Sensitive multiplex detection of serological liver cancer biomarkers using SERS-active photonic crystal fiber probe.


:Surface-enhanced Raman scattering (SERS) spectroscopy possesses the most promising advantage of multiplex detection for biosensing applications, which is achieved due to the narrow 'fingerprint' Raman spectra from the analyte molecules. We developed an ultrasensitive platform for the multiplex detection of cancer biomarkers by combining the SERS technique with a hollow-core photonic crystal fiber (HCPCF). Axially aligned air channels inside the HCPCF provide an excellent platform for optical sensing using SERS. In addition to the flexibility of optical fibers, HCPCF provides better light confinement and a larger interaction length for the guided light and the analyte, resulting in an improvement in sensitivity to detect low concentrations of bioanalytes in extremely low sample volumes. Herein, for the first time, we demonstrate the sensitive multiplex detection of biomarkers immobilized inside the HCPCF using antibody-conjugated SERS-active nanoparticles (SERS nanotags). As a proof-of-concept for targeted multiplex detection, initially we carried out the sensing of epidermal growth factor receptor (EGFR) biomarker in oral squamous carcinoma cell lysate using three different SERS nanotags. Subsequently, we also achieved simultaneous detection of hepatocellular carcinoma (HCC) biomarkers-alpha fetoprotein (AFP) and alpha-1-antitrypsin (A1AT) secreted in the supernatant from Hep3b cancer cell line. Using a SERS-HCPCF sensing platform, we could successfully demonstrate the multiplex detection in an extremely low sample volume of ∼20 nL. In future, this study may lead to sensitive biosensing platform for the low concentration detection of biomarkers in an extremely low sample volume of body fluids to achieve early diagnosis of multiple diseases. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).


J Biophotonics


Journal of biophotonics


Dinish US,Balasundaram G,Chang YT,Olivo M




Has Abstract


2014-11-01 00:00:00












  • An automatic diagnostic system of coronary artery lesions in Kawasaki disease using intravascular optical coherence tomography imaging.

    abstract::Intravascular optical coherence tomography (IV-OCT) is a light-based imaging modality with high resolution, which employs near-infrared light to provide tomographic intracoronary images. Morbidity caused by coronary heart disease is a substantial cause of acute coronary syndrome and sudden cardiac death. The most comm...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Abdolmanafi A,Cheriet F,Duong L,Ibrahim R,Dahdah N

    更新日期:2020-01-01 00:00:00

  • Optical clearing for multiscale biological tissues.

    abstract::Three-dimensional reconstruction of tissue structures is essential for biomedical research. The development of light microscopes and various fluorescent labeling techniques provides powerful tools for this motivation. However, optical imaging depth suffers from strong light scattering due to inherent heterogeneity of ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Yu T,Qi Y,Gong H,Luo Q,Zhu D

    更新日期:2018-02-01 00:00:00

  • Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes.

    abstract::An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probes-upon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensors-is reported. To this end, high sensitivity photonic sensors based on silicon photonic bandgap (PBG) structur...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Ruiz-Tórtola Á,Prats-Quílez F,González-Lucas D,Bañuls MJ,Maquieira Á,Wheeler G,Dalmay T,Griol A,Hurtado J,Bohlmann H,Götzen R,García-Rupérez J

    更新日期:2018-10-01 00:00:00

  • Characterization of the optical properties of color pastes for the design of optical phantoms mimicking biological tissue.

    abstract::Clinicians need a way to rapidly and reliably test the correct functioning of near-infrared spectroscopy (NIRS)-based oximeters. Therefore, optical phantoms for quality assessment of NIRS oximeters are needed. The fabrication of such phantoms that mimic the optical properties of biological tissue in the NIR range repr...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Tomm N,Ahnen L,Isler H,Kleiser S,Karen T,Ostojic D,Wolf M,Scholkmann F

    更新日期:2019-04-01 00:00:00

  • A robust ex vivo method to evaluate the diffusion properties of agents in biological tissues.

    abstract::A robust method is presented for evaluating the diffusion properties of chemicals in ex vivo biological tissues. Using this method that relies only on thickness and collimated transmittance measurements, the diffusion properties of glycerol, fructose, polypropylene glycol and water in muscle tissues were evaluated. Am...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Carneiro I,Carvalho S,Henrique R,Oliveira LM,Tuchin VV

    更新日期:2019-04-01 00:00:00

  • Measurement of cartilage sub-component distributions through the surface by Raman spectroscopy-based multivariate analysis.

    abstract::Articular cartilage posesses unique material properties due to a complex depth-dependent composition of sub-components. Raman spectroscopy has proven valuable in quantifying this composition through cartilage cross-sections. However, cross-sectioning requires tissue destruction and is not practical in situ. In this wo...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Mason D,Murugkar S,Speirs AD

    更新日期:2021-01-01 00:00:00

  • Diffractive corneal inlay for presbyopia.

    abstract::A conceptually new type of corneal inlays for a customized treatment of presbyopia is presented. The diffractive inlay consists on a small aperture disc having an array of micro-holes distributed inside the open zones of a Fresnel zone plate. In this way, the central hole of the disc lets pass the zero order diffracti...

    journal_title:Journal of biophotonics

    pub_type: 信件


    authors: Furlan WD,García-Delpech S,Udaondo P,Remón L,Ferrando V,Monsoriu JA

    更新日期:2017-09-01 00:00:00

  • Three-dimensional (3D) backward and forward second harmonic generation (SHG) microscopy of biological tissues.

    abstract::In this work we aim to show how it is possible to exploit the second harmonic generation (SHG) signal for producing multimodal microscopic images of biological tissues. SHG microscopy constitutes an important tool for high-resolution, high-contrast, three-dimensional studies of live cell and tissue architectures. The ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Bianchini P,Diaspro A

    更新日期:2008-12-01 00:00:00

  • Noninvasive in vivo glucose detection in human finger interstitial fluid using wavelength-modulated differential photothermal radiometry.

    abstract::We present a noninvasive and noncontacting biosensor using Wavelength Modulated Differential Photothermal Radiometry (WM-DPTR) to monitor blood glucose concentration (BGC) through interstitial fluid (ISF) probing in human middle fingers. WM-DPTR works in the interference-free mid-infrared range with differential wavel...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Guo X,Zhang D,Shojaei-Asanjan K,Sivagurunathan K,Melnikov A,Song P,Mandelis A

    更新日期:2019-07-01 00:00:00

  • Biocompatible astaxanthin as novel contrast agent for biomedical imaging.

    abstract::Photoacoustic imaging (PAI) is a hybrid imaging modality with high resolution and sensitivity that can be beneficial for cancer staging. Due to insufficient endogenous photoacoustic (PA) contrast, the development of exogenous agents is critical in targeting cancerous tumors. The current study demonstrates the feasibil...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Nguyen VP,Park S,Oh J,Wook Kang H

    更新日期:2017-08-01 00:00:00

  • Detecting hemodynamic changes in the foot vessels of diabetic patients by photoacoustic tomography.

    abstract::Limb perfusion monitoring is critical for diabetes mellitus (DM) patients as they are vulnerable to vascular complications due to prolonged hyperglycemia. However, current clinical approaches are ineffective in vascular imaging and in assessing vascular function in lower limbs. In this work, a concave ultrasound trans...

    journal_title:Journal of biophotonics

    pub_type: 信件


    authors: Yang J,Zhang G,Shang Q,Wu M,Huang L,Jiang H

    更新日期:2020-08-01 00:00:00

  • Analysis of basidiomycete pigments in situ by Raman spectroscopy.

    abstract::Basidiomycetes, that is, mushroom-type fungi, are known to produce pigments in response to environmental impacts. As antioxidants with a high level of unsaturation, these compounds can neutralize highly oxidative species. In the event of close contact with other microbes, the enzymatically controlled pigment productio...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Tauber JP,Matthäus C,Lenz C,Hoffmeister D,Popp J

    更新日期:2018-06-01 00:00:00

  • Low-power laser irradiation in salivary glands reduces glycemia in streptozotocin-induced diabetic female rats.

    abstract::Low-power laser irradiation (LPLI) has been extensively employed to modulate inflammation in vitro and in vivo. Previous reports from our group indicated that LPLI might regulate glycemia in diabetic animals. Diabetes results in chronic hyperglycemia and therefore chronic inflammation by upregulation of inflammatory m...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Fukuoka CY,Torres Schröter G,Nicolau J,Simões A

    更新日期:2016-12-01 00:00:00

  • Stereotaxic endoscopy for the ocular imaging of awake, freely moving animal models.

    abstract::Stereotaxic instruments are increasingly used in research animals for the study of disease, but typically require restraints and anesthetic procedures. A stereotaxic head mount that enables imaging of the anterior chamber of the eye in alert and freely mobile mice is presented in this study. The head mount is fitted b...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Paulson B,Lee S,Jue M,Lee K,Lee S,Kim GB,Moon Y,Lee JY,Kim N,Kim JK

    更新日期:2020-05-01 00:00:00

  • Analgesic effect of Photobiomodulation Therapy: An in vitro and in vivo study.

    abstract::Laser therapy, also known as Photobiomodulation (PBM) is indicated to reduce pain associated with different pathologies and applied using protocols that vary in wavelength, irradiance and fluence. Its mechanisms of action are still unclear and possibly able to directly impact on pain transmission, reducing nociceptor ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Zupin L,Ottaviani G,Rupel K,Biasotto M,Zacchigna S,Crovella S,Celsi F

    更新日期:2019-10-01 00:00:00

  • Longtime driving induced cerebral hemodynamic elevation and behavior degradation as assessed by functional near-infrared spectroscopy and a voluntary attention test.

    abstract::Drowsy driving contributes to ~20% of all traffic accidents worldwide. Onsite monitoring the mental condition of a driver and forewarning may be a preventive solution to reduce occurrence of drowsiness and potential accidents. Functional near-infrared spectroscopy (fNIRS) has been successfully utilized in hemodynamics...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Li T,Lin Y,Gao Y,Zhong F

    更新日期:2018-12-01 00:00:00

  • Artifact-free objective-type multicolor total internal reflection fluorescence microscopy with light-emitting diode light sources-Part I.

    abstract::Total internal reflection fluorescence excitation (TIRF) microscopy allows the selective observation of fluorescent molecules in immediate proximity to an interface between different refractive indices. Objective-type or prism-less TIRF excitation is typically achieved with laser light sources. We here propose a simpl...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Kogel A,Kalwa H,Urban N,Schaefer M

    更新日期:2019-11-01 00:00:00

  • Prognostic analysis of amyotrophic lateral sclerosis based on clinical features and plasma surface-enhanced Raman spectroscopy.

    abstract::Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease with a wide range of survival times. We aimed to explore prognostic factors related to short survival based on clinical features and plasma metabolic signatures using surface-enhanced Raman spectroscopy (SERS). One hundred and thirty-eight sporad...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Zhang QJ,Chen Y,Zou XH,Hu W,Lin XL,Feng SY,Chen F,Xu LQ,Chen WJ,Wang N

    更新日期:2019-08-01 00:00:00

  • Imaging engineered tissues using structural and functional optical coherence tomography.

    abstract::As the field of tissue engineering evolves, there will be an increasingly important need to visualize and track the complex dynamic changes that occur within three-dimensional constructs. Optical coherence tomography (OCT), as an emerging imaging technology applied to biological materials, offers a number of significa...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Liang X,Graf BW,Boppart SA

    更新日期:2009-11-01 00:00:00

  • Which wavelength is optimal for transcranial low-level laser stimulation?

    abstract::One of the challenges in transcranial low-level laser therapy (LLLT) is to optimally choose illumination parameters, such as wavelength. However, there is sparse study on the wavelengths comparison especially on human transcranial LLLT. Here, we employed Monte Carlo modeling and visible human phantom to compute the pe...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Wang P,Li T

    更新日期:2019-02-01 00:00:00

  • Fingerprint-to-CH stretch continuously tunable high spectral resolution stimulated Raman scattering microscope.

    abstract::Stimulated Raman scattering (SRS) microscopy is a label-free method generating images based on chemical contrast within samples, and has already shown its great potential for high-sensitivity and fast imaging of biological specimens. The capability of SRS to collect molecular vibrational signatures in bio-samples, cou...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Laptenok SP,Rajamanickam VP,Genchi L,Monfort T,Lee Y,Patel II,Bertoncini A,Liberale C

    更新日期:2019-09-01 00:00:00

  • Chromophore based analyses of steady-state diffuse reflectance spectroscopy: current status and perspectives for clinical adoption.

    abstract::Diffuse reflectance spectroscopy is a rapidly growing technology in the biophotonics community where it has shown promise in its ability to classify different tissues. In the steady-state domain a wide spectrum of clinical applications is supported with this technology ranging from diagnostic to guided interventions. ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Bydlon TM,Nachabé R,Ramanujam N,Sterenborg HJ,Hendriks BH

    更新日期:2015-01-01 00:00:00

  • Pseudo-real-time retinal layer segmentation for high-resolution adaptive optics optical coherence tomography.

    abstract::We present a pseudo-real-time retinal layer segmentation for high-resolution Sensorless Adaptive Optics-Optical Coherence Tomography (SAO-OCT). Our pseudo-real-time segmentation method is based on Dijkstra's algorithm that uses the intensity of pixels and the vertical gradient of the image to find the minimum cost in ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Janpongsri W,Huang J,Ng R,Wahl DJ,Sarunic MV,Jian Y

    更新日期:2020-08-01 00:00:00

  • Hybrid method of strain estimation in optical coherence elastography using combined sub-wavelength phase measurements and supra-pixel displacement tracking.

    abstract::A novel hybrid method which combines sub-wavelength-scale phase measurements and pixel-scale displacement tracking for robust strain mapping in compressional optical coherence elastography is proposed. Unlike majority of OCE methods it does not rely on initial reconstruction of displacements and does not suffer from t...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Zaitsev VY,Matveyev AL,Matveev LA,Gelikonov GV,Gubarkova EV,Gladkova ND,Vitkin A

    更新日期:2016-05-01 00:00:00

  • Determination of the metabolic index using the fluorescence lifetime of free and bound nicotinamide adenine dinucleotide using the phasor approach.

    abstract::The fluorescence lifetime of nicotinamide adenine dinucleotide (NADH) is commonly used in conjunction with the phasor approach as a molecular biomarker to provide information on cellular metabolism of autofluorescence imaging of cells and tissue. However, in the phasor approach, the bound and free lifetime defining th...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Ranjit S,Malacrida L,Stakic M,Gratton E

    更新日期:2019-11-01 00:00:00

  • Microfluidics and photonics for Bio-System-on-a-Chip: a review of advancements in technology towards a microfluidic flow cytometry chip.

    abstract::Microfluidics and photonics come together to form a field commonly referred to as 'optofluidics'. Flow cytometry provides the field with a technology base from which both microfluidic and photonic components be developed and integrated into a useful device. This article reviews some of the more recent developments to ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Godin J,Chen CH,Cho SH,Qiao W,Tsai F,Lo YH

    更新日期:2008-10-01 00:00:00

  • Unique spectral signature of human cutaneous squamous cell carcinoma by photoacoustic imaging.

    abstract::Cutaneous squamous cell carcinoma (cSCC) is a common skin cancer with metastatic potential. To reduce reoperations due to nonradical excision, there is a need to develop a technique for identification of tumor margins preoperatively. Photoacoustic (PA) imaging is a novel imaging technology that combines the strengths ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Hult J,Dahlstrand U,Merdasa A,Wickerström K,Chakari R,Persson B,Cinthio M,Erlöv T,Albinsson J,Gesslein B,Sheikh R,Malmsjö M

    更新日期:2020-05-01 00:00:00

  • Treatment of tumor in lymph nodes using near-infrared laser light-activated thermosensitive liposome-encapsulated doxorubicin and gold nanorods.

    abstract::Tumor metastasis to lymph nodes is an important contributory factor for cancer-related deaths despite recent developments in cancer therapy. In this study, we demonstrate that tumor in the proper axillary lymph node (PALN) of the mouse can be treated by the application of external laser light to trigger the unloading ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Matsuki D,Adewale O,Horie S,Okajima J,Komiya A,Oluwafemi O,Maruyama S,Mori S,Kodama T

    更新日期:2017-12-01 00:00:00

  • Autofocusing technologies for whole slide imaging and automated microscopy.

    abstract::Whole slide imaging (WSI) has moved digital pathology closer to diagnostic practice in recent years. Due to the inherent tissue topography variability, accurate autofocusing remains a critical challenge for WSI and automated microscopy systems. The traditional focus map surveying method is limited in its ability to ac...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审


    authors: Bian Z,Guo C,Jiang S,Zhu J,Wang R,Song P,Zhang Z,Hoshino K,Zheng G

    更新日期:2020-12-01 00:00:00

  • N2NSR-OCT: Simultaneous denoising and super-resolution in optical coherence tomography images using semisupervised deep learning.

    abstract::Optical coherence tomography (OCT) imaging shows a significant potential in clinical routines due to its noninvasive property. However, the quality of OCT images is generally limited by inherent speckle noise of OCT imaging and low sampling rate. To obtain high signal-to-noise ratio (SNR) and high-resolution (HR) OCT ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章


    authors: Qiu B,You Y,Huang Z,Meng X,Jiang Z,Zhou C,Liu G,Yang K,Ren Q,Lu Y

    更新日期:2021-01-01 00:00:00