Abstract:
:The ectopic overexpression of Bcl-2 restricts both influenza A virus-induced apoptosis and influenza A virus replication in MDCK cells, thus suggesting a role for Bcl-2 family members during infection. Here we report that influenza A virus cannot establish an apoptotic response without functional Bax, a downstream target of Bcl-2, and that both Bax and Bak are directly involved in influenza A virus replication and virus-induced cell death. Bak is substantially downregulated during influenza A virus infection in MDCK cells, and the knockout of Bak in mouse embryonic fibroblasts yields a dramatic rise in the rate of apoptotic death and a corresponding increase in levels of virus replication, suggesting that Bak suppresses both apoptosis and the replication of virus and that the virus suppresses Bak. Bax, however, is activated and translocates from the cytosol to the mitochondria; this activation is required for the efficient induction of apoptosis and virus replication. The knockout of Bax in mouse embryonic fibroblasts blocks the induction of apoptosis, restricts the infection-mediated activation of executioner caspases, and inhibits virus propagation. Bax knockout cells still die but by an alternative death pathway displaying characteristics of autophagy, similarly to our previous observation that influenza A virus infection in the presence of a pancaspase inhibitor leads to an increase in levels of autophagy. The knockout of Bax causes a retention of influenza A virus NP within the nucleus. We conclude that the cell and virus struggle to control apoptosis and autophagy, as appropriately timed apoptosis is important for the replication of influenza A virus.
journal_name
J Viroljournal_title
Journal of virologyauthors
McLean JE,Datan E,Matassov D,Zakeri ZFdoi
10.1128/JVI.02672-08subject
Has Abstractpub_date
2009-08-01 00:00:00pages
8233-46issue
16eissn
0022-538Xissn
1098-5514pii
JVI.02672-08journal_volume
83pub_type
杂志文章abstract:UNLABELLED:In Kaposi's sarcoma-associated herpesvirus (KSHV), poly(ADP-ribose) polymerase 1 (PARP-1) acts as an inhibitor of lytic replication. Here, we demonstrate that KSHV downregulated PARP-1 upon reactivation. The viral processivity factor of KSHV (PF-8) interacted with PARP-1 and was sufficient to degrade PARP-1 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00559-15
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.21.13705-13713.2005
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01787-13
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11596-11604.2002
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abstract::Cauliflower mosaic virus (CaMV) forms two types of inclusion bodies within infected plant cells: numerous virus factories, which are the sites for viral replication and virion assembly, and a single transmission body (TB), which is specialized for virus transmission by aphid vectors. The TB reacts within seconds to ap...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01883-13
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.1.208-214.1984
更新日期:1984-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.21.11786-11797.2004
更新日期:2004-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.2157-2164.2003
更新日期:2003-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5030-5036.1989
更新日期:1989-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.5613-5620.1999
更新日期:1999-07-01 00:00:00
abstract::Sterile alpha motif domain- and HD domain-containing protein 1 (SAMHD1) is a deoxynucleoside triphosphohydrolase that restricts the replication of lentiviruses in myeloid cells by hydrolyzing the cellular deoxynucleotide triphosphates to a level below that which is required for reverse transcription. Human immunodefic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01657-12
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.680-682.1984
更新日期:1984-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.10.6667-6671.1994
更新日期:1994-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00771-10
更新日期:2010-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.2365-2375.1999
更新日期:1999-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.12.4745-4751.1988
更新日期:1988-12-01 00:00:00
abstract::We provide here new insights into rotavirus (RRV) pathogenicity by showing that RRV infection promotes structural and functional injuries localized at the tight junctions (TJ) in the cell-cell junctional complex of cultured polarized human intestinal Caco-2 cells forming monolayers. RRV infection resulted in a progres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.10.4645-4651.2000
更新日期:2000-05-01 00:00:00
abstract::To evaluate the possibility of producing transducible replication-defective hepadnaviruses, cloned mutant duck hepatitis B virus genomes were tested both for virus antigen production and viral DNA synthesis following transfection into the human hepatoma cell line HuH7. Deletion of a cis-acting 12-nucleotide sequence i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.642-650.1990
更新日期:1990-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2133-2139.1995
更新日期:1995-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.2.241-249.1971
更新日期:1971-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01893-17
更新日期:2018-02-12 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) (also called human herpesvirus 8) is consistently found in Kaposi's sarcoma lesions and in body-cavity-based lymphomas. A 17-kb KSHV lambda clone was obtained directly from a Kaposi's sarcoma lesion. DNA sequence analysis of this clone identified an open reading frame whi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.1984-1991.1997
更新日期:1997-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.3.423-430.1972
更新日期:1972-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02433-10
更新日期:2011-07-01 00:00:00
abstract::The feline immunodeficiency virus (FIV) cat model is extensively used to investigate possible vaccination approaches against AIDS in humans. Although consistent levels of protection have been achieved with FIV, as with other model systems, by immunizing with whole inactivated virus or fixed infected cells, the mechani...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.1-10.1999
更新日期:1999-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.3.921-924.1987
更新日期:1987-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00126-09
更新日期:2009-07-01 00:00:00
abstract::The interaction of the human immunodeficiency virus (HIV) Gag protein with the plasma membrane of a cell is a critical event in the assembly of HIV particles. The matrix protein region (MA) of HIV type 1 (HIV-1) Pr55Gag has previously been demonstrated to confer membrane-binding properties on the precursor polyprotein...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.6582-6592.1997
更新日期:1997-09-01 00:00:00
abstract::Human papillomavirus (HPV) type 16 E6 (16E6) is an oncogenic, multifunctional nuclear protein that induces p53 degradation and perturbs normal cell cycle control, leading to immortalization and transformation of infected keratinocytes and epithelial cells. Although it is unclear how 16E6 disrupts the epigenetic profil...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.24.13232-13247.2003
更新日期:2003-12-01 00:00:00