Optical investigations of the RNA polymerase molecular motor.

Abstract:

:Molecular motors have been extensively studied over the past 25 years using a range of optical microscopy methods. The specific example of RNA polymerase, responsible for transcribing the information encoded in DNA into RNA, illustrates the strength of the convergence between these recent approaches and the vast store of data collected over the past half century using the more "classical" approaches of genetics, biochemistry, and structure elucidation.

journal_name

J Biophotonics

journal_title

Journal of biophotonics

authors

Strick TR

doi

10.1002/jbio.200810030

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

269-79

issue

4

eissn

1864-063X

issn

1864-0648

journal_volume

1

pub_type

杂志文章
  • Measurement of cartilage sub-component distributions through the surface by Raman spectroscopy-based multivariate analysis.

    abstract::Articular cartilage posesses unique material properties due to a complex depth-dependent composition of sub-components. Raman spectroscopy has proven valuable in quantifying this composition through cartilage cross-sections. However, cross-sectioning requires tissue destruction and is not practical in situ. In this wo...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.202000289

    authors: Mason D,Murugkar S,Speirs AD

    更新日期:2021-01-01 00:00:00

  • Monitoring caffeine and resveratrol cutaneous permeation by confocal Raman microspectroscopy.

    abstract::Dynamic follow-up of exogenous molecules permeation through the skin is one among many competing applications for confocal Raman microspectroscopy. Previous studies showed the feasibility of tracking actives through the skin; the next step should be recording in vivo kinetics. Thus, we conducted a study to evaluate th...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201300011

    authors: Tfaili S,Josse G,Angiboust JF,Manfait M,Piot O

    更新日期:2014-09-01 00:00:00

  • Corneal tomographic features of postrefractive surgery ectasia.

    abstract::The purpose of this study was to evaluate the tomographic features of postrefractive surgery eyes. This was a retrospective evaluation of clinical data. Three patients with post-LASIK (laser-assisted in situ keratomileusis) and two patients with post-SMILE (small incision lenticule extraction) ectasia were imaged with...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201800253

    authors: Khamar P,Dalal R,Chandapura R,Francis M,Shetty R,Nelson EJR,Nuijts RMMA,Sinha Roy A

    更新日期:2019-02-01 00:00:00

  • Unique spectral signature of human cutaneous squamous cell carcinoma by photoacoustic imaging.

    abstract::Cutaneous squamous cell carcinoma (cSCC) is a common skin cancer with metastatic potential. To reduce reoperations due to nonradical excision, there is a need to develop a technique for identification of tumor margins preoperatively. Photoacoustic (PA) imaging is a novel imaging technology that combines the strengths ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201960212

    authors: Hult J,Dahlstrand U,Merdasa A,Wickerström K,Chakari R,Persson B,Cinthio M,Erlöv T,Albinsson J,Gesslein B,Sheikh R,Malmsjö M

    更新日期:2020-05-01 00:00:00

  • Exploring the effects of low-level laser therapy on fibroblasts and tumor cells following gamma radiation exposure.

    abstract::Ionizing radiation (IR) induces DNA damage and low-level laser therapy (LLLT) has been investigated to prevent or repair detrimental outcomes resulting from IR exposure. Few in vitro studies, however, explore the biological mechanisms underlying those LLLT benefits. Thus, in this work, fibroblasts and tumor cells are ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201600107

    authors: Ramos Silva C,Cabral FV,de Camargo CF,Núñez SC,Mateus Yoshimura T,de Lima Luna AC,Maria DA,Ribeiro MS

    更新日期:2016-12-01 00:00:00

  • Chemically-specific dual/differential CARS micro-spectroscopy of saturated and unsaturated lipid droplets.

    abstract::We have investigated the ability of dual-frequency Coherent Antistokes Raman Scattering (D-CARS) micro-spectroscopy, based on femtosecond pulses (100 fs or 5 fs) spectrally focussed by glass dispersion, to distinguish the chemical composition of micron-sized lipid droplets consisting of different triglycerides types (...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201200197

    authors: Di Napoli C,Masia F,Pope I,Otto C,Langbein W,Borri P

    更新日期:2014-01-01 00:00:00

  • Low-power laser irradiation in salivary glands reduces glycemia in streptozotocin-induced diabetic female rats.

    abstract::Low-power laser irradiation (LPLI) has been extensively employed to modulate inflammation in vitro and in vivo. Previous reports from our group indicated that LPLI might regulate glycemia in diabetic animals. Diabetes results in chronic hyperglycemia and therefore chronic inflammation by upregulation of inflammatory m...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201600175

    authors: Fukuoka CY,Torres Schröter G,Nicolau J,Simões A

    更新日期:2016-12-01 00:00:00

  • Towards Raman spectroscopy of urine as screening tool.

    abstract::For the screening purposes urine is an especially attractive biofluid, since it offers easy and noninvasive sample collection and provides a snapshot of the whole metabolic status of the organism, which may change under different pathological conditions. Raman spectroscopy (RS) has the potential to monitor these chang...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201900143

    authors: Žukovskaja O,Ryabchykov O,Straßburger M,Heinekamp T,Brakhage AA,Hennings CJ,Hübner CA,Wegmann M,Cialla-May D,Bocklitz TW,Weber K,Popp J

    更新日期:2020-01-01 00:00:00

  • Chemically specific imaging and in-situ chemical analysis of articular cartilage with stimulated Raman scattering.

    abstract::Stimulated Raman scattering (SRS) has been applied to unstained samples of articular cartilage enabling the investigation of living cells within fresh tissue. Hyperspectral SRS measurements over the CH vibrational region showed variations in protein and lipid content within the cells, pericellular matrix and interterr...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201200213

    authors: Mansfield J,Moger J,Green E,Moger C,Winlove CP

    更新日期:2013-10-01 00:00:00

  • Eliminating the scattering ambiguity in multifocal, multimodal, multiphoton imaging systems.

    abstract::In this work we present how to entirely remove the scattering ambiguity present in existing multiphoton multifocal systems. This is achieved through the development and implementation of single-element detection systems that incorporate high-speed photon-counting electronics. These systems can be used to image entire ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201100139

    authors: Hoover EE,Field JJ,Winters DG,Young MD,Chandler EV,Speirs JC,Lapenna JT,Kim SM,Ding SY,Bartels RA,Wang JW,Squier JA

    更新日期:2012-05-01 00:00:00

  • Stain-free interferometric phase microscopy correlation with DNA fragmentation stain in human spermatozoa.

    abstract::Acridine orange (AO) staining is used to diagnose DNA fragmentation status in sperm cells. Interferometric phase microscopy (IPM) is an optical imaging method based on digital holographic microscopy that provides quantitative morphological and refractive index imaging of cells in vitro without the need for staining. W...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201800137

    authors: Barnea I,Karako L,Mirsky SK,Levi M,Balberg M,Shaked NT

    更新日期:2018-11-01 00:00:00

  • Sensitive multiplex detection of serological liver cancer biomarkers using SERS-active photonic crystal fiber probe.

    abstract::Surface-enhanced Raman scattering (SERS) spectroscopy possesses the most promising advantage of multiplex detection for biosensing applications, which is achieved due to the narrow 'fingerprint' Raman spectra from the analyte molecules. We developed an ultrasensitive platform for the multiplex detection of cancer biom...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201300084

    authors: Dinish US,Balasundaram G,Chang YT,Olivo M

    更新日期:2014-11-01 00:00:00

  • Fluorescence lifetime metabolic mapping of hypoxia-induced damage in pancreatic pseudo-islets.

    abstract::Pancreatic islet isolation from donor pancreases is an essential step for the transplantation of insulin-secreting β-cells as a therapy to treat type 1 diabetes mellitus. This process however damages islet basement membranes, which can lead to islet dysfunction or death. Posttransplantation, islets are further stresse...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.202000375

    authors: Zbinden A,Carvajal Berrio DA,Urbanczyk M,Layland SL,Bosch M,Fliri S,Lu CE,Jeyagaran A,Loskill P,Duffy GP,Schenke-Layland K

    更新日期:2020-12-01 00:00:00

  • Photoacoustic endoscopy: A progress review.

    abstract::Endoscopy has been widely used in biomedical imaging and integrated with various optical and acoustic imaging modalities. Photoacoustic imaging (PAI), one of the fastest growing biomedical imaging modalities, is a noninvasive and nonionizing method that owns rich optical contrast, deep acoustic penetration depth, mult...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审

    doi:10.1002/jbio.202000217

    authors: Guo H,Li Y,Qi W,Xi L

    更新日期:2020-12-01 00:00:00

  • A representation learning approach for recovering scatter-corrected spectra from Fourier-transform infrared spectra of tissue samples.

    abstract::Infrared spectra obtained from cell or tissue specimen have commonly been observed to involve a significant degree of scattering effects, often Mie scattering, which probably overshadows biochemically relevant spectral information by a nonlinear, nonadditive spectral component in Fourier transform infrared (FTIR) spec...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.202000385

    authors: Raulf AP,Butke J,Menzen L,Küpper C,Großerueschkamp F,Gerwert K,Mosig A

    更新日期:2020-12-08 00:00:00

  • High-resolution tomographic diffractive microscopy of biological samples.

    abstract::The authors have developed a tomographic diffractive microscope that combines microholography with illumination from an angular synthetic aperture. It images specimens relative to their complex index of refraction distribution (index and absorption) and permits imaging of unlabelled specimens, with high lateral resolu...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.200900094

    authors: Simon B,Debailleul M,Beghin A,Tourneur Y,Haeberlé O

    更新日期:2010-07-01 00:00:00

  • Microfluidics and photonics for Bio-System-on-a-Chip: a review of advancements in technology towards a microfluidic flow cytometry chip.

    abstract::Microfluidics and photonics come together to form a field commonly referred to as 'optofluidics'. Flow cytometry provides the field with a technology base from which both microfluidic and photonic components be developed and integrated into a useful device. This article reviews some of the more recent developments to ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审

    doi:10.1002/jbio.200810018

    authors: Godin J,Chen CH,Cho SH,Qiao W,Tsai F,Lo YH

    更新日期:2008-10-01 00:00:00

  • A robust ex vivo method to evaluate the diffusion properties of agents in biological tissues.

    abstract::A robust method is presented for evaluating the diffusion properties of chemicals in ex vivo biological tissues. Using this method that relies only on thickness and collimated transmittance measurements, the diffusion properties of glycerol, fructose, polypropylene glycol and water in muscle tissues were evaluated. Am...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201800333

    authors: Carneiro I,Carvalho S,Henrique R,Oliveira LM,Tuchin VV

    更新日期:2019-04-01 00:00:00

  • Safety and delivery efficiency of a photodynamic treatment of the lungs using indocyanine green and extracorporeal near infrared illumination.

    abstract::Photodynamic inactivation (PDI) is a promising alternative for combating infections caused by antimicrobial resistant bacteria. Pneumonias are among the most worrisome infections because of their high-mortality rate. Previous studies have demonstrated the feasibility of using PDI with extracorporeal light to treat pne...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.202000176

    authors: Kassab G,Cheburkanov V,Willis J,Moule MG,Kurachi C,Yakovlev V,Cirillo JD,Bagnato VS

    更新日期:2020-10-01 00:00:00

  • Enhanced photoinactivation of Staphylococcus aureus with nanocomposites containing plasmonic particles and hematoporphyrin.

    abstract::We fabricated composite nanoparticles consisting of a plasmonic core (gold nanorods or gold-silver nanocages) and a hematoporphyrin-doped silica shell. The dual photodynamic and photothermal activities of such nanoparticles against Staphylococcus aureus 209 P were studied and compared with the activities of reference ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201200079

    authors: Khlebtsov BN,Tuchina ES,Khanadeev VA,Panfilova EV,Petrov PO,Tuchin VV,Khlebtsov NG

    更新日期:2013-04-01 00:00:00

  • Near-infrared human finger measurements based on self-calibration point: Simulation and in vivo experiments.

    abstract::Near-infrared light allows measuring tissue oxygenation. These measurements relay on oxygenation-dependent absorption spectral changes. However, the tissue scattering, which is also spectral dependent, introduces an intrinsic error. Most methods focus on the volume reflectance from a semi-infinite sample. We have prop...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201700208

    authors: Duadi H,Feder I,Fixler D

    更新日期:2018-04-01 00:00:00

  • Optical assessment of cutaneous blood volume depends on the vessel size distribution: a computer simulation study.

    abstract::A Monte Carlo simulation was adapted to specify a skin model with pigmented epidermis, dermis with low baseline blood content, and vessels of a vascular lesion with an average added blood volume fraction of 5%. In the study, the lesion vessel diameters were increased and the number of lesion vessels decreased, such th...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.200900085

    authors: Jacques SL

    更新日期:2010-01-01 00:00:00

  • In vivo pump-probe optical coherence tomography imaging in Xenopus laevis.

    abstract::Currently, optical coherence tomography (OCT), is not capable of obtaining molecular information often crucial for identification of disease. To enable molecular imaging with OCT, we have further developed a technique that harnesses transient changes in light absorption in the sample to garner molecular information. A...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201300119

    authors: Carrasco-Zevallos O,Shelton RL,Kim W,Pearson J,Applegate BE

    更新日期:2015-01-01 00:00:00

  • Cross-polarization optical coherence tomography for early bladder-cancer detection: statistical study.

    abstract::The capabilities of cross-polarization optical coherence tomography (CP OCT) for early bladder-cancer detection are assessed in statistical study and compared with the traditional OCT. Unlike the traditional OCT that demonstrates images only in copolarization, CP OCT acquires images in cross-polarization and copolariz...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201000088

    authors: Gladkova N,Streltsova O,Zagaynova E,Kiseleva E,Gelikonov V,Gelikonov G,Karabut M,Yunusova K,Evdokimova O

    更新日期:2011-08-01 00:00:00

  • Fluorescence hyperspectral imaging (fHSI) using a spectrally resolved detector array.

    abstract::The ability to resolve multiple fluorescent emissions from different biological targets in video rate applications, such as endoscopy and intraoperative imaging, has traditionally been limited by the use of filter-based imaging systems. Hyperspectral imaging (HSI) facilitates the detection of both spatial and spectral...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201600304

    authors: Luthman AS,Dumitru S,Quiros-Gonzalez I,Joseph J,Bohndiek SE

    更新日期:2017-06-01 00:00:00

  • Hyperspectral multimodal CARS microscopy in the fingerprint region.

    abstract::A simple scheme for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy is based on the spectral focusing of ultrafast-oscillator-derived pump/probe light and synchronous photonic crystal fiber (PCF) fiber-generated broadband Stokes light. To date, such schemes allowed rapid hyperspectral imaging throug...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201200171

    authors: Pegoraro AF,Slepkov AD,Ridsdale A,Moffatt DJ,Stolow A

    更新日期:2014-01-01 00:00:00

  • Chromophore based analyses of steady-state diffuse reflectance spectroscopy: current status and perspectives for clinical adoption.

    abstract::Diffuse reflectance spectroscopy is a rapidly growing technology in the biophotonics community where it has shown promise in its ability to classify different tissues. In the steady-state domain a wide spectrum of clinical applications is supported with this technology ranging from diagnostic to guided interventions. ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审

    doi:10.1002/jbio.201300198

    authors: Bydlon TM,Nachabé R,Ramanujam N,Sterenborg HJ,Hendriks BH

    更新日期:2015-01-01 00:00:00

  • Whole-field macro- and micro-deformation characteristic of unbound water-loss in dentin hard tissue.

    abstract::High-resolution deformation measurements in a functionally graded hard tissue such as human dentin are essential to understand the unbound water-loss mediated changes and their role in its mechanical integrity. Yet a whole-field, 3-dimensional (3D) measurement and characterization of fully hydrated dentin in both macr...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201700368

    authors: Chen Z,Nadeau B,Yu K,Shao X,He X,Goh MC,Kishen A

    更新日期:2018-09-01 00:00:00

  • Intracellular localization and delivery of plasmid DNA by biodegradable microsphere-mediated femtosecond laser optoporation.

    abstract::Micro-/nanosphere-mediated femtosecond laser cell perforation is one of the high throughput technologies used for macro-molecule-delivery into multiple cells. We have demonstrated the delivery of plasmid-DNA/liposome complexes into cells using biodegradable polymer microspheres and a femtosecond laser and investigated...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201600323

    authors: Ishii A,Hiruta Y,Heinemann D,Heisterkamp A,Kanazawa H,Terakawa M

    更新日期:2017-12-01 00:00:00

  • In vitro photoacoustic spectroscopy of pulsatile blood flow: Probing the interrelationship between red blood cell aggregation and oxygen saturation.

    abstract::We investigate the optical wavelength dependence in quantitative photoacoustic (QPA) assessment of red blood cell (RBC) aggregation and oxygen saturation (sO2 ) during pulsatile blood flow. Experimentally, the pulsatile flow was imaged with a 700 to 900 nm laser using the VevoLAZR. Theoretically, the photoacoustic (PA...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201700300

    authors: Bok TH,Hysi E,Kolios MC

    更新日期:2018-08-01 00:00:00