The metabolic component of cellular refractivity and its importance for optical cytometry.

Abstract:

:Initially, it has been shown that the phase thickness and refractivity (the latter interpreted as the difference of the refractivity indices of an object and surrounding medium) depend on the functional state of mitochondria. The refractivity of various objects decreased in response to energy depletion. This dependence was then demonstrated for other biological objects such as cyanobacteria, chloroplasts and human cells. This general response brought about the hypothesis of a certain "universal" factor that links the variable (or metabolic) component of refractivity with the object's functional state. However, the origin of this phenomenon remains unknown. Our hypothesis is founded on the dependence of polarization of bound water molecules and the activity of metabolic processes. Here, we show the results of measurements of metabolic component of refractivity different bio-objects (mitochondria, chloroplasts, spores, cancer cells) obtained using the Coherent Phase Microscope "Airyscan". Estimations indicated high (up to n approximately = 1.41-1.45) values for the equivalent refractive index of structured water in cells.

journal_name

J Biophotonics

journal_title

Journal of biophotonics

authors

Tychinsky V

doi

10.1002/jbio.200910042

subject

Has Abstract

pub_date

2009-09-01 00:00:00

pages

494-504

issue

8-9

eissn

1864-063X

issn

1864-0648

journal_volume

2

pub_type

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