Abstract:
:Understanding the microbiome of ticks in Australia is of considerable interest given the ongoing debate over whether Lyme disease and its causative agent, the bacterium Borrelia burgdorferisensu lato, are present in Australia. The diversity of bacteria infecting Australian ticks has been studied using both culture- and metagenomics-based techniques. However, little is known about the virome of Australian ticks, including whether this includes viruses with the potential to infect mammals. We used a meta-transcriptomics approach to reveal the diversity and evolution of viruses from Australian ticks collected from two locations on the central east coast of Australia, including metropolitan Sydney. From this we identified 19 novel RNA viruses belonging to 12 families, as well as 1 previously described RNA virus. The majority of these viruses were related to arthropod-associated viruses, suggesting that they do not utilize mammalian hosts. However, two novel viruses discovered in ticks feeding on bandicoot marsupials clustered closely within the mammal-associated hepacivirus and pestivirus groups (family Flaviviridae). Another bandicoot tick yielded a novel coltivirus (family Reoviridae), a group of largely tick-associated viruses containing the known human pathogen Colorado tick fever virus and its relative, Eyach virus. Importantly, our transcriptomic data provided no evidence for the presence of B. burgdorferisensu lato in any tick sample, providing further evidence against the presence of Lyme disease in Australia. In sum, this study reveals that Australian ticks harbor a diverse virome, including some viruses that merit additional screening in the context of emerging infectious disease.IMPORTANCE Each year a growing number of individuals along the east coast of Australia experience debilitating disease following tick bites. As there is no evidence for the presence of the causative agent of Lyme disease, Borrelia burgdorferisensu lato, in Australian ticks, the etiological basis of this disease syndrome remains controversial. To characterize the viruses associated with Australian ticks, particularly those that might be associated with mammalian infection, we performed unbiased RNA sequencing on 146 ticks collected across two locations along the coast of New South Wales, Australia. This revealed 19 novel RNA viruses from a diverse set of families. Notably, three of these viruses clustered with known mammalian viruses, including a novel coltivirus that was related to the human pathogen Colorado tick fever virus.
journal_name
J Viroljournal_title
Journal of virologyauthors
Harvey E,Rose K,Eden JS,Lo N,Abeyasuriya T,Shi M,Doggett SL,Holmes ECdoi
10.1128/JVI.01358-18subject
Has Abstractpub_date
2019-01-17 00:00:00issue
3eissn
0022-538Xissn
1098-5514pii
JVI.01358-18journal_volume
93pub_type
杂志文章abstract::To assess the RNA helicase activity of hepatitis C virus (HCV) nonstructural protein 3 (NS3), a polypeptide encompassing amino acids 1175 to 1657, which cover only the putative helicase domain, was expressed in Escherichia coli by a pET expression vector. The protein was purified to near homogeneity and assayed for RN...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8477-8484.1996
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abstract::HIV circumvents HLA class I-restricted CD8+ T-cell responses through selection of escape mutations that leave characteristic mutational "footprints," also known as HLA-associated polymorphisms (HAPs), on HIV sequences at the population level. While many HLA footprints are universal across HIV subtypes and human popula...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01128-17
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abstract::The great majority of viral mRNAs in mouse C127 cells transformed by bovine papillomavirus type 1 (BPV) have a common 3' end at the early polyadenylation site which is 23 nucleotides (nt) downstream of a canonical poly(A) consensus signal. Twenty percent of BPV mRNA from productively infected cells bypasses the early ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7705-7710.1993
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abstract::The binding of cyclophilin A (CypA) to the human immunodeficiency virus type 1 (HIV-1) capsid protein (CA protein) is required soon after virus entry into natural target cells. In Jurkat T lymphocytes, disrupting CypA-CA interaction either by cyclosporine (Cs) treatment or by alteration (e.g., P90A) of the CA inhibits...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.1.1-7.1979
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.2.405-412.1983
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journal_title:Journal of virology
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abstract::Herpes simplex virus (HSV) latency in neurons remains poorly understood, and the heterogeneity of the sensory nervous system complicates mechanistic studies. In this study, we used primary culture of adult trigeminal ganglion (TG) mouse neurons in microfluidic devices and an in vivo model to examine the subtypes of se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00430-18
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1620-1621.1973
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2001-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.3520-3523.1998
更新日期:1998-05-01 00:00:00
abstract::Viral manipulation of cellular proteins allows viruses to suppress host defenses and generate infectious progeny. Due to the linear double-stranded DNA nature of the adenovirus genome, the cellular DNA damage response (DDR) is considered a barrier to successful infection. The adenovirus genome is packaged with protein...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01089-17
更新日期:2017-09-27 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01719-08
更新日期:2009-01-01 00:00:00
abstract::We have been investigating the first viral genes to be transcribed in Spodoptera frugiperda cells after the infection with Autographa california nuclear polyhedrosis virus in order to identify regulatory proteins required for the activation of early and/or late gene transcription. By screening a cDNA library from poly...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.753-758.1993
更新日期:1993-02-01 00:00:00
abstract::BST-2/CD317/tetherin is a host factor that inhibits the release of HIV-1 and other unrelated viruses. A current model proposes that BST-2 physically tethers virions to the surface of virus-producing cells. The HIV-1-encoded Vpu protein effectively antagonizes the activity of BST-2. How Vpu accomplishes this task remai...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02080-10
更新日期:2011-03-01 00:00:00
abstract::The expression of Marek's disease virus (MDV) transcripts and protein products was investigated in reticuloendotheliosis virus-transformed avian T-lymphoblastoid cell line RECC-CU91, which was superinfected with MDV. The presence of MDV in the superinfected cell line, renamed RECC-CU210, was demonstrated by Southern h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7239-7244.1992
更新日期:1992-12-01 00:00:00
abstract::The hepadnavirus reverse transcriptase is a multifunction enzyme. In addition to its role in DNA synthesis, the polymerase is required for RNA packaging and also functions as the primer for minus-strand DNA synthesis. Previously, we demonstrated that the protein-priming activity of the polymerase requires a viral RNA ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.12.8437-8442.1994
更新日期:1994-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.5256-5261.1998
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abstract::A novel phage lambda mutation, called dc10, which interferes with proper lambda head assembly has been isolated and characterized. Phage lambda carrying this mutation is (i) unable to form plaques at 30 or 37 degrees C but does so at 42 degrees C and (ii) unable to form plaques at 42 degrees C on pN-constitutive hosts...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.2.782-788.1979
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abstract:UNLABELLED:Influenza A virus requires ongoing cellular transcription to carry out the cap-snatching process. Chromatin remodelers modify chromatin structure to produce an active or inactive conformation, which enables or prevents the recruitment of transcriptional complexes to specific genes; viral transcription thus d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00053-16
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00730-12
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abstract::In contrast to humans, several primate species are believed to have harbored simian immunodeficiency viruses (SIVs) since ancient times. In particular, the geographically dispersed species of African green monkeys (AGMs) are all infected with highly diversified SIVagm viruses at high prevalences (greater than 50% of s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.18.8449-8460.2001
更新日期:2001-09-01 00:00:00
abstract::Subgenomic RNAs of both polarities corresponding to rice hoja blanca virus (RHBV) ambisense RNA4 were detected in RHBV-infected rice tissues. Total RNA extracted from RHBV-infected and noninfected rice tissues and RNA4 purified from RHBV ribonucleoprotein particles were used as templates for primer extension studies. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1951-1954.1995
更新日期:1995-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01205-09
更新日期:2009-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.1.27-33.1975
更新日期:1975-07-01 00:00:00
abstract::Among the five serine incorporator (SERINC) family members, SERINC5 (Ser5) was reported to strongly inhibit HIV-1 replication, which is counteracted by Nef. Ser5 produces 5 alternatively spliced isoforms: Ser5-001 has 10 putative transmembrane domains, whereas Ser5-004, -005, -008a, and -008b do not have the last one....
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2017-04-28 00:00:00
abstract::The protein kinase inhibitor H7 blocks influenza virus replication, inhibits production of the matrix protein (M1), and leads to a retention of the viral ribonucleoproteins (vRNPs) in the nucleus at late times of infection (K. Martin and A. Helenius, Cell 67:117-130, 1991). We show here that production of assembled vR...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.4.1781-1786.2000
更新日期:2000-02-01 00:00:00
abstract::Cellular and viral factors participate in the replication cycle of rotavirus. We report that the guanine nucleotide exchange factor GBF1, which activates the small GTPase Arf1 to induce COPI transport processes, is required for rotavirus replication since knocking down GBF1 expression by RNA interference or inhibiting...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01062-19
更新日期:2019-09-12 00:00:00
abstract::The ectopic overexpression of Bcl-2 restricts both influenza A virus-induced apoptosis and influenza A virus replication in MDCK cells, thus suggesting a role for Bcl-2 family members during infection. Here we report that influenza A virus cannot establish an apoptotic response without functional Bax, a downstream tar...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02672-08
更新日期:2009-08-01 00:00:00
abstract::As a subunit of both the P-L polymerase complex and the P-N assembly complex, the vesicular stomatitis virus (VSV) P protein plays a pivotal role in transcription and replication of the viral genome. Constitutive phosphorylation of this protein is currently thought to be essential for formation of the P-L complex. We ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4538-4548.1996
更新日期:1996-07-01 00:00:00