Laser-induced plasmon-mediated treatment of retinoblastoma in viscous vitreous phantom.

Abstract:

:Retinoblastoma (RB) is a rare form of cancer of the retina most prevalent in young children. We successfully show that laser-induced cell disruption, mediated by gold plasmonic nanoparticle (NP), is a potential and efficient therapy to kill the cancerous cells. The proof of concept is demonstrated in vitro on cultured Y79 RB cancer cells with a nanosecond laser at 527 nm, for both attached cells at the bottom of a Petri dish and for floating, clustered cells in a viscous vitreous phantom comprised of hyaluronan. We report a cellular death of 82% after irradiation in classic culture medium and a cellular death of 98% in vitreous phantom, for similar number of NPs in each sample. It is found that the NPs efficiently penetrate the floating Y79 clusters cells in the vitreous phantom, leading to a cellular death of over 85% even within the centre of the aggregates. The proposed treatment technique is based on a similar nanosecond laser used to eliminate floaters in the vitreous, but with much lower (100-1000 times) fluences of 20 J cm-2 .

journal_name

J Biophotonics

journal_title

Journal of biophotonics

authors

Darviot C,Hardy P,Meunier M

doi

10.1002/jbio.201900193

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

e201900193

issue

11

eissn

1864-063X

issn

1864-0648

journal_volume

12

pub_type

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