Abstract:
:Extrachromosomal DNA purified from mink cells acutely infected with the Snyder-Theilen strain of feline sarcoma virus (FeSV) was digested with restriction endonucleases, and the DNA fragments were electrophoretically separated, transferred to a solid substrate, and hybridized with radiolabeled DNA transcripts complementary to different portions of the FeSV RNA genome. Major DNA species 8.4 and 5.0 kilobase pairs (kbp) long represent the linear, unintegrated proviruses of Snyder-Theilen feline leukemia virus and FeSV, respectively. Transfection experiments performed with electroeluted DNAs showed that the 8.4-kbp form led to the production of replicating nontransforming virus in mink and cat cells; in contrast, the 5.0-kbp DNA produced helper virus-independent foci of transformation in mouse NIH/3T3 cells and helper virus-dependent foci in mink cells at an efficiency comparable to that obtained with unfractionated extrachromosomal DNA. Sites of restriction endonuclease cleavage for six enzymes were oriented with respect to one another within the FeSV provirus. EcoRI recognized cleavage sites at 0.3 to 0.4 kbp from each terminus of FeSV DNA, reducing the 5.0-kbp DNA to molecules 4.3 kbp long; this enzyme excised a large internal proviral DNA fragment of corresponding size from the DNA of FeSV-transformed mink nonproducer cells. By using DNA transcripts complementary to different portions of the FeSV genome, sarcoma-specific sequences (the FeSV src gene) were positioned within 2.1 and 3.4 kbp from the 5' end of the proviral DNA with respect to the viral RNA genome. The src gene is flanked at both ends by sequences shared in common with feline leukemia virus. The localization of src sequences to this region suggests that a portion of an FeSV polyprotein which contains feline oncornavirus-associated cell membrane antigen (FOCMA-S) is the major product of this gene.
journal_name
J Viroljournal_title
Journal of virologyauthors
Sherr CJ,Fedele LA,Donner L,Turek LPdoi
10.1128/JVI.32.3.860-875.1979subject
Has Abstractpub_date
1979-12-01 00:00:00pages
860-75issue
3eissn
0022-538Xissn
1098-5514journal_volume
32pub_type
杂志文章abstract::We report the use of a cDNA microarray to monitor global transcriptional responses of the chestnut blight fungus, Cryphonectria parasitica, to infection by mild and severe isolates of virulence-attenuating hypoviruses that share 87 to 93% and 90 to 98% identity at the nucleotide and amino acid levels, respectively. In...
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2047-2056.1990
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doi:10.1128/JVI.28.1.154-170.1978
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pub_type: 杂志文章
doi:10.1128/JVI.66.10.6233-6236.1992
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pub_type: 杂志文章
doi:10.1128/JVI.71.11.8624-8631.1997
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.79.5.3107-3116.2005
更新日期:2005-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.15.9786-9798.2005
更新日期:2005-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.20.9412-9420.2000
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02349-08
更新日期:2009-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.4.698-700.1972
更新日期:1972-04-01 00:00:00
abstract::Control of HIV-1 replication following nonsterilizing HIV-1 vaccination could be achieved by vaccine-elicited CD8(+) T-cell-mediated antiviral activity. To date, neither the functional nor the phenotypic profiles of CD8(+) T cells capable of this activity are clearly understood; consequently, little is known regarding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00138-10
更新日期:2010-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.20.10799-10807.2003
更新日期:2003-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.62.1.91-99.1988
更新日期:1988-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1013-1018.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.3005-3012.1997
更新日期:1997-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.4.469-477.1971
更新日期:1971-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00646-08
更新日期:2008-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2007-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.12.6268-6286.2002
更新日期:2002-06-01 00:00:00
abstract::The phenotypes of a series of mutant human immunodeficiency virus type 1 proviruses with linker insertion and deletion mutations within the gag coding region were characterized. These mutants, with mutations in the matrix, capsid, and p2 coding regions, produced replication-defective virion particles with defects in t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8645-8652.1996
更新日期:1996-12-01 00:00:00
abstract::Murine monoclonal antibodies directed against the structural proteins p17 and p24 of human immunodeficiency virus type 1 were investigated in an epitope mapping system. Overlapping peptides consisting of 15 amino acids of the p17 and p24 protein, respectively, were used as competitors in an enzyme-linked immunosorbent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.8.3525-3528.1989
更新日期:1989-08-01 00:00:00
abstract::Retroviruses use RNA as their genetic material within viral particles and DNA (provirus) as their genetic material within cells. The rate of recombination during reverse transcription between two identical sequences within the same RNA molecule is very high. In this study, we have developed a sensitive system to study...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.16.7646-7650.2000
更新日期:2000-08-01 00:00:00