Brillouin spectroscopy as a new method of screening for increased CSF total protein during bacterial meningitis.

Abstract:

:Bacterial meningitis is a disease of pronounced clinical significance, especially in the developing world. Immediate treatment with antibiotics is essential, and no single test can provide a conclusive diagnosis. It is well established that elevated total protein in cerebrospinal fluid (CSF) is associated with bacterial meningitis. Brillouin spectroscopy is a widely used optical technique for noninvasive determination of the elastic moduli of materials. We found that elevated protein levels in CSF alter the fluid elasticity sufficiently to be measurable by Brillouin spectroscopy, with model healthy and diseased fluids distinguishable to marked significance (P = 0.014), which increases with sample concentration by dialysis. Typical raw output of a 2-stage VIPA Brillouin spectrometer: inelastically scattered Brillouin peaks (arrows) and elastically scattered incident radiation (center cross).

journal_name

J Biophotonics

journal_title

Journal of biophotonics

authors

Steelman Z,Meng Z,Traverso AJ,Yakovlev VV

doi

10.1002/jbio.201400047

subject

Has Abstract

pub_date

2015-05-01 00:00:00

pages

408-14

issue

5

eissn

1864-063X

issn

1864-0648

journal_volume

8

pub_type

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