Hyperspectral multimodal CARS microscopy in the fingerprint region.

Abstract:

:A simple scheme for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy is based on the spectral focusing of ultrafast-oscillator-derived pump/probe light and synchronous photonic crystal fiber (PCF) fiber-generated broadband Stokes light. To date, such schemes allowed rapid hyperspectral imaging throughout the CH/OH high frequency region (2700-4000 cm(-1) ). Here we extend this approach to the middle (1640-3300 cm(-1) ) and fingerprint regions (850-1800 cm(-1) ) of the Raman spectrum. Our simple integrated approach to rapid hyperspectral CARS microscopy in the fingerprint region is demonstrated by applications to label-free multimodal imaging of cellulose and bulk bone, including use of the phosphate resonance at 960 cm(-1) .

journal_name

J Biophotonics

journal_title

Journal of biophotonics

authors

Pegoraro AF,Slepkov AD,Ridsdale A,Moffatt DJ,Stolow A

doi

10.1002/jbio.201200171

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

49-58

issue

1-2

eissn

1864-063X

issn

1864-0648

journal_volume

7

pub_type

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