Abstract:
:Accurate and sensitive quantitation of infectious human immunodeficiency virus (HIV) has been difficult to achieve. In this report, a quantitative focal immunoassay (FIA) for HIV was developed using human HeLa cells rendered susceptible to HIV infection by introduction of the CD4 gene via a retrovirus vector. Infected cells were identified by using human anti-HIV antibodies or mouse monoclonal antibodies specific for HIV together with secondary fluorescein- or peroxidase-conjugated antibody specific for mouse or human immunoglobulins. The assay identified cells infected with either wild-type or culture-adapted HIV isolates and was capable of detecting 1 positive cell in 10(6) cells. The FIA was also effective at detecting cell-free HIV, and in contrast to assays using A3.01, CEM, and other human leukemia cells, the FIA detected most wild-type HIV isolates. HIV neutralization could be determined by using the FIA, and two monoclonal antibodies reactive with HIV gp120 were found to neutralize only the LAV-IIIB strain of HIV. These monoclonal antibodies, as well as antibodies in serum samples from patients with acquired immune deficiency syndrome, were able to inhibit the spread of HIV infection in human lymphocyte suspension cultures but not in CD4-positive HeLa cells growing attached to plastic dishes.
journal_name
J Viroljournal_title
Journal of virologyauthors
Chesebro B,Wehrly Kdoi
10.1128/JVI.62.10.3779-3788.1988subject
Has Abstractpub_date
1988-10-01 00:00:00pages
3779-88issue
10eissn
0022-538Xissn
1098-5514journal_volume
62pub_type
杂志文章abstract::Gene transfer to differentiated airway epithelia with existing viral vectors is very inefficient when they are applied to the apical surface. This largely reflects the polarized distribution of receptors on the basolateral surface. To identify new receptor-ligand interactions that might be used to redirect vectors to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.9234-9239.2000
更新日期:2000-10-01 00:00:00
abstract::Adeno-associated virus type 2 (AAV-2) gene expression is tightly controlled by functions of the helper virus as well as by the products of its own viral rep gene. Double-immunofluorescence studies of Rep and VP protein expression in cells coinfected with AAV-2 and adenovirus type 2 showed that a large proportion of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8437-8447.1997
更新日期:1997-11-01 00:00:00
abstract::Mutations critical for the central nervous system (CNS) attenuation of the Sabin vaccine strains of poliovirus (PV) are located within the viral internal ribosome entry site (IRES). We examined the interaction of the IRESs of PV type 3 (PV3) and Sabin type 3 (Sabin3) with polypyrimidine tract-binding protein (PTB) and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.20.11097-11107.2004
更新日期:2004-10-01 00:00:00
abstract:UNLABELLED:Herpes simplex virus (HSV) replicates in the skin and mucous membranes, and initiates lytic or latent infections in sensory neurons. Assembly of progeny virions depends on the essential large tegument protein pUL36 of 3,164 amino acid residues that links the capsids to the tegument proteins pUL37 and VP16. O...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03167-15
更新日期:2016-05-12 00:00:00
abstract::Previous reports (H. Delius and J. B. Clements, J. Gen. Virol. 33:125-134, 1976; G. S. Hayward, R. J. Jacob, S. C. Wadsworth, and B. Roizman, Proc. Natl. Acad. Sci. U.S.A. 72:4243-4247, 1975; B. Roizman, G. S. Hayward, R. Jacob, S. W. Wadsworth, and R. W. Honess, Excerpta Med. Int. Congr. Ser. 2:188-198, 1974) have sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.2.448-457.1979
更新日期:1979-02-01 00:00:00
abstract::Mouse neuroblastoma cells containing intracisternal type A particles were treated with iododeoxyuridine and dexamethasone to induce the release of type C oncornavirus particles. For 5 days after treatment, antigenic markers and DNA polymerase activities specific to particles of each of the two types were assayed in th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.2.709-716.1976
更新日期:1976-08-01 00:00:00
abstract::Due to increasing concerns about human infection by various H7 influenza viruses, including recent H7N9 viruses, we evaluated the genetic relationships and cross-protective efficacies of three different Eurasian H7 avian influenza viruses. Phylogenic and molecular analyses revealed that recent Eurasian H7 viruses can ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02259-16
更新日期:2017-05-12 00:00:00
abstract::The amphotropic murine leukemia virus (MuLV) can infect cells from a number of mammals, including humans, via its specific receptor. Basic knowledge of amphotropic MuLV receptor expression is likely to be useful in the development and improvement of gene therapy protocols based on amphotropic-pseudotyped vectors. To i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4564-4570.1997
更新日期:1997-06-01 00:00:00
abstract::The current studies demonstrate complex and seemingly contradictory effects by gamma interferon (IFN-gamma) on Friend virus (FV) infection. Both temporal and tissue-specific effects were observed. During the first week of infection, IFN-gamma-deficiency caused increased levels of FV infection in multiple tissues. Surp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.5.2225-2232.2002
更新日期:2002-03-01 00:00:00
abstract:UNLABELLED:The antiviral role of TRIM E3 ligases in vivo is not fully understood. To test the hypothesis that TRIM5α and TRIM22 have differential transcriptional regulation and distinct anti-HIV roles according to infection phase and compartment, we measured TRIM5α, TRIM22, and type I interferon (IFN-I)-inducible myxov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03603-13
更新日期:2014-04-01 00:00:00
abstract::From the results of transient plasmid-based replication assays, it has been postulated that homologous regions (hrs) of Autographa californica nuclear polyhedrosis virus (AcMNPV) function as origins of viral DNA replication. However, these assays vary in specificity according to the methodology used and may not be dep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6967-6972.1996
更新日期:1996-10-01 00:00:00
abstract::Zika virus (ZIKV), a mosquito-transmitted flavivirus, is linked to microcephaly and other neurological defects in neonates and Guillain-Barré syndrome in adults. The molecular mechanisms regulating ZIKV infection and pathogenic outcomes are incompletely understood. Signaling by the mechanistic (mammalian) target of ra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01575-20
更新日期:2020-10-27 00:00:00
abstract::Heat shock protein 70 (Hsp70) is incorporated within the membrane of primate lentiviral virions. Here we demonstrate that Hsp70 is also incorporated into oncoretroviral virions and that it remains associated with membrane-stripped human immunodeficiency virus type 1 (HIV-1) virion cores. To determine if Hsp70 promotes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5557-5567.2005
更新日期:2005-05-01 00:00:00
abstract::The protein (VPg) linked to the 5' termini of poliovirus RNAs resolved into two species when subjected to non-equilibrium electrofocusing. The differently charged forms of VPg were not due to protein phosphorylation nor to variability of the number of phosphate residues associated with the nucleotide moiety remaining ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.3.863-871.1981
更新日期:1981-06-01 00:00:00
abstract::In addition to encoding the structural and regulatory proteins, many viruses encode auxiliary proteins, some of which have been shown to play important roles in lytic and latent states of the viruses. The human neurotropic JC virus (JCV) genome encodes an auxiliary protein called Agno whose function remains unknown. H...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.3.1476-1486.2001
更新日期:2001-02-01 00:00:00
abstract::Swainsonine, an inhibitor of glycoprotein processing, inhibits the formation of the normal oligosaccharide chain of the G protein of vesicular stomatitis virus. Thus, when vesicular stomatitis virus was grown in baby hamster kidney cells in the presence of swainsonine (15 to 500 ng/ml) and labeled with [2-(3)H]mannose...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.1.60-69.1983
更新日期:1983-04-01 00:00:00
abstract::ICP4 is a herpesvirus-encoded protein that is expressed during the immediate-early phase of productive infection and is required for efficient transcription of viral genes during the early and late phases of infection. Previous studies have shown that ICP4 is a component of specific protein-DNA complexes but have not ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.2.696-704.1989
更新日期:1989-02-01 00:00:00
abstract::Recently we reported that in vitro RNA transcripts complementary to the genome of hepatitis delta virus (HDV) contain a unique site at which self-cleavage can occur. Subsequent studies showed that a similar self-cleavage site was present on in vitro RNA transcripts of genomic HDV RNA. The same self-cleavage reactions ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.12.4439-4444.1988
更新日期:1988-12-01 00:00:00
abstract::Proteolytic processing of the dengue virus polyprotein is mediated by host cell proteases and the virus-encoded NS2B-NS3 two-component protease. The NS3 protease represents an attractive target for the development of antiviral inhibitors. The three-dimensional structure of the NS3 protease domain has been determined, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.24.13708-13716.2004
更新日期:2004-12-01 00:00:00
abstract:UNLABELLED:Ebola virus (EBOV) belongs to the group of nonsegmented negative-sense RNA viruses. The seven EBOV genes are separated by variable gene borders, including short (4- or 5-nucleotide) intergenic regions (IRs), a single long (144-nucleotide) IR, and gene overlaps, where the neighboring gene end and start signal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01863-14
更新日期:2014-11-01 00:00:00
abstract::Epstein-Barr virus (EBV) undergoes latent and lytic replication cycles, and its reactivation from latency to lytic replication is initiated by expression of the two viral immediate-early transactivators, Zta and Rta. In vitro, reactivation of EBV can be induced by anti-immunoglobulin, tetradecanoyl phorbol acetate, an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01602-10
更新日期:2011-03-01 00:00:00
abstract::Hepatitis E virus replicons containing the neomycin resistance gene expressed from open reading frames (ORFs) 2 and 3 were transfected into Huh-7 cells, and stable cell lines containing functional replicons were selected by constant exposure to G418 sulfate. Northern blot analyses detected full-length replicon RNA and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00046-06
更新日期:2006-06-01 00:00:00
abstract::Sequences present at the genomic termini of herpesviruses become linked during lytic-phase replication and provide the substrate for cleavage and packaging of unit length viral genomes. We have previously shown that homologs of the consensus herpesvirus cleavage-packaging signals, pac1 and pac2, are located at the lef...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.320-329.1998
更新日期:1998-01-01 00:00:00
abstract::Understanding the microbiome of ticks in Australia is of considerable interest given the ongoing debate over whether Lyme disease and its causative agent, the bacterium Borrelia burgdorferisensu lato, are present in Australia. The diversity of bacteria infecting Australian ticks has been studied using both culture- an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01358-18
更新日期:2019-01-17 00:00:00
abstract::The bacteriophage vB_YecM-ϕR1-37 (ϕR1-37) is a lytic yersiniophage that can propagate naturally in different Yersinia species carrying the correct lipopolysaccharide receptor. This large-tailed phage has deoxyuridine (dU) instead of thymidine in its DNA. In this study, we determined the genomic sequence of phage ϕR1-3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01783-12
更新日期:2012-12-01 00:00:00
abstract::Virus entry into host cells is a complex process that is largely regulated by access to specific cellular receptors. Human adenoviruses (HAdVs) and many other viruses use cell adhesion molecules such as the coxsackievirus and adenovirus receptor (CAR) for attachment to and entry into target cells. These molecules are ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00542-20
更新日期:2020-07-01 00:00:00
abstract:UNLABELLED:Since the 1960s, simian hemorrhagic fever virus (SHFV; Nidovirales, Arteriviridae) has caused highly fatal outbreaks of viral hemorrhagic fever in captive Asian macaque colonies. However, the source(s) of these outbreaks and the natural reservoir(s) of this virus remain obscure. Here we report the identifica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02203-14
更新日期:2014-11-01 00:00:00
abstract::The ability of the 3A protein of coxsackievirus B (CVB) to inhibit protein secretion was investigated for this study. Here we show that the ectopic expression of CVB 3A blocked the transport of both the glycoprotein of vesicular stomatitis virus, a membrane-bound secretory marker, and the alpha-1 protease inhibitor, a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.8.5163-5173.2005
更新日期:2005-04-01 00:00:00
abstract::Human cytomegalovirus (HCMV) productive replication in vitro is most often studied in fibroblasts. In vivo, fibroblasts amplify viral titers, but transmission and pathogenesis require the infection of other cell types, most notably epithelial cells. In vitro, the study of HCMV infection of epithelial cells has been al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00903-18
更新日期:2018-07-31 00:00:00
abstract::The RNA of infectious bursal disease virus was reexamined in a detailed analysis. It could be established that its genome consists of two segments of double-stranded RNA. The RNA is RNase resistant and has a sedimentation coefficient of 14S and a buoyant density of 1.62 g/ml. The purine/pyrimidine ratio is nearly 1; t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.3.584-589.1979
更新日期:1979-09-01 00:00:00