Abstract:
:Porcine circovirus 2 (PCV2) is a T=1 nonenveloped icosahedral virus that has had severe impact on the swine industry. Here we report the crystal structure of an N-terminally truncated PCV2 virus-like particle at 2.3-Å resolution, and the cryo-electron microscopy (cryo-EM) image reconstruction of a full-length PCV2 virus-like particle at 9.6-Å resolution. This is the first atomic structure of a circovirus. The crystal structure revealed that the capsid protein fold is a canonical viral jelly roll. The loops connecting the strands of the jelly roll define the limited features of the surface. Sulfate ions interacting with the surface and electrostatic potential calculations strongly suggest a heparan sulfate binding site that allows PCV2 to gain entry into the cell. The crystal structure also allowed previously determined epitopes of the capsid to be visualized. The cryo-EM image reconstruction showed that the location of the N terminus, absent in the crystal structure, is inside the capsid. As the N terminus was previously shown to be antigenic, it may externalize through viral "breathing."
journal_name
J Viroljournal_title
Journal of virologyauthors
Khayat R,Brunn N,Speir JA,Hardham JM,Ankenbauer RG,Schneemann A,Johnson JEdoi
10.1128/JVI.00737-11subject
Has Abstractpub_date
2011-08-01 00:00:00pages
7856-62issue
15eissn
0022-538Xissn
1098-5514pii
JVI.00737-11journal_volume
85pub_type
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