Abstract:
:The ribonucleic acid (RNA) of murine leukemia virus (MLV) Rauscher strain was observed by the aid of electron microscopy with the use of the protein monolayer technique. RNA was observed directly after release from virus particles or after isolation by sedimentation in sucrose density gradients. Molecules were found in an extended linear form. Many of the RNA filaments released by detergent treatment contained curled regions, suggesting the linear filaments were originally coiled within the virus particle. The relationship of the curled areas to the containment of the RNA within the virus particle is discussed, and a mechanism for the inclusion of RNA in the budding virion is proposed. Treatment of the extended MLV-RNA with dimethyl sulfoxide resulted in the collapse of the molecule forming a tangled complex. Treatment with urea or heating at 50 C in 3 mm NaCl also produced this effect. Also under the conditions in which MLV-RNA was linear, RNA from Rous sarcoma virus also was linear, but Newcastle disease virus RNA and ribosomal RNA of rat liver had collapsed structures. The results indicated that the RNA of MLV, and perhaps other RNA-containing tumor viruses, has a specific unique conformation dependent upon hydrogen bonds.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kakefuda T,Bader JPdoi
10.1128/JVI.4.4.460-474.1969subject
Has Abstractpub_date
1969-10-01 00:00:00pages
460-74issue
4eissn
0022-538Xissn
1098-5514journal_volume
4pub_type
杂志文章abstract::Vesicular stomatitis virus (VSV) has been shown in laboratory studies to be effective against a variety of tumors, including malignant brain tumors. However, attenuation of VSV may be necessary to balance the potential toxicity toward normal cells, particularly when targeting brain tumors. Here we compared 10 recombin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02040-09
更新日期:2010-02-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV), a positive-strand RNA virus that belongs to the Arteriviridae family of Nidovirales, has been identified as the causative agent of PRRS. Nsp1alpha is the amino (N)-terminal protein in a polyprotein encoded by the PRRSV genome and is reported to be cru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02579-08
更新日期:2009-11-01 00:00:00
abstract::Viral replication depends on specific interactions with host factors. For example, poliovirus RNA replication requires association with intracellular membranes. Brefeldin A (BFA), which induces a major rearrangement of the cellular secretory apparatus, is a potent inhibitor of poliovirus RNA replication. Most aspects ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.7.3378-3386.2004
更新日期:2004-04-01 00:00:00
abstract:UNLABELLED:Susceptibility or resistance to prion infection in humans and animals depends on single prion protein (PrP) amino acid substitutions in the host, but the agent's modulating role has not been well investigated. Compared to disease incubation times in wild-type homozygous ARQ/ARQ (where each triplet represents...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02316-15
更新日期:2015-10-28 00:00:00
abstract::Extrachromosomal DNA purified from mink cells acutely infected with the Snyder-Theilen strain of feline sarcoma virus (FeSV) was digested with restriction endonucleases, and the DNA fragments were electrophoretically separated, transferred to a solid substrate, and hybridized with radiolabeled DNA transcripts compleme...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.3.860-875.1979
更新日期:1979-12-01 00:00:00
abstract::A mutation that disrupts the interaction between the NS2 protein of minute virus of mice and the nuclear export factor Crm1 results in a block to egress of mutant-generated full virions from the nucleus of infected murine cells. These mutants produce wild-type levels of monomer and dimer replicative DNA forms but are ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.7.3257-3266.2002
更新日期:2002-04-01 00:00:00
abstract::Replication of the plus-stranded RNA genome of hepatitis C virus (HCV) occurs in a membrane-bound replication complex consisting of viral and cellular proteins and viral RNA. NS5B, the RNA polymerase of HCV, is anchored to the membranes via a C-terminal 20-amino-acid-long hydrophobic domain, which is flanked on each s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02072-05
更新日期:2006-11-01 00:00:00
abstract::Structural proteins designated p10gag, p21gag, p8gag, p3gag, p27gag, and p14gag from the C3H strain of mouse mammary tumor virus (MMTV) were purified by reversed-phase high-pressure liquid chromatography. The N- and C-terminal amino acid sequences and amino acid composition of each protein were determined and compared...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.6.2543-2549.1989
更新日期:1989-06-01 00:00:00
abstract::Retrovirus infection is normally limited to cells within a specific host range which express a cognate receptor that is recognized by the product of the env gene. We describe retrovirus infection of cells outside of their normal host range when the infection is performed in the presence of a replication-defective aden...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1887-1894.1995
更新日期:1995-03-01 00:00:00
abstract::Phosphorylation of the murine mammary tumor virus (MuMTV) structural proteins was studied in an MuMTV-infected epithelial cell line derived from a BALB/cf C3H mouse mammary tumor. Immunoprecipitation of 32P-labeled cell extracts with monospecific anti-p27 serum revealed that phosphorylation occurred at the stage of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.1.241-247.1979
更新日期:1979-04-01 00:00:00
abstract::CD8+ T cells are the key cellular effectors mediating the clearance of hepatitis B virus (HBV) infections. However, early immunological events surrounding the priming of HBV-specific CD8+ T cell responses remain poorly understood. This study examined the importance of priming location and the relative contribution of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00920-18
更新日期:2018-10-12 00:00:00
abstract::Rift Valley fever virus strain MP-12 was generated by serial plaque passages of parental strain ZH548 12 times in MRC-5 cells in the presence of a chemical mutagen, 5-fluorouracil. As a result, MP-12 encoded 4, 9, and 10 mutations in the S, M, and L segments, respectively. Among them, mutations in the M and L segments...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02081-12
更新日期:2012-12-01 00:00:00
abstract::Latent Epstein-Barr virus (EBV) infection is associated with several lymphoproliferative disorders, including posttransplant lymphoma, Hodgkin's disease, and Burkitt's lymphoma, as well as nasopharyngeal carcinoma (NPC). Twenty-nine microRNAs (miRNAs) have been identified that are transcribed during latent infection f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00785-08
更新日期:2008-09-01 00:00:00
abstract::We developed a mouse model of Borna disease to facilitate immunopathogenesis research by adaptation of Borna disease virus to mice through serial passage in mouse brain tissue. Borna disease virus replication, antibody production, inflammation, and Borna disease expression in several different strains of mice were exa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.548-552.1993
更新日期:1993-01-01 00:00:00
abstract::Nine pre-early polypeptides have been detected after infection with bacteriophage T5 and 10 pre-early polypeptides have been detected after infection with bacteriophage BF23. Only about one-half of the coding capacity of the redundant ends of the phage DNA, which code for pre-early proteins, is needed for these 9 to 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.2.480-488.1977
更新日期:1977-05-01 00:00:00
abstract::Since HIV-1 replication is modulated at multiple stages by host cell factors, identification and characterization of those host cell factors are expected to contribute to the development of novel anti-HIV therapeutics. Previous studies showed that a C-terminally truncated cytosolic form of cleavage and polyadenylation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00124-13
更新日期:2013-07-01 00:00:00
abstract::Noroviruses are the causative agents of the majority of viral gastroenteritis outbreaks in humans. During the past 15 years, noroviruses of genotype GGII.4 have caused four epidemic seasons of viral gastroenteritis, during which four novel variants (termed epidemic variants) emerged and displaced the resident viruses....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00674-07
更新日期:2007-09-01 00:00:00
abstract::Klebsiella pneumoniae MirM7 is a wild-type strain which grows as cocci at pH 7 and above and as rods at pH 6.5 and below. Cultures of this strain and an auxotrophic derivative, MirM7b, have been found to undergo spontaneous lysis after purification from possible contaminating viruses. Lysates always contained two phag...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.3.772-785.1978
更新日期:1978-12-01 00:00:00
abstract::Poliovirus infection of HeLa cells results in a rapid inhibition of host protein synthesis by a mechanism that does not affect the translation of poliovirus RNA. It has been suggested that this virus-induced translational control results from inactivation of the cap-binding protein complex, and it has been shown that ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2480-2488.1987
更新日期:1987-08-01 00:00:00
abstract::FeLV-FAIDS, an immunodeficiency-inducing isolate of feline leukemia virus, is composed of a pathogenic but replication-defective genome (molecular clone 61C) and a replication-competent but non-immunodeficiency-inducing variant genome (molecular clone 61E). The chimeric virus EECC, composed of the 5' gag-pol of 61E fu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4338-4345.1990
更新日期:1990-09-01 00:00:00
abstract::A recent publication indicated that overexpression of Axl, a cellular receptor that negatively regulates Toll-like receptor signaling, enhanced the entry of viruses pseudotyped with the glycoprotein of lymphocytic choriomeningitis virus (LCMV) in vitro. In testing the biological relevance of these observations, we fou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03268-12
更新日期:2013-04-01 00:00:00
abstract::Recent findings suggest that most sexual transmission of human immunodeficiency virus type 1 (HIV-1) occurs during the acute phase of infection when viral replication is most intense. Here, we show that vaccine-elicited cellular immune responses can significantly reduce simian immunodeficiency virus levels in the seme...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01202-09
更新日期:2009-10-01 00:00:00
abstract::To examine the cell fusion activity of hepatitis C virus (HCV) envelope proteins (E1 and E2), we have established a sensitive cell fusion assay based on the activation of a reporter gene as described previously (O. Nussbaum, C. C. Broder, and E. A. Berger, J. Virol. 68:5411-5422, 1994). The chimeric HCV E1 and E2 prot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.5066-5074.2000
更新日期:2000-06-01 00:00:00
abstract:UNLABELLED:Neutralizing antibodies that target the hemagglutinin of influenza virus either inhibit binding of hemagglutinin to cellular receptors or prevent the low-pH-induced conformational change in hemagglutinin required for membrane fusion. In general, the former type of antibody binds to the globular head formed b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00420-14
更新日期:2014-07-01 00:00:00
abstract::For paramyxoviruses, entry requires a receptor-binding protein (hemagglutinin-neuraminidase [HN], H, or G) and a fusion protein (F). Like other class I viral fusion proteins, F is expressed as a prefusion metastable protein that undergoes a refolding event to induce fusion. HN binding to its receptor triggers F refold...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01191-09
更新日期:2009-11-01 00:00:00
abstract::Nipah virus (NiV) and Hendra virus (HeV) are emerging zoonotic viruses and the causative agents of severe respiratory disease and encephalitis in humans. Little is known about the mechanisms that govern the development of respiratory and neurological disease. Using a hamster model of lethal NiV and HeV infection, we d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00473-11
更新日期:2011-08-01 00:00:00
abstract::Ribosomes from ghost-treated Escherichia coli can form in vitro initiation complexes but cannot translate added template RNA because of a defect in the 30S subunit; 16S rRNA is degraded. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1310-1313.1974
更新日期:1974-11-01 00:00:00
abstract:UNLABELLED:Influenza B virus causes annual epidemics and, along with influenza A virus, accounts for substantial disease and economic burden throughout the world. Influenza B virus infects only humans and some marine mammals and is not responsible for pandemics, possibly due to a very low frequency of reassortment and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01576-15
更新日期:2015-12-01 00:00:00
abstract::Initiation of reverse transcription of human immunodeficiency virus type 1 (HIV-1) occurs by extension from the 3' end of a cellular tRNA complexed to the primer binding site (PBS) located near the 5' end of the viral RNA genome. Although the PBSs for all naturally occurring HIV-1 viruses are complementary to the 3'-t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.207-217.1997
更新日期:1997-01-01 00:00:00
abstract::The adenovirus type 5 mutant dl1520 was engineered previously to be completely defective for E1B-55K functions. Recently, this mutant (also known as ONYX-015) has been suggested to replicate preferentially in p53(-) and some p53(+) tumor cell lines but to be attenuated in primary cultured cells (C. Heise, A. Sampson-J...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.5333-5344.1999
更新日期:1999-07-01 00:00:00