Abstract:
:Cell lines were generated by cotransfection of Vero cells with pSV2neo and a plasmid containing the herpes simplex virus type 1 (HSV-1) EcoRI D fragment (coordinates 0.086 to 0.194). One such cell line (S22) contained the genes for alkaline exonuclease and several uncharacterized functions. Three mutant isolates of HSV-1 strain KOS which grew on S22 cells but not on normal Vero cells were isolated and characterized. All three mutants (hr27, hr48, and hr156) were defective in the synthesis of viral DNA and late proteins when grown in nonpermissive Vero cells. Early gene expression in cells infected with these host range mutants appeared to be normal at the nonpermissive condition. The mutations were mapped by marker rescue to a 1.5-kilobase fragment (coordinates 0.145 to 0.155). The mutation of one of these mutants, hr27, was more finely mapped to an 800-base-pair region (coordinates 0.145 to 0.151). This position of these mutations is consistent with the map location of a putative 94-kilodalton polypeptide as determined by sequence analysis (D. McGeoch, personal communication). Complementation studies demonstrated that these mutants formed a new complementation group, designated 1-36. The results presented in this report indicate that the 94-kilodalton gene product affected by these mutations may have a direct role in viral DNA synthesis.
journal_name
J Viroljournal_title
Journal of virologyauthors
Carmichael EP,Kosovsky MJ,Weller SKdoi
10.1128/JVI.62.1.91-99.1988subject
Has Abstractpub_date
1988-01-01 00:00:00pages
91-9issue
1eissn
0022-538Xissn
1098-5514journal_volume
62pub_type
杂志文章abstract::T-antigen-induced DNA distortion was studied in a series of simian virus 40 (SV40) plasmid constructs whose relative replication efficiency ranges from 0.2 to 36. Bending was detected in the wild-type SV40 regulatory region consisting of three copies of the GC-rich 21-bp repeat but not in constructs with only one or t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10651-10657.2003
更新日期:2003-10-01 00:00:00
abstract:UNLABELLED:The disease mechanisms associated with onset and secondary effects of rotavirus (RV) diarrhea remain to be determined and may not be identical. In this study, we investigated whether onset of RV diarrhea is associated with increased intestinal permeability and/or motility. To study the transit time, fluoresc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02927-13
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1452-1461.2003
更新日期:2003-01-01 00:00:00
abstract:UNLABELLED:Japanese encephalitis virus (JEV) membrane (M) protein plays important structural roles in the processes of fusion and maturation of progeny virus during cellular infection. The M protein is anchored in the viral membrane, and its ectodomain is composed of a flexible N-terminal loop and a perimembrane helix....
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pub_type: 杂志文章
doi:10.1128/JVI.01176-15
更新日期:2015-12-09 00:00:00
abstract::The high diversity of HLA binding preferences has been driven by the sequence diversity of short segments of relevant pathogenic proteins presented by HLA molecules to the immune system. To identify possible commonalities in HLA binding preferences, we quantify these using a novel measure termed "targeting efficiency,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01966-10
更新日期:2011-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.1998-2005.1999
更新日期:1999-03-01 00:00:00
abstract::We identified the domains of CD26 involved in the binding of Middle East respiratory syndrome coronavirus (MERS-CoV) using distinct clones of anti-CD26 monoclonal antibodies (MAbs). One clone, named 2F9, almost completely inhibited viral entry. The humanized anti-CD26 MAb YS110 also significantly inhibited infection. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02448-13
更新日期:2013-12-01 00:00:00
abstract::Although shrews are one of the largest groups of mammals, little is known about their role in the evolution and transmission of viral pathogens, including coronaviruses (CoVs). We captured 266 Asian house shrews (Suncus murinus) in Jiangxi and Zhejiang Provinces, China, during 2013 to 2015. CoV RNA was detected in 24 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00764-17
更新日期:2017-08-10 00:00:00
abstract::Necroptosis, a regulated form of necrotic cell death, requires the activation of the RIP3 kinase. Here, we identify that infection of host cells with reovirus can result in necroptosis. We find that necroptosis requires sensing of the genomic RNA within incoming virus particles via cytoplasmic RNA sensors to produce t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02404-16
更新日期:2017-02-28 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03388-12
更新日期:2013-04-01 00:00:00
abstract::Dengue virus (DEN) causes dengue fever and dengue hemorrhagic fever/dengue shock syndrome, which are major public health problems worldwide. The immune factors that control DEN infection or contribute to severe disease are neither well understood nor easy to examine in humans. In this study, we used wild-type and cong...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.6.2701-2710.2004
更新日期:2004-03-01 00:00:00
abstract::The alphaherpesvirus proteins UL31 and UL34 and their homologues in other herpesvirus subfamilies cooperate at the nuclear membrane in the export of nascent herpesvirus capsids. We studied the respective betaherpesvirus proteins M53 and M50 in mouse cytomegalovirus (MCMV). Recently, we established a random approach to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00681-10
更新日期:2010-09-01 00:00:00
abstract:UNLABELLED:Viruses that spread systemically from a peripheral site of infection cause morbidity and mortality in the human population. Innate myeloid cells, including monocytes, macrophages, monocyte-derived dendritic cells (mo-DC), and dendritic cells (DC), respond early during viral infection to control viral replica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01024-15
更新日期:2015-10-01 00:00:00
abstract::The complete nucleotide sequence of the genome of classical swine fever virus (CSFV) strain Alfort/187 was determined from three cDNA libraries constructed by cloning of DNA fragments obtained from independent sets of reverse transcription and PCR. The cDNA fragments were then assembled and inserted downstream of a T7...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3478-3487.1996
更新日期:1996-06-01 00:00:00
abstract::The protein (p59rel) encoded by the transforming gene of reticuloendotheliosis virus strain T (REV-T) has been identified in REV-T-transformed avian lymphoid cells by using antisera raised against synthetic peptides whose sequences were derived from three nonoverlapping regions of v-rel (N. R. Rice, T. D. Copeland, S....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.1.120-126.1986
更新日期:1986-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.1.175-186.1979
更新日期:1979-10-01 00:00:00
abstract::Herpes simplex virus type 1 packages its DNA genome into a precursor capsid, referred to as the procapsid. Of the three capsid-associated DNA-packaging proteins, UL17, UL25, and UL6, only UL17 and UL6 appear to be components of the procapsid, with UL25 being added subsequently. To determine whether the association of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.5.2118-2126.2006
更新日期:2006-03-01 00:00:00
abstract::We have constructed new murine leukemia virus (MLV)-based vectors (TIN vectors) which, following integration, contain human immunodeficiency virus (HIV) type 1 U3 and R sequences in place of the MLV U3 and R regions. This provides, for the first time, single transcriptional unit retroviral vectors under the control of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.8234-8240.1996
更新日期:1996-11-01 00:00:00
abstract::Retroviral genomes are dimeric, comprised of two sense-strand RNAs linked at their 5' ends by noncovalent base pairing and tertiary interactions. Viral maturation involves large-scale morphological changes in viral proteins and in genomic RNA dimer structures to yield infectious virions. Structural studies have largel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01602-09
更新日期:2010-01-01 00:00:00
abstract::Hepatitis E virus replicons containing the neomycin resistance gene expressed from open reading frames (ORFs) 2 and 3 were transfected into Huh-7 cells, and stable cell lines containing functional replicons were selected by constant exposure to G418 sulfate. Northern blot analyses detected full-length replicon RNA and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00046-06
更新日期:2006-06-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent of the aggressive and fatal disease adult T-cell leukemia. Previous studies have demonstrated that the HTLV-1-encoded Tax protein inhibits the function of tumor suppressor p53 through a Tax-induced NF-kappaB pathway. Given these attributes, we were int...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00690-08
更新日期:2008-09-01 00:00:00
abstract::Herpesvirus lytic DNA replication requires both the cis-acting element, the origin, and trans-acting factors, including virally encoded origin-binding protein, DNA replication enzymes, and auxiliary factors. Two lytic DNA replication origins (ori-Lyt) of Kaposi's sarcoma-associated herpesvirus (KSHV) have been identif...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01319-07
更新日期:2008-03-01 00:00:00
abstract::The rotavirus nonstructural phosphoprotein NSP5 is encoded by a gene in RNA segment 11. Immunofluorescence analysis of fixed cells showed that NSP5 polypeptides remained confined to viroplasms even at a late stage when provirions migrated from these structures. When NSP5 was expressed in COS-7 cells in the absence of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6398-6405.1998
更新日期:1998-08-01 00:00:00
abstract::Y-box binding protein YB-1 is a member of a family of DNA and RNA binding proteins which have been shown to affect gene expression at both the transcriptional and translational levels. We have previously shown that YB-1 modulates transcription from the promoters of the ubiquitous human polyomavirus JC virus (JCV). Her...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.12.10146-10157.1999
更新日期:1999-12-01 00:00:00
abstract::We report on the use of spectral map analysis of the inter- and intraclade neutralization data of 14 sera of human immunodeficiency virus type 1 (HIV-1)-infected individuals and 16 primary isolates, representing genetic clades A to H in group M and group O. This multivariate analysis has been used previously to study ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6235-6243.1996
更新日期:1996-09-01 00:00:00
abstract::Cowpox virus (CPXV) belongs to the genus Orthopoxvirus in the Poxviridae family. It infects a broad range of vertebrates and can cause zoonotic infections. CPXV has the largest genome among the orthopoxviruses and is therefore considered to have the most complete set of genes of all members of the genus. Since CPXV ha...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02385-13
更新日期:2014-01-01 00:00:00
abstract::Inhibition of histone deacetylase (HDAC) activity induces growth arrest, differentiation, and, in certain cell types, apoptosis. FR901228, FK228, or depsipeptide, is an HDAC inhibitor effective in T-cell lymphomas. Adult T-cell leukemia (ATL) is caused by human T-cell leukemia virus type 1 (HTLV-1) and remains incurab...
journal_title:Journal of virology
pub_type: 杂志文章,收录出版
doi:10.1128/jvi.78.9.4582-4590.2004
更新日期:2004-05-01 00:00:00
abstract::Mutants of polyomavirus with altered host specificities were isolated in undifferentiated C2 myoblast cells (L. Ricci, R. Maione, C. Passananti, A. Felsani, and P. Amati, J. Virol. 66:7153-7158, 1992). The mutations responsible for this phenotype belonged to either of the two classes: a large duplication of the enhanc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1196-1199.1994
更新日期:1994-02-01 00:00:00
abstract::Phosphorylation of viral proteins plays important roles in the influenza A virus (IAV) life cycle. By using mass spectrometry, we identified tyrosine 132 (Y132) as a phosphorylation site of the matrix protein (M1) of the influenza virus A/WSN/1933(H1N1). Phosphorylation at this site is essential to the process of viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03024-12
更新日期:2013-06-01 00:00:00
abstract::A fragment of chicken DNA containing the left long terminal repeat of endogenous retrovirus ev-2 and flanking cellular sequences has been molecularly cloned and analyzed. Comparison with sequence data from the analogous regions of ev-1 and Rous-associated virus-0 viral DNA reveals similarities among flanking regions o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.2.868-871.1983
更新日期:1983-02-01 00:00:00