Abstract:
:We combined Michelson-interferometer-based off-axis digital holographic microscopy (DHM) with a common flow cytometry (FCM) arrangement. Utilizing object recognition procedures and holographic autofocusing during the numerical reconstruction of the acquired off-axis holograms, sharply focused quantitative phase images of suspended cells in flow were retrieved without labeling, from which biophysical cellular features of distinct cells, such as cell radius, refractive index and dry mass, can be subsequently retrieved in an automated manner. The performance of the proposed concept was first characterized by investigations on microspheres that were utilized as test standards. Then, we analyzed two types of pancreatic tumor cells with different morphology to further verify the applicability of the proposed method for quantitative live cell imaging. The retrieved biophysical datasets from cells in flow are found in good agreement with results from comparative investigations with previously developed DHM methods under static conditions, which demonstrates the effectiveness and reliability of our approach. Our results contribute to the establishment of DHM in imaging FCM and prospect to broaden the application spectrum of FCM by providing complementary quantitative imaging as well as additional biophysical cell parameters which are not accessible in current high-throughput FCM measurements.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Min J,Yao B,Trendafilova V,Ketelhut S,Kastl L,Greve B,Kemper Bdoi
10.1002/jbio.201900085subject
Has Abstractpub_date
2019-09-01 00:00:00pages
e201900085issue
9eissn
1864-063Xissn
1864-0648journal_volume
12pub_type
杂志文章abstract::Intraoperative detection of residual malignant cells at tumor margins following excision of primary tumors could help improving surgery and thus patients' outcome. The feasibility of the tumor antigens epidermal growth factor receptor (EGF-R) and epithelial cell adhesion molecule (EpCAM) for antibody-dependent confoca...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600238
更新日期:2017-10-01 00:00:00
abstract::For the screening purposes urine is an especially attractive biofluid, since it offers easy and noninvasive sample collection and provides a snapshot of the whole metabolic status of the organism, which may change under different pathological conditions. Raman spectroscopy (RS) has the potential to monitor these chang...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900143
更新日期:2020-01-01 00:00:00
abstract::The capabilities of cross-polarization optical coherence tomography (CP OCT) for early bladder-cancer detection are assessed in statistical study and compared with the traditional OCT. Unlike the traditional OCT that demonstrates images only in copolarization, CP OCT acquires images in cross-polarization and copolariz...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201000088
更新日期:2011-08-01 00:00:00
abstract::Photoacoustic microscopy (PAM) is a noninvasive imaging technique and is excellent to study structural and functional changes in the microcirculation. In this work, a lipopolysaccharide (LPS)-induced inflammation model in mice is noninvasively evaluated by PAM. PAM is used to image the microvascular structural changes...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800251
更新日期:2019-03-01 00:00:00
abstract::Laser tweezers Raman spectroscopy (LTRS) as a label-free and noninvasive technology has been proven to be an ideal tool for analysis of single living cells, which provides important fingerprint information without interference from surrounding environments. For the first time, LTRS system was successfully used to exam...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800332
更新日期:2019-03-01 00:00:00
abstract::This paper comments on the article "Retinal pulse wave velocity measurement using spectral-domain optical coherence tomography" by Qian Li et al. The authors propose a method to determine the pulse wave velocity in retinal arteries and veins. This method should enable a noninvasive determination of biomechanical prope...
journal_title:Journal of biophotonics
pub_type: 评论,杂志文章
doi:10.1002/jbio.201700347
更新日期:2018-02-01 00:00:00
abstract::Photobiomodulation (PBM) therapy has been noted to promote cell proliferation and growth in many different cell types shown both in vitro and in vivo. Currently, treatment regimens are used in the clinic for a variety of ailments, including wound healing. However, most protocols treat an anatomical site without consid...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600025
更新日期:2016-12-01 00:00:00
abstract::Microcalcifications are an early mammographic sign of breast cancer and a target for stereotactic breast needle biopsy. Here, we develop and compare different approaches for developing Raman classification algorithms to diagnose invasive and in situ breast cancer, fibrocystic change and fibroadenoma that can be associ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200098
更新日期:2013-04-01 00:00:00
abstract::Modified nucleoside in urine samples is one of the most common biomarkers for cancer screening. Therefore, we developed a novel detection method for modified nucleoside detection in human urine. In this work, the modified nucleoside from real cancer patient's urine samples was first separated and purified using the af...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800327
更新日期:2019-04-01 00:00:00
abstract::The purpose of this study was to evaluate the tomographic features of postrefractive surgery eyes. This was a retrospective evaluation of clinical data. Three patients with post-LASIK (laser-assisted in situ keratomileusis) and two patients with post-SMILE (small incision lenticule extraction) ectasia were imaged with...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800253
更新日期:2019-02-01 00:00:00
abstract::Whole slide imaging (WSI) has moved digital pathology closer to diagnostic practice in recent years. Due to the inherent tissue topography variability, accurate autofocusing remains a critical challenge for WSI and automated microscopy systems. The traditional focus map surveying method is limited in its ability to ac...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.202000227
更新日期:2020-12-01 00:00:00
abstract::In this review we provide a description of the basic concepts and paradigms currently constituting the foundations of adult stem cell biology, and discuss the role that live imaging techniques have in the development of the field. We focus on live imaging of hematopoietic stem cells (HSCs) as the basic biology and cli...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.200910072
更新日期:2009-11-01 00:00:00
abstract::The above article from the Journal of Biophotonics, published online on 9 January 2018 in Wiley Online Library as Early View (https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201700354), and in Volume 11, e201700354, has been retracted by agreement between the authors (Hong Zhong, Lin Lu, Yuanhao Wang, Hongxing Ya...
journal_title:Journal of biophotonics
pub_type: 杂志文章,撤回出版物
doi:10.1002/jbio.201890133
更新日期:2020-04-01 00:00:00
abstract::Basidiomycetes, that is, mushroom-type fungi, are known to produce pigments in response to environmental impacts. As antioxidants with a high level of unsaturation, these compounds can neutralize highly oxidative species. In the event of close contact with other microbes, the enzymatically controlled pigment productio...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700369
更新日期:2018-06-01 00:00:00
abstract::Osteoporosis is a major public health problem worldwide. Here, we present a quantitative multispectral photoacoustic method for the evaluation of bone pathologies which has significant advantages over pure ultrasonic or pure optical methods as it provides both molecular information and bone mechanical status. This is ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201500206
更新日期:2016-09-01 00:00:00
abstract::Mucosal lesions of the upper aerodigestive tract usually concern the squamous epithelium of the surface. Many precancerous alterations now designated as potentially malignant are caused by DNA damage resulting from long term exposure to tobacco and alcohol consumption. The distinction from conditions with a similar ph...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.201100085
更新日期:2012-04-01 00:00:00
abstract::Drowsy driving contributes to ~20% of all traffic accidents worldwide. Onsite monitoring the mental condition of a driver and forewarning may be a preventive solution to reduce occurrence of drowsiness and potential accidents. Functional near-infrared spectroscopy (fNIRS) has been successfully utilized in hemodynamics...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800160
更新日期:2018-12-01 00:00:00
abstract::In preclinical researches, bioluminescence tomography (BLT) has widely been used for tumor imaging and monitoring, imaged-guided tumor therapy, and so forth. For these biological applications, both tumor spatial location and morphology analysis are the leading problems needed to be taken into account. However, most ex...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201960218
更新日期:2020-04-01 00:00:00
abstract::Near-infrared light allows measuring tissue oxygenation. These measurements relay on oxygenation-dependent absorption spectral changes. However, the tissue scattering, which is also spectral dependent, introduces an intrinsic error. Most methods focus on the volume reflectance from a semi-infinite sample. We have prop...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700208
更新日期:2018-04-01 00:00:00
abstract::We report a compact, cost-effective tuned amplifier for frequency-selective amplification of the modulated signal in heterodyne detected nonlinear optical microscopy. Our method improved the signal to noise ratio by an order of magnitude compared to conventional lock-in detection, as demonstrated through stimulated Ra...
journal_title:Journal of biophotonics
pub_type: 社论
doi:10.1002/jbio.201200005
更新日期:2012-10-01 00:00:00
abstract::We report on transient membrane perforation of living cancer cells using plasmonic gold nanoparticles (AuNPs) enhanced single near infrared (NIR) femtosecond (fs) laser pulse. Under optimized laser energy fluence, single pulse treatment (τ = 45 fs, λ = 800 nm) resulted in 77% cell perforation efficiency and 90% cell v...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201500135
更新日期:2016-01-01 00:00:00
abstract::We report the use of ultrasound modulated optical tomography (UOT) with heterodyne parallel detection to locally sense and image blood flow deep inside a highly scattering medium. We demonstrate that the UOT signal is sensitive to the speed of the blood flow in the ultrasound focus and present an analytical model that...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700013
更新日期:2018-01-01 00:00:00
abstract::Hyperspectral imaging is a promising technique for resection margin assessment during cancer surgery. Thereby, only a specific amount of the tissue below the resection surface, the clinically defined margin width, should be assessed. Since the imaging depth of hyperspectral imaging varies with wavelength and tissue co...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900086
更新日期:2019-11-01 00:00:00
abstract::A conceptually new type of corneal inlays for a customized treatment of presbyopia is presented. The diffractive inlay consists on a small aperture disc having an array of micro-holes distributed inside the open zones of a Fresnel zone plate. In this way, the central hole of the disc lets pass the zero order diffracti...
journal_title:Journal of biophotonics
pub_type: 信件
doi:10.1002/jbio.201600320
更新日期:2017-09-01 00:00:00
abstract::In vivo visualization of the human lymphatic system is limited by the mode of delivery of tracing agents, depth of field and size of the area examined, and specificity of the cell markers used to distinguish lymphatic endothelium from the blood vessels and the surrounding tissues. These limitations are particularly pr...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.201700117
更新日期:2018-08-01 00:00:00
abstract::A multiple exposure laser speckle contrast imaging (MELSCI) setup for visualizing blood perfusion was developed using a field programmable gate array (FPGA), connected to a 1000 frames per second (fps) 1-megapixel camera sensor. Multiple exposure time images at 1, 2, 4, 8, 16, 32 and 64 milliseconds were calculated by...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700069
更新日期:2018-02-01 00:00:00
abstract::Stimulated Raman scattering (SRS) has been applied to unstained samples of articular cartilage enabling the investigation of living cells within fresh tissue. Hyperspectral SRS measurements over the CH vibrational region showed variations in protein and lipid content within the cells, pericellular matrix and interterr...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200213
更新日期:2013-10-01 00:00:00
abstract::A Monte Carlo simulation was adapted to specify a skin model with pigmented epidermis, dermis with low baseline blood content, and vessels of a vascular lesion with an average added blood volume fraction of 5%. In the study, the lesion vessel diameters were increased and the number of lesion vessels decreased, such th...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.200900085
更新日期:2010-01-01 00:00:00
abstract::We fabricated composite nanoparticles consisting of a plasmonic core (gold nanorods or gold-silver nanocages) and a hematoporphyrin-doped silica shell. The dual photodynamic and photothermal activities of such nanoparticles against Staphylococcus aureus 209 P were studied and compared with the activities of reference ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200079
更新日期:2013-04-01 00:00:00
abstract::The ability to provide quantitative, objective and automated pathological analysis would provide enormous benefits for national cancer screening programmes, in terms of both resource reduction and improved patient wellbeing. The move towards molecular pathology through spectroscopic methods shows great promise, but ha...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700244
更新日期:2018-04-01 00:00:00