Abstract:
:An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probes-upon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensors-is reported. To this end, high sensitivity photonic sensors based on silicon photonic bandgap (PBG) structures were used, where the MB probes were immobilized via their 5' termination. Those MBs incorporate a biotin moiety close to their 3' termination in order to selectively bind a streptavidin molecule to them. The different photonic sensing responses obtained toward the target oligonucleotide detection, when the streptavidin molecule was bound to the MB probes or not, demonstrate the conformational change suffered by the MB upon hybridization, which promotes the displacement of the streptavidin molecule away from the surface of the photonic sensing structure.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Ruiz-Tórtola Á,Prats-Quílez F,González-Lucas D,Bañuls MJ,Maquieira Á,Wheeler G,Dalmay T,Griol A,Hurtado J,Bohlmann H,Götzen R,García-Rupérez Jdoi
10.1002/jbio.201800030subject
Has Abstractpub_date
2018-10-01 00:00:00pages
e201800030issue
10eissn
1864-063Xissn
1864-0648journal_volume
11pub_type
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