Abstract:
:There has been increasing use of in vitro cell culture models that more realistically replicate the three-dimensional (3D) environment found in vivo. Multicellular tumor spheroids (MTS) using cell lines or patient-derived organoids have become an important in vitro drug development tool, where cells are grown in a 3D "sphere" that exhibits many of the characteristics found in vivo. Significantly, MTS develop gradients in nutrients and oxygen, commonly found in tumors that contribute to therapy resistance. While MTS show promise as a more realistic in vitro culture model, there is a massive need to improve imaging technologies for assessing biochemical characteristics and drug response in such models to maximize their translation into useful applications such as high throughput screening (HTS). In this study, we investigate the potential for Raman spectroscopy to unveil biochemical information in MTS and have investigated how spheroid age influences drug response, shedding light on increased therapy resistance in developing tumors. The wealth of molecular level information delivered by Raman spectroscopy in a noninvasive manner, could aid translation of these 3D models into HTS applications.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Jamieson LE,Harrison DJ,Campbell CJdoi
10.1002/jbio.201800201subject
Has Abstractpub_date
2019-05-01 00:00:00pages
e201800201issue
5eissn
1864-063Xissn
1864-0648journal_volume
12pub_type
杂志文章abstract::The structural organization of cellular membranes has an essential influence on their functionality. The membrane surfaces currently are considered to consist of various distinct patches, which play an important role in many processes, however, not all parameters such as size and distribution are fully determined. In ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
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journal_title:Journal of biophotonics
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abstract::Since stimulated emission depletion (STED) nanoscopy was invented in 1994, this technique has been widely used in the fields of biomedicine and materials science. According to the imaging principle of STED technology, increasing the power of the depletion laser within a certain threshold can improve the resolution. Ho...
journal_title:Journal of biophotonics
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journal_title:Journal of biophotonics
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journal_title:Journal of biophotonics
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abstract::Skin fibrosis is a chronic debilitating feature of several skin diseases that lead to characteristic increases in dermal fibroblast proliferation and collagen deposition through upregulation in components of the transforming growth factor beta (TGF-B)/SMAD pathway. In contrast to ultraviolet phototherapy, high-fluence...
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journal_title:Journal of biophotonics
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journal_title:Journal of biophotonics
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journal_title:Journal of biophotonics
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journal_title:Journal of biophotonics
pub_type: 杂志文章
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更新日期:2019-06-01 00:00:00
abstract::Photoacoustic microscopy (PAM) is a noninvasive imaging technique and is excellent to study structural and functional changes in the microcirculation. In this work, a lipopolysaccharide (LPS)-induced inflammation model in mice is noninvasively evaluated by PAM. PAM is used to image the microvascular structural changes...
journal_title:Journal of biophotonics
pub_type: 杂志文章
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journal_title:Journal of biophotonics
pub_type: 杂志文章
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journal_title:Journal of biophotonics
pub_type: 杂志文章
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journal_title:Journal of biophotonics
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abstract::Microcalcifications are an early mammographic sign of breast cancer and a target for stereotactic breast needle biopsy. Here, we develop and compare different approaches for developing Raman classification algorithms to diagnose invasive and in situ breast cancer, fibrocystic change and fibroadenoma that can be associ...
journal_title:Journal of biophotonics
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abstract::In this work we aim to show how it is possible to exploit the second harmonic generation (SHG) signal for producing multimodal microscopic images of biological tissues. SHG microscopy constitutes an important tool for high-resolution, high-contrast, three-dimensional studies of live cell and tissue architectures. The ...
journal_title:Journal of biophotonics
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abstract::The aim of this work was to assess the antimicrobial efficacy on Pseudomonas aeruginosa of nanomicelles loaded with curcumin (CUR) alone and activated by blue laser light in an antimicrobial photodynamic therapy (APDT) approach. First, free CUR in liquid suspension and loaded in three amphiphilic nanomicelles (CUR-DAP...
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