Effect of tissue staining in quantitative phase imaging.

Abstract:

:Quantitative phase imaging (QPI) is an emerging modality, which enables the identification of abnormalities in tissue based on optical properties. QPI can be applied to any biological specimen due to its label-free imaging capability, but its use in stained tissue is unclear. Here, we study the variability of QPI with the staining dye. Several tissues such as brain, heart and lung were stained with hematoxylin and eosin, and their optical properties compared at 550 and 730 nm. Our results showed that phase and scattering coefficients varied when QPI was used at the absorption wavelength of the staining dye. We also found that the variation of optical properties was dependent on tissue morphology.

journal_name

J Biophotonics

journal_title

Journal of biophotonics

authors

Ban S,Min E,Ahn Y,Popescu G,Jung W

doi

10.1002/jbio.201700402

subject

Has Abstract

pub_date

2018-08-01 00:00:00

pages

e201700402

issue

8

eissn

1864-063X

issn

1864-0648

journal_volume

11

pub_type

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