Stereotaxic endoscopy for the ocular imaging of awake, freely moving animal models.

Abstract:

:Stereotaxic instruments are increasingly used in research animals for the study of disease, but typically require restraints and anesthetic procedures. A stereotaxic head mount that enables imaging of the anterior chamber of the eye in alert and freely mobile mice is presented in this study. The head mount is fitted based on computed tomography scans and manufactured using 3D printing. The system is placed noninvasively using temporal mount bars and a snout mount, without breaking the skin or risking suffocation, while an instrument channel stabilizes the ocular probes. With a flexible micro-endoscopic probe and a confocal scanning laser microscopy system, <20 μm resolution is achieved in vivo with a field of view of nearly 1 mm. Discomfort is minimal, and further adaptations for minimally invasive neuroscience, optogenetics and auditory studies are possible.

journal_name

J Biophotonics

journal_title

Journal of biophotonics

authors

Paulson B,Lee S,Jue M,Lee K,Lee S,Kim GB,Moon Y,Lee JY,Kim N,Kim JK

doi

10.1002/jbio.201960188

subject

Has Abstract

pub_date

2020-05-01 00:00:00

pages

e201960188

issue

5

eissn

1864-063X

issn

1864-0648

journal_volume

13

pub_type

杂志文章
  • Enhanced photoinactivation of Staphylococcus aureus with nanocomposites containing plasmonic particles and hematoporphyrin.

    abstract::We fabricated composite nanoparticles consisting of a plasmonic core (gold nanorods or gold-silver nanocages) and a hematoporphyrin-doped silica shell. The dual photodynamic and photothermal activities of such nanoparticles against Staphylococcus aureus 209 P were studied and compared with the activities of reference ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201200079

    authors: Khlebtsov BN,Tuchina ES,Khanadeev VA,Panfilova EV,Petrov PO,Tuchin VV,Khlebtsov NG

    更新日期:2013-04-01 00:00:00

  • Quantitative study of optical and mechanical bone status using multispectral photoacoustics.

    abstract::Osteoporosis is a major public health problem worldwide. Here, we present a quantitative multispectral photoacoustic method for the evaluation of bone pathologies which has significant advantages over pure ultrasonic or pure optical methods as it provides both molecular information and bone mechanical status. This is ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201500206

    authors: Steinberg I,Turko N,Levi O,Gannot I,Eyal A

    更新日期:2016-09-01 00:00:00

  • Integrated multimodal optical microscopy for structural and functional imaging of engineered and natural skin.

    abstract::An integrated multimodal optical microscope is demonstrated for high-resolution, structural and functional imaging of engineered and natural skin. This microscope incorporates multiple imaging modalities including optical coherence (OCM), multi-photon (MPM), and fluorescence lifetime imaging microscopy (FLIM), enablin...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201200003

    authors: Zhao Y,Graf BW,Chaney EJ,Mahmassani Z,Antoniadou E,Devolder R,Kong H,Boppart MD,Boppart SA

    更新日期:2012-05-01 00:00:00

  • Imaging blood flow inside highly scattering media using ultrasound modulated optical tomography.

    abstract::We report the use of ultrasound modulated optical tomography (UOT) with heterodyne parallel detection to locally sense and image blood flow deep inside a highly scattering medium. We demonstrate that the UOT signal is sensitive to the speed of the blood flow in the ultrasound focus and present an analytical model that...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201700013

    authors: Hussain A,Steenbergen W,Vellekoop IM

    更新日期:2018-01-01 00:00:00

  • High fluence light emitting diode-generated red light modulates characteristics associated with skin fibrosis.

    abstract::Skin fibrosis, often referred to as skin scarring, is a significant international health problem with limited treatment options. The hallmarks of skin fibrosis are increased fibroblast proliferation, collagen production, and migration speed. Recently published clinical observations indicate that visible red light may ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201600059

    authors: Mamalis A,Koo E,Garcha M,Murphy WJ,Isseroff RR,Jagdeo J

    更新日期:2016-12-01 00:00:00

  • Evolution of raw meat polarization-based properties by means of Mueller matrix imaging.

    abstract::The possibilities of using Mueller matrix (MM) imaging polarimetry to assess meat quality have not yet been sufficiently explored. In the current study, the fresh porcine muscles are imaged at room temperature with a wide-field MM imaging polarimeter over 26 hours to visualize dynamics of tissue optical properties thr...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.202000376

    authors: Peyvasteh M,Popov A,Bykov A,Pierangelo A,Novikova T,Meglinski I

    更新日期:2020-11-21 00:00:00

  • Inhibition of cortical neural networks using infrared laser.

    abstract::The aim of the present study is to optimize parameters for inhibiting neuronal activity safely and investigating thermal inhibition of rat cortex neural networks in vitro by continuous infrared (IR) laser. Rat cortex neurons were cultured on multi-electrode arrays until neural networks were formed with spontaneous neu...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201800403

    authors: Xia Q,Nyberg T

    更新日期:2019-07-01 00:00:00

  • Determination of the metabolic index using the fluorescence lifetime of free and bound nicotinamide adenine dinucleotide using the phasor approach.

    abstract::The fluorescence lifetime of nicotinamide adenine dinucleotide (NADH) is commonly used in conjunction with the phasor approach as a molecular biomarker to provide information on cellular metabolism of autofluorescence imaging of cells and tissue. However, in the phasor approach, the bound and free lifetime defining th...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201900156

    authors: Ranjit S,Malacrida L,Stakic M,Gratton E

    更新日期:2019-11-01 00:00:00

  • Assesment of apoptosis induced changes in scattering using optical coherence tomography.

    abstract::The aim of this study is to identify changes in scattering with optical coherence tomography (OCT) and relate these measurements with mitochondrial changes during the initiation of apoptosis. Human retinal pigment epithelial cells were cultured and apoptosis was induced using 10% alcohol. Using the attenuation coeffic...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201500198

    authors: de Bruin DM,Broekgaarden M,van Gemert MJ,Heger M,de la Rosette JJ,Van Leeuwen TG,Faber DJ

    更新日期:2016-09-01 00:00:00

  • Effect of tissue staining in quantitative phase imaging.

    abstract::Quantitative phase imaging (QPI) is an emerging modality, which enables the identification of abnormalities in tissue based on optical properties. QPI can be applied to any biological specimen due to its label-free imaging capability, but its use in stained tissue is unclear. Here, we study the variability of QPI with...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201700402

    authors: Ban S,Min E,Ahn Y,Popescu G,Jung W

    更新日期:2018-08-01 00:00:00

  • Nanometer-scale imaging by the modulation tracking method.

    abstract::We developed an optical imaging method based on a feedback principle in which the specific scan pattern is adapted according to the shape of the sample. The feedback approach produces nanometer-resolved 3D images of very small and moving features in live cells in seconds. We show images of microvilli in live cultured ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201100002

    authors: Lanzano L,Digman MA,Fwu P,Giral H,Levi M,Gratton E

    更新日期:2011-06-01 00:00:00

  • Therapeutic prognosis of prostate cancer using surface-enhanced Raman scattering of patient urine and multivariate statistical analysis.

    abstract::Surface-enhanced Raman scattering (SERS) is highly sensitive and label-free analytical technique based on Raman spectroscopy aided by field-multiplying plasmonic nanostructures. We report the use of SERS measurements of patient urine in conjunction with biostatistical algorithms to assess the treatment response of pro...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.202000275

    authors: Ma Y,Chi J,Zheng Z,Attygalle A,Kim IY,Du H

    更新日期:2021-01-01 00:00:00

  • In vivo sun protection factor and UVA protection factor determination using (hybrid) diffuse reflectance spectroscopy and a multi-lambda-LED light source.

    abstract::The sun protection factor (SPF) values are currently determined using an invasive procedure, in which the volunteers are irradiated with ultraviolet (UV) light. Non-invasive approaches based on hybrid diffuse reflectance spectroscopy (HDRS) have shown a good correlation with conventional SPF testing. Here, we present ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.202000348

    authors: Throm CM,Wiora G,Reble C,Schleusener J,Schanzer S,Karrer H,Kolbe L,Khazaka G,Meinke MC,Lademann J

    更新日期:2020-10-06 00:00:00

  • The effects of low level laser therapy on both HIV-1 infected and uninfected TZM-bl cells.

    abstract::Human immunodeficiency virus (HIV-1) infection remains a major health problem despite the use of highly active antiretroviral therapy (HAART), which has greatly reduced mortality rates. Due to the unavailability of an effective vaccine and treatment that would completely eradicate the virus in infected individuals, th...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201600217

    authors: Lugongolo MY,Manoto SL,Ombinda-Lemboumba S,Maaza M,Mthunzi-Kufa P

    更新日期:2017-10-01 00:00:00

  • In vivo noninvasive measurement of spatially resolved corneal elasticity in human eyes using Lamb wave optical coherence elastography.

    abstract::Current elastography techniques are limited in application to accurately assess spatially resolved corneal elasticity in vivo for human eyes. The air-puff optical coherence elastography (OCE) with an eye motion artifacts correction algorithm is developed to distinguish the in vivo cornea vibration from the eye motion ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.202000104

    authors: Jin Z,Chen S,Dai Y,Bao C,Ye S,Zhou Y,Wang Y,Huang S,Wang Y,Shen M,Zhu D,Lu F

    更新日期:2020-08-01 00:00:00

  • The microbial threat: Can rare earths help?

    abstract::Despite an ever increasing demand for reliable and cheap methods in the detection and quantification of microbes, surprisingly few investigations have explored or utilized the luminescence of rare earths in the microbial context, neither in conventional, that is, plating and microscopic imaging techniques, nor in adva...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.202000068

    authors: Lezhnina MM,Rochowiak W,Göhde W,Kuczius R,Kynast U

    更新日期:2020-09-01 00:00:00

  • Optical clearing for multiscale biological tissues.

    abstract::Three-dimensional reconstruction of tissue structures is essential for biomedical research. The development of light microscopes and various fluorescent labeling techniques provides powerful tools for this motivation. However, optical imaging depth suffers from strong light scattering due to inherent heterogeneity of ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审

    doi:10.1002/jbio.201700187

    authors: Yu T,Qi Y,Gong H,Luo Q,Zhu D

    更新日期:2018-02-01 00:00:00

  • Towards Raman spectroscopy of urine as screening tool.

    abstract::For the screening purposes urine is an especially attractive biofluid, since it offers easy and noninvasive sample collection and provides a snapshot of the whole metabolic status of the organism, which may change under different pathological conditions. Raman spectroscopy (RS) has the potential to monitor these chang...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201900143

    authors: Žukovskaja O,Ryabchykov O,Straßburger M,Heinekamp T,Brakhage AA,Hennings CJ,Hübner CA,Wegmann M,Cialla-May D,Bocklitz TW,Weber K,Popp J

    更新日期:2020-01-01 00:00:00

  • Mechanism of high-power NIR laser bacteria inactivation.

    abstract::Lasers are used in dentistry for a variety of indications. One of these is the disinfection of root canals or the sterilization of residual caries. Many studies have demonstrated the capacity to kill bacteria for lasers but the fundamental mechanism of the laser effect remains quite unclear. With our experiments we wa...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201000007

    authors: Hibst R,Graser R,Udart M,Stock K

    更新日期:2010-06-01 00:00:00

  • Laser imaging polarimetry of nacre.

    abstract::Nacre is a complex biomaterial made of aragonite-tablet bricks and organic mortar that is considerably resilient against breakage. Nacre has been studied with a wide range of laboratory techniques, leading to understanding key fundamentals and informing the creation of bio-inspired materials. In this article, we prese...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201800026

    authors: Jones JA,Metzler RA,D'Addario AJ,Burgess C,Regan B,Spano S,Cvarch BA,Galvez EJ

    更新日期:2018-10-01 00:00:00

  • Exploring photoinactivation of microbial biofilms using laser scanning microscopy and confined 2-photon excitation.

    abstract::One pertinent complication in bacterial infection is the growth of biofilms, that is, communities of surface-adhered bacteria resilient to antibiotics. Photodynamic inactivation (PDI) has been proposed as an alternative to antibiotic treatment; however, novel techniques complementing standard efficacy measures are req...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201800018

    authors: Thomsen H,Graf FE,Farewell A,Ericson MB

    更新日期:2018-10-01 00:00:00

  • In vivo pump-probe optical coherence tomography imaging in Xenopus laevis.

    abstract::Currently, optical coherence tomography (OCT), is not capable of obtaining molecular information often crucial for identification of disease. To enable molecular imaging with OCT, we have further developed a technique that harnesses transient changes in light absorption in the sample to garner molecular information. A...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201300119

    authors: Carrasco-Zevallos O,Shelton RL,Kim W,Pearson J,Applegate BE

    更新日期:2015-01-01 00:00:00

  • Scattering based hyperspectral imaging of plasmonic nanoplate clusters towards biomedical applications.

    abstract::A new optical scattering contrast-agent based on polymer-nanoparticle encapsulated silver nanoplates (PESNs) is presented. Silver nanoplates were chosen due to the flexibility of tuning their plasmon frequencies. The polymer coating preserves their physical and optical properties and confers other advantages such as c...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201500177

    authors: Ray A,Kopelman R,Chon B,Briggman K,Hwang J

    更新日期:2016-07-01 00:00:00

  • Prognostic analysis of amyotrophic lateral sclerosis based on clinical features and plasma surface-enhanced Raman spectroscopy.

    abstract::Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease with a wide range of survival times. We aimed to explore prognostic factors related to short survival based on clinical features and plasma metabolic signatures using surface-enhanced Raman spectroscopy (SERS). One hundred and thirty-eight sporad...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201900012

    authors: Zhang QJ,Chen Y,Zou XH,Hu W,Lin XL,Feng SY,Chen F,Xu LQ,Chen WJ,Wang N

    更新日期:2019-08-01 00:00:00

  • A novel urine analysis technique combining affinity chromatography with Au nanoparticle based surface enhanced Raman spectroscopy for potential applications in non-invasive cancer screening.

    abstract::Modified nucleoside in urine samples is one of the most common biomarkers for cancer screening. Therefore, we developed a novel detection method for modified nucleoside detection in human urine. In this work, the modified nucleoside from real cancer patient's urine samples was first separated and purified using the af...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201800327

    authors: Lin X,Wang L,Lin H,Lin D,Lin J,Liu X,Qiu S,Xu Y,Chen G,Feng S

    更新日期:2019-04-01 00:00:00

  • In vivo noninvasive visualization of retinal perfusion dysfunction in murine cerebral malaria by camera-phone laser speckle imaging.

    abstract::Cerebral malaria (CM) is a severe complication of Plasmodium falciparum infection associated with impaired cerebral blood flow. Visualization of the eye vasculature, which is embryologically derived from that of the brain, is used clinically to diagnose the syndrome. Here, we introduce camera-phone laser speckle imagi...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201800098

    authors: Remer I,Pierre-Destine LF,Tay D,Golightly LM,Bilenca A

    更新日期:2019-01-01 00:00:00

  • Dual-wavelength-assisted thermal hemostasis for treatment of benign prostate hyperplasia.

    abstract::Laser treatment on a large size of prostate gland often encounters significant bleeding that can prolong the entire procedure and cause urinary complications. The current study investigates the feasibility of dual-wavelength (532 and 980 nm) application to achieve rapid hemostasis for 532-nm laser prostatectomy. Porci...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201700192

    authors: Hwang J,Kim H,Truong GV,Xuan J,Hasenberg T,Kang HW

    更新日期:2018-04-01 00:00:00

  • A simple approach for non-invasive transcranial optical vascular imaging (nTOVI).

    abstract::In vivo imaging of cerebral vasculature is highly vital for clinicians and medical researchers alike. For a number of years non-invasive optical-based imaging of brain vascular network by using standard fluorescence probes has been considered as impossible. In the current paper controverting this paradigm, we present ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201400140

    authors: Kalchenko V,Israeli D,Kuznetsov Y,Meglinski I,Harmelin A

    更新日期:2015-11-01 00:00:00

  • Nanoscale distinction of membrane patches--a TERS study of Halobacterium salinarum.

    abstract::The structural organization of cellular membranes has an essential influence on their functionality. The membrane surfaces currently are considered to consist of various distinct patches, which play an important role in many processes, however, not all parameters such as size and distribution are fully determined. In ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201100131

    authors: Deckert-Gaudig T,Böhme R,Freier E,Sebesta A,Merkendorf T,Popp J,Gerwert K,Deckert V

    更新日期:2012-07-01 00:00:00

  • Four-dimensional imaging of murine subpleural alveoli using high-speed optical coherence tomography.

    abstract::The investigation of lung dynamics on alveolar scale is crucial for the understanding and treatment of lung diseases, such as acute lung injury and ventilator induced lung injury, and to promote the development of protective ventilation strategies. One approach to this is the establishment of numerical simulations of ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201200027

    authors: Kirsten L,Gaertner M,Schnabel C,Meissner S,Koch E

    更新日期:2013-02-01 00:00:00