Abstract:
:DNA vaccines have recently emerged at the forefront of approaches to harness the immune system in the prevention and treatment of viral infections, as well as the prevention and treatment of cancers. However, these vaccines suffer from limited efficacy since they often fail to produce significant antigen-specific CD8(+) T-cell responses. We report here a novel concept for DNA vaccine design that exploits the unique and powerful ability of viral fusogenic membrane glycoproteins (FMGs) to couple concentrated antigen transfer to dendritic cells (DCs) with local induction of the acute inflammatory response. Intramuscular administration into mice by electroporation technology of a plasmid containing the FMG gene from vesicular stomatitis virus (VSV-G)-together with DNA encoding the E7 protein of human papillomavirus type 16, a model cervical cancer antigen-elicited robust E7-specific CD8(+) T-cell responses, as well as therapeutic control of E7-expressing tumors. This effect could potentially be mediated through the immunogenic form of cellular fusion and necrosis induced by VSV-G, which in a concerted fashion provokes leukocyte infiltration into the inoculation site, enhances cross-presentation of antigen to DCs, and stimulates them to mature efficiently. Thus, the incorporation of FMGs into DNA vaccines holds promise for the successful control of viral infections and cancers in the clinic.
journal_name
J Viroljournal_title
Journal of virologyauthors
Mao CP,Hung CF,Kang TH,He L,Tsai YC,Wu CY,Wu TCdoi
10.1128/JVI.01954-09subject
Has Abstractpub_date
2010-03-01 00:00:00pages
2331-9issue
5eissn
0022-538Xissn
1098-5514pii
JVI.01954-09journal_volume
84pub_type
杂志文章abstract::The swine-specific coronavirus transmissible gastroenteritis virus (TGEV) uses pig aminopeptidase-N (pAPN) as a cellular receptor. We showed that the human aminopeptidase-N (hAPN) cannot substitute for pAPN in this respect, although the two enzymes have 80% amino acid sequence identity. In order to map the TGEV bindin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5216-5224.1994
更新日期:1994-08-01 00:00:00
abstract::In vitro studies have suggested an important role for the minor capsid protein (VP1) unique region and the junction between VP1 and the major capsid protein (VP2) in the neutralizing immune response to B19 parvovirus. We investigated the role of the NH2-terminal region of the major structural protein in capsid structu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.10.6567-6571.1995
更新日期:1995-10-01 00:00:00
abstract::In vitro transcription by vesicular stomatitis virus nucleocapsids is inhibited by enzymatic dephosphorylation of the NS protein. We provide evidence that specific, partial dephosphorylation of NS molecules is the only detectable change in nucleocapsids treated with bacterial alkaline phosphatase under conditions that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.1.104-112.1982
更新日期:1982-07-01 00:00:00
abstract::The induction of alpha/beta interferon (IFN-alpha/beta) genes constitutes one of the first responses of the cell to virus infection. The IFN-beta gene is constitutively repressed in uninfected cells and is transiently activated after virus infection. In this work we demonstrate that histone deacetylation regulates the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.7.3444-3452.2001
更新日期:2001-04-01 00:00:00
abstract::Simian virus 40 (SV40) infection of human diploid cells failed to cause an enhanced production of thymidine kinase during the first 10 days after infection. Thymidine kinase activities from extracts of SV40-transformed cultures (human or simian) were considerably higher than the activity levels in extracts from the no...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.5.912-919.1967
更新日期:1967-10-01 00:00:00
abstract::Defective interfering particles (DIPs) of equine herpesvirus 1 (EHV-1; Kentucky A strain) mediate persistent infection. DNA sequences at the L terminus, which contain the UL2 gene (homolog of UL55 of herpes simplex virus type 1 and open reading frame 3 of varicella-zoster virus) of standard EHV-1, have been shown to b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2255-2265.1993
更新日期:1993-04-01 00:00:00
abstract::The 2009 H1N1 influenza pandemic provided an opportunity to study human virus-specific T cell responses after infection with a novel influenza virus against which limited humoral immunity existed in the population. Here we describe the magnitude, kinetics, and nature of the virus-specific T cell response using intrace...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05204-11
更新日期:2011-11-01 00:00:00
abstract::Callitrichid hepatitis is an infection of New World primates caused by an arenavirus, currently referred to as callitrichid hepatitis virus, that is closely related to lymphocytic choriomeningitis virus (LCMV). We have cloned and sequenced the GP-C gene of callitrichid hepatitis virus and found that the cDNA sequence ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1349-1352.1995
更新日期:1995-02-01 00:00:00
abstract::The E7 oncoprotein of human papillomavirus 16 (HPV16) transforms basal and suprabasal cervical epithelial cells and is a tumor-specific antigen in cervical carcinoma, to which immunotherapeutic strategies aimed at cytotoxic T-lymphocyte (CTL) induction are currently directed. By quantifying major histocompatibility co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.5985-5997.2001
更新日期:2001-07-01 00:00:00
abstract::Lassa fever (LF) is a zoonotic viral hemorrhagic fever caused by Lassa virus (LASV), which is endemic to West African countries. Previous studies have suggested an important role for T-cell-mediated immunopathology in LF pathogenesis, but the mechanisms by which T cells influence disease severity and outcome are not w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01367-20
更新日期:2020-10-14 00:00:00
abstract::Extracts of competent cells of Bacillus subtilis exhibited nuclease activity on radioactively labeled cell deoxyribonucleic acid (DNA). The activity was not decreased when Mg was omitted from the reaction mixture but was decreased to zero by addition of ethylenediaminetetraacetic acid. Of the other metals tested, only...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.6.749-752.1971
更新日期:1971-06-01 00:00:00
abstract::TNPO3 is a nuclear importer required for HIV-1 infection. Here, we show that depletion of TNPO3 leads to an HIV-1 block after nuclear import but prior to integration. To investigate the mechanistic requirement of TNPO3 in HIV-1 infection, we tested the binding of TNPO3 to the HIV-1 core and found that TNPO3 binds to t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00451-12
更新日期:2012-05-01 00:00:00
abstract::Humans develop polyclonal, serotype-specific neutralizing antibody responses after dengue virus (DENV) infection. Many mouse antibodies that neutralize DENV bind to the lateral ridge or A strand epitopes on domain III of the viral envelope (EDIII) protein. It has been assumed that these epitopes are also the main targ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06871-11
更新日期:2012-04-01 00:00:00
abstract::Recently it has been shown that kaposica, an immune evasion protein of Kaposi's sarcoma-associated herpesvirus, inactivates complement by acting on C3-convertases by accelerating their decay as well as by acting as a cofactor in factor I-mediated inactivation of their subunits C3b and C4b. Here, we have mapped the fun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5850-5856.2005
更新日期:2005-05-01 00:00:00
abstract::Although combination therapy allows the suppression of human immunodeficiency virus type 1 (HIV-1) viremia to undetectable levels, eradication has not been achieved because the virus persists in cellular reservoirs, particularly the latent reservoir in resting CD4(+) T lymphocytes. We previously established a simian i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01396-06
更新日期:2007-02-01 00:00:00
abstract::The movement protein (MP) of Tobacco mosaic virus mediates the cell-to-cell transport of viral RNA through plasmodesmata, cytoplasmic cell wall channels for direct cell-to-cell communication between adjacent cells. Previous in vivo studies demonstrated that the RNA transport function of the protein correlates with its...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00254-06
更新日期:2006-06-01 00:00:00
abstract::We are using site-directed mutagenesis of single viral genes to identify and analyze the genetic determinants of human and simian immunodeficiency virus pathogenicity. In a first approach, we have constructed a series of simian immunodeficiency virus SIVmac nef mutants by partial deletion and insertions in the nef gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5237-5243.1991
更新日期:1991-10-01 00:00:00
abstract::We investigated how the broadly neutralizing monoclonal antibody 2F5 affects the human immunodeficiency virus type 1 envelope glycoprotein as it undergoes receptor-induced conformational changes and show that 2F5 binds both native and fusion-intermediate conformations, suggesting inhibition of a late step in virus ent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2627-2631.2004
更新日期:2004-03-01 00:00:00
abstract::Simian virus 40 (SV40) growth on rhesus kidney cells and on the T-22 line of SV40-transformed green monkey kidney (GMK) cells is largely limited by the low plating efficiency of SV40 on these cells. In addition, a fraction of the rhesus kidney and T-22 cells are resistant to infection by SV40. Nevertheless, 72-h viral...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.1.48-57.1976
更新日期:1976-04-01 00:00:00
abstract::Measles virus has two glycoproteins. The larger glycoprotein (HA) is composed of 76,000-dalton subunits that are bound by disulfide bonds. The smaller glycoprotein (F) appears to contain a glucosamine-rich portion that is linked to an unglycosylated protein by disulfide bonds. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.2.687-692.1978
更新日期:1978-02-01 00:00:00
abstract::Rotavirus NSP1 has been shown to function as an E3 ubiquitin ligase that mediates proteasome-dependent degradation of interferon (IFN) regulatory factors (IRF), including IRF3, -5, and -7, and suppresses the cellular type I IFN response. However, the effect of rotavirus NSP1 on viral replication is not well defined. P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00585-09
更新日期:2009-07-01 00:00:00
abstract::A novel phage lambda mutation, called dc10, which interferes with proper lambda head assembly has been isolated and characterized. Phage lambda carrying this mutation is (i) unable to form plaques at 30 or 37 degrees C but does so at 42 degrees C and (ii) unable to form plaques at 42 degrees C on pN-constitutive hosts...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.2.782-788.1979
更新日期:1979-02-01 00:00:00
abstract::The J-F intercistronic region of the genomes of bacteriophages phi X174 and G4 is transcribed but not translated. It contains the potential for a perfectly base-paired hairpin structure, which has been proposed to act as a terminator of transcription or as a regulatory region for mRNA turnover (or both). We measured p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.1.180-185.1983
更新日期:1983-10-01 00:00:00
abstract::The classical swine fever virus (CSFV) live attenuated vaccine C-strain is adaptive to rabbits and attenuated in pigs, in contrast with the highly virulent CSFV Shimen strain. Previously, we demonstrated that P108 and T109 on the E2 glycoprotein (E2P108-T109) in domain I (E2DomainI) rather than R132, S133, and D191 in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01104-20
更新日期:2020-08-17 00:00:00
abstract::Foamy viruses (FVs) generate their Pol protein precursor molecule independently of the Gag protein from a spliced mRNA. This mode of expression raises the question of the mechanism of Pol protein incorporation into the viral particle (capsid). We previously showed that the packaging of (pre)genomic RNA is essential fo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.7005-7013.2005
更新日期:2005-06-01 00:00:00
abstract::Adeno-associated virus (AAV) integrates site specifically into the AAVS1 locus on human chromosome 19. Although recruitment of the AAV nonstructural protein Rep78/68 to the Rep binding site (RBS) on AAVS1 is thought to be an essential step, the mechanism of the site-specific integration, particularly, how the site of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02014-06
更新日期:2007-02-01 00:00:00
abstract::Several domains of CD4 have been suggested to play a critical role in events that follow its binding to the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (gp120-gp41). It has been reported previously that cells expressing a chimeric molecule consisting of the first 177 residues of human CD4 attache...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.11.6469-6475.1993
更新日期:1993-11-01 00:00:00
abstract::Vaccination for human immunodeficiency virus type 1 (HIV-1) remains an elusive goal. Whether an unsuccessful vaccine might not only fail to provoke detectable immune responses but also could actually interfere with subsequent natural immunity upon HIV-1 infection is unknown. We performed detailed assessment of an HIV-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01720-07
更新日期:2008-03-01 00:00:00
abstract::Two different proteins with high affinities for the pteridine ring of folic acid have been used to determine the location of this portion of the folate molecule in the tail plate of T4D and other T-even bacteriophage particles. The two proteins used were (i) antibody specific for folic acid and (ii) the folate-binding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.6.1391-1400.1975
更新日期:1975-12-01 00:00:00
abstract::NSP5 (NS26), the product of rotavirus gene 11, is a phosphoprotein whose role in the virus replication cycle is unknown. To gain further insight into its function, we obtained monoclonal antibodies against the baculovirus-expressed protein. By immunoprecipitation and immunoblotting experiments, we showed that (i) NSP5...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.34-41.1997
更新日期:1997-01-01 00:00:00