Abstract:
:In vivo microscopy has recently become a gold standard in lung immunology studies involving small animals, largely benefiting from the democratization of multiphoton microscopy allowing for deep tissue imaging. This technology represents currently our only way of exploring the lungs and inferring what happens in human respiratory medicine. The interest of lung in vivo microscopy essentially relies upon its relevance as a study model, fulfilling physiological requirements in comparison with in vitro and ex vivo experiments. However, strategies developed in order to overcome movements of the thorax caused by breathing and heartbeats remain the chief drawback of the technique and a major source of invasiveness. In this context, minimizing invasiveness is an unavoidable prerequisite for any improvement of lung in vivo microscopy. This review puts into perspective the main techniques enabling lung in vivo microscopy, providing pros and cons regarding invasiveness.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Fiole D,Tournier JNdoi
10.1002/jbio.201500246subject
Has Abstractpub_date
2016-09-01 00:00:00pages
868-78issue
9eissn
1864-063Xissn
1864-0648journal_volume
9pub_type
杂志文章,评审abstract::Several studies have demonstrated the potential of cross-polarization optical coherence tomography (CP-OCT) to quantify the severity of early caries lesions (tooth decay) on tooth surfaces. The purpose of this study is to show that 2D images of the lesion depth and the integrated reflectivity can be used to accurately...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201300137
更新日期:2015-01-01 00:00:00
abstract::There has been increasing use of in vitro cell culture models that more realistically replicate the three-dimensional (3D) environment found in vivo. Multicellular tumor spheroids (MTS) using cell lines or patient-derived organoids have become an important in vitro drug development tool, where cells are grown in a 3D ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800201
更新日期:2019-05-01 00:00:00
abstract::In this paper we introduce a custom scanning near-field optical microscope (SNOM) that simultaneously collects reflection and transmission near-field images along with topography. This dual-optical SNOM uses a bent probe, which allows for axial reflection imaging, accurate surface scanning, and easy identification of ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201000022
更新日期:2011-03-01 00:00:00
abstract::The use of conventional fluorescence microscopy for characterizing tissue pathological states is limited by overlapping spectra and the dependence on excitation power and fluorophore concentration. Fluorescence lifetime imaging microscopy (FLIM) can overcome these limitations due to its insensitivity to fluorophore co...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200202
更新日期:2014-07-01 00:00:00
abstract::Lasers are used in dentistry for a variety of indications. One of these is the disinfection of root canals or the sterilization of residual caries. Many studies have demonstrated the capacity to kill bacteria for lasers but the fundamental mechanism of the laser effect remains quite unclear. With our experiments we wa...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201000007
更新日期:2010-06-01 00:00:00
abstract::Alveolar type II (ATII) cells in the peripheral human lung spontaneously differentiate toward ATI cells, thus enabling air-blood barrier formation. Here, linear Raman and coherent anti-Stokes Raman scattering (CARS) microscopy are applied to study cell differentiation of freshly isolated ATII cells. The Raman spectra ...
journal_title:Journal of biophotonics
pub_type: 社论
doi:10.1002/jbio.201800052
更新日期:2019-06-01 00:00:00
abstract::Single-molecule localisation based super-resolution fluorescence imaging produces maps of the coordinates of fluorescent molecules in a region of interest. Cluster analysis algorithms provide information concerning the clustering characteristics of these molecules, often through the generation of cluster heat maps bas...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201400127
更新日期:2015-11-01 00:00:00
abstract::We present the design and construction of a prototype imaging probe capable of co-registered pulse-echo ultrasound and photoacoustic (optoacoustic) imaging in real time. The probe consists of 36 fibers of 200 micron core diameter each that are distributed around a commercial transvaginal ultrasound transducer, and hou...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200163
更新日期:2013-06-01 00:00:00
abstract::A wide variety of subcutaneous soft-tissue masses may be seen in clinical practice. Clinical examination based on palpation alone is often insufficient to identify the nature and exact origin of the mass, in which case imaging is necessary. We used handheld multispectral optoacoustic imaging technology (MSOT) in a pro...
journal_title:Journal of biophotonics
pub_type: 信件
doi:10.1002/jbio.201600274
更新日期:2017-08-01 00:00:00
abstract::Stimulated Raman scattering (SRS) microscopy is a label-free method generating images based on chemical contrast within samples, and has already shown its great potential for high-sensitivity and fast imaging of biological specimens. The capability of SRS to collect molecular vibrational signatures in bio-samples, cou...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900028
更新日期:2019-09-01 00:00:00
abstract::Lasers/LEDs demonstrate therapeutic effects for a range of biomedical applications. However, a consensus on effective light irradiation parameters and efficient and reliable measurement techniques remain limited. The objective here is to develop, characterise and demonstrate the application of LED arrays in order to p...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600273
更新日期:2017-11-01 00:00:00
abstract::Whole slide imaging (WSI) has moved digital pathology closer to diagnostic practice in recent years. Due to the inherent tissue topography variability, accurate autofocusing remains a critical challenge for WSI and automated microscopy systems. The traditional focus map surveying method is limited in its ability to ac...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.202000227
更新日期:2020-12-01 00:00:00
abstract::Optical coherence tomography (OCT) is a well-developed technology that utilizes near-infrared light to reconstruct three-dimensional images of biological tissues with micrometer resolution. Improvements of the imaging contrast of the OCT technique are able to further widen its extensive biomedical applications. In thi...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600047
更新日期:2017-06-01 00:00:00
abstract::We developed an optical imaging method based on a feedback principle in which the specific scan pattern is adapted according to the shape of the sample. The feedback approach produces nanometer-resolved 3D images of very small and moving features in live cells in seconds. We show images of microvilli in live cultured ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201100002
更新日期:2011-06-01 00:00:00
abstract::High-resolution deformation measurements in a functionally graded hard tissue such as human dentin are essential to understand the unbound water-loss mediated changes and their role in its mechanical integrity. Yet a whole-field, 3-dimensional (3D) measurement and characterization of fully hydrated dentin in both macr...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700368
更新日期:2018-09-01 00:00:00
abstract::Dynamic follow-up of exogenous molecules permeation through the skin is one among many competing applications for confocal Raman microspectroscopy. Previous studies showed the feasibility of tracking actives through the skin; the next step should be recording in vivo kinetics. Thus, we conducted a study to evaluate th...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201300011
更新日期:2014-09-01 00:00:00
abstract::Achieving a maximal safe extent of resection during brain tumor surgery is the goal for improved patient prognosis. Fluorescence-guided neurosurgery using 5-aminolevulinic acid (5-ALA) induced protoporphyrin IX has thereby become a valuable tool enabling a high frequency of complete resections and a prolonged progress...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800378
更新日期:2019-06-01 00:00:00
abstract::The aim of the present study is to optimize parameters for inhibiting neuronal activity safely and investigating thermal inhibition of rat cortex neural networks in vitro by continuous infrared (IR) laser. Rat cortex neurons were cultured on multi-electrode arrays until neural networks were formed with spontaneous neu...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800403
更新日期:2019-07-01 00:00:00
abstract::Noninvasive body shaping is becoming a growing demand. The aim of this study was to investigate the efficacy and safety of the combined treatments of 1064 nm Nd:YAG and 2940 nm Er:YAG in noninvasive lipolysis and skin tightening. Ten females were enrolled, and all women's side of the waist or the lower part of the abd...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900083
更新日期:2019-09-01 00:00:00
abstract::We report on transient membrane perforation of living cancer cells using plasmonic gold nanoparticles (AuNPs) enhanced single near infrared (NIR) femtosecond (fs) laser pulse. Under optimized laser energy fluence, single pulse treatment (τ = 45 fs, λ = 800 nm) resulted in 77% cell perforation efficiency and 90% cell v...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201500135
更新日期:2016-01-01 00:00:00
abstract::One pertinent complication in bacterial infection is the growth of biofilms, that is, communities of surface-adhered bacteria resilient to antibiotics. Photodynamic inactivation (PDI) has been proposed as an alternative to antibiotic treatment; however, novel techniques complementing standard efficacy measures are req...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800018
更新日期:2018-10-01 00:00:00
abstract::DsRed is representative of the tetrameric reef coral fluorescent proteins that constitute particularly interesting coupled multichromophoric systems. Either a green emitting or a red emitting chromophore can form within each of the monomers of the protein tetramer. Within the tetramers the chromophores are thought to ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201000075
更新日期:2011-01-01 00:00:00
abstract::Basidiomycetes, that is, mushroom-type fungi, are known to produce pigments in response to environmental impacts. As antioxidants with a high level of unsaturation, these compounds can neutralize highly oxidative species. In the event of close contact with other microbes, the enzymatically controlled pigment productio...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700369
更新日期:2018-06-01 00:00:00
abstract::The capabilities of cross-polarization optical coherence tomography (CP OCT) for early bladder-cancer detection are assessed in statistical study and compared with the traditional OCT. Unlike the traditional OCT that demonstrates images only in copolarization, CP OCT acquires images in cross-polarization and copolariz...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201000088
更新日期:2011-08-01 00:00:00
abstract::The research work presented in this paper focuses on qualitative tissue differentiation by monitoring the intensity ratios of atomic emissions using 'Laser Induced Breakdown Spectroscopy' (LIBS) on the plasma plume created during laser tissue ablation. The background of this study is to establish a real time feedback ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201300159
更新日期:2015-01-01 00:00:00
abstract::In photoacoustic tomography (PAT), a tunable laser typically illuminates the tissue at multiple wavelengths, and the received photoacoustic waves are used to form functional images of relative total haemoglobin (rHbT) and blood oxygenation saturation (%sO2 ). Due to measurement errors, the estimation of these paramete...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000368
更新日期:2020-12-30 00:00:00
abstract::An integrated multimodal optical microscope is demonstrated for high-resolution, structural and functional imaging of engineered and natural skin. This microscope incorporates multiple imaging modalities including optical coherence (OCM), multi-photon (MPM), and fluorescence lifetime imaging microscopy (FLIM), enablin...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200003
更新日期:2012-05-01 00:00:00
abstract::Low-level laser therapy (LLLT) is known to enhance mitochondrial electron transfer and ATP production; thus, this study asked whether LLLT could stimulate the oxidative burst in human neutrophils (PMN) and improve their ability to kill microorganisms. Blood from healthy human subjects was collected and PMN were isolat...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600035
更新日期:2016-12-01 00:00:00
abstract::As the field of tissue engineering evolves, there will be an increasingly important need to visualize and track the complex dynamic changes that occur within three-dimensional constructs. Optical coherence tomography (OCT), as an emerging imaging technology applied to biological materials, offers a number of significa...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.200910048
更新日期:2009-11-01 00:00:00
abstract::Cerebral malaria (CM) is a severe complication of Plasmodium falciparum infection associated with impaired cerebral blood flow. Visualization of the eye vasculature, which is embryologically derived from that of the brain, is used clinically to diagnose the syndrome. Here, we introduce camera-phone laser speckle imagi...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800098
更新日期:2019-01-01 00:00:00