Abstract:
:Molecular motors have been extensively studied over the past 25 years using a range of optical microscopy methods. The specific example of RNA polymerase, responsible for transcribing the information encoded in DNA into RNA, illustrates the strength of the convergence between these recent approaches and the vast store of data collected over the past half century using the more "classical" approaches of genetics, biochemistry, and structure elucidation.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Strick TRdoi
10.1002/jbio.200810030subject
Has Abstractpub_date
2008-09-01 00:00:00pages
269-79issue
4eissn
1864-063Xissn
1864-0648journal_volume
1pub_type
杂志文章abstract::Articular cartilage posesses unique material properties due to a complex depth-dependent composition of sub-components. Raman spectroscopy has proven valuable in quantifying this composition through cartilage cross-sections. However, cross-sectioning requires tissue destruction and is not practical in situ. In this wo...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000289
更新日期:2021-01-01 00:00:00
abstract::Dynamic follow-up of exogenous molecules permeation through the skin is one among many competing applications for confocal Raman microspectroscopy. Previous studies showed the feasibility of tracking actives through the skin; the next step should be recording in vivo kinetics. Thus, we conducted a study to evaluate th...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201300011
更新日期:2014-09-01 00:00:00
abstract::The purpose of this study was to evaluate the tomographic features of postrefractive surgery eyes. This was a retrospective evaluation of clinical data. Three patients with post-LASIK (laser-assisted in situ keratomileusis) and two patients with post-SMILE (small incision lenticule extraction) ectasia were imaged with...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800253
更新日期:2019-02-01 00:00:00
abstract::Cutaneous squamous cell carcinoma (cSCC) is a common skin cancer with metastatic potential. To reduce reoperations due to nonradical excision, there is a need to develop a technique for identification of tumor margins preoperatively. Photoacoustic (PA) imaging is a novel imaging technology that combines the strengths ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201960212
更新日期:2020-05-01 00:00:00
abstract::Ionizing radiation (IR) induces DNA damage and low-level laser therapy (LLLT) has been investigated to prevent or repair detrimental outcomes resulting from IR exposure. Few in vitro studies, however, explore the biological mechanisms underlying those LLLT benefits. Thus, in this work, fibroblasts and tumor cells are ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600107
更新日期:2016-12-01 00:00:00
abstract::We have investigated the ability of dual-frequency Coherent Antistokes Raman Scattering (D-CARS) micro-spectroscopy, based on femtosecond pulses (100 fs or 5 fs) spectrally focussed by glass dispersion, to distinguish the chemical composition of micron-sized lipid droplets consisting of different triglycerides types (...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200197
更新日期:2014-01-01 00:00:00
abstract::Low-power laser irradiation (LPLI) has been extensively employed to modulate inflammation in vitro and in vivo. Previous reports from our group indicated that LPLI might regulate glycemia in diabetic animals. Diabetes results in chronic hyperglycemia and therefore chronic inflammation by upregulation of inflammatory m...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600175
更新日期:2016-12-01 00:00:00
abstract::For the screening purposes urine is an especially attractive biofluid, since it offers easy and noninvasive sample collection and provides a snapshot of the whole metabolic status of the organism, which may change under different pathological conditions. Raman spectroscopy (RS) has the potential to monitor these chang...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900143
更新日期:2020-01-01 00:00:00
abstract::Stimulated Raman scattering (SRS) has been applied to unstained samples of articular cartilage enabling the investigation of living cells within fresh tissue. Hyperspectral SRS measurements over the CH vibrational region showed variations in protein and lipid content within the cells, pericellular matrix and interterr...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200213
更新日期:2013-10-01 00:00:00
abstract::In this work we present how to entirely remove the scattering ambiguity present in existing multiphoton multifocal systems. This is achieved through the development and implementation of single-element detection systems that incorporate high-speed photon-counting electronics. These systems can be used to image entire ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201100139
更新日期:2012-05-01 00:00:00
abstract::Acridine orange (AO) staining is used to diagnose DNA fragmentation status in sperm cells. Interferometric phase microscopy (IPM) is an optical imaging method based on digital holographic microscopy that provides quantitative morphological and refractive index imaging of cells in vitro without the need for staining. W...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800137
更新日期:2018-11-01 00:00:00
abstract::Surface-enhanced Raman scattering (SERS) spectroscopy possesses the most promising advantage of multiplex detection for biosensing applications, which is achieved due to the narrow 'fingerprint' Raman spectra from the analyte molecules. We developed an ultrasensitive platform for the multiplex detection of cancer biom...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201300084
更新日期:2014-11-01 00:00:00
abstract::Pancreatic islet isolation from donor pancreases is an essential step for the transplantation of insulin-secreting β-cells as a therapy to treat type 1 diabetes mellitus. This process however damages islet basement membranes, which can lead to islet dysfunction or death. Posttransplantation, islets are further stresse...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000375
更新日期:2020-12-01 00:00:00
abstract::Endoscopy has been widely used in biomedical imaging and integrated with various optical and acoustic imaging modalities. Photoacoustic imaging (PAI), one of the fastest growing biomedical imaging modalities, is a noninvasive and nonionizing method that owns rich optical contrast, deep acoustic penetration depth, mult...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.202000217
更新日期:2020-12-01 00:00:00
abstract::Infrared spectra obtained from cell or tissue specimen have commonly been observed to involve a significant degree of scattering effects, often Mie scattering, which probably overshadows biochemically relevant spectral information by a nonlinear, nonadditive spectral component in Fourier transform infrared (FTIR) spec...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000385
更新日期:2020-12-08 00:00:00
abstract::The authors have developed a tomographic diffractive microscope that combines microholography with illumination from an angular synthetic aperture. It images specimens relative to their complex index of refraction distribution (index and absorption) and permits imaging of unlabelled specimens, with high lateral resolu...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.200900094
更新日期:2010-07-01 00:00:00
abstract::Microfluidics and photonics come together to form a field commonly referred to as 'optofluidics'. Flow cytometry provides the field with a technology base from which both microfluidic and photonic components be developed and integrated into a useful device. This article reviews some of the more recent developments to ...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.200810018
更新日期:2008-10-01 00:00:00
abstract::A robust method is presented for evaluating the diffusion properties of chemicals in ex vivo biological tissues. Using this method that relies only on thickness and collimated transmittance measurements, the diffusion properties of glycerol, fructose, polypropylene glycol and water in muscle tissues were evaluated. Am...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800333
更新日期:2019-04-01 00:00:00
abstract::Photodynamic inactivation (PDI) is a promising alternative for combating infections caused by antimicrobial resistant bacteria. Pneumonias are among the most worrisome infections because of their high-mortality rate. Previous studies have demonstrated the feasibility of using PDI with extracorporeal light to treat pne...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000176
更新日期:2020-10-01 00:00:00
abstract::We fabricated composite nanoparticles consisting of a plasmonic core (gold nanorods or gold-silver nanocages) and a hematoporphyrin-doped silica shell. The dual photodynamic and photothermal activities of such nanoparticles against Staphylococcus aureus 209 P were studied and compared with the activities of reference ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200079
更新日期:2013-04-01 00:00:00
abstract::Near-infrared light allows measuring tissue oxygenation. These measurements relay on oxygenation-dependent absorption spectral changes. However, the tissue scattering, which is also spectral dependent, introduces an intrinsic error. Most methods focus on the volume reflectance from a semi-infinite sample. We have prop...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700208
更新日期:2018-04-01 00:00:00
abstract::A Monte Carlo simulation was adapted to specify a skin model with pigmented epidermis, dermis with low baseline blood content, and vessels of a vascular lesion with an average added blood volume fraction of 5%. In the study, the lesion vessel diameters were increased and the number of lesion vessels decreased, such th...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.200900085
更新日期:2010-01-01 00:00:00
abstract::Currently, optical coherence tomography (OCT), is not capable of obtaining molecular information often crucial for identification of disease. To enable molecular imaging with OCT, we have further developed a technique that harnesses transient changes in light absorption in the sample to garner molecular information. A...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201300119
更新日期:2015-01-01 00:00:00
abstract::The capabilities of cross-polarization optical coherence tomography (CP OCT) for early bladder-cancer detection are assessed in statistical study and compared with the traditional OCT. Unlike the traditional OCT that demonstrates images only in copolarization, CP OCT acquires images in cross-polarization and copolariz...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201000088
更新日期:2011-08-01 00:00:00
abstract::The ability to resolve multiple fluorescent emissions from different biological targets in video rate applications, such as endoscopy and intraoperative imaging, has traditionally been limited by the use of filter-based imaging systems. Hyperspectral imaging (HSI) facilitates the detection of both spatial and spectral...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600304
更新日期:2017-06-01 00:00:00
abstract::A simple scheme for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy is based on the spectral focusing of ultrafast-oscillator-derived pump/probe light and synchronous photonic crystal fiber (PCF) fiber-generated broadband Stokes light. To date, such schemes allowed rapid hyperspectral imaging throug...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200171
更新日期:2014-01-01 00:00:00
abstract::Diffuse reflectance spectroscopy is a rapidly growing technology in the biophotonics community where it has shown promise in its ability to classify different tissues. In the steady-state domain a wide spectrum of clinical applications is supported with this technology ranging from diagnostic to guided interventions. ...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.201300198
更新日期:2015-01-01 00:00:00
abstract::High-resolution deformation measurements in a functionally graded hard tissue such as human dentin are essential to understand the unbound water-loss mediated changes and their role in its mechanical integrity. Yet a whole-field, 3-dimensional (3D) measurement and characterization of fully hydrated dentin in both macr...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700368
更新日期:2018-09-01 00:00:00
abstract::Micro-/nanosphere-mediated femtosecond laser cell perforation is one of the high throughput technologies used for macro-molecule-delivery into multiple cells. We have demonstrated the delivery of plasmid-DNA/liposome complexes into cells using biodegradable polymer microspheres and a femtosecond laser and investigated...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600323
更新日期:2017-12-01 00:00:00
abstract::We investigate the optical wavelength dependence in quantitative photoacoustic (QPA) assessment of red blood cell (RBC) aggregation and oxygen saturation (sO2 ) during pulsatile blood flow. Experimentally, the pulsatile flow was imaged with a 700 to 900 nm laser using the VevoLAZR. Theoretically, the photoacoustic (PA...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700300
更新日期:2018-08-01 00:00:00