Abstract:
:Achieving a maximal safe extent of resection during brain tumor surgery is the goal for improved patient prognosis. Fluorescence-guided neurosurgery using 5-aminolevulinic acid (5-ALA) induced protoporphyrin IX has thereby become a valuable tool enabling a high frequency of complete resections and a prolonged progression-free survival in glioblastoma patients. We present a widefield fluorescence lifetime imaging device with 250 mm working distance, working under similar conditions such as surgical microscopes based on a time-of-flight dual tap CMOS camera. In contrast to intensity-based fluorescence imaging, our method is invariant to light scattering and absorption while being sensitive to the molecular composition of the tissue. We evaluate the feasibility of lifetime imaging of protoporphyrin IX using our system to analyze brain tumor phantoms and fresh 5-ALA-labeled human tissue samples. The results demonstrate the potential of our lifetime sensing device to go beyond the limitation of current intensity-based fluorescence-guided neurosurgery.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Erkkilä MT,Bauer B,Hecker-Denschlag N,Madera Medina MJ,Leitgeb RA,Unterhuber A,Gesperger J,Roetzer T,Hauger C,Drexler W,Widhalm G,Andreana Mdoi
10.1002/jbio.201800378subject
Has Abstractpub_date
2019-06-01 00:00:00pages
e201800378issue
6eissn
1864-063Xissn
1864-0648journal_volume
12pub_type
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