Fluorescence lifetime metabolic mapping of hypoxia-induced damage in pancreatic pseudo-islets.

abstract：:Molecular motors have been extensively studied over the past 25 years using a range of optical microscopy methods. The specific example of RNA polymerase, responsible for transcribing the information encoded in DNA into RNA, illustrates the strength of the convergence between these recent approaches and the vast store...

journal_title：Journal of biophotonics

authors： Strick TR

更新日期：2008-09-01 00:00:00

abstract：:Osteoporosis is a major public health problem worldwide. Here, we present a quantitative multispectral photoacoustic method for the evaluation of bone pathologies which has significant advantages over pure ultrasonic or pure optical methods as it provides both molecular information and bone mechanical status. This is ...

journal_title：Journal of biophotonics

authors： Steinberg I,Turko N,Levi O,Gannot I,Eyal A

更新日期：2016-09-01 00:00:00

abstract：:Single-molecule localisation based super-resolution fluorescence imaging produces maps of the coordinates of fluorescent molecules in a region of interest. Cluster analysis algorithms provide information concerning the clustering characteristics of these molecules, often through the generation of cluster heat maps bas...

journal_title：Journal of biophotonics

authors： Griffié J,Boelen L,Burn G,Cope AP,Owen DM

更新日期：2015-11-01 00:00:00

abstract：:Retinoblastoma (RB) is a rare form of cancer of the retina most prevalent in young children. We successfully show that laser-induced cell disruption, mediated by gold plasmonic nanoparticle (NP), is a potential and efficient therapy to kill the cancerous cells. The proof of concept is demonstrated in vitro on cultured...

journal_title：Journal of biophotonics

authors： Darviot C,Hardy P,Meunier M

更新日期：2019-11-01 00:00:00

abstract：:We have investigated the ability of dual-frequency Coherent Antistokes Raman Scattering (D-CARS) micro-spectroscopy, based on femtosecond pulses (100 fs or 5 fs) spectrally focussed by glass dispersion, to distinguish the chemical composition of micron-sized lipid droplets consisting of different triglycerides types (...

journal_title：Journal of biophotonics

authors： Di Napoli C,Masia F,Pope I,Otto C,Langbein W,Borri P

更新日期：2014-01-01 00:00:00

abstract：:Intraoperative detection of residual malignant cells at tumor margins following excision of primary tumors could help improving surgery and thus patients' outcome. The feasibility of the tumor antigens epidermal growth factor receptor (EGF-R) and epithelial cell adhesion molecule (EpCAM) for antibody-dependent confoca...

journal_title：Journal of biophotonics

authors： Englhard AS,Palaras A,Volgger V,Stepp H,Mack B,Libl D,Gires O,Betz CS

更新日期：2017-10-01 00:00:00

abstract：:Photoacoustic microscopy (PAM) provides a fundamentally new tool for a broad range of studies of biological structures and functions. However, the use of PAM has been largely limited to small vertebrates due to the large size/weight and the inconvenience of the equipment. Here, we describe a portable optical-resolutio...

journal_title：Journal of biophotonics

authors： Jin T,Guo H,Yao L,Xie H,Jiang H,Xi L

更新日期：2018-04-01 00:00:00

abstract：:Despite an ever increasing demand for reliable and cheap methods in the detection and quantification of microbes, surprisingly few investigations have explored or utilized the luminescence of rare earths in the microbial context, neither in conventional, that is, plating and microscopic imaging techniques, nor in adva...

journal_title：Journal of biophotonics

authors： Lezhnina MM,Rochowiak W,Göhde W,Kuczius R,Kynast U

更新日期：2020-09-01 00:00:00

abstract：:Conventional histopathology is rapidly shifting towards digital integration. Will microscopes (and pathologists) soon be obsolete? Or are we dealing with just another image modality that leaves the core of tissue diagnosis intact? This article provides an overview of current digital pathology applications and research...

journal_title：Journal of biophotonics

authors： Brachtel E,Yagi Y

更新日期：2012-04-01 00:00:00

abstract：:Ectasia after refractive surgery represents a major concern among refractive surgeons. Corneal abnormalities and preexisting corneal ectasia are the most important risk factors. Corneal topography and central corneal thickness are the factors traditionally screening for in refractive surgery candidates. Study of the a...

journal_title：Journal of biophotonics

authors： Luz A,Lopes B,Salomão M,Ambrósio R

更新日期：2016-05-01 00:00:00

abstract：:We develop a confocal system equipped with optimal elliptical apertures to improve axial point spread function and signal-to-background ratio (SBR) for different detector sizes. By adjusting the parameters of the elliptical apertures, the axial half width at half-maximum can be reduced to 4.986 (described in optical c...

journal_title：Journal of biophotonics

authors： Tang H,Wu C,Gong W,Zheng Y,Zhu X,Wang J,Si K

更新日期：2018-05-01 00:00:00

abstract：:Tumor metastasis to lymph nodes is an important contributory factor for cancer-related deaths despite recent developments in cancer therapy. In this study, we demonstrate that tumor in the proper axillary lymph node (PALN) of the mouse can be treated by the application of external laser light to trigger the unloading ...

journal_title：Journal of biophotonics

authors： Matsuki D,Adewale O,Horie S,Okajima J,Komiya A,Oluwafemi O,Maruyama S,Mori S,Kodama T

更新日期：2017-12-01 00:00:00

abstract：:In vivo visualization of the human lymphatic system is limited by the mode of delivery of tracing agents, depth of field and size of the area examined, and specificity of the cell markers used to distinguish lymphatic endothelium from the blood vessels and the surrounding tissues. These limitations are particularly pr...

journal_title：Journal of biophotonics

authors： Lokmic Z

更新日期：2018-08-01 00:00:00

abstract：:Low-power laser irradiation (LPLI) has been extensively employed to modulate inflammation in vitro and in vivo. Previous reports from our group indicated that LPLI might regulate glycemia in diabetic animals. Diabetes results in chronic hyperglycemia and therefore chronic inflammation by upregulation of inflammatory m...

journal_title：Journal of biophotonics

authors： Fukuoka CY,Torres Schröter G,Nicolau J,Simões A

更新日期：2016-12-01 00:00:00

abstract：:We report the use of ultrasound modulated optical tomography (UOT) with heterodyne parallel detection to locally sense and image blood flow deep inside a highly scattering medium. We demonstrate that the UOT signal is sensitive to the speed of the blood flow in the ultrasound focus and present an analytical model that...

journal_title：Journal of biophotonics

authors： Hussain A,Steenbergen W,Vellekoop IM

更新日期：2018-01-01 00:00:00

abstract：:223 Radium (223 Ra) is widely used in nuclear medicine to treat patients with osseous metastatic prostate cancer. In clinical practice 223 Ra cannot be imaged directly; however, gamma photons produced by its short-lived daughter nuclides can be captured by conventional gamma cameras. In this work, we show that 223 Ra ...

journal_title：Journal of biophotonics

authors： Boschi F,De Sanctis F,Spinelli AE

更新日期：2018-04-01 00:00:00

abstract：:We present a novel silicon photonic biosensor using phase-shifted Bragg gratings in a slot waveguide. The optical field is concentrated inside the slot region, leading to efficient light-matter interaction. The Bragg gratings are formed with sidewall corrugations on the outside of the waveguide, and a phase shift is i...

journal_title：Journal of biophotonics

authors： Wang X,Flueckiger J,Schmidt S,Grist S,Fard ST,Kirk J,Doerfler M,Cheung KC,Ratner DM,Chrostowski L

更新日期：2013-10-01 00:00:00

abstract：:FTIR microspectroscopy was applied for studying macromolecular changes in human serum samples from patients with healthy livers, and those diagnosed with liver cirrhosis or hepatocellular carcinoma (HCC). Our study demonstrated that the serum samples from HCC and cirrhotic patients could readily be discriminated from ...

journal_title：Journal of biophotonics

authors： Thumanu K,Sangrajrang S,Khuhaprema T,Kalalak A,Tanthanuch W,Pongpiachan S,Heraud P

更新日期：2014-04-01 00:00:00

abstract：:Solar radiation exposure is recognised to be a significant contributor to the development of skin cancer. Monitoring the simultaneous and consecutive mechanisms of interaction could provide a greater understanding of the process of photocarcinogenesis. This work presents an analysis of the biochemical and morphologica...

journal_title：Journal of biophotonics

authors： Lopez-Gonzalez U,Casey A,J Byrne H

更新日期：2020-10-24 00:00:00

abstract：:Currently, optical coherence tomography (OCT), is not capable of obtaining molecular information often crucial for identification of disease. To enable molecular imaging with OCT, we have further developed a technique that harnesses transient changes in light absorption in the sample to garner molecular information. A...

journal_title：Journal of biophotonics

authors： Carrasco-Zevallos O,Shelton RL,Kim W,Pearson J,Applegate BE

更新日期：2015-01-01 00:00:00

abstract：:Mucosal lesions of the upper aerodigestive tract usually concern the squamous epithelium of the surface. Many precancerous alterations now designated as potentially malignant are caused by DNA damage resulting from long term exposure to tobacco and alcohol consumption. The distinction from conditions with a similar ph...

journal_title：Journal of biophotonics

authors： Dreyer T,Kreisel M

更新日期：2012-04-01 00:00:00

abstract：:One pertinent complication in bacterial infection is the growth of biofilms, that is, communities of surface-adhered bacteria resilient to antibiotics. Photodynamic inactivation (PDI) has been proposed as an alternative to antibiotic treatment; however, novel techniques complementing standard efficacy measures are req...

journal_title：Journal of biophotonics

authors： Thomsen H,Graf FE,Farewell A,Ericson MB

更新日期：2018-10-01 00:00:00

abstract：:Human immunodeficiency virus (HIV-1) infection remains a major health problem despite the use of highly active antiretroviral therapy (HAART), which has greatly reduced mortality rates. Due to the unavailability of an effective vaccine and treatment that would completely eradicate the virus in infected individuals, th...

journal_title：Journal of biophotonics

authors： Lugongolo MY,Manoto SL,Ombinda-Lemboumba S,Maaza M,Mthunzi-Kufa P

更新日期：2017-10-01 00:00:00

abstract：:Noninvasive body shaping is becoming a growing demand. The aim of this study was to investigate the efficacy and safety of the combined treatments of 1064 nm Nd:YAG and 2940 nm Er:YAG in noninvasive lipolysis and skin tightening. Ten females were enrolled, and all women's side of the waist or the lower part of the abd...

journal_title：Journal of biophotonics

authors： Vas K,Besenyi Z,Urbán S,Badawi A,Pávics L,Erős G,Kemény L

更新日期：2019-09-01 00:00:00

abstract：:Localization microscopy methods like Stochastic Optical Reconstruction Microscopy (STORM) are very well suited for exploring clustering of proteins, as the data inherently provide a list of molecular coordinates. Here we use state-of-art cluster analysis algorithms (DBSCAN) to explore the clustering behaviour of diffe...

journal_title：Journal of biophotonics

authors： Persson H,Potrzebowski W,Potrzebowska K,Svensson LM

更新日期：2019-03-01 00:00:00

abstract：:We have developed a reflection-mode switchable subwavelength Bessel-beam (BB) and Gaussian-beam (GB) photoacoustic microscopy (PAM) system. To achieve both reflection-mode and high resolution, we tightly attached a very small ultrasound transducer to an optical objective lens with numerical aperture of 1.0 and working...

journal_title：Journal of biophotonics

authors： Park B,Lee H,Jeon S,Ahn J,Kim HH,Kim C

更新日期：2019-02-01 00:00:00

abstract：:Stereotaxic instruments are increasingly used in research animals for the study of disease, but typically require restraints and anesthetic procedures. A stereotaxic head mount that enables imaging of the anterior chamber of the eye in alert and freely mobile mice is presented in this study. The head mount is fitted b...

journal_title：Journal of biophotonics

authors： Paulson B,Lee S,Jue M,Lee K,Lee S,Kim GB,Moon Y,Lee JY,Kim N,Kim JK

更新日期：2020-05-01 00:00:00

abstract：:Semen analysis is performed for evaluation of fertility disorders, however it is susceptible to subjectivity of investigators, and lacking of objective criterion for sperm cell quality remains a problem. There is an ongoing debate on which criteria should be employed to define normal spermatozoa. Here, the aim of our ...

journal_title：Journal of biophotonics

authors： Huang Z,Chen G,Chen X,Wang J,Chen J,Lu P,Chen R

更新日期：2014-09-01 00:00:00

abstract：:The possibilities of using Mueller matrix (MM) imaging polarimetry to assess meat quality have not yet been sufficiently explored. In the current study, the fresh porcine muscles are imaged at room temperature with a wide-field MM imaging polarimeter over 26 hours to visualize dynamics of tissue optical properties thr...

journal_title：Journal of biophotonics

authors： Peyvasteh M,Popov A,Bykov A,Pierangelo A,Novikova T,Meglinski I

更新日期：2020-11-21 00:00:00

abstract：:For the screening purposes urine is an especially attractive biofluid, since it offers easy and noninvasive sample collection and provides a snapshot of the whole metabolic status of the organism, which may change under different pathological conditions. Raman spectroscopy (RS) has the potential to monitor these chang...

journal_title：Journal of biophotonics

authors： Žukovskaja O,Ryabchykov O,Straßburger M,Heinekamp T,Brakhage AA,Hennings CJ,Hübner CA,Wegmann M,Cialla-May D,Bocklitz TW,Weber K,Popp J

更新日期：2020-01-01 00:00:00