Abstract:
:Infrared spectra obtained from cell or tissue specimen have commonly been observed to involve a significant degree of scattering effects, often Mie scattering, which probably overshadows biochemically relevant spectral information by a nonlinear, nonadditive spectral component in Fourier transform infrared (FTIR) spectroscopic measurements. Correspondingly, many successful machine learning approaches for FTIR spectra have relied on preprocessing procedures that computationally remove the scattering components from an infrared spectrum. We propose an approach to approximate this complex preprocessing function using deep neural networks. As we demonstrate, the resulting model is not just several orders of magnitudes faster, which is important for real-time clinical applications, but also generalizes strongly across different tissue types. Using Bayesian machine learning approaches, our approach unveils model uncertainty that coincides with a band shift in the amide I region that occurs when scattering is removed computationally based on an established physical model. Furthermore, our proposed method overcomes the trade-off between computation time and the corrected spectrum being biased towards an artificial reference spectrum.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Raulf AP,Butke J,Menzen L,Küpper C,Großerueschkamp F,Gerwert K,Mosig Adoi
10.1002/jbio.202000385subject
Has Abstractpub_date
2020-12-08 00:00:00pages
e202000385eissn
1864-063Xissn
1864-0648pub_type
杂志文章abstract::Dynamic follow-up of exogenous molecules permeation through the skin is one among many competing applications for confocal Raman microspectroscopy. Previous studies showed the feasibility of tracking actives through the skin; the next step should be recording in vivo kinetics. Thus, we conducted a study to evaluate th...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201300011
更新日期:2014-09-01 00:00:00
abstract::Surface-enhanced Raman scattering (SERS) is highly sensitive and label-free analytical technique based on Raman spectroscopy aided by field-multiplying plasmonic nanostructures. We report the use of SERS measurements of patient urine in conjunction with biostatistical algorithms to assess the treatment response of pro...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000275
更新日期:2021-01-01 00:00:00
abstract::Limb perfusion monitoring is critical for diabetes mellitus (DM) patients as they are vulnerable to vascular complications due to prolonged hyperglycemia. However, current clinical approaches are ineffective in vascular imaging and in assessing vascular function in lower limbs. In this work, a concave ultrasound trans...
journal_title:Journal of biophotonics
pub_type: 信件
doi:10.1002/jbio.202000011
更新日期:2020-08-01 00:00:00
abstract::Three-dimensional reconstruction of tissue structures is essential for biomedical research. The development of light microscopes and various fluorescent labeling techniques provides powerful tools for this motivation. However, optical imaging depth suffers from strong light scattering due to inherent heterogeneity of ...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.201700187
更新日期:2018-02-01 00:00:00
abstract::Single-molecule localisation based super-resolution fluorescence imaging produces maps of the coordinates of fluorescent molecules in a region of interest. Cluster analysis algorithms provide information concerning the clustering characteristics of these molecules, often through the generation of cluster heat maps bas...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201400127
更新日期:2015-11-01 00:00:00
abstract::Fluctuations in brain water content has attracted increasing interest, particularly as regards studies of the glymphatic system, which is connected with the complex organization of dural lymphatic vessels, responsible for cleaning tissue. Disturbances of glymphatic circulation are associated with several brain disorde...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700123
更新日期:2018-08-01 00:00:00
abstract::Semiconductor nanoparticles (NPs) became important and wide-used tool for cell imaging because of their unique remarkable properties. Nevertheless, all previous investigations in this area were done on proliferating cells. For the first time, this work demonstrates strong influence of cell active proliferation/contact...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200066
更新日期:2013-03-01 00:00:00
abstract::We suggest to use a combination of optical tweezers and single-image quantitative differential interference contrast (DIC) emulated by a spatial light modulator (SLM) to study physiological shape changes in thrombocytes after activation and demonstrate the effectiveness of this system for the given task. A specially d...
journal_title:Journal of biophotonics
pub_type: 社论
doi:10.1002/jbio.201100010
更新日期:2011-09-01 00:00:00
abstract::In fluctuation-based optical nanoscopy, investigating high-density labeled subcellular structures with high fidelity has been a significant challenge. In this study, based on super-resolution radial fluctuation (SRRF) microscopy, the joint tagging (JT) strategy is employed to enable fast high-density nanoscopic imagin...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800020
更新日期:2018-09-01 00:00:00
abstract::The aim of the present study is to optimize parameters for inhibiting neuronal activity safely and investigating thermal inhibition of rat cortex neural networks in vitro by continuous infrared (IR) laser. Rat cortex neurons were cultured on multi-electrode arrays until neural networks were formed with spontaneous neu...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800403
更新日期:2019-07-01 00:00:00
abstract::Photodynamic inactivation (PDI) is a promising alternative for combating infections caused by antimicrobial resistant bacteria. Pneumonias are among the most worrisome infections because of their high-mortality rate. Previous studies have demonstrated the feasibility of using PDI with extracorporeal light to treat pne...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000176
更新日期:2020-10-01 00:00:00
abstract::Total internal reflection fluorescence excitation (TIRF) microscopy allows the selective observation of fluorescent molecules in immediate proximity to an interface between different refractive indices. Objective-type or prism-less TIRF excitation is typically achieved with laser light sources. We here propose a simpl...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900033
更新日期:2019-11-01 00:00:00
abstract::Achieving a maximal safe extent of resection during brain tumor surgery is the goal for improved patient prognosis. Fluorescence-guided neurosurgery using 5-aminolevulinic acid (5-ALA) induced protoporphyrin IX has thereby become a valuable tool enabling a high frequency of complete resections and a prolonged progress...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800378
更新日期:2019-06-01 00:00:00
abstract::Methods of nonlinear optics provide a vast arsenal of tools for label-free brain imaging, offering a unique combination of chemical specificity, the ability to detect fine morphological features, and an unprecedentedly high, subdiffraction spatial resolution. While these techniques provide a rapidly growing platform f...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800432
更新日期:2019-11-01 00:00:00
abstract::Atherosclerosis (AS), the leading cause of morbidity and mortality in cardiovascular disease, needs an early detection for treatment and prevention of fatal events. Here, for the first time, we applied gold nanorods (GNRs)-assisted diffusion reflection (DR), a noninvasive technique for in vivo detection of AS in a hig...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800218
更新日期:2019-01-01 00:00:00
abstract::A plastic optical fibre biosensor based on surface plasmon resonance for the detection of C-reactive protein (CRP) in serum is proposed. The biosensor was integrated into a home-made thermo-stabilized microfluidic system that allows avoiding any thermal and/or mechanical fluctuation and maintaining the best stable con...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201500315
更新日期:2016-10-01 00:00:00
abstract::The class of PEGylated emulsifiers finds broad application in the pharmaceutical and cosmetic industry. We target on one of the categories of polyethylene glycol (PEG) alkyl ethers with different lipophilic and hydrophilic chain length and aim to examine their effects on the skin comprehensively. In this study, we emp...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000286
更新日期:2020-12-01 00:00:00
abstract::The above article from the Journal of Biophotonics, published online on 9 January 2018 in Wiley Online Library as Early View (https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201700354), and in Volume 11, e201700354, has been retracted by agreement between the authors (Hong Zhong, Lin Lu, Yuanhao Wang, Hongxing Ya...
journal_title:Journal of biophotonics
pub_type: 杂志文章,撤回出版物
doi:10.1002/jbio.201890133
更新日期:2020-04-01 00:00:00
abstract::Highly emissive europium complexes with specific endoplasmic reticulum localization potential includes several advantages such as fast uptake, long resident lifetime, low dosage requirement, low cytotoxicity and highly emissive two-photon induced f-f emission imaging. ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.200910052
更新日期:2009-12-01 00:00:00
abstract::Myelin is a key component of the peripheral nervous system, whose structure anomaly in the digital skin is implicated in neuropathy. Here we demonstrate an in vivo labeling and imaging technique, capable of visualizing myelin sheaths deep in the mouse digital skin. Through material characterization, we verify that 3-p...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000321
更新日期:2020-12-01 00:00:00
abstract::In this review we provide a description of the basic concepts and paradigms currently constituting the foundations of adult stem cell biology, and discuss the role that live imaging techniques have in the development of the field. We focus on live imaging of hematopoietic stem cells (HSCs) as the basic biology and cli...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.200910072
更新日期:2009-11-01 00:00:00
abstract::Several studies have demonstrated the potential of cross-polarization optical coherence tomography (CP-OCT) to quantify the severity of early caries lesions (tooth decay) on tooth surfaces. The purpose of this study is to show that 2D images of the lesion depth and the integrated reflectivity can be used to accurately...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201300137
更新日期:2015-01-01 00:00:00
abstract::Malaria is a life-threatening infectious blood disease affecting humans and other animals caused by parasitic protozoans belonging to the Plasmodium type especially in developing countries. The gold standard method for the detection of malaria is through the microscopic method of chemically treated blood smears. We de...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700279
更新日期:2018-05-01 00:00:00
abstract::Photodynamic inactivation of bacteria (PDIB) is considered a new approach for the struggle against multiresistant bacteria. To achieve a sufficient level of bacteria killing, the photosensitizer must attach to and/or penetrate the bacteria and generate a sufficiently high amount of singlet oxygen. To optimize PDIB, th...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.200900106
更新日期:2010-06-01 00:00:00
abstract::In this work we present how to entirely remove the scattering ambiguity present in existing multiphoton multifocal systems. This is achieved through the development and implementation of single-element detection systems that incorporate high-speed photon-counting electronics. These systems can be used to image entire ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201100139
更新日期:2012-05-01 00:00:00
abstract::Tumor metastasis to lymph nodes is an important contributory factor for cancer-related deaths despite recent developments in cancer therapy. In this study, we demonstrate that tumor in the proper axillary lymph node (PALN) of the mouse can be treated by the application of external laser light to trigger the unloading ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600241
更新日期:2017-12-01 00:00:00
abstract::Noninvasive body shaping is becoming a growing demand. The aim of this study was to investigate the efficacy and safety of the combined treatments of 1064 nm Nd:YAG and 2940 nm Er:YAG in noninvasive lipolysis and skin tightening. Ten females were enrolled, and all women's side of the waist or the lower part of the abd...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900083
更新日期:2019-09-01 00:00:00
abstract::Laser tweezers Raman spectroscopy (LTRS) as a label-free and noninvasive technology has been proven to be an ideal tool for analysis of single living cells, which provides important fingerprint information without interference from surrounding environments. For the first time, LTRS system was successfully used to exam...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800332
更新日期:2019-03-01 00:00:00
abstract::A new optical scattering contrast-agent based on polymer-nanoparticle encapsulated silver nanoplates (PESNs) is presented. Silver nanoplates were chosen due to the flexibility of tuning their plasmon frequencies. The polymer coating preserves their physical and optical properties and confers other advantages such as c...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201500177
更新日期:2016-07-01 00:00:00
abstract::223 Radium (223 Ra) is widely used in nuclear medicine to treat patients with osseous metastatic prostate cancer. In clinical practice 223 Ra cannot be imaged directly; however, gamma photons produced by its short-lived daughter nuclides can be captured by conventional gamma cameras. In this work, we show that 223 Ra ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700209
更新日期:2018-04-01 00:00:00