Abstract:
:A simple scheme for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy is based on the spectral focusing of ultrafast-oscillator-derived pump/probe light and synchronous photonic crystal fiber (PCF) fiber-generated broadband Stokes light. To date, such schemes allowed rapid hyperspectral imaging throughout the CH/OH high frequency region (2700-4000 cm(-1) ). Here we extend this approach to the middle (1640-3300 cm(-1) ) and fingerprint regions (850-1800 cm(-1) ) of the Raman spectrum. Our simple integrated approach to rapid hyperspectral CARS microscopy in the fingerprint region is demonstrated by applications to label-free multimodal imaging of cellulose and bulk bone, including use of the phosphate resonance at 960 cm(-1) .
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Pegoraro AF,Slepkov AD,Ridsdale A,Moffatt DJ,Stolow Adoi
10.1002/jbio.201200171subject
Has Abstractpub_date
2014-01-01 00:00:00pages
49-58issue
1-2eissn
1864-063Xissn
1864-0648journal_volume
7pub_type
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