Abstract:
:Whole-body bioimaging was used to study dissemination of vaccinia virus (VACV) in normal and in immune deficient (nu(-)/nu(-)) mice protected from lethality by postchallenge administration of ST-246. Total fluxes were recorded in the liver, spleen, lungs, and nasal cavities of live mice after intranasal infection with a recombinant IHD-J-Luc VACV expressing luciferase. Areas under the flux curve were calculated for individual mice to assess viral loads. Treatment for 2 to 5 days of normal BALB/c mice with ST-246 at 100 mg/kg starting 24 h postchallenge conferred 100% protection and reduced viral loads in four organs compared to control mice. Mice also survived after 5 days of treatment with ST-246 at 30 mg/kg, and yet the viral loads and poxes were higher in these mice compared to 100-mg/kg treatment group. Nude mice were not protected by ST-246 alone or by 10 million adoptively transferred T cells. In contrast, nude mice that received T cells and 7-day treatment with ST-246 survived infection and exhibited reduced viral loads compared to nonreconstituted and ST-246-treated mice after ST-246 was stopped. Similar protection of nude mice was achieved using adoptively transferred 1.0 and 0.1 million, but not 0.01 million, purified T cells or CD4(+) or CD8(+) T cells in conjunction with ST-246 treatment. These data suggest that ST-246 protects immunocompetent mice from lethality and reduces viral dissemination in internal organs and poxvirus lesions. Furthermore, immune-deficient animals with partial T cell reconstitution can control virus replication after a course of ST-246 and survive lethal vaccinia virus challenge.
journal_name
J Viroljournal_title
Journal of virologyauthors
Zaitseva M,Shotwell E,Scott J,Cruz S,King LR,Manischewitz J,Diaz CG,Jordan RA,Grosenbach DW,Golding Hdoi
10.1128/JVI.03426-12subject
Has Abstractpub_date
2013-05-01 00:00:00pages
5564-76issue
10eissn
0022-538Xissn
1098-5514pii
JVI.03426-12journal_volume
87pub_type
杂志文章abstract::Two human papillomavirus type 16 (HPV 16)-immortalized human keratinocyte cell lines (HPK) were shown to have retained the ability for differentiation after subcutaneous injection into nude mice. These properties were maintained even at late passage. HPK cells gave rise to transiently growing cysts which exhibited an ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.2.796-804.1991
更新日期:1991-02-01 00:00:00
abstract::Given the current difficulties generating vaccine-induced neutralizing antibodies to human immunodeficiency virus (HIV), the focus of the vaccine community has shifted toward creating cytotoxic-T-lymphocyte (CTL)-based vaccines. Recent reports of CTL-based vaccine trials in macaques challenged with simian/human immuno...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.24.13348-13360.2003
更新日期:2003-12-01 00:00:00
abstract::Rhesus monkey rhadinovirus (RRV) is a gammaherpesvirus that is closely related to human Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8). RRV is the closest relative to KSHV that has a fully sequenced genome and serves as an in vitro and an in vivo model system for KSHV. The latency-associated nuclear antigen (LAN...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00704-09
更新日期:2009-10-01 00:00:00
abstract::Phosphonoformate (foscarnet) is a pyrophosphate (PP(i)) analogue and a potent inhibitor of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT), acting through the PP(i) binding site on the enzyme. HIV-1 RT can unblock a chain-terminated DNA primer by phosphorolytic transfer of the terminal residue t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.11.6127-6137.2003
更新日期:2003-06-01 00:00:00
abstract::Adeno-associated virus (AAV) is a single-stranded DNA virus which requires adenovirus as a helper for productive infection. We studied whether intracellular AAV DNA in KB cells was present in a chromatin-like structure by digesting infected cell nuclei with micrococcal nuclease. Virus DNA was detected by agarose gel e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.1.79-87.1983
更新日期:1983-10-01 00:00:00
abstract::Infection of newborn Lewis rats with Borna disease virus (neonatal Borna disease [NBD]) results in cerebellar damage without the cellular inflammation associated with infections in later life. Purkinje cell (PC) damage has been reported for several models of early-life viral infection, including NBD; however, the time...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02245-06
更新日期:2007-03-01 00:00:00
abstract::The integrin alpha(v)beta3 has been shown to act as the receptor for internalization of foot-and-mouth disease virus (FMDV) (A12), with attachment being through a highly conserved RGD motif located on the G-H loop of viral capsid protein VP1. In addition, however, we have recently shown that efficient infection of cul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8357-8361.1997
更新日期:1997-11-01 00:00:00
abstract::Porcine circovirus 2 (PCV2) is a T=1 nonenveloped icosahedral virus that has had severe impact on the swine industry. Here we report the crystal structure of an N-terminally truncated PCV2 virus-like particle at 2.3-Å resolution, and the cryo-electron microscopy (cryo-EM) image reconstruction of a full-length PCV2 vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00737-11
更新日期:2011-08-01 00:00:00
abstract::T7+ phage are unable to plate on a strain of Shigella sonnei D2 371-48. Spontaneous phage mutants arise (ss--mutants) that are able to plate on this strain of Shigella. We have shown by complementation studies and genetic crosses that the ss--mutation maps in gene 10, the structural gene for the major protein of the c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.3.694-701.1977
更新日期:1977-06-01 00:00:00
abstract::Infectious pancreatic necrosis virus of fish, infectious bursal disease virus of chickens, Tellina virus and oyster virus of bivalve molluscs, and drosophila X virus of Drosophila melanogaster are naked icosahedral viruses with an electron microscopic diameter of 58 to 60 nm. The genome of each of these viruses consis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.2.593-605.1979
更新日期:1979-11-01 00:00:00
abstract::Nonglucosylated and fragmented T4 DNA shows a gene-specific variation in transformation efficiency. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.2.397-400.1973
更新日期:1973-08-01 00:00:00
abstract::The S1 gene nucleotide sequences of 10 type 3 (T3) reovirus strains were determined and compared with the T3 prototype Dearing strain in order to study sequence diversity in strains of a single reovirus serotype and to learn more about structure-function relationships of the two S1 translation products, sigma 1 and si...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.4842-4850.1990
更新日期:1990-10-01 00:00:00
abstract::Primary telencephalic cultures derived from neonatal Wistar Furth rats were able to support the growth of coronavirus JHM if a viable neuronal population was maintained. This occurred under serum-free defined, but not serum-supplemented, growth conditions. The importance of neurons in establishing infections in mixed ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.9.5013-5028.1991
更新日期:1991-09-01 00:00:00
abstract::The chimeric recombinant virus rHPIV3-N(B), a version of human parainfluenza virus type 3 (HPIV3) that is attenuated due to the presence of the bovine PIV3 nucleocapsid (N) protein open reading frame (ORF) in place of the HPIV3 ORF, was modified to encode the measles virus hemagglutinin (HA) inserted as an additional,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.21.10498-10504.2001
更新日期:2001-11-01 00:00:00
abstract::Poliovirus (PV) is easily transferred to humans orally; however, no rodent model for oral infections has been developed because of the alimentary tract's low sensitivity to the virus. Here we showed that PV is inactivated by the low pH of the gastric contents in mice. The addition of 3% NaHCO3 to the viral inoculum in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02675-06
更新日期:2007-08-01 00:00:00
abstract::An effective vaccine for AIDS may require development of novel vectors capable of eliciting long-lasting immune responses. Here we report the development and use of replication-competent and replication-defective strains of recombinant herpes simplex virus (HSV) that express envelope and Nef antigens of simian immunod...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.17.7745-7754.2000
更新日期:2000-09-01 00:00:00
abstract::Multispecific CD8(+) T-cell responses are thought to be important for the control of acute hepatitis C virus (HCV) infection, but to date little information is actually available on the breadth of responses at early time points. Additionally, the influence of early therapy on these responses and their relationships to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.12979-12988.2005
更新日期:2005-10-01 00:00:00
abstract::Cytotoxic T lymphocytes (CTL) play an important role in recovery from a number of viral infections. They are also implicated in virus-induced immunopathology as best demonstrated in lymphocytic choriomeningitis virus (LCMV) infection of adult immunocompetent mice. In the present study, the structure of the T-cell rece...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.5919-5926.1990
更新日期:1990-12-01 00:00:00
abstract::The Epstein-Barr virus (EBV) genome contains an open reading frame, BHRF1, that encodes a presumptive membrane protein with sequence similarity to the proto-oncogene bcl2, which is linked to human B-cell follicular lymphoma. Potential roles for BHRF1 in EBV's ability to growth transform human B cells and to replicate ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.1899-1906.1992
更新日期:1992-04-01 00:00:00
abstract::The major adenovirus (Ad) capsid proteins hexon, penton, and fiber influence the efficiency and tropism of gene transduction by Ad vectors. Fiber is the high-affinity receptor binding protein that serves to mediate cell attachment in vitro when using coxsackie-adenovirus receptor (CAR)-containing cell lines. This cont...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.18.11627-11637.2005
更新日期:2005-09-01 00:00:00
abstract::Coronavirus subgenomic mRNA possesses a 5'-end leader sequence which is derived from the 5' end of genomic RNA and is linked to the mRNA body sequence. This study examined whether coronavirus transcription involves a discontinuous transcription step; the possibility that a leader sequence from mouse hepatitis virus (M...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2615-2623.1994
更新日期:1994-04-01 00:00:00
abstract::Expression of the two bovine papillomavirus type 1 (BPV-1) late genes, L1 and L2, coding for the two capsid proteins, is limited to terminally differentiated keratinocytes in bovine fibropapillomas. This pattern of expression is determined both by the activity of the late promoter and by the inhibition of late region ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.5806-5812.1991
更新日期:1991-11-01 00:00:00
abstract::Transient transfection assays indicated that A2L is one of three vaccinia virus intermediate genes that are required for the transcriptional transactivation of viral late genes. We have expressed the A2L open reading frame in Escherichia coli and shown by blotting experiments that the 26-kDa protein binds zinc, a prop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.10.5749-5753.1993
更新日期:1993-10-01 00:00:00
abstract::We sequenced the genes coding for the two neutralization proteins, VP4 and VP7, of human rotavirus strains L26 and L27 with subgroup I specificity but the long RNA pattern. The deduced VP7 amino acid sequence of strains L26 and L27 showed a low homology (73.6 to 81.9%) to those of rotavirus strains of the established ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5640-5644.1990
更新日期:1990-11-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is the infectious cause of Kaposi's sarcoma, primary effusion lymphoma, and plasmablastic multicentric Castleman's disease. STAT3 has been shown to be important for the maintenance of primary effusion lymphoma cells in culture and is chronically activated in many tumor ce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01769-06
更新日期:2007-03-01 00:00:00
abstract::During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partia...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00696-10
更新日期:2010-08-01 00:00:00
abstract::Early stages of infection by the mouse polyomavirus have been studied using HeLa cells stably expressing small interfering RNA to protein disulfide isomerase (PDI). Infectibility measured by nuclear T antigen expression was reduced commensurately with the degree of PDI downregulation. Infectibility was restored by tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01117-06
更新日期:2006-11-01 00:00:00
abstract::Initial studies determined whether intraperitoneal (i.p.) injection of BALB/c mice with 0.1, 1.0, and 10 mg of adriamycin (a cardiotoxic anthracycline antibiotic) for times ranging between 1 and 9 weeks prior to i.p. injection of 10(5) PFU of coxsackievirus B3 (CVB3) would alter the severity of virus-induced myocardit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6541-6546.1992
更新日期:1992-11-01 00:00:00
abstract::An in vitro assay was developed to investigate endonuclease activity of Thogoto virus, a tick-borne orthomyxovirus. Endonuclease activity relied on an interaction between the 3' and 5' termini of virion RNA (vRNA) and not those of cRNA. Evidence was obtained that cap structures are cleaved directly from cap donors and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2305-2309.1998
更新日期:1998-03-01 00:00:00
abstract::Productive viral infection often depends on the manipulation of the cytoskeleton. Herpesviruses, including rhesus monkey rhadinovirus (RRV) and its close homolog, the oncogenic human gammaherpesvirus Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 (KSHV/HHV8), exploit microtubule (MT)-based retrograde tran...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00304-17
更新日期:2017-08-10 00:00:00