Towards automated cancer screening: Label-free classification of fixed cell samples using wavelength modulated Raman spectroscopy.

abstract：:Biophotonics is a highly interdisciplinary field where physicists, chemists, biologists, physicians and engineers work together to solve the problems appearing in biology and medicine. In China, the Biophotonics discipline is often referred to as Biomedical Photonics, under the first-level disciplines Biomedical Engin...

journal_title：Journal of biophotonics

authors： Shi H,Luo Q

更新日期：2017-12-01 00:00:00

abstract：:For a precise manipulation of particles and cells with laser light as well as for the understanding and the control of the underlying processes it is important to visualize and quantify the response of the specimens. Thus, we investigated if digital holographic microscopy (DHM) can be used in combination with microflu...

journal_title：Journal of biophotonics

authors： Kemper B,Langehanenberg P,Höink A,von Bally G,Wottowah F,Schinkinger S,Guck J,Käs J,Bredebusch I,Schnekenburger J,Schütze K

更新日期：2010-07-01 00:00:00

abstract：:In this work we present how to entirely remove the scattering ambiguity present in existing multiphoton multifocal systems. This is achieved through the development and implementation of single-element detection systems that incorporate high-speed photon-counting electronics. These systems can be used to image entire ...

journal_title：Journal of biophotonics

authors： Hoover EE,Field JJ,Winters DG,Young MD,Chandler EV,Speirs JC,Lapenna JT,Kim SM,Ding SY,Bartels RA,Wang JW,Squier JA

更新日期：2012-05-01 00:00:00

abstract：:Ionizing radiation (IR) induces DNA damage and low-level laser therapy (LLLT) has been investigated to prevent or repair detrimental outcomes resulting from IR exposure. Few in vitro studies, however, explore the biological mechanisms underlying those LLLT benefits. Thus, in this work, fibroblasts and tumor cells are ...

journal_title：Journal of biophotonics

authors： Ramos Silva C,Cabral FV,de Camargo CF,Núñez SC,Mateus Yoshimura T,de Lima Luna AC,Maria DA,Ribeiro MS

更新日期：2016-12-01 00:00:00

abstract：:Whole slide imaging (WSI) has moved digital pathology closer to diagnostic practice in recent years. Due to the inherent tissue topography variability, accurate autofocusing remains a critical challenge for WSI and automated microscopy systems. The traditional focus map surveying method is limited in its ability to ac...

journal_title：Journal of biophotonics

authors： Bian Z,Guo C,Jiang S,Zhu J,Wang R,Song P,Zhang Z,Hoshino K,Zheng G

更新日期：2020-12-01 00:00:00

abstract：:The dynamic properties of subcellular organism are important biomarkers of the health. Imaging subcellular level dynamics provides effective solutions for evaluating cell metabolism and testing the responses of cells to pathogens and drugs in pharmaceutical engineering. In this paper, we demonstrate an innovative appr...

journal_title：Journal of biophotonics

authors： Wei W,Tang P,Xie Z,Li Y,Wang RK

更新日期：2019-10-01 00:00:00

abstract：:Skin optical clearing has shown tremendous potential in improving various optical imaging performances, but there is some certain blindness in screening out high-efficiency in vivo optical clearing methods. In this work, three optical clearing agents: sucrose (Suc), fructose (Fruc) and PEG-400 (PEG), and two chemical ...

journal_title：Journal of biophotonics

authors： Shi R,Guo L,Zhang C,Feng W,Li P,Ding Z,Zhu D

更新日期：2017-06-01 00:00:00

abstract：:The effect of cetyl-trimethylammonium bromide (CTAB) on enhancing the fluorescence resonance energy transfer (FRET) between two dye-conjugated DNA strands was studied using fluorescence emission spectroscopy and dynamic light scattering (DLS). For hybridized DNA where one strand is conjugated with a TAMRA donor and th...

journal_title：Journal of biophotonics

authors： Oh T,Takahashi T,Kim S,Heller MJ

更新日期：2016-01-01 00:00:00

abstract：:The broad range of applications of spatially-offset Raman spectroscopy (SORS) were found to involve samples having only marginal differences in Raman cross-sections between the surface and subsurface targets. We report the results of a feasibility study to evaluate the potential of the approach to identify the presenc...

journal_title：Journal of biophotonics

authors： Khan KM,Dutta SB,Krishna H,Majumder SK

更新日期：2016-09-01 00:00:00

abstract：:Monitoring living cells in real-time is important in order to unravel complex dynamic processes in life sciences. In particular the dynamics of initiation and progression of degenerative diseases is intensely studied. In atherosclerosis the thickening of arterial walls is related to high lipid levels in the blood stre...

journal_title：Journal of biophotonics

authors： Stiebing C,Meyer T,Rimke I,Matthäus C,Schmitt M,Lorkowski S,Popp J

更新日期：2017-09-01 00:00:00

abstract：:Uncoated and poly(styrene sulphonate) (PSS)-coated gold nanorods were taken up by NG108-15 neuronal cells. Exposure to 780 nm laser light at the plasmon resonance wavelength of the gold nanorods was found to induce intracellular Ca(2+) transients. The higher Ca(2+) peaks were observed at lower laser doses, with the hi...

journal_title：Journal of biophotonics

authors： Paviolo C,Haycock JW,Cadusch PJ,McArthur SL,Stoddart PR

更新日期：2014-10-01 00:00:00

abstract：:The capabilities of cross-polarization optical coherence tomography (CP OCT) for early bladder-cancer detection are assessed in statistical study and compared with the traditional OCT. Unlike the traditional OCT that demonstrates images only in copolarization, CP OCT acquires images in cross-polarization and copolariz...

journal_title：Journal of biophotonics

authors： Gladkova N,Streltsova O,Zagaynova E,Kiseleva E,Gelikonov V,Gelikonov G,Karabut M,Yunusova K,Evdokimova O

更新日期：2011-08-01 00:00:00

abstract：:This paper comments on the article "Retinal pulse wave velocity measurement using spectral-domain optical coherence tomography" by Qian Li et al. The authors propose a method to determine the pulse wave velocity in retinal arteries and veins. This method should enable a noninvasive determination of biomechanical prope...

journal_title：Journal of biophotonics

authors： Spahr H,Hillmann D,Pfäffle C,Hüttmann G

更新日期：2018-02-01 00:00:00

abstract：:Laser tweezers Raman spectroscopy (LTRS) as a label-free and noninvasive technology has been proven to be an ideal tool for analysis of single living cells, which provides important fingerprint information without interference from surrounding environments. For the first time, LTRS system was successfully used to exam...

journal_title：Journal of biophotonics

authors： Liu M,Liu X,Huang Z,Tang X,Lin X,Xu Y,Chen G,Kwok HF,Lin Y,Feng S

更新日期：2019-03-01 00:00:00

abstract：:We report a compact, cost-effective tuned amplifier for frequency-selective amplification of the modulated signal in heterodyne detected nonlinear optical microscopy. Our method improved the signal to noise ratio by an order of magnitude compared to conventional lock-in detection, as demonstrated through stimulated Ra...

journal_title：Journal of biophotonics

authors： Slipchenko MN,Oglesbee RA,Zhang D,Wu W,Cheng JX

更新日期：2012-10-01 00:00:00

abstract：:Microfluidics and photonics come together to form a field commonly referred to as 'optofluidics'. Flow cytometry provides the field with a technology base from which both microfluidic and photonic components be developed and integrated into a useful device. This article reviews some of the more recent developments to ...

journal_title：Journal of biophotonics

authors： Godin J,Chen CH,Cho SH,Qiao W,Tsai F,Lo YH

更新日期：2008-10-01 00:00:00

abstract：:Articular cartilage posesses unique material properties due to a complex depth-dependent composition of sub-components. Raman spectroscopy has proven valuable in quantifying this composition through cartilage cross-sections. However, cross-sectioning requires tissue destruction and is not practical in situ. In this wo...

journal_title：Journal of biophotonics

authors： Mason D,Murugkar S,Speirs AD

更新日期：2021-01-01 00:00:00

abstract：:Drowsy driving contributes to ~20% of all traffic accidents worldwide. Onsite monitoring the mental condition of a driver and forewarning may be a preventive solution to reduce occurrence of drowsiness and potential accidents. Functional near-infrared spectroscopy (fNIRS) has been successfully utilized in hemodynamics...

journal_title：Journal of biophotonics

authors： Li T,Lin Y,Gao Y,Zhong F

更新日期：2018-12-01 00:00:00

abstract：:Initially, it has been shown that the phase thickness and refractivity (the latter interpreted as the difference of the refractivity indices of an object and surrounding medium) depend on the functional state of mitochondria. The refractivity of various objects decreased in response to energy depletion. This dependenc...

journal_title：Journal of biophotonics

authors： Tychinsky V

更新日期：2009-09-01 00:00:00

abstract：:Acridine orange (AO) staining is used to diagnose DNA fragmentation status in sperm cells. Interferometric phase microscopy (IPM) is an optical imaging method based on digital holographic microscopy that provides quantitative morphological and refractive index imaging of cells in vitro without the need for staining. W...

journal_title：Journal of biophotonics

authors： Barnea I,Karako L,Mirsky SK,Levi M,Balberg M,Shaked NT

更新日期：2018-11-01 00:00:00

abstract：:Nacre is a complex biomaterial made of aragonite-tablet bricks and organic mortar that is considerably resilient against breakage. Nacre has been studied with a wide range of laboratory techniques, leading to understanding key fundamentals and informing the creation of bio-inspired materials. In this article, we prese...

journal_title：Journal of biophotonics

authors： Jones JA,Metzler RA,D'Addario AJ,Burgess C,Regan B,Spano S,Cvarch BA,Galvez EJ

更新日期：2018-10-01 00:00:00

abstract：:Endoscopy has been widely used in biomedical imaging and integrated with various optical and acoustic imaging modalities. Photoacoustic imaging (PAI), one of the fastest growing biomedical imaging modalities, is a noninvasive and nonionizing method that owns rich optical contrast, deep acoustic penetration depth, mult...

journal_title：Journal of biophotonics

authors： Guo H,Li Y,Qi W,Xi L

更新日期：2020-12-01 00:00:00

abstract：:Surface-enhanced Raman scattering (SERS) is highly sensitive and label-free analytical technique based on Raman spectroscopy aided by field-multiplying plasmonic nanostructures. We report the use of SERS measurements of patient urine in conjunction with biostatistical algorithms to assess the treatment response of pro...

journal_title：Journal of biophotonics

authors： Ma Y,Chi J,Zheng Z,Attygalle A,Kim IY,Du H

更新日期：2021-01-01 00:00:00

abstract：:In preclinical researches, bioluminescence tomography (BLT) has widely been used for tumor imaging and monitoring, imaged-guided tumor therapy, and so forth. For these biological applications, both tumor spatial location and morphology analysis are the leading problems needed to be taken into account. However, most ex...

journal_title：Journal of biophotonics

authors： Guo H,Gao L,Yu J,He X,Wang H,Zheng J,Yang X

更新日期：2020-04-01 00:00:00

abstract：:The purpose of this study was to evaluate early vascular and tomographic changes in the retina of diabetic patients using artificial intelligence (AI). The study included 74 age-matched normal eyes, 171 diabetic eyes without retinopathy (DWR) eyes and 69 mild non-proliferative diabetic retinopathy (NPDR) eyes. All pat...

journal_title：Journal of biophotonics

authors： Govindaswamy N,Ratra D,Dalan D,Doralli S,Tirumalai AA,Nagarajan R,Mochi T,Shetty N,Sinha Roy A

更新日期：2020-09-01 00:00:00

abstract：:FTIR microspectroscopy was applied for studying macromolecular changes in human serum samples from patients with healthy livers, and those diagnosed with liver cirrhosis or hepatocellular carcinoma (HCC). Our study demonstrated that the serum samples from HCC and cirrhotic patients could readily be discriminated from ...

journal_title：Journal of biophotonics

authors： Thumanu K,Sangrajrang S,Khuhaprema T,Kalalak A,Tanthanuch W,Pongpiachan S,Heraud P

更新日期：2014-04-01 00:00:00

abstract：:Delay-and-sum (DAS) is one of the most common algorithms used to construct the photoacoustic images due to its low complexity. However, it results in images with high sidelobes and low resolution. Delay-and-standard-deviation (DASD) weighting factor can improve the contrast of the images compared to DAS. However, it s...

journal_title：Journal of biophotonics

authors： Paridar R,Mozaffarzadeh M,Periyasamy V,Basij M,Mehrmohammadi M,Pramanik M,Orooji M

更新日期：2019-06-01 00:00:00

abstract：:The aim of this study is to identify changes in scattering with optical coherence tomography (OCT) and relate these measurements with mitochondrial changes during the initiation of apoptosis. Human retinal pigment epithelial cells were cultured and apoptosis was induced using 10% alcohol. Using the attenuation coeffic...

journal_title：Journal of biophotonics

authors： de Bruin DM,Broekgaarden M,van Gemert MJ,Heger M,de la Rosette JJ,Van Leeuwen TG,Faber DJ

更新日期：2016-09-01 00:00:00

abstract：:We present a noninvasive and noncontacting biosensor using Wavelength Modulated Differential Photothermal Radiometry (WM-DPTR) to monitor blood glucose concentration (BGC) through interstitial fluid (ISF) probing in human middle fingers. WM-DPTR works in the interference-free mid-infrared range with differential wavel...

journal_title：Journal of biophotonics

authors： Guo X,Zhang D,Shojaei-Asanjan K,Sivagurunathan K,Melnikov A,Song P,Mandelis A

更新日期：2019-07-01 00:00:00

abstract：:Osteoporosis is a major public health problem worldwide. Here, we present a quantitative multispectral photoacoustic method for the evaluation of bone pathologies which has significant advantages over pure ultrasonic or pure optical methods as it provides both molecular information and bone mechanical status. This is ...

journal_title：Journal of biophotonics

authors： Steinberg I,Turko N,Levi O,Gannot I,Eyal A

更新日期：2016-09-01 00:00:00