. However, not all arterial plaques lead to complications, which can lead to life-threatening events such as stroke and myocardial ...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201800013
更新日期：2018-12-01 00:00:00
abstract：:We present a noninvasive and noncontacting biosensor using Wavelength Modulated Differential Photothermal Radiometry (WM-DPTR) to monitor blood glucose concentration (BGC) through interstitial fluid (ISF) probing in human middle fingers. WM-DPTR works in the interference-free mid-infrared range with differential wavel...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201800441
更新日期：2019-07-01 00:00:00
abstract：:Stimulated Raman scattering (SRS) has been applied to unstained samples of articular cartilage enabling the investigation of living cells within fresh tissue. Hyperspectral SRS measurements over the CH vibrational region showed variations in protein and lipid content within the cells, pericellular matrix and interterr...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201200213
更新日期：2013-10-01 00:00:00
abstract：:For a precise manipulation of particles and cells with laser light as well as for the understanding and the control of the underlying processes it is important to visualize and quantify the response of the specimens. Thus, we investigated if digital holographic microscopy (DHM) can be used in combination with microflu...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201000035
更新日期：2010-07-01 00:00:00
abstract：:Dynamic follow-up of exogenous molecules permeation through the skin is one among many competing applications for confocal Raman microspectroscopy. Previous studies showed the feasibility of tracking actives through the skin; the next step should be recording in vivo kinetics. Thus, we conducted a study to evaluate th...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201300011
更新日期：2014-09-01 00:00:00
abstract：:An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probes-upon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensors-is reported. To this end, high sensitivity photonic sensors based on silicon photonic bandgap (PBG) structur...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201800030
更新日期：2018-10-01 00:00:00
abstract：:Methods of nonlinear optics provide a vast arsenal of tools for label-free brain imaging, offering a unique combination of chemical specificity, the ability to detect fine morphological features, and an unprecedentedly high, subdiffraction spatial resolution. While these techniques provide a rapidly growing platform f...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201800432
更新日期：2019-11-01 00:00:00
abstract：:Noninvasive body shaping is becoming a growing demand. The aim of this study was to investigate the efficacy and safety of the combined treatments of 1064 nm Nd:YAG and 2940 nm Er:YAG in noninvasive lipolysis and skin tightening. Ten females were enrolled, and all women's side of the waist or the lower part of the abd...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201900083
更新日期：2019-09-01 00:00:00
abstract：:An integrated multimodal optical microscope is demonstrated for high-resolution, structural and functional imaging of engineered and natural skin. This microscope incorporates multiple imaging modalities including optical coherence (OCM), multi-photon (MPM), and fluorescence lifetime imaging microscopy (FLIM), enablin...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201200003
更新日期：2012-05-01 00:00:00
abstract：:A plastic optical fibre biosensor based on surface plasmon resonance for the detection of C-reactive protein (CRP) in serum is proposed. The biosensor was integrated into a home-made thermo-stabilized microfluidic system that allows avoiding any thermal and/or mechanical fluctuation and maintaining the best stable con...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201500315
更新日期：2016-10-01 00:00:00
abstract：:The purpose of this study was to evaluate early vascular and tomographic changes in the retina of diabetic patients using artificial intelligence (AI). The study included 74 age-matched normal eyes, 171 diabetic eyes without retinopathy (DWR) eyes and 69 mild non-proliferative diabetic retinopathy (NPDR) eyes. All pat...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.202000107
更新日期：2020-09-01 00:00:00
abstract：:Three-dimensional reconstruction of tissue structures is essential for biomedical research. The development of light microscopes and various fluorescent labeling techniques provides powerful tools for this motivation. However, optical imaging depth suffers from strong light scattering due to inherent heterogeneity of ...
journal_title：Journal of biophotonics
pub_type： 杂志文章,评审
doi：10.1002/jbio.201700187
更新日期：2018-02-01 00:00:00
abstract：:Photoacoustic microscopy (PAM) is a noninvasive imaging technique and is excellent to study structural and functional changes in the microcirculation. In this work, a lipopolysaccharide (LPS)-induced inflammation model in mice is noninvasively evaluated by PAM. PAM is used to image the microvascular structural changes...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201800251
更新日期：2019-03-01 00:00:00
abstract：:Nacre is a complex biomaterial made of aragonite-tablet bricks and organic mortar that is considerably resilient against breakage. Nacre has been studied with a wide range of laboratory techniques, leading to understanding key fundamentals and informing the creation of bio-inspired materials. In this article, we prese...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201800026
更新日期：2018-10-01 00:00:00
abstract：:Optical coherence tomography (OCT) is a well-developed technology that utilizes near-infrared light to reconstruct three-dimensional images of biological tissues with micrometer resolution. Improvements of the imaging contrast of the OCT technique are able to further widen its extensive biomedical applications. In thi...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201600047
更新日期：2017-06-01 00:00:00
abstract：:Drowsy driving contributes to ~20% of all traffic accidents worldwide. Onsite monitoring the mental condition of a driver and forewarning may be a preventive solution to reduce occurrence of drowsiness and potential accidents. Functional near-infrared spectroscopy (fNIRS) has been successfully utilized in hemodynamics...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201800160
更新日期：2018-12-01 00:00:00
abstract：:This contribution covers recent original research papers in the biophotonics field. The content is organized into main techniques such as multiphoton microscopy, Raman spectroscopy, infrared spectroscopy, optical coherence tomography and photoacoustic tomography, and their applications in the context of fluid, cell, t...
journal_title：Journal of biophotonics
pub_type： 杂志文章,评审
doi：10.1002/jbio.201600290
更新日期：2016-12-01 00:00:00
abstract：:We have investigated the ability of dual-frequency Coherent Antistokes Raman Scattering (D-CARS) micro-spectroscopy, based on femtosecond pulses (100 fs or 5 fs) spectrally focussed by glass dispersion, to distinguish the chemical composition of micron-sized lipid droplets consisting of different triglycerides types (...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201200197
更新日期：2014-01-01 00:00:00
abstract：:Surface-enhanced Raman scattering (SERS) spectroscopy possesses the most promising advantage of multiplex detection for biosensing applications, which is achieved due to the narrow 'fingerprint' Raman spectra from the analyte molecules. We developed an ultrasensitive platform for the multiplex detection of cancer biom...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201300084
更新日期：2014-11-01 00:00:00
abstract：:The investigation of lung dynamics on alveolar scale is crucial for the understanding and treatment of lung diseases, such as acute lung injury and ventilator induced lung injury, and to promote the development of protective ventilation strategies. One approach to this is the establishment of numerical simulations of ...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201200027
更新日期：2013-02-01 00:00:00
abstract：:This study presents numerical simulations of UVC light propagation through seven different filtered face respirators (FFR) to determine their suitability for Ultraviolet germicidal inactivation (UVGI). UV propagation was modeled using the FullMonte program for two external light illuminations. The optical properties o...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.202000232
更新日期：2020-12-01 00:00:00
abstract：:Atherosclerosis (AS), the leading cause of morbidity and mortality in cardiovascular disease, needs an early detection for treatment and prevention of fatal events. Here, for the first time, we applied gold nanorods (GNRs)-assisted diffusion reflection (DR), a noninvasive technique for in vivo detection of AS in a hig...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201800218
更新日期：2019-01-01 00:00:00
abstract：:Previous studies for melanin visualization in the retinal pigment epithelium (RPE) have exploited either its absorption properties (using photoacoustic tomography or photothermal optical coherence tomography [OCT]) or its depolarization properties (using polarization sensitive OCT). However, these methods are only sui...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201900153
更新日期：2019-12-01 00:00:00
abstract：:We develop a confocal system equipped with optimal elliptical apertures to improve axial point spread function and signal-to-background ratio (SBR) for different detector sizes. By adjusting the parameters of the elliptical apertures, the axial half width at half-maximum can be reduced to 4.986 (described in optical c...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201700293
更新日期：2018-05-01 00:00:00
abstract：:The paper presents a new algorithm based on some selected automatic quantitative methods for analysing thermal images. It shows the practical implementation of these image analysis methods in Matlab. It enables to perform fully automated and reproducible measurements of selected parameters in thermal images. The paper...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201500224
更新日期：2016-05-01 00:00:00
abstract：:Despite an ever increasing demand for reliable and cheap methods in the detection and quantification of microbes, surprisingly few investigations have explored or utilized the luminescence of rare earths in the microbial context, neither in conventional, that is, plating and microscopic imaging techniques, nor in adva...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.202000068
更新日期：2020-09-01 00:00:00
abstract：:The processes associated with pollen germination were studied in vitro for two tree species, Salix caprea and Fraxinus excelsior under different nutrient conditions. The results provide evidence of changes in chemical composition of the pollen grains during germination. From the comparison of spectra of the pollen gra...
journal_title：Journal of biophotonics
pub_type： 杂志文章
doi：10.1002/jbio.201000031
更新日期：2010-08-01 00:00:00
