Hyperspectral optical coherence tomography for in vivo visualization of melanin in the retinal pigment epithelium.

Abstract:

:Previous studies for melanin visualization in the retinal pigment epithelium (RPE) have exploited either its absorption properties (using photoacoustic tomography or photothermal optical coherence tomography [OCT]) or its depolarization properties (using polarization sensitive OCT). However, these methods are only suitable when the melanin concentration is sufficiently high. In this work, we present the concept of hyperspectral OCT for melanin visualization in the RPE when the concentration is low. Based on white light OCT, a hyperspectral stack of 27 wavelengths (440-700 nm) was created in post-processing for each depth-resolved image. Owing to the size and shape of the melanin granules in the RPE, the variations in backscattering coefficient as a function of wavelength could be identified-a result which is to be expected from Mie theory. This effect was successfully identified both in eumelanin-containing phantoms and in vivo in the low-concentration Brown Norway rat RPE.

journal_name

J Biophotonics

journal_title

Journal of biophotonics

authors

Harper DJ,Konegger T,Augustin M,Schützenberger K,Eugui P,Lichtenegger A,Merkle CW,Hitzenberger CK,Glösmann M,Baumann B

doi

10.1002/jbio.201900153

subject

Has Abstract

pub_date

2019-12-01 00:00:00

pages

e201900153

issue

12

eissn

1864-063X

issn

1864-0648

journal_volume

12

pub_type

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