Abstract:
:The use of conventional fluorescence microscopy for characterizing tissue pathological states is limited by overlapping spectra and the dependence on excitation power and fluorophore concentration. Fluorescence lifetime imaging microscopy (FLIM) can overcome these limitations due to its insensitivity to fluorophore concentration, excitation power and spectral similarity. This study investigates the diagnosis of early cervical cancer using FLIM and a neural network extreme learning machine classifier. A concurrently high sensitivity and specificity of 92.8% and 80.2%, respectively, were achieved. The results suggest that the proposed technique can be used to supplement the traditional histopathological examination of early cervical cancer.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Gu J,Fu CY,Ng BK,Gulam Razul Ss,Lim SKdoi
10.1002/jbio.201200202subject
Has Abstractpub_date
2014-07-01 00:00:00pages
483-91issue
7eissn
1864-063Xissn
1864-0648journal_volume
7pub_type
杂志文章abstract::Nacre is a complex biomaterial made of aragonite-tablet bricks and organic mortar that is considerably resilient against breakage. Nacre has been studied with a wide range of laboratory techniques, leading to understanding key fundamentals and informing the creation of bio-inspired materials. In this article, we prese...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800026
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abstract::The effect of cetyl-trimethylammonium bromide (CTAB) on enhancing the fluorescence resonance energy transfer (FRET) between two dye-conjugated DNA strands was studied using fluorescence emission spectroscopy and dynamic light scattering (DLS). For hybridized DNA where one strand is conjugated with a TAMRA donor and th...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201500221
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abstract::The class of PEGylated emulsifiers finds broad application in the pharmaceutical and cosmetic industry. We target on one of the categories of polyethylene glycol (PEG) alkyl ethers with different lipophilic and hydrophilic chain length and aim to examine their effects on the skin comprehensively. In this study, we emp...
journal_title:Journal of biophotonics
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abstract::One of the challenges in transcranial low-level laser therapy (LLLT) is to optimally choose illumination parameters, such as wavelength. However, there is sparse study on the wavelengths comparison especially on human transcranial LLLT. Here, we employed Monte Carlo modeling and visible human phantom to compute the pe...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800173
更新日期:2019-02-01 00:00:00
abstract::Whole slide imaging (WSI) has moved digital pathology closer to diagnostic practice in recent years. Due to the inherent tissue topography variability, accurate autofocusing remains a critical challenge for WSI and automated microscopy systems. The traditional focus map surveying method is limited in its ability to ac...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
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abstract::Photodynamic therapy (PDT) uses photosensitizers and visible light in combination with molecular oxygen to produce reactive oxygen species (ROS) that kill malignant cells by apoptosis and/or necrosis, shut down the tumor microvasculature and stimulate the host immune system. The excited singlet state of oxygen (1 O2 )...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.201600055
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abstract::The purpose of this study was to evaluate thermal and near-infrared (NIR) reflectance imaging methods for the assessment of the activity of root caries lesions. In addition, changes in the lesion structure were monitored with polarization sensitive optical coherence tomography (PS-OCT). Artificial bovine and natural r...
journal_title:Journal of biophotonics
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journal_title:Journal of biophotonics
pub_type: 杂志文章
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更新日期:2019-12-01 00:00:00
abstract::Lasers/LEDs demonstrate therapeutic effects for a range of biomedical applications. However, a consensus on effective light irradiation parameters and efficient and reliable measurement techniques remain limited. The objective here is to develop, characterise and demonstrate the application of LED arrays in order to p...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600273
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journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800018
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abstract::Malaria is a life-threatening infectious blood disease affecting humans and other animals caused by parasitic protozoans belonging to the Plasmodium type especially in developing countries. The gold standard method for the detection of malaria is through the microscopic method of chemically treated blood smears. We de...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700279
更新日期:2018-05-01 00:00:00
abstract::Currently, the targeted treatment of tumor based on the tumor microenvironment is newly developed. Blood vessels are the key parts in the tumor microenvironment, which is taken as a new visible target for tumor therapy. Multiphoton microscopy (MPM), based on the second harmonic generation and two-photon excited fluore...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900006
更新日期:2019-09-01 00:00:00
abstract::Articular cartilage posesses unique material properties due to a complex depth-dependent composition of sub-components. Raman spectroscopy has proven valuable in quantifying this composition through cartilage cross-sections. However, cross-sectioning requires tissue destruction and is not practical in situ. In this wo...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000289
更新日期:2021-01-01 00:00:00
abstract::Biophotonics is a highly interdisciplinary field where physicists, chemists, biologists, physicians and engineers work together to solve the problems appearing in biology and medicine. In China, the Biophotonics discipline is often referred to as Biomedical Photonics, under the first-level disciplines Biomedical Engin...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201790012
更新日期:2017-12-01 00:00:00
abstract::The authors have developed a tomographic diffractive microscope that combines microholography with illumination from an angular synthetic aperture. It images specimens relative to their complex index of refraction distribution (index and absorption) and permits imaging of unlabelled specimens, with high lateral resolu...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.200900094
更新日期:2010-07-01 00:00:00
abstract::This paper comments on the article "Retinal pulse wave velocity measurement using spectral-domain optical coherence tomography" by Qian Li et al. The authors propose a method to determine the pulse wave velocity in retinal arteries and veins. This method should enable a noninvasive determination of biomechanical prope...
journal_title:Journal of biophotonics
pub_type: 评论,杂志文章
doi:10.1002/jbio.201700347
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abstract::It is well known that, under exposure to bright light, eggs tend to hatch earlier than control, without any damage to the birds. This report aims to systematically show the effect and establishes a proposal for a possible application to accelerate chicken egg formation, which could be extrapolated or adapted as a grea...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700046
更新日期:2018-02-01 00:00:00
abstract::223 Radium (223 Ra) is widely used in nuclear medicine to treat patients with osseous metastatic prostate cancer. In clinical practice 223 Ra cannot be imaged directly; however, gamma photons produced by its short-lived daughter nuclides can be captured by conventional gamma cameras. In this work, we show that 223 Ra ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700209
更新日期:2018-04-01 00:00:00
abstract::Solar radiation exposure is recognised to be a significant contributor to the development of skin cancer. Monitoring the simultaneous and consecutive mechanisms of interaction could provide a greater understanding of the process of photocarcinogenesis. This work presents an analysis of the biochemical and morphologica...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000337
更新日期:2020-10-24 00:00:00
abstract::Near-infrared light allows measuring tissue oxygenation. These measurements relay on oxygenation-dependent absorption spectral changes. However, the tissue scattering, which is also spectral dependent, introduces an intrinsic error. Most methods focus on the volume reflectance from a semi-infinite sample. We have prop...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700208
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abstract::Single-molecule localisation based super-resolution fluorescence imaging produces maps of the coordinates of fluorescent molecules in a region of interest. Cluster analysis algorithms provide information concerning the clustering characteristics of these molecules, often through the generation of cluster heat maps bas...
journal_title:Journal of biophotonics
pub_type: 杂志文章
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journal_title:Journal of biophotonics
pub_type: 杂志文章
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abstract::Myelin is a key component of the peripheral nervous system, whose structure anomaly in the digital skin is implicated in neuropathy. Here we demonstrate an in vivo labeling and imaging technique, capable of visualizing myelin sheaths deep in the mouse digital skin. Through material characterization, we verify that 3-p...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000321
更新日期:2020-12-01 00:00:00
abstract::A simple scheme for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy is based on the spectral focusing of ultrafast-oscillator-derived pump/probe light and synchronous photonic crystal fiber (PCF) fiber-generated broadband Stokes light. To date, such schemes allowed rapid hyperspectral imaging throug...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200171
更新日期:2014-01-01 00:00:00
abstract::Diffuse reflectance spectroscopy is a rapidly growing technology in the biophotonics community where it has shown promise in its ability to classify different tissues. In the steady-state domain a wide spectrum of clinical applications is supported with this technology ranging from diagnostic to guided interventions. ...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.201300198
更新日期:2015-01-01 00:00:00
abstract::Lasers are used in dentistry for a variety of indications. One of these is the disinfection of root canals or the sterilization of residual caries. Many studies have demonstrated the capacity to kill bacteria for lasers but the fundamental mechanism of the laser effect remains quite unclear. With our experiments we wa...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201000007
更新日期:2010-06-01 00:00:00
abstract::Limb perfusion monitoring is critical for diabetes mellitus (DM) patients as they are vulnerable to vascular complications due to prolonged hyperglycemia. However, current clinical approaches are ineffective in vascular imaging and in assessing vascular function in lower limbs. In this work, a concave ultrasound trans...
journal_title:Journal of biophotonics
pub_type: 信件
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更新日期:2020-08-01 00:00:00
abstract::This study presents numerical simulations of UVC light propagation through seven different filtered face respirators (FFR) to determine their suitability for Ultraviolet germicidal inactivation (UVGI). UV propagation was modeled using the FullMonte program for two external light illuminations. The optical properties o...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000232
更新日期:2020-12-01 00:00:00
abstract::Localization microscopy methods like Stochastic Optical Reconstruction Microscopy (STORM) are very well suited for exploring clustering of proteins, as the data inherently provide a list of molecular coordinates. Here we use state-of-art cluster analysis algorithms (DBSCAN) to explore the clustering behaviour of diffe...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800080
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abstract::We have investigated the ability of dual-frequency Coherent Antistokes Raman Scattering (D-CARS) micro-spectroscopy, based on femtosecond pulses (100 fs or 5 fs) spectrally focussed by glass dispersion, to distinguish the chemical composition of micron-sized lipid droplets consisting of different triglycerides types (...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200197
更新日期:2014-01-01 00:00:00