Spatial mapping of affinity changes for the integrin LFA-1 during cell migration using clusters identified based on local density.

Abstract:

:Localization microscopy methods like Stochastic Optical Reconstruction Microscopy (STORM) are very well suited for exploring clustering of proteins, as the data inherently provide a list of molecular coordinates. Here we use state-of-art cluster analysis algorithms (DBSCAN) to explore the clustering behaviour of different affinity forms of the integrin LFA-1. It has been suggested that LFA-1 may form clusters, in order to increase the avidity to ICAM-1. However, this hypothesis still seems to be controversial. In this study, we found, variations in clustering behaviour among the different affinity forms of LFA-1 in migrating T-cells. We found that panLFA-1 is located in clusters throughout the polarised cell on ICAM-1, with an increased density of molecules and clusters in the mid area and rear of the cell, whereas the intermediate and high affinity form of LFA-1 showed an increased number in the mid area of a migrating cell and the high affinity form of LFA-1 in the front and rear. Together, these data suggest that, in addition to LFA-1 conformation, protein clustering might play a role in controlling cell-substrate adhesion on ICAM-1.By applying the cluster analysis algorithm DBSCAN to localization microscopy data, integrin clusters could be identified and different cluster parameters could be quantified.

journal_name

J Biophotonics

journal_title

Journal of biophotonics

authors

Persson H,Potrzebowski W,Potrzebowska K,Svensson LM

doi

10.1002/jbio.201800080

subject

Has Abstract

pub_date

2019-03-01 00:00:00

pages

e201800080

issue

3

eissn

1864-063X

issn

1864-0648

journal_volume

12

pub_type

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