Abstract:
:Synthetic peptides of increasing length and corresponding in sequence to the C-terminal end of the HA1 molecule of influenza virus were constructed and examined for their immunogenic and antigenic properties. Peptides containing at least the four C-terminal amino acids, when coupled to keyhole limpet hemocyanin, were capable of eliciting antibody in BALB/c mice that bound to the 24-residue parent peptide H3 HA1 (305 to 328). In the absence of a carrier, the C-terminal decapeptide was the shortest peptide capable of eliciting antibody. The specificity of this antibody was indistinguishable from that of a monoclonal antibody to the parent peptide which recognizes an epitope encompassed by the C-terminal seven residues. All peptides containing at least the C-terminal four residues were able to inhibit completely the binding of this monoclonal antibody to the parent peptide. Taken together, these results indicate that (i) the tetrapeptide is capable of eliciting specific antibody when coupled to a carrier, (ii) this tetrapeptide possesses all of the antigenic information necessary to occupy the paratope of a monoclonal antibody elicited by the longer parent peptide, and (iii) the decapeptide contains all of the information necessary to elicit a specific immune response and therefore carries an epitope recognized by T cells as well as one recognized by B cells.
journal_name
J Viroljournal_title
Journal of virologyauthors
Tang XL,Tregear GW,White DO,Jackson DCdoi
10.1128/JVI.62.12.4745-4751.1988subject
Has Abstractpub_date
1988-12-01 00:00:00pages
4745-51issue
12eissn
0022-538Xissn
1098-5514journal_volume
62pub_type
杂志文章abstract::Respiratory syncytial virus (RSV) is the major cause of acute bronchiolitis in infancy, a syndrome characterized by wheezing, respiratory distress, and the pathologic findings of peribronchial mononuclear cell infiltration and release of inflammatory mediators by basophil and eosinophil leukocytes. Composition and act...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.4756-4764.1998
更新日期:1998-06-01 00:00:00
abstract::Human papillomavirus type 18 (HPV18) and HPV45 account for approximately 20% of all cervix cancers. We show that HPV18, HPV45, and the recently discovered HPV97 comprise a clade sharing a most recent common ancestor within HPV alpha7 species. Variant lineages of these HPV types were classified by sequence analysis of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02068-08
更新日期:2009-02-01 00:00:00
abstract::The majority of people infected with hepatitis C virus (HCV) fail to generate or maintain a T-cell response effective for viral clearance. Evidence from murine chronic viral infections shows that expression of the coinhibitory molecule PD-1 predicts CD8+ antiviral T-cell exhaustion and may contribute to inadequate pat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02021-06
更新日期:2007-03-01 00:00:00
abstract::Mosquito-borne alphaviruses, which replicate alternately and obligately in mosquitoes and vertebrates, appear to experience lower rates of evolution than do many RNA viruses that replicate solely in vertebrates. This genetic stability is hypothesized to result from the alternating host cycle, which constrains evolutio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.14253-14260.2005
更新日期:2005-11-01 00:00:00
abstract::In December 2016, a low-pathogenic avian influenza (LPAI) A(H7N2) virus was identified to be the causative source of an outbreak in a cat shelter in New York City, which subsequently spread to multiple shelters in the states of New York and Pennsylvania. One person with occupational exposure to infected cats became in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00672-17
更新日期:2017-07-12 00:00:00
abstract::Antiviral effects were characterized for two oligodeoxyribonucleoside methylphosphonates synthesized in an antisense (3'-TCTTAACC-5') or a sense (5'-AGAATTGG-3') orientation, based on the RNA sequence of the first splice acceptor site of the tat-3 gene of human immunodeficiency virus (HIV) (5'...AGAAUUGG...3'). The de...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.10.3914-3917.1988
更新日期:1988-10-01 00:00:00
abstract::Two of 25 healthy pet sooty mangabey (SM) monkeys (Cercocebus atys) living in West Africa were seropositive by immunoblot when surveyed for antibody to simian immunodeficiency virus of macaques (SIVmac). SIVsmLIB1 was isolated from one of the pet sooty mangabeys. Nucleotide sequence data showed that this isolate is a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4480-4485.1991
更新日期:1991-08-01 00:00:00
abstract::cpts530, a candidate live-virus vaccine, is an attenuated strain of human respiratory syncytial virus (RSV). It was derived by subjecting a cold-passaged (cp) strain of RSV to a single round of chemical mutagenesis. cpts530 is a temperature-sensitive (ts) mutant that is attenuated in mice and chimpanzees, and its ts p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.8.5814-5819.1997
更新日期:1997-08-01 00:00:00
abstract::Murine gammaherpesvirus 68 (gammaHV68) provides an important experimental model for understanding mechanisms of immune control of the latent human gammaherpesviruses. Antiviral CD8 T cells play a key role throughout three separate phases of the infection: clearance of lytic virus, control of the latency amplification ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02229-09
更新日期:2010-03-01 00:00:00
abstract::Herpesvirus ateles is an agent indigenous to spider monkeys (Ateles spp.) and causes fulminant lymphomas in various New World primates. Structural and genetic relatedness led to the classification of this virus as a member of the genus Rhadinovirus. It is most closely related to Herpesvirus saimiri. The 108,409-bp lig...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.2.1033-1037.2000
更新日期:2000-01-01 00:00:00
abstract::Rabies, as the oldest known infectious disease, remains a serious threat to public health worldwide. The eukaryotic cytosolic chaperonin TRiC/CCT complex facilitates the folding of proteins through ATP hydrolysis. Here, we investigated the expression, cellular localization, and function of neuronal CCTγ during neurotr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03186-12
更新日期:2013-07-01 00:00:00
abstract::V2/V3 conformational epitope antibodies that broadly neutralize HIV-1 (PG9 and PG16) have been recently described. Since an elicitation of previously known broadly neutralizing antibodies has proven elusive, the induction of antibodies with such specificity is an important goal for HIV-1 vaccine development. A critica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05045-11
更新日期:2011-10-01 00:00:00
abstract::Intragastric inoculation with whole-virion vaccine of inactivated influenza virus resulted in production of hemagglutinin (HA)-specific immunoglobulin A (IgA) and IgG both in lung lavage fluids and in serum samples of mice. HA-specific IgA was the predominant isotypic antibody secreted in the lung lavage fluids (avera...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2150-2154.1987
更新日期:1987-07-01 00:00:00
abstract::NSP5 (NS26), the product of rotavirus gene 11, is a phosphoprotein whose role in the virus replication cycle is unknown. To gain further insight into its function, we obtained monoclonal antibodies against the baculovirus-expressed protein. By immunoprecipitation and immunoblotting experiments, we showed that (i) NSP5...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.34-41.1997
更新日期:1997-01-01 00:00:00
abstract::We previously described a human immunodeficiency virus type 1 (HIV-1) envelope mutant that introduces a disulfide bridge between the gp120 surface proteins and gp41 transmembrane proteins (J. M. Binley, R. W. Sanders, B. Clas, N. Schuelke, A. Master, Y. Guo, F. Kajumo, D. J. Anselma, P. J. Maddon, W. C. Olson, and J. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.10.5678-5684.2003
更新日期:2003-05-01 00:00:00
abstract::DNA vaccines have recently emerged at the forefront of approaches to harness the immune system in the prevention and treatment of viral infections, as well as the prevention and treatment of cancers. However, these vaccines suffer from limited efficacy since they often fail to produce significant antigen-specific CD8(...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01954-09
更新日期:2010-03-01 00:00:00
abstract::Simian immunodeficiency virus (SIV) has been shown to progress through a number of changes that lead to the emergence of pathogenic viral variants in macaques initially infected with a mildly cytopathic variant, SIVMneCL8. One of these late-stage isolates, SIVMne170, replicates to high levels in vivo and causes a rapi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.24.15091-15098.2005
更新日期:2005-12-01 00:00:00
abstract::We analyzed peripheral blood mononuclear cells from 19 asymptomatic seropositive pregnant women from the district of Gulu in northern Uganda. A 700-bp fragment of the human immunodeficiency virus type 1 (HIV-1) env gene, including the V3-V5 region, was successfully amplified by PCR from 10 samples (52.6%) and was subs...
journal_title:Journal of virology
pub_type: 杂志文章,多中心研究
doi:10.1128/JVI.69.12.7971-7981.1995
更新日期:1995-12-01 00:00:00
abstract::Mature nonstructural protein-15 (nsp15) from the severe acute respiratory syndrome coronavirus (SARS-CoV) contains a novel uridylate-specific Mn2+-dependent endoribonuclease (NendoU). Structure studies of the full-length form of the obligate hexameric enzyme from two CoVs, SARS-CoV and murine hepatitis virus, and its ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02817-06
更新日期:2007-06-01 00:00:00
abstract::We investigated the cis-acting sequences involved in termination of vesicular stomatis virus mRNA synthesis by using bicistronic genomic analogs. All of the cis-acting signals necessary for termination reside within the first 13 nucleotides of the 23-nucleotide conserved gene junction. This 13-nucleotide termination s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8718-8725.1997
更新日期:1997-11-01 00:00:00
abstract::Zika virus (ZIKV) has emerged as a cause of congenital brain anomalies and a range of placenta-related abnormalities, highlighting the need to unveil the modes of maternal-fetal transmission. The most likely route of vertical ZIKV transmission is via the placenta. The earliest events of ZIKV transmission in the matern...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01905-16
更新日期:2017-01-31 00:00:00
abstract::In this study, we show that human cytomegalovirus DNA synthesis is inhibited in infected confluent human embryonic lung cells treated with the DNA-intercalative topoisomerase II inhibitor 4-9'-(acridinylamino)methanesulfon-m-anisidide (m-AMSA). Similar inhibitory effects were observed with VM-26, a nonintercalative to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.12.4797-4800.1988
更新日期:1988-12-01 00:00:00
abstract::Interactions between host factors and the viral replication complex play important roles in host adaptation and regulation of influenza virus replication. A cellular protein, nuclear factor 90 (NF90), was copurified with H5N1 viral nucleoprotein (NP) from human cells in which NP was transiently expressed and identifie...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00735-09
更新日期:2009-08-01 00:00:00
abstract::Previous recombinant Epstein-Barr virus molecular genetic experiments with specifically mutated LMP1 genes indicate that LMP1 is essential for primary B-lymphocyte growth transformation and that the amino-terminal cytoplasmic and first transmembrane domains are together an important mediator of transformation. EBV rec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.7.4369-4376.1994
更新日期:1994-07-01 00:00:00
abstract::The role of glycosylation and of disulfide bonds in the formation of the native structure of the Sendai virus hemagglutinin-neuraminidase (HN) and fusion (F0) glycoproteins was studied. In contrast to the HN and F0 synthesized in vivo, the proteins made from pSP6 transcripts in reticulocyte lysates, whether glycosylat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.2.892-900.1989
更新日期:1989-02-01 00:00:00
abstract::Anti-VPg immunoprecipitable RNA labeled in vitro during a poliovirus RNA polymerase reaction was formed by the elongation of VPg-containing template fragments rather than by initiation with VPg. The reaction was dependent on a host factor (terminal uridylyl transferase). The incorporation of labeled UTP could be detec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.1.212-215.1986
更新日期:1986-04-01 00:00:00
abstract::A recombinant vesicular stomatitis virus (VSV-PeGFP-M-MmRFP) encoding enhanced green fluorescent protein fused in frame with P (PeGFP) in place of P and a fusion matrix protein (monomeric red fluorescent protein fused in frame at the carboxy terminus of M [MmRFP]) at the G-L gene junction, in addition to wild-type (wt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01668-08
更新日期:2009-03-01 00:00:00
abstract::The emergence in 1997 and continuance today of a highly lethal H5N1 avian influenza virus (AIV) causing human disease has raised concern about an impending pandemic and the need for a vaccine to prepare for such an occurrence. We previously generated an efficacious vesicular stomatitis virus (VSV)-based AIV vaccine ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02637-09
更新日期:2010-05-01 00:00:00
abstract::Rabies virus (RV) of the Rhabdoviridae family grows in alpha/beta interferon (IFN)-competent cells, suggesting the existence of viral mechanisms preventing IFN gene expression. We here identify the viral phosphoprotein P as the responsible IFN antagonist. The critical involvement of P was first suggested by the observ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.12.7673-7681.2005
更新日期:2005-06-01 00:00:00
abstract::To assess the heterogeneity of cytotoxic T lymphocytes (CTLs) directed against viral epitopes, we studied six class I major histocompatibility complex-restricted (H-2Db) CTL clones that recognize the same 9-amino-acid immunodominant epitope, amino acids 278 to 286 from envelope glycoprotein 2 (GP2) of lymphocytic chor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.1845-1851.1989
更新日期:1989-05-01 00:00:00