Abstract:
:Photobiomodulation (PBM) therapy has been noted to promote cell proliferation and growth in many different cell types shown both in vitro and in vivo. Currently, treatment regimens are used in the clinic for a variety of ailments, including wound healing. However, most protocols treat an anatomical site without considering individual cell types constituting the target tissues. This study investigates the maximal dose threshold for oral keratinocyte and fibroblast cell types treated with near-infrared laser therapy. We observed keratinocytes have increased sensitivity to laser irradiances (>0.047 W/cm2 , 300 sec, 14.2 J/cm2 ) compared to the fibroblast cells (>0.057 W/cm2 , 300 sec, 15.1 J/cm2 ) (p < 0.0001). Laser treatments were noted to generate increased reactive oxygen species (ROS) levels in keratinocytes compared to fibroblasts that appeared to inversely correlate with higher basal catalase expression. To validate these observations, melatonin was used to treat keratinocytes to induce catalase activity (p < 0.0001). Increased melatonin-induced catalase levels were noted to significantly improve keratinocyte survival to phototoxic laser doses. These observations suggest that clinical laser dosing should account for differential effects of lasers on individual cell types to improve safety and clinical efficacy of PBM therapy.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Engel KW,Khan I,Arany PRdoi
10.1002/jbio.201600025subject
Has Abstractpub_date
2016-12-01 00:00:00pages
1148-1156issue
11-12eissn
1864-063Xissn
1864-0648journal_volume
9pub_type
杂志文章abstract::Laser tweezers Raman spectroscopy (LTRS) as a label-free and noninvasive technology has been proven to be an ideal tool for analysis of single living cells, which provides important fingerprint information without interference from surrounding environments. For the first time, LTRS system was successfully used to exam...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800332
更新日期:2019-03-01 00:00:00
abstract::Drowsy driving contributes to ~20% of all traffic accidents worldwide. Onsite monitoring the mental condition of a driver and forewarning may be a preventive solution to reduce occurrence of drowsiness and potential accidents. Functional near-infrared spectroscopy (fNIRS) has been successfully utilized in hemodynamics...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800160
更新日期:2018-12-01 00:00:00
abstract::The ability to provide quantitative, objective and automated pathological analysis would provide enormous benefits for national cancer screening programmes, in terms of both resource reduction and improved patient wellbeing. The move towards molecular pathology through spectroscopic methods shows great promise, but ha...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700244
更新日期:2018-04-01 00:00:00
abstract::In biomedical applications, nanoparticles have demonstrated the potential to eradicate abnormal cells in small localized pathological zones associated with cancer or infections. Here, we introduce a method for nanotechnology-based photothermal (PT) killing of whole organisms considered harmful to humans or the environ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200199
更新日期:2014-07-01 00:00:00
abstract::A Monte Carlo simulation was adapted to specify a skin model with pigmented epidermis, dermis with low baseline blood content, and vessels of a vascular lesion with an average added blood volume fraction of 5%. In the study, the lesion vessel diameters were increased and the number of lesion vessels decreased, such th...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.200900085
更新日期:2010-01-01 00:00:00
abstract::Uncoated and poly(styrene sulphonate) (PSS)-coated gold nanorods were taken up by NG108-15 neuronal cells. Exposure to 780 nm laser light at the plasmon resonance wavelength of the gold nanorods was found to induce intracellular Ca(2+) transients. The higher Ca(2+) peaks were observed at lower laser doses, with the hi...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201300043
更新日期:2014-10-01 00:00:00
abstract::223 Radium (223 Ra) is widely used in nuclear medicine to treat patients with osseous metastatic prostate cancer. In clinical practice 223 Ra cannot be imaged directly; however, gamma photons produced by its short-lived daughter nuclides can be captured by conventional gamma cameras. In this work, we show that 223 Ra ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700209
更新日期:2018-04-01 00:00:00
abstract::Microfluidics and photonics come together to form a field commonly referred to as 'optofluidics'. Flow cytometry provides the field with a technology base from which both microfluidic and photonic components be developed and integrated into a useful device. This article reviews some of the more recent developments to ...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.200810018
更新日期:2008-10-01 00:00:00
abstract::Photoacoustic imaging (PAI) is a hybrid imaging modality with high resolution and sensitivity that can be beneficial for cancer staging. Due to insufficient endogenous photoacoustic (PA) contrast, the development of exogenous agents is critical in targeting cancerous tumors. The current study demonstrates the feasibil...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600159
更新日期:2017-08-01 00:00:00
abstract::Solar radiation exposure is recognised to be a significant contributor to the development of skin cancer. Monitoring the simultaneous and consecutive mechanisms of interaction could provide a greater understanding of the process of photocarcinogenesis. This work presents an analysis of the biochemical and morphologica...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000337
更新日期:2020-10-24 00:00:00
abstract::Optical coherence tomography (OCT) is a well-developed technology that utilizes near-infrared light to reconstruct three-dimensional images of biological tissues with micrometer resolution. Improvements of the imaging contrast of the OCT technique are able to further widen its extensive biomedical applications. In thi...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600047
更新日期:2017-06-01 00:00:00
abstract::The purpose of this study was to evaluate thermal and near-infrared (NIR) reflectance imaging methods for the assessment of the activity of root caries lesions. In addition, changes in the lesion structure were monitored with polarization sensitive optical coherence tomography (PS-OCT). Artificial bovine and natural r...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201500333
更新日期:2017-03-01 00:00:00
abstract::Biophotonics is a highly interdisciplinary field where physicists, chemists, biologists, physicians and engineers work together to solve the problems appearing in biology and medicine. In China, the Biophotonics discipline is often referred to as Biomedical Photonics, under the first-level disciplines Biomedical Engin...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201790012
更新日期:2017-12-01 00:00:00
abstract::Osteocytes are the most abundant cells in bone and always the focus of bone research. They are embedded in the highly scattering mineralized bone matrix. Consequently, visualizing osteocytes deep in bone with subcellular resolution poses a major challenge for in vivo bone research. Here we overcome this challenge by d...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800360
更新日期:2019-04-01 00:00:00
abstract::The effect of cetyl-trimethylammonium bromide (CTAB) on enhancing the fluorescence resonance energy transfer (FRET) between two dye-conjugated DNA strands was studied using fluorescence emission spectroscopy and dynamic light scattering (DLS). For hybridized DNA where one strand is conjugated with a TAMRA donor and th...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201500221
更新日期:2016-01-01 00:00:00
abstract::The capabilities of cross-polarization optical coherence tomography (CP OCT) for early bladder-cancer detection are assessed in statistical study and compared with the traditional OCT. Unlike the traditional OCT that demonstrates images only in copolarization, CP OCT acquires images in cross-polarization and copolariz...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201000088
更新日期:2011-08-01 00:00:00
abstract::Clinicians need a way to rapidly and reliably test the correct functioning of near-infrared spectroscopy (NIRS)-based oximeters. Therefore, optical phantoms for quality assessment of NIRS oximeters are needed. The fabrication of such phantoms that mimic the optical properties of biological tissue in the NIR range repr...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800300
更新日期:2019-04-01 00:00:00
abstract::In this paper we introduce a custom scanning near-field optical microscope (SNOM) that simultaneously collects reflection and transmission near-field images along with topography. This dual-optical SNOM uses a bent probe, which allows for axial reflection imaging, accurate surface scanning, and easy identification of ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201000022
更新日期:2011-03-01 00:00:00
abstract::There has been increasing use of in vitro cell culture models that more realistically replicate the three-dimensional (3D) environment found in vivo. Multicellular tumor spheroids (MTS) using cell lines or patient-derived organoids have become an important in vitro drug development tool, where cells are grown in a 3D ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800201
更新日期:2019-05-01 00:00:00
abstract::Quantitative phase imaging (QPI) is an emerging modality, which enables the identification of abnormalities in tissue based on optical properties. QPI can be applied to any biological specimen due to its label-free imaging capability, but its use in stained tissue is unclear. Here, we study the variability of QPI with...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700402
更新日期:2018-08-01 00:00:00
abstract::Tumor metastasis to lymph nodes is an important contributory factor for cancer-related deaths despite recent developments in cancer therapy. In this study, we demonstrate that tumor in the proper axillary lymph node (PALN) of the mouse can be treated by the application of external laser light to trigger the unloading ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600241
更新日期:2017-12-01 00:00:00
abstract::In preclinical researches, bioluminescence tomography (BLT) has widely been used for tumor imaging and monitoring, imaged-guided tumor therapy, and so forth. For these biological applications, both tumor spatial location and morphology analysis are the leading problems needed to be taken into account. However, most ex...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201960218
更新日期:2020-04-01 00:00:00
abstract::Bone-marrow (BM)-derived cells have been shown to be capable of aiding skin regeneration in vivo by differentiating into keratinocytes. However, the conditions under which this occurs are not fully understood. Characterizing innate mechanisms of skin regeneration by stem cells in vivo is important for the area of stem...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200240
更新日期:2014-01-01 00:00:00
abstract::We present a novel silicon photonic biosensor using phase-shifted Bragg gratings in a slot waveguide. The optical field is concentrated inside the slot region, leading to efficient light-matter interaction. The Bragg gratings are formed with sidewall corrugations on the outside of the waveguide, and a phase shift is i...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201300012
更新日期:2013-10-01 00:00:00
abstract::Pancreatic islet isolation from donor pancreases is an essential step for the transplantation of insulin-secreting β-cells as a therapy to treat type 1 diabetes mellitus. This process however damages islet basement membranes, which can lead to islet dysfunction or death. Posttransplantation, islets are further stresse...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000375
更新日期:2020-12-01 00:00:00
abstract::In the current clinical care practice, Gleason grading system is one of the most powerful prognostic predictors for prostate cancer (PCa). The grading system is based on the architectural pattern of cancerous epithelium in histological images. However, the standard procedure of histological examination often involves ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900203
更新日期:2020-02-01 00:00:00
abstract::Currently, the targeted treatment of tumor based on the tumor microenvironment is newly developed. Blood vessels are the key parts in the tumor microenvironment, which is taken as a new visible target for tumor therapy. Multiphoton microscopy (MPM), based on the second harmonic generation and two-photon excited fluore...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900006
更新日期:2019-09-01 00:00:00
abstract::Total internal reflection fluorescence excitation (TIRF) microscopy allows the selective observation of fluorescent molecules in immediate proximity to an interface between different refractive indices. Objective-type or prism-less TIRF excitation is typically achieved with laser light sources. We here propose a simpl...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900033
更新日期:2019-11-01 00:00:00
abstract::Low-level laser therapy (LLLT) is known to enhance mitochondrial electron transfer and ATP production; thus, this study asked whether LLLT could stimulate the oxidative burst in human neutrophils (PMN) and improve their ability to kill microorganisms. Blood from healthy human subjects was collected and PMN were isolat...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600035
更新日期:2016-12-01 00:00:00
abstract::The possibilities of using Mueller matrix (MM) imaging polarimetry to assess meat quality have not yet been sufficiently explored. In the current study, the fresh porcine muscles are imaged at room temperature with a wide-field MM imaging polarimeter over 26 hours to visualize dynamics of tissue optical properties thr...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000376
更新日期:2020-11-21 00:00:00