Abstract:
:The structural organization of cellular membranes has an essential influence on their functionality. The membrane surfaces currently are considered to consist of various distinct patches, which play an important role in many processes, however, not all parameters such as size and distribution are fully determined. In this study, purple membrane (PM) patches isolated from Halobacterium salinarum were investigated in a first step using TERS (tip-enhanced Raman spectroscopy). The characteristic Raman modes of the resonantly enhanced component of the purple membrane lattice, the retinal moiety of bacteriorhodopsin, were found to be suitable as PM markers. In a subsequent experiment a single Halobacterium salinarum was investigated with TERS. By means of the PM marker bands it was feasible to identify and localize PM patches on the bacterial surface. The size of these areas was determined to be a few hundred nanometers.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Deckert-Gaudig T,Böhme R,Freier E,Sebesta A,Merkendorf T,Popp J,Gerwert K,Deckert Vdoi
10.1002/jbio.201100131subject
Has Abstractpub_date
2012-07-01 00:00:00pages
582-91issue
7eissn
1864-063Xissn
1864-0648journal_volume
5pub_type
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