Abstract:
:Epstein-Barr virus (EBV) nuclear antigen 3C (EBNA-3C) is one of five viral nuclear proteins that are essential for EBV-induced immortalization of primary human B lymphocytes in vitro. Previous studies have implied that EBNA-3C acts as a transcription factor. Using transient transfection assays, we demonstrate that EBNA-3C has two effects on reporter genes that are linked to the latent membrane protein 1 promoter, (i) low-level activation by EBNA-3C alone, as well as potentiation of EBNA-2-mediated transactivation, and (ii) inhibition of the normally strong activation mediated by EBNA-2. These two disparate effects seem to be mediated at different stages following cell feeding. The inhibitory effect of EBNA-3C was localized to a known EBNA-2 response element that had previously been shown to be recognized by the DNA-binding protein RBP-J kappa. In addition, direct interaction between RBP-J kappa and EBNA-3C was observed by coimmunoprecipitation. Activation by EBNA-3C, however, seems to be achieved via sequences that are distinct from RBP-J kappa sites, since activation remained even after these sites had been mutated. Consistent with its ability to activate transcription, a region of EBNA-3C which has homology to the glutamine-rich activation domain of Sp1 can function as a transcription activation domain when it is fused to the heterologous DNA-binding domain of Gal4 and can partially restore the activity of a mutant EBNA-2 protein with a deletion in the transactivation domain. Collectively, these data strongly support the role of EBNA-3C as a transcriptional regulator.
journal_name
J Viroljournal_title
Journal of virologyauthors
Marshall D,Sample Cdoi
10.1128/JVI.69.6.3624-3630.1995subject
Has Abstractpub_date
1995-06-01 00:00:00pages
3624-30issue
6eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::Recent studies suggest the existence of cancer stem cells (CSC) and cancer progenitor cells (CPC), although strict definitions of neither CSC nor CPC have been developed. We have produced evidence that the principal oncoprotein of Epstein-Barr virus (EBV), latent membrane protein 1 (LMP1), which is associated with hum...
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pub_type: 杂志文章
doi:10.1128/JVI.00188-11
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pub_type: 杂志文章
doi:10.1128/JVI.01013-09
更新日期:2010-01-01 00:00:00
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doi:10.1128/JVI.63.7.3209-3212.1989
更新日期:1989-07-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.00315-12
更新日期:2012-05-01 00:00:00
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doi:10.1128/jvi.74.23.11055-11066.2000
更新日期:2000-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.4.507-512.1970
更新日期:1970-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.00777-09
更新日期:2009-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7187-7195.1995
更新日期:1995-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.01439-08
更新日期:2009-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01420-08
更新日期:2009-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.1.212-215.1986
更新日期:1986-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.3512-3526.1994
更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.76.23.11785-11792.2002
更新日期:2002-12-01 00:00:00
abstract::Processes by which human herpesviruses penetrate and are released from polarized epithelial cells, which have distinct apical and basolateral membrane domains differing in protein and lipid content, are poorly understood. We recently reported that human cytomegalovirus (CMV) mutants with deletions of the gene US9 form...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5717-5727.1998
更新日期:1998-07-01 00:00:00
abstract:UNLABELLED:Increased detection of enterovirus 68 (EV68) among patients with acute respiratory infections has been reported from different parts of the world in the late 2000s since its first detection in pediatric patients with lower-respiratory-tract infections in 1962. However, the underlying molecular mechanisms for...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03070-13
更新日期:2014-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02911-15
更新日期:2016-04-14 00:00:00
abstract::The RNA polymerase in the nucleocapsid of Pseudomonas phaseolicola bacteriophage phi 6 transcribed large, medium, and small single-stranded RNA from the viral double-stranded RNA genome by a semiconservative (displacement) mechanism. Approximately 23%, 63%, and 65% of the nucleocapsid particles in the assay mixture sy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.2.769-773.1980
更新日期:1980-02-01 00:00:00
abstract::Human CD317 (BST-2/tetherin) is an intrinsic immunity factor that blocks the release of retroviruses, filoviruses, herpesviruses, and arenaviruses. It is unclear whether CD317 expressed endogenously in rodent cells has the capacity to interfere with the replication of the retroviral rodent pathogen murine leukemia vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01067-10
更新日期:2010-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7369-7378.2004
更新日期:2004-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.8.4830-4841.1995
更新日期:1995-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00724-19
更新日期:2019-06-28 00:00:00
abstract::Human cytomegalovirus (HCMV) is the most common congenitally transmitted pathogen worldwide, impacting an estimated 1 million newborns annually. Congenital HCMV (cCMV) infection is a major global contributor to long-term neurologic deficits, including deafness, microcephaly, and neurodevelopmental delay, as well as to...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00030-18
更新日期:2018-03-14 00:00:00
abstract::Viral replication depends on specific interactions with host factors. For example, poliovirus RNA replication requires association with intracellular membranes. Brefeldin A (BFA), which induces a major rearrangement of the cellular secretory apparatus, is a potent inhibitor of poliovirus RNA replication. Most aspects ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.7.3378-3386.2004
更新日期:2004-04-01 00:00:00
abstract::2G12 is a broadly neutralizing anti-HIV-1 monoclonal human IgG1 antibody reactive with a high-mannose glycan cluster on the surface of glycoprotein gp120. A key feature of this very highly mutated antibody is domain exchange of the heavy-chain variable region (V(H)) with the V(H) of the adjacent Fab of the same immuno...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01111-10
更新日期:2010-10-01 00:00:00
abstract::Accurate and sensitive quantitation of infectious human immunodeficiency virus (HIV) has been difficult to achieve. In this report, a quantitative focal immunoassay (FIA) for HIV was developed using human HeLa cells rendered susceptible to HIV infection by introduction of the CD4 gene via a retrovirus vector. Infected...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.10.3779-3788.1988
更新日期:1988-10-01 00:00:00
abstract::The question of whether any mammalian cells are able to mount an effective RNA interference-mediated antiviral innate immune response has remained highly controversial. In this Gem, I review recent data addressing this important issue and propose a testable hypothesis that can explain many of the apparently contradict...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.01179-14
更新日期:2014-11-01 00:00:00
abstract::Major histocompatibility complex E (MHC-E) is a highly conserved nonclassical MHC-Ib molecule that tightly binds peptides derived from leader sequences of classical MHC-Ia molecules for presentation to natural killer cells. However, MHC-E also binds diverse foreign and neoplastic self-peptide antigens for presentation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00592-19
更新日期:2019-09-12 00:00:00
abstract::We describe spontaneous second-site reversions within the coat protein open reading frame that rescue the systemic-spread phenotype and increase virion stability of a mutant of brome mosaic virus. Based on the crystal structure of the related cowpea chlorotic mottle virus, we show that the modified residues are spatia...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.2500-2504.1997
更新日期:1997-03-01 00:00:00
abstract::Influenza virus infection initiates transcription of a variety of genes for cytokines such as tumor necrosis factor alpha (TNF-alpha), TNF-beta, interleukin 1 alpha, (IL-1 alpha), IL-1 beta, IL-2, IL-4, IL-6, IL-10, granulocyte macrophage colony-stimulating factor, and gamma interferon. However, the mechanism by which...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1480-1484.1995
更新日期:1995-03-01 00:00:00
abstract::Certain murine leukemia viruses (MLVs) can induce progressive noninflammatory spongiform neurodegeneration similar to that caused by prions. The primary MLV determinants responsible have been mapped to within the env gene; however, it has remained unclear how env mediates disease, whether non-Env viral components are ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02210-10
更新日期:2011-03-01 00:00:00