Abstract:
:An in vitro assay was developed to investigate endonuclease activity of Thogoto virus, a tick-borne orthomyxovirus. Endonuclease activity relied on an interaction between the 3' and 5' termini of virion RNA (vRNA) and not those of cRNA. Evidence was obtained that cap structures are cleaved directly from cap donors and that cleavage does not occur after pyrimidines. A 5' hook structure, present in the vRNA promoter but not the cRNA promoter, was introduced into cRNA promoter mutants. These mutants stimulated endonuclease activity, although at levels slightly lower than that of vRNA. The ability of the cRNA promoter to stimulate endonuclease activity when mutated to contain a 5' hook structure indicates that this structure constitutes a switching mechanism for endonuclease activity between the vRNA and cRNA promoters.
journal_name
J Viroljournal_title
Journal of virologyauthors
Leahy MB,Dessens JT,Pritlove DC,Nuttall PAdoi
10.1128/JVI.72.3.2305-2309.1998subject
Has Abstractpub_date
1998-03-01 00:00:00pages
2305-9issue
3eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
杂志文章abstract::Rotavirus is the leading global cause of diarrheal mortality for unvaccinated children under 5 years of age. The outer capsid of rotavirus virions consists of VP7 and VP4 proteins, which determine viral G and P types, respectively, and are primary targets of neutralizing antibodies. Successful vaccination depends upon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01476-18
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abstract::An unusual set of 3' coterminal, spliced mRNAs transcribed through the BamHI A fragment have been previously identified in nasopharyngeal carcinoma (NPC) tissues. These RNAs have also been detected at low levels in Burkitt's lymphoma (BL) cell lines and Epstein-Barr virus (EBV)-transformed lymphocytes. Sequence analys...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.2765-2771.1997
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doi:10.1128/JVI.00046-06
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journal_title:Journal of virology
pub_type: 杂志文章
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abstract::The complete genomic sequence of a new Muscovy duck-origin reovirus (N-MDRV), strain J18 from China, was determined. The virus has a tricistronic S1 genome segment that is distinct from the originally described MDRV, which possesses a bicistronic S4 genome segment. Pairwise comparisons and phylogenetic analyses sugges...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02230-12
更新日期:2012-11-01 00:00:00
abstract::Recently, recombinant baculoviruses have been used to show that expression of six herpes simplex virus type 1 genes results in the formation of capsid-like particles. We have applied cryoelectron microscopy and three-dimensional image reconstruction to establish their structural authenticity to a resolution of approxi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7362-7366.1995
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abstract::Simian virus 40 large T antigen (large T) in the early and the late phases of infection differs significantly in its sequence-specific DNA-binding and ATPase activities, indicating that different large-T populations participate in virus-specific events at various stages of the infectious cycle. To further characterize...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2308-2316.1989
更新日期:1989-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8923-8927.1997
更新日期:1997-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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abstract::Human cytomegalovirus (HCMV) protein pUL38 has been shown to prevent premature cell death by antagonizing cellular stress responses; however, the underlying mechanism remains unknown. In this study, we identified the host protein ubiquitin-specific protease 24 (USP24) as an interaction partner of pUL38. Mutagenesis an...
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abstract::Herpes simplex virus type 1 (HSV-1) is a DNA virus that acquires an envelope by budding into the inner nuclear membrane of an infected cell. Recombinant HSV-1 lacking the U(L)34 gene cannot undergo this event. U(L)34 and U(L)31, another viral protein, colocalize in an infected cell and are necessary and sufficient to ...
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pub_type: 杂志文章
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abstract::The baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) initiates apoptosis in diverse insects through events triggered by virus DNA (vDNA) replication. To define the proapoptotic pathway and its role in antivirus defense, we investigated the link between the host's DNA damage response (DDR) a...
journal_title:Journal of virology
pub_type: 杂志文章
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abstract::The B19 parvovirus, a small single-stranded DNA virus of 5.4 kilobases, is pathogenic in humans. B19 has remarkable specificity for erythroid progenitor cells and has been propagated in vitro only with human erythroid bone marrow. Replication of viral DNA and the viral protein products of B19 appear similar to those o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2395-2406.1987
更新日期:1987-08-01 00:00:00
abstract::Epstein-Barr virus (EBV) nuclear antigen 3C (EBNA-3C) is one of five viral nuclear proteins that are essential for EBV-induced immortalization of primary human B lymphocytes in vitro. Previous studies have implied that EBNA-3C acts as a transcription factor. Using transient transfection assays, we demonstrate that EBN...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3624-3630.1995
更新日期:1995-06-01 00:00:00
abstract::The transforming proteins of the small DNA tumor viruses, simian virus 40 (SV40), adenovirus, and human papillomavirus (HPV) target a number of identical cellular regulators whose functional abrogation is required for transformation. However, while both adenovirus E1A and SV40 large T transforming properties also depe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6209-6219.1999
更新日期:1999-08-01 00:00:00
abstract::Respiratory syncytial virus (RSV) produces intense pulmonary inflammation, in part through its ability to induce chemokine synthesis in infected airway epithelial cells. RANTES (regulated upon activation, normally T-cell expressed and presumably secreted) is a CC chemokine which recruits and activates monocytes, lymph...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.14.6428-6439.2001
更新日期:2001-07-01 00:00:00
abstract::Purified rabies virions, unlabeled or labeled with radioactive amino acids or d-glucosamine, were dissociated into their polypeptides by treatment with sodium dodecyl sulfate in a reducing environment and fractionated by electroiphoresis in sodium dodecyl sulfate-containing polyacrylamide gel. The molecular weights of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.2.241-249.1971
更新日期:1971-02-01 00:00:00
abstract::We have constructed new murine leukemia virus (MLV)-based vectors (TIN vectors) which, following integration, contain human immunodeficiency virus (HIV) type 1 U3 and R sequences in place of the MLV U3 and R regions. This provides, for the first time, single transcriptional unit retroviral vectors under the control of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.8234-8240.1996
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abstract::All presently available replication-competent proviral clones of human immunodeficiency virus type 1 (HIV-1) are derived from cell culture-amplified virus. Since tissue culture is highly selective for viral strains with an in vitro growth advantage, such clones may not be representative of the biologically relevant vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.3973-3985.1991
更新日期:1991-08-01 00:00:00
abstract::Open reading frame 45 (ORF45) of Kaposi's sarcoma-associated herpesvirus 8 (KSHV) is an immediate-early phosphorylated tegument protein and has been shown to play important roles at both early and late stages of viral infection. Homologues of ORF45 exist only in gammaherpesviruses, and their homology is limited. These...
journal_title:Journal of virology
pub_type: 杂志文章
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abstract::We investigated how the broadly neutralizing monoclonal antibody 2F5 affects the human immunodeficiency virus type 1 envelope glycoprotein as it undergoes receptor-induced conformational changes and show that 2F5 binds both native and fusion-intermediate conformations, suggesting inhibition of a late step in virus ent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2627-2631.2004
更新日期:2004-03-01 00:00:00
abstract::We have used an antisense RNA approach in the analysis of gene function in human cytomegalovirus (HCMV). An astrocytoma cell line (U373-MG) that is permissive for virus replication was permanently transfected with a construct bearing sequence from HCMV UL44 (coding for the major late DNA-binding protein, ppUL44, also ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2047-2057.1995
更新日期:1995-04-01 00:00:00
abstract::The protein product of the v-myb oncogene of avian myeloblastosis virus, p48v-myb, differs structurally in several ways from its normal cellular homolog, p75c-myb. We demonstrated that the 11 specific amino acid substitutions found in two independent molecular clones of this virus were not required for the transformat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.1093-1096.1988
更新日期:1988-03-01 00:00:00
abstract::Interspecies transmission of the transmissible spongiform encephalopathies (TSEs), or prion diseases, can result in the adaptation and selection of TSE strains with an expanded host range and increased virulence such as in the case of bovine spongiform encephalopathy and variant Creutzfeldt-Jakob disease. To investiga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.12.5542-5547.2000
更新日期:2000-06-01 00:00:00
abstract::SF162 is a primary (PR), non-syncytium-inducing, macrophagetropic human immunodeficiency virus type 1 (HIV-1) clade B isolate which is resistant to antibody-mediated neutralization. Deletion of the first or second hypervariable envelope gp120 region (V1 or V2 loop, respectively) of this virus does not abrogate its abi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.7840-7845.1998
更新日期:1998-10-01 00:00:00
abstract::Small-t antigen of simian virus 40 renders CV-1 cells resistant to growth arrest induced by theophylline and other methylxanthines. Elevated levels of cyclic AMP are not involved in growth arrest of CV-1 cells by methylxanthines, and small-t antigen does not alter cyclic AMP levels dramatically after infection. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.3.876-878.1985
更新日期:1985-06-01 00:00:00
abstract::The DNA sequence of 3,240 nucleotides of the XbaI G fragment located in the unique long (UL) region of the equine herpesvirus 1 genome revealed two major open reading frames (ORFs) designated UL3 and UL4. The UL3 ORF of 470 amino acids (aa) maps at nucleotides (nt) 4450 to 3038 from the long terminus, and its predicte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5363-5372.1992
更新日期:1992-09-01 00:00:00
abstract::The sequence of the human immunodeficiency virus type 2 circle junction was determined. The most common sequence found between the conserved CA and TG dinucleotides at the ends of the integrated provirus was five bases long (GGTAC). This suggests that the integration of human immunodeficiency virus type 2 DNA is accom...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3906-3910.1991
更新日期:1991-07-01 00:00:00
abstract::The XC rat cell line was found to support the replication of a strain of the Moloney murine sarcoma-leukemia virus. In growth curve experiments cytopathology was paralleled by the production of murine sarcoma virus and leukemia virus progeny having the biologic, antigenic, and biophysical properties of the infecting v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.1.146-149.1973
更新日期:1973-01-01 00:00:00
abstract::In most prion diseases, infectivity accumulates in lymphoreticular organs early after infection. Defects in hematopoietic compartments, such as impaired B-cell maturation, or in stromal compartments, such as abrogation of follicular dendritic cells, can delay or prevent lymphoreticular prion colonization. However, the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.15.7097-7106.2001
更新日期:2001-08-01 00:00:00