Abstract:
:Quantitative phase imaging (QPI) is an emerging modality, which enables the identification of abnormalities in tissue based on optical properties. QPI can be applied to any biological specimen due to its label-free imaging capability, but its use in stained tissue is unclear. Here, we study the variability of QPI with the staining dye. Several tissues such as brain, heart and lung were stained with hematoxylin and eosin, and their optical properties compared at 550 and 730 nm. Our results showed that phase and scattering coefficients varied when QPI was used at the absorption wavelength of the staining dye. We also found that the variation of optical properties was dependent on tissue morphology.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Ban S,Min E,Ahn Y,Popescu G,Jung Wdoi
10.1002/jbio.201700402subject
Has Abstractpub_date
2018-08-01 00:00:00pages
e201700402issue
8eissn
1864-063Xissn
1864-0648journal_volume
11pub_type
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