Abstract:
:Defective interfering particles (DIPs) of equine herpesvirus 1 (EHV-1; Kentucky A strain) mediate persistent infection. DNA sequences at the L terminus, which contain the UL2 gene (homolog of UL55 of herpes simplex virus type 1 and open reading frame 3 of varicella-zoster virus) of standard EHV-1, have been shown to be highly conserved in all clones of the EHV-1 DIP genome. The UL2 mRNA was characterized by S1 nuclease analyses, which mapped the 5' and 3' termini of the 0.9-kb early UL2 mRNA to approximately 26 and 16 nucleotides downstream of a TTTAAA box and polyadenylation signal, respectively. The UL2 open reading frame, present within both the EHV-1 standard and DIP genomes, was inserted into the transcription expression vector pGEM-3Z to yield constructs pGEML2 and pDIL2, respectively. After in vitro transcription and translation, both constructs yielded a comigrating 23-kDa protein, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Polyclonal antiserum was raised against the UL2 protein by injecting rabbits with a TrpE/UL2 fusion protein expressed from plasmid pATH23L2 in Escherichia coli. The UL2-specific antiserum reacted in Western immunoblot and immunoprecipitation analyses with a 23-kDa polypeptide synthesized in cells infected with standard EHV-1 or DIP-enriched virus. These data also indicated that the UL2 polypeptide was more abundant in DIP-infected cells than in standard EHV-1-infected cells. Results from time course and pulse-chase analyses suggested that the UL2 polypeptide has a rapid turnover rate in DIP-infected cells.
journal_name
J Viroljournal_title
Journal of virologyauthors
Harty RN,Holden VR,O'Callaghan DJdoi
10.1128/JVI.67.4.2255-2265.1993subject
Has Abstractpub_date
1993-04-01 00:00:00pages
2255-65issue
4eissn
0022-538Xissn
1098-5514journal_volume
67pub_type
杂志文章abstract::Human herpesvirus 8 (HHV-8) (also known as Kaposi's sarcoma-associated herpesvirus) encodes a novel noncoding polyadenylated nuclear (PAN) RNA (also known as T1.1 or nut-1) during the early phase of lytic replication. PAN RNA is the most abundant transcript of HHV-8, comprising 80% of total poly(A)-selected transcript...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.7.3129-3140.2001
更新日期:2001-04-01 00:00:00
abstract::The full-length sequence of a satellite RNA (sat-RNA) of Beet black scorch virus isolate X (BBSV-X) was determined. This agent is 615 nucleotides long and lacks extensive sequence homology with its helper virus or with other reported viruses. Purified virus particles contained abundant single-stranded plus-sense monom...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3664-3674.2005
更新日期:2005-03-01 00:00:00
abstract::Transcription of herpesviral late genes is stimulated after the onset of viral DNA replication but otherwise restricted. Late gene expression in gammaherpesviruses requires the coordination of six early viral proteins, termed viral transactivation factors (vTFs). Here, we mapped the organization of this protein comple...
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4211-4215.1991
更新日期:1991-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02229-10
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abstract::Epstein-Barr virus (EBV)-specific T-cell lines generated by repeated stimulation with EBV-immortalized lymphoblastoid B-cell lines (LCL) have been successfully used to treat EBV-associated posttransplant lymphoproliferative disease (PTLD) in hematopoietic stem cell transplant recipients. However, PTLD in solid-organ t...
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pub_type: 杂志文章
doi:10.1128/JVI.02227-07
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abstract::Under the immune pressure of cytotoxic T cells (CTLs), hepatitis B virus (HBV) evolves to accumulate mutations more likely within epitopes to evade immune detection. However, little is known about the specific patterns of the immune pressure-associated HBV mutation of T-cell epitopes and their link to disease progress...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02120-17
更新日期:2018-08-16 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02831-06
更新日期:2007-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.23.13082-13089.2004
更新日期:2004-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.2916-2920.1999
更新日期:1999-04-01 00:00:00
abstract::The host cell receptor for Moloney murine leukemia virus was solubilized from murine L-cell membranes and characterized. In initial studies designed to identify a receptor-rich cell line, different mouse cells were screened for binding to Moloney gp70, the viral envelope glycoprotein which determines host cell-binding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.3.900-908.1986
更新日期:1986-06-01 00:00:00
abstract::The precise roles of gamma interferon-inducible immunoproteasome-associated molecules in generation of cytotoxic T-lymphocyte (CTL) epitopes have yet to be fully elucidated. We describe here a unique epitope derived from the Epstein-Barr virus (EBV) latent membrane protein 2A (LMP2A) presented by HLA-A*2402 molecules....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.2.883-890.2006
更新日期:2006-01-01 00:00:00
abstract::Enzymatic markers and electron microscopy were utilized to determine the cellular origin of the membrane types isolated from type 2 dengue virus-infected BHK cells by discontinuous sucrose gradient centrifugation. The results showed an apparent separation of plasma membrane, smooth and rough endoplasmic reticulum with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.4.1017-1026.1975
更新日期:1975-10-01 00:00:00
abstract:UNLABELLED:Due to a scarcity of immunocompetent animal models for viral hepatitis, little is known about the early innate immune responses in the liver. In various hepatotoxic models, both pro- and anti-inflammatory activities of recruited monocytes have been described. In this study, we compared the effect of liver in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03733-14
更新日期:2015-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.4.460-474.1969
更新日期:1969-10-01 00:00:00
abstract::Detailed phylogenetic analyses were performed to characterize an HIV-1 outbreak among injection drug users (IDUs) in Stockholm, Sweden, in 2006. This study investigated the source and dynamics of HIV-1 spread during the outbreak as well as associated demographic and clinical factors. Seventy Swedish IDUs diagnosed dur...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01413-10
更新日期:2011-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.9755-9762.1998
更新日期:1998-12-01 00:00:00
abstract::A number of human immunodeficiency type 1 (HIV-1)-based vectors have recently been shown to transduce nondividing cells in vivo as well as in vitro. However, if these vectors are to be considered for eventual clinical use, a major consideration is to reduce the probability of unintended generation of replication-compe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.1828-1834.1999
更新日期:1999-03-01 00:00:00
abstract:UNLABELLED:During hepatitis C virus (HCV) infection, broadly neutralizing antibody (bNAb) responses targeting E1E2 envelope glycoproteins are generated in many individuals. It is unclear if these antibodies play a protective or a pathogenic role during chronic infection. In this study, we investigated whether bNAb resp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02669-15
更新日期:2016-04-14 00:00:00
abstract::The glycan shield of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) protein serves as a barrier to antibody-mediated neutralization and plays a critical role in transmission and infection. One of the few broadly neutralizing HIV-1 antibodies, 2G12, binds to a carbohydrate epitope consisting of an array...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6810-6815.1996
更新日期:1996-10-01 00:00:00
abstract::The V1V2 region of HIV-1 gp120 harbors a major vulnerable site targeted by a group of broadly neutralizing monoclonal antibodies (MAbs) such as PG9 through strand-strand recognition. However, this epitope region is structurally polymorphic as it can also form a helical conformation recognized by RV144 vaccine-induced ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01409-16
更新日期:2016-11-28 00:00:00
abstract::A herpes simplex virus type 1 variant [C239(TK-)] harboring a deletion in the thymidine kinase (TK) gene was assessed for capacity to establish latent infections. Outbred Swiss Webster mice were inoculated on both hind footpads, and numbers of neurons expressing latency-associated transcript and amounts of viral DNA i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4976-4978.1989
更新日期:1989-11-01 00:00:00
abstract::A procedure has been developed for the isolation of Newcastle disease virus (NDV) envelope proteins. The two surface glycoproteins and the non-glycosylated membrane protein were solubilized with 2% Triton X-100 and 1 m KCl. Removal of the KCl by dialysis yielded by precipitation a pure preparation of the non-glycosyla...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.2.263-271.1973
更新日期:1973-02-01 00:00:00
abstract::10A1 murine leukemia virus can enter cells by using either of two different cell surface phosphate transport proteins, the gibbon ape leukemia virus receptor Glvr-1 (Pit-1) or the amphotropic retrovirus receptor Ram-1 (Pit-2). Glvr-1 and Ram-1 are widely expressed in different tissues, but the relative amounts of each...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5564-5571.1996
更新日期:1996-08-01 00:00:00
abstract::Replication of flock house virus (FHV) RNA1 and production of subgenomic RNA3 in the yeast Saccharomyces cerevisiae provide a useful tool for the dissection of FHV molecular biology and host-encoded functions involved in RNA replication. The replication template activity of RNA1 can be separated from its coding potent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.3905-3919.2002
更新日期:2002-04-01 00:00:00
abstract::Mouse-adapted dengue type 4 virus (DEN4) strain H241 is highly neurovirulent for mice, whereas its non-mouse-adapted parent is rarely neurovirulent. The genetic basis for the neurovirulence of the mouse-adapted mutant was studied by comparing intratypic chimeric viruses that contained the three structural protein gene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.11.6567-6575.1993
更新日期:1993-11-01 00:00:00
abstract::We have synthesized 32P-labeled hybridization probes from a hyperdivergent region (nucleotides 51 to 392) of the rotavirus gene encoding the VP7 glycoprotein by using the polymerase chain reaction method. Both RNA (after an initial reverse transcription step) and cloned cDNA from human rotavirus serotypes 1 through 4 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.4021-4024.1990
更新日期:1990-08-01 00:00:00
abstract::The phenotypic defects of two mutants of vaccinia virus, the lesions of which map to gene G2R, were characterized in vivo, and the results suggest a role for the G2R protein in viral transcription elongation. Both a temperature-sensitive mutant, Cts56, and an isatin-beta-thiosemicarbazone-dependent deletion mutant, G2...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.47-54.1996
更新日期:1996-01-01 00:00:00
abstract:UNLABELLED:Numerous animal and plant viruses are transmitted by arthropod vectors in a persistent, circulative manner. Tomato yellow leaf curl virus (TYLCV) is transmitted by the sweet potato whitefly Bemisia tabaci. We report here that infection with Rickettsia spp., a facultative endosymbiont of whiteflies, altered T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00071-14
更新日期:2014-05-01 00:00:00